Restriction map for
pF1KE0603
(Circular) (Six-base) MAPSORT of: pF1KE0603.seq Check: 8400 from: 1 to: 3581
pF1KE0603 3581 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 619 619
Size: 3581
Acc65I G'GTAC_C
Cuts at: 597 597
Size: 3581
AclI AA'CG_TT
Cuts at: 3421 3421
Size: 3581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 235 270 284 1406 1838 2585 2853 145
Size: 90 35 14 1122 432 747 268 873
Fragments arranged by size:
1122 873 747 432 268 90 35 14
AflIII A'CryG_T
Cuts at: 418 933 2037 3014 418
Size: 515 1104 977 985
Fragments arranged by size:
1104 985 977 515
AgeI A'CCGG_T
Cuts at: 1969 1969
Size: 3581
AlwNI CAG_nnn'CTG
Cuts at: 886 2453 886
Size: 1567 2014
ApaLI G'TGCA_C
Cuts at: 2351 2351
Size: 3581
ApoI r'AATT_y
Cuts at: 585 2934 3190 585
Size: 2349 256 976
Fragments arranged by size:
2349 976 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3581
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3581
AvaI C'yCGr_G
Cuts at: 601 601
Size: 3581
BamHI G'GATC_C
Cuts at: 606 606
Size: 3581
BanI G'GyrC_C
Cuts at: 597 1011 1259 1294 597
Size: 414 248 35 2884
Fragments arranged by size:
2884 414 248 35
BanII G_rGCy'C
Cuts at: 595 1625 595
Size: 1030 2551
BbeI G_GCGC'C
Cuts at: 1015 1263 1015
Size: 248 3333
BciVI GTATCCnnnnn_n'
Cuts at: 1479 2240 3581 1479
Size: 761 1341 1479
Fragments arranged by size:
1479 1341 761
BclI T'GATC_A
Cuts at: 1101 1101
Size: 3581
BglII A'GATC_T
Cuts at: 1096 3133 1096
Size: 2037 1544
BlpI GC'TnA_GC
Cuts at: 157 693 157
Size: 536 3045
Bme1580I G_kGCm'C
Cuts at: 1206 1299 2355 1206
Size: 93 1056 2432
Fragments arranged by size:
2432 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 459 793 874 1209 3353 459
Size: 334 81 335 2144 687
Fragments arranged by size:
2144 687 335 334 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 1636 2128 2426 757
Size: 879 492 298 1912
Fragments arranged by size:
1912 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3285 3285
Size: 3581
BsaAI yAC'GTr
Cuts at: 150 934 150
Size: 784 2797
BsaBI GATnn'nnATC
Cuts at: 6 518 1095 3132 6
Size: 512 577 2037 455
Fragments arranged by size:
2037 577 512 455
BsaHI Gr'CG_yC
Cuts at: 1012 1260 1962 3458 1012
Size: 248 702 1496 1135
Fragments arranged by size:
1496 1135 702 248
BsaWI w'CCGG_w
Cuts at: 770 1291 1969 2243 2390 2801 770
Size: 521 678 274 147 411 1550
Fragments arranged by size:
1550 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 560 560
Size: 3581
BseYI C'CCAG_C
Cuts at: 538 1006 1764 2341 2837 538
Size: 468 758 577 496 1282
Fragments arranged by size:
1282 758 577 496 468
BsiEI CG_ry'CG
Cuts at: 104 486 1123 1169 2377 104
Size: 382 637 46 1208 1308
Fragments arranged by size:
1308 1208 637 382 46
BsiHKAI G_wGCw'C
Cuts at: 595 1373 1563 2355 595
Size: 778 190 792 1821
Fragments arranged by size:
1821 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 595 1206 1299 1373 1563 1625 2355 595
Size: 611 93 74 190 62 730 1821
Fragments arranged by size:
1821 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2801 2801
Size: 3581
BspHI T'CATG_A
Cuts at: 2757 2757
Size: 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 631 845 1147 1528 631
Size: 214 302 381 2684
Fragments arranged by size:
2684 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1873 1927 3411 3576 50
Size: 1823 54 1484 165 55
Fragments arranged by size:
1823 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1493 1493
Size: 3581
BsrFI r'CCGG_y
Cuts at: 1579 1760 1969 1579
Size: 181 209 3191
Fragments arranged by size:
3191 209 181
BssSI C'ACGA_G
Cuts at: 1852 2210 1852
Size: 358 3223
BstAPI GCAn_nnn'nTGC
Cuts at: 958 958
Size: 3581
BstBI TT'CG_AA
Cuts at: 1942 1942
Size: 3581
BstYI r'GATC_y
Cuts at: 1 606 1073 1096 1431 1677 2689 2775 2787
Size: 605 467 23 335 246 1012 86 12
Cuts at: 2787 3133 1
Size: 346 449
Fragments arranged by size:
1012 605 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 486 486
Size: 3581
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1531 1712 1875 1531
Size: 181 163 3237
Fragments arranged by size:
3237 181 163
BtsI GCAGTG_nn'
Cuts at: 824 824
Size: 3581
DraI TTT'AAA
Cuts at: 580 580
Size: 3581
DrdI GACnn_nn'nnGTC
Cuts at: 1287 2145 1287
Size: 858 2723
EaeI y'GGCC_r
Cuts at: 483 1166 1340 1731 1758 3103 483
Size: 683 174 391 27 1345 961
Fragments arranged by size:
1345 961 683 391 174 27
EagI C'GGCC_G
Cuts at: 483 1166 483
Size: 683 2898
EarI CTCTTCn'nnn_
Cuts at: 289 1604 1814 289
Size: 1315 210 2056
Fragments arranged by size:
2056 1315 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1789 2099 2245 3391 1789
Size: 310 146 1146 1979
Fragments arranged by size:
1979 1146 310 146
EcoICRI GAG'CTC
Cuts at: 593 593
Size: 3581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 235 270 284 1406 1838 2585 2853 145
Size: 90 35 14 1122 432 747 268 873
Fragments arranged by size:
1122 873 747 432 268 90 35 14
EcoNI CCTnn'n_nnAGG
Cuts at: 124 124
Size: 3581
EcoO109I rG'GnC_Cy
Cuts at: 720 720
Size: 3581
EcoRI G'AATT_C
Cuts at: 585 585
Size: 3581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1600 1600
Size: 3581
FspI TGC'GCA
Cuts at: 136 1362 136
Size: 1226 2355
HaeII r_GCGC'y
Cuts at: 133 1015 1263 2285 3264 133
Size: 882 248 1022 979 450
Fragments arranged by size:
1022 979 882 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1423 1453 1455 1485 1423
Size: 30 2 30 3519
Fragments arranged by size:
3519 30 30 2
HincII GTy'rAC
Cuts at: 620 620
Size: 3581
KasI G'GCGC_C
Cuts at: 1011 1259 1011
Size: 248 3333
KpnI G_GTAC'C
Cuts at: 601 601
Size: 3581
MluI A'CGCG_T
Cuts at: 418 3014 418
Size: 2596 985
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 219 2252 2436 219
Size: 2033 184 1364
Fragments arranged by size:
2033 1364 184
MscI TGG'CCA
Cuts at: 1342 1342
Size: 3581
MslI CAynn'nnrTG
Cuts at: 1570 1697 1570
Size: 127 3454
MspA1I CmG'CkG
Cuts at: 692 1006 1366 2379 2624 692
Size: 314 360 1013 245 1649
Fragments arranged by size:
1649 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1762 1762
Size: 3581
NarI GG'CG_CC
Cuts at: 1012 1260 1012
Size: 248 3333
NgoMIV G'CCGG_C
Cuts at: 1760 1760
Size: 3581
NspI r_CATG'y
Cuts at: 350 634 1134 2041 350
Size: 284 500 907 1890
Fragments arranged by size:
1890 907 500 284
PciI A'CATG_T
Cuts at: 2037 2037
Size: 3581
PmeI GTTT'AAAC
Cuts at: 580 580
Size: 3581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2720 2752 2720
Size: 32 3549
PstI C_TGCA'G
Cuts at: 628 1313 628
Size: 685 2896
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3581
PvuII CAG'CTG
Cuts at: 1006 1366 1006
Size: 360 3221
RsrII CG'GwC_CG
Cuts at: 1776 1776
Size: 3581
SacI G_AGCT'C
Cuts at: 595 595
Size: 3581
SalI G'TCGA_C
Cuts at: 618 618
Size: 3581
SapI GCTCTTCn'nnn_
Cuts at: 1604 1814 1604
Size: 210 3371
SbfI CC_TGCA'GG
Cuts at: 628 628
Size: 3581
ScaI AGT'ACT
Cuts at: 3098 3098
Size: 3581
SexAI A'CCwGG_T
Cuts at: 572 572
Size: 3581
SfcI C'TryA_G
Cuts at: 33 624 1309 2302 2493 33
Size: 591 685 993 191 1121
Fragments arranged by size:
1121 993 685 591 191
SfoI GGC'GCC
Cuts at: 1013 1261 1013
Size: 248 3333
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3581
SmaI CCC'GGG
Cuts at: 603 603
Size: 3581
SmlI C'TyrA_G
Cuts at: 736 1651 2143 2405 736
Size: 915 492 262 1912
Fragments arranged by size:
1912 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3581
SphI G_CATG'C
Cuts at: 634 1134 634
Size: 500 3081
SspI AAT'ATT
Cuts at: 370 370
Size: 3581
StyI C'CwwG_G
Cuts at: 715 1069 715
Size: 354 3227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 446 1164 1297 1756 1965 446
Size: 718 133 459 209 2062
Fragments arranged by size:
2062 718 459 209 133
TatI w'GTAC_w
Cuts at: 3096 3096
Size: 3581
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 3549
Tth111I GACn'n_nGTC
Cuts at: 1378 1378
Size: 3581
XbaI T'CTAG_A
Cuts at: 612 612
Size: 3581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1076 1076
Size: 3581
XmaI C'CCGG_G
Cuts at: 601 601
Size: 3581
XmnI GAAnn'nnTTC
Cuts at: 246 246
Size: 3581
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI