Restriction map for
pF1KE0604
(Circular) (Six-base) MAPSORT of: pF1KE0604.seq Check: 3376 from: 1 to: 4190
pF1KE0604 4190 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1228 1228
Size: 4190
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 749 2015 2447 3194 3462 271
Size: 478 1266 432 747 268 999
Fragments arranged by size:
1266 999 747 478 432 268
AflIII A'CryG_T
Cuts at: 1542 2646 3623 1542
Size: 1104 977 2109
Fragments arranged by size:
2109 1104 977
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 747 1495 3062 747
Size: 748 1567 1875
Fragments arranged by size:
1875 1567 748
ApaLI G'TGCA_C
Cuts at: 2960 2960
Size: 4190
ApoI r'AATT_y
Cuts at: 213 292 595 751 884 1194 3543 3799 213
Size: 79 303 156 133 310 2349 256 604
Fragments arranged by size:
2349 604 310 303 256 156 133 79
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 1210 1210
Size: 4190
BamHI G'GATC_C
Cuts at: 1215 1215
Size: 4190
BanI G'GyrC_C
Cuts at: 1149 1206 1620 1868 1903 1149
Size: 57 414 248 35 3436
Fragments arranged by size:
3436 414 248 57 35
BanII G_rGCy'C
Cuts at: 420 1204 2234 420
Size: 784 1030 2376
Fragments arranged by size:
2376 1030 784
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4156
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 828 1710 828
Size: 882 3308
BglII A'GATC_T
Cuts at: 1705 3742 1705
Size: 2037 2153
BlpI GC'TnA_GC
Cuts at: 1302 1302
Size: 4190
Bme1580I G_kGCm'C
Cuts at: 1815 1908 2964 1815
Size: 93 1056 3041
Fragments arranged by size:
3041 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 414 1402 1483 1818 3962 414
Size: 988 81 335 2144 642
Fragments arranged by size:
2144 988 642 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 338 908 1055 338
Size: 570 147 3473
Fragments arranged by size:
3473 570 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2737 3035 1366
Size: 879 492 298 2521
Fragments arranged by size:
2521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 1543 1543
Size: 4190
BsaBI GATnn'nnATC
Cuts at: 6 827 1704 3741 6
Size: 821 877 2037 455
Fragments arranged by size:
2037 877 821 455
BsaHI Gr'CG_yC
Cuts at: 1621 1869 2571 4067 1621
Size: 248 702 1496 1744
Fragments arranged by size:
1744 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1379 1900 2578 2852 2999 3410 1379
Size: 521 678 274 147 411 2159
Fragments arranged by size:
2159 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 117 147 117
Size: 30 4160
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 120 821 120
Size: 701 3489
BseYI C'CCAG_C
Cuts at: 118 346 1615 2373 2950 3446 118
Size: 228 1269 758 577 496 862
Fragments arranged by size:
1269 862 758 577 496 228
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 537 924 994 1204 1982 2172 2964 537
Size: 387 70 210 778 190 792 1763
Fragments arranged by size:
1763 792 778 387 210 190 70
Bsp1286I G_dGCh'C
Cuts at: 420 537 924 994 1204 1815 1908 1982 2172
Size: 117 387 70 210 611 93 74 190
Cuts at: 2172 2234 2964 420
Size: 62 730 1646
Fragments arranged by size:
1646 730 611 387 210 190 117 93
74 70 62
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 3366 3366
Size: 4190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1240 1454 1756 2137 1240
Size: 214 302 381 3293
Fragments arranged by size:
3293 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2482 2536 4020 4185 50
Size: 2432 54 1484 165 55
Fragments arranged by size:
2432 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2102 2102
Size: 4190
BsrFI r'CCGG_y
Cuts at: 2188 2369 2578 2188
Size: 181 209 3800
Fragments arranged by size:
3800 209 181
BssSI C'ACGA_G
Cuts at: 2461 2819 2461
Size: 358 3832
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4190
BstBI TT'CG_AA
Cuts at: 2551 2551
Size: 4190
BstXI CCAn_nnnn'nTGG
Cuts at: 1146 1146
Size: 4190
BstYI r'GATC_y
Cuts at: 1 1215 1682 1705 2040 2286 3298 3384 3396
Size: 1214 467 23 335 246 1012 86 12
Cuts at: 3396 3742 1
Size: 346 449
Fragments arranged by size:
1214 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 149 788 978 149
Size: 639 190 3361
Fragments arranged by size:
3361 639 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2140 2321 2484 2140
Size: 181 163 3846
Fragments arranged by size:
3846 181 163
BtsI GCAGTG_nn'
Cuts at: 1433 1433
Size: 4190
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 1775 1949 2340 2367 3712 1775
Size: 174 391 27 1345 2253
Fragments arranged by size:
2253 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 905 2213 2423 905
Size: 1308 210 2672
Fragments arranged by size:
2672 1308 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1202 1202
Size: 4190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 338 749 908 1055 2015 2447 3194 3462
Size: 67 411 159 147 960 432 747 268
Cuts at: 3462 271
Size: 999
Fragments arranged by size:
999 960 747 432 411 268 159 147
67
EcoNI CCTnn'n_nnAGG
Cuts at: 148 148
Size: 4190
EcoO109I rG'GnC_Cy
Cuts at: 1329 1329
Size: 4190
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 4190
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 1624 1872 2894 3873 1624
Size: 248 1022 979 1941
Fragments arranged by size:
1941 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 259 291 757 789 1039 1071 2032 2062 2064
Size: 32 466 32 250 32 961 30 2
Cuts at: 2064 2094 259
Size: 30 2355
Fragments arranged by size:
2355 961 466 250 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 868 1229 868
Size: 361 3829
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MfeI C'AATT_G
Cuts at: 431 431
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2861 3045 2861
Size: 184 4006
MscI TGG'CCA
Cuts at: 1951 1951
Size: 4190
MslI CAynn'nnrTG
Cuts at: 2179 2306 2179
Size: 127 4063
MspA1I CmG'CkG
Cuts at: 747 1301 1615 1975 2988 3233 747
Size: 554 314 360 1013 245 1704
Fragments arranged by size:
1704 1013 554 360 314 245
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NspI r_CATG'y
Cuts at: 1243 1743 2650 1243
Size: 500 907 2783
Fragments arranged by size:
2783 907 500
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PstI C_TGCA'G
Cuts at: 1237 1922 1237
Size: 685 3505
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 747 1615 1975 747
Size: 868 360 2962
Fragments arranged by size:
2962 868 360
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 1204 1204
Size: 4190
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 2213 2423 2213
Size: 210 3980
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 3707 3707
Size: 4190
SfcI C'TryA_G
Cuts at: 33 612 1233 1918 2911 3102 33
Size: 579 621 685 993 191 1121
Fragments arranged by size:
1121 993 685 621 579 191
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 1345 2260 2752 3014 1345
Size: 915 492 262 2521
Fragments arranged by size:
2521 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StyI C'CwwG_G
Cuts at: 1324 1678 1324
Size: 354 3836
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1773 1906 2365 2574 1773
Size: 133 459 209 3389
Fragments arranged by size:
3389 459 209 133
TatI w'GTAC_w
Cuts at: 1030 3705 1030
Size: 2675 1515
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 117 149 3707 3739 117
Size: 32 3558 32 568
Fragments arranged by size:
3558 568 32 32
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4190
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1685 1685
Size: 4190
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
XmnI GAAnn'nnTTC
Cuts at: 896 896
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I BtgI ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI