Restriction map for
pF1KE0606
(Circular) (Six-base) MAPSORT of: pF1KE0606.seq Check: 9545 from: 1 to: 4472
pF1KE0606 4472 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 837 1167 837
Size: 330 4142
AccI GT'mk_AC
Cuts at: 797 1510 797
Size: 713 3759
Acc65I G'GTAC_C
Cuts at: 1488 1488
Size: 4472
AclI AA'CG_TT
Cuts at: 4312 4312
Size: 4472
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 2297 2729 3476 3744 513
Size: 1784 432 747 268 1241
Fragments arranged by size:
1784 1241 747 432 268
AfeI AGC'GCT
Cuts at: 505 505
Size: 4472
AflII C'TTAA_G
Cuts at: 1335 1335
Size: 4472
AflIII A'CryG_T
Cuts at: 1824 2928 3905 1824
Size: 1104 977 2391
Fragments arranged by size:
2391 1104 977
AgeI A'CCGG_T
Cuts at: 2860 2860
Size: 4472
AleI CACnn'nnGTG
Cuts at: 274 274
Size: 4472
AlwNI CAG_nnn'CTG
Cuts at: 137 279 755 1182 1777 3344 137
Size: 142 476 427 595 1567 1265
Fragments arranged by size:
1567 1265 595 476 427 142
ApaLI G'TGCA_C
Cuts at: 3242 3242
Size: 4472
ApoI r'AATT_y
Cuts at: 1476 3825 4081 1476
Size: 2349 256 1867
Fragments arranged by size:
2349 1867 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4472
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
AvaI C'yCGr_G
Cuts at: 126 169 712 1390 1492 126
Size: 43 543 678 102 3106
Fragments arranged by size:
3106 678 543 102 43
BamHI G'GATC_C
Cuts at: 1497 1497
Size: 4472
BanI G'GyrC_C
Cuts at: 784 1298 1488 1902 2150 2185 784
Size: 514 190 414 248 35 3071
Fragments arranged by size:
3071 514 414 248 190 35
BanII G_rGCy'C
Cuts at: 874 1364 1406 1486 2516 874
Size: 490 42 80 1030 2830
Fragments arranged by size:
2830 1030 490 80 42
BbeI G_GCGC'C
Cuts at: 788 1302 1906 2154 788
Size: 514 604 248 3106
Fragments arranged by size:
3106 604 514 248
BbvCI CC'TCA_GC
Cuts at: 904 1111 904
Size: 207 4265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1141 1175 1141
Size: 34 4438
BciVI GTATCCnnnnn_n'
Cuts at: 286 2370 3131 4472 286
Size: 2084 761 1341 286
Fragments arranged by size:
2084 1341 761 286
BclI T'GATC_A
Cuts at: 153 221 1992 153
Size: 68 1771 2633
Fragments arranged by size:
2633 1771 68
BglI GCCn_nnn'nGGC
Cuts at: 128 604 869 128
Size: 476 265 3731
Fragments arranged by size:
3731 476 265
BglII A'GATC_T
Cuts at: 1987 4024 1987
Size: 2037 2435
BlpI GC'TnA_GC
Cuts at: 1584 1584
Size: 4472
Bme1580I G_kGCm'C
Cuts at: 659 889 2097 2190 3246 659
Size: 230 1208 93 1056 1885
Fragments arranged by size:
1885 1208 1056 230 93
BmgBI CAC'GTC
Cuts at: 410 410
Size: 4472
BmrI ACTGGGnnnn_n'
Cuts at: 1359 1371 1684 1765 2100 4244 1359
Size: 12 313 81 335 2144 1587
Fragments arranged by size:
2144 1587 335 313 81 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1425 2839 1425
Size: 1414 3058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 672 732 672
Size: 60 4412
Bpu10I CC'TnA_GC
Cuts at: 904 1111 904
Size: 207 4265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1648 2527 3019 3317 1648
Size: 879 492 298 2803
Fragments arranged by size:
2803 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 676 709 4176 676
Size: 33 3467 972
Fragments arranged by size:
3467 972 33
BsaAI yAC'GTr
Cuts at: 483 1825 483
Size: 1342 3130
BsaBI GATnn'nnATC
Cuts at: 6 196 1986 4023 6
Size: 190 1790 2037 455
Fragments arranged by size:
2037 1790 455 190
BsaHI Gr'CG_yC
Cuts at: 785 1299 1903 2151 2853 4349 785
Size: 514 604 248 702 1496 908
Fragments arranged by size:
1496 908 702 604 514 248
BsaWI w'CCGG_w
Cuts at: 1661 2182 2860 3134 3281 3692 1661
Size: 521 678 274 147 411 2441
Fragments arranged by size:
2441 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 107 137 879 909 107
Size: 30 742 30 3670
Fragments arranged by size:
3670 742 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1072 1072
Size: 4472
BseYI C'CCAG_C
Cuts at: 118 658 1897 2655 3232 3728 118
Size: 540 1239 758 577 496 862
Fragments arranged by size:
1239 862 758 577 540 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 463 1195 1291 421
Size: 42 732 96 3602
Fragments arranged by size:
3602 732 96 42
BsiEI CG_ry'CG
Cuts at: 104 2014 2060 3268 104
Size: 1910 46 1208 1308
Fragments arranged by size:
1910 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 854 1486 2264 2454 3246 854
Size: 632 778 190 792 2080
Fragments arranged by size:
2080 792 778 632 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1090 1090
Size: 4472
Bsp1286I G_dGCh'C
Cuts at: 659 854 874 889 1364 1406 1486 2097 2190
Size: 195 20 15 475 42 80 611 93
Cuts at: 2190 2264 2454 2516 3246 659
Size: 74 190 62 730 1885
Fragments arranged by size:
1885 730 611 475 195 190 93 80
74 62 42 20 15
BspEI T'CCGG_A
Cuts at: 3692 3692
Size: 4472
BspHI T'CATG_A
Cuts at: 854 3648 854
Size: 2794 1678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 837 1167 1522 1736 2038 2419 837
Size: 330 355 214 302 381 2890
Fragments arranged by size:
2890 381 355 330 302 214
BsrBI CCG'CTC
Cuts at: 50 686 1131 2764 2818 4302 4467 50
Size: 636 445 1633 54 1484 165 55
Fragments arranged by size:
1633 1484 636 445 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2384 2384
Size: 4472
BsrFI r'CCGG_y
Cuts at: 2470 2651 2860 2470
Size: 181 209 4082
Fragments arranged by size:
4082 209 181
BsrGI T'GTAC_A
Cuts at: 1136 1136
Size: 4472
BssSI C'ACGA_G
Cuts at: 703 802 1216 2743 3101 703
Size: 99 414 1527 358 2074
Fragments arranged by size:
2074 1527 414 358 99
BstAPI GCAn_nnn'nTGC
Cuts at: 137 1849 137
Size: 1712 2760
BstBI TT'CG_AA
Cuts at: 2833 2833
Size: 4472
BstEII G'GTnAC_C
Cuts at: 267 588 267
Size: 321 4151
BstYI r'GATC_y
Cuts at: 1 1497 1964 1987 2322 2568 3580 3666 3678
Size: 1496 467 23 335 246 1012 86 12
Cuts at: 3678 4024 1
Size: 346 449
Fragments arranged by size:
1496 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 961 961
Size: 4472
BtgI C'CryG_G
Cuts at: 107 371 626 107
Size: 264 255 3953
Fragments arranged by size:
3953 264 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 495 2422 2603 2766 495
Size: 1927 181 163 2201
Fragments arranged by size:
2201 1927 181 163
BtsI GCAGTG_nn'
Cuts at: 138 607 1715 138
Size: 469 1108 2895
Fragments arranged by size:
2895 1108 469
DraI TTT'AAA
Cuts at: 1471 1471
Size: 4472
DraIII CAC_nnn'GTG
Cuts at: 324 324
Size: 4472
DrdI GACnn_nn'nnGTC
Cuts at: 2178 3036 2178
Size: 858 3614
EaeI y'GGCC_r
Cuts at: 182 878 983 1028 2057 2231 2622 2649 3994
Size: 696 105 45 1029 174 391 27 1345
Cuts at: 3994 182
Size: 660
Fragments arranged by size:
1345 1029 696 660 391 174 105 45
27
EagI C'GGCC_G
Cuts at: 2057 2057
Size: 4472
EarI CTCTTCn'nnn_
Cuts at: 454 917 1291 2495 2705 454
Size: 463 374 1204 210 2221
Fragments arranged by size:
2221 1204 463 374 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 585 2680 2990 3136 4282 585
Size: 2095 310 146 1146 775
Fragments arranged by size:
2095 1146 775 310 146
EcoICRI GAG'CTC
Cuts at: 1484 1484
Size: 4472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 672 732 2297 2729 3476 3744 513
Size: 159 60 1565 432 747 268 1241
Fragments arranged by size:
1565 1241 747 432 268 159 60
EcoNI CCTnn'n_nnAGG
Cuts at: 451 1311 451
Size: 860 3612
EcoO109I rG'GnC_Cy
Cuts at: 550 1055 1611 550
Size: 505 556 3411
Fragments arranged by size:
3411 556 505
EcoRI G'AATT_C
Cuts at: 1476 1476
Size: 4472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1350 2491 1350
Size: 1141 3331
FspI TGC'GCA
Cuts at: 1241 2253 1241
Size: 1012 3460
FspAI rTGC'GCAy
Cuts at: 1241 1241
Size: 4472
HaeII r_GCGC'y
Cuts at: 257 507 788 1227 1262 1278 1302 1906 2154
Size: 250 281 439 35 16 24 604 248
Cuts at: 2154 3176 4155 257
Size: 1022 979 574
Fragments arranged by size:
1022 979 604 574 439 281 250 248
35 24 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1351 1383 2314 2344 2346 2376 1351
Size: 32 931 30 2 30 3447
Fragments arranged by size:
3447 931 32 30 30 2
HincII GTy'rAC
Cuts at: 1511 1511
Size: 4472
KasI G'GCGC_C
Cuts at: 784 1298 1902 2150 784
Size: 514 604 248 3106
Fragments arranged by size:
3106 604 514 248
KpnI G_GTAC'C
Cuts at: 1492 1492
Size: 4472
MluI A'CGCG_T
Cuts at: 3905 3905
Size: 4472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3143 3327 3143
Size: 184 4288
MscI TGG'CCA
Cuts at: 184 2233 184
Size: 2049 2423
MslI CAynn'nnrTG
Cuts at: 161 229 274 349 364 370 2461 2588 161
Size: 68 45 75 15 6 2091 127 2045
Fragments arranged by size:
2091 2045 127 75 68 45 15 6
MspA1I CmG'CkG
Cuts at: 755 903 1583 1897 2257 3270 3515 755
Size: 148 680 314 360 1013 245 1712
Fragments arranged by size:
1712 1013 680 360 314 245 148
NaeI GCC'GGC
Cuts at: 2653 2653
Size: 4472
NarI GG'CG_CC
Cuts at: 785 1299 1903 2151 785
Size: 514 604 248 3106
Fragments arranged by size:
3106 604 514 248
NcoI C'CATG_G
Cuts at: 107 371 626 107
Size: 264 255 3953
Fragments arranged by size:
3953 264 255
NgoMIV G'CCGG_C
Cuts at: 2651 2651
Size: 4472
NspI r_CATG'y
Cuts at: 369 1240 1525 2025 2932 369
Size: 871 285 500 907 1909
Fragments arranged by size:
1909 907 871 500 285
PciI A'CATG_T
Cuts at: 2928 2928
Size: 4472
PflMI CCAn_nnn'nTGG
Cuts at: 755 755
Size: 4472
PmeI GTTT'AAAC
Cuts at: 1471 1471
Size: 4472
PmlI CAC'GTG
Cuts at: 483 483
Size: 4472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4440
PpuMI rG'GwC_Cy
Cuts at: 550 1055 550
Size: 505 3967
PstI C_TGCA'G
Cuts at: 284 463 1205 1440 1519 2204 284
Size: 179 742 235 79 685 2552
Fragments arranged by size:
2552 742 685 235 179 79
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4472
PvuII CAG'CTG
Cuts at: 903 1897 2257 903
Size: 994 360 3118
Fragments arranged by size:
3118 994 360
RsrII CG'GwC_CG
Cuts at: 2667 2667
Size: 4472
SacI G_AGCT'C
Cuts at: 1486 1486
Size: 4472
SalI G'TCGA_C
Cuts at: 1509 1509
Size: 4472
SapI GCTCTTCn'nnn_
Cuts at: 1291 2495 2705 1291
Size: 1204 210 3058
Fragments arranged by size:
3058 1204 210
SbfI CC_TGCA'GG
Cuts at: 1519 1519
Size: 4472
ScaI AGT'ACT
Cuts at: 3989 3989
Size: 4472
SfcI C'TryA_G
Cuts at: 33 280 381 459 1201 1436 1515 2200 3193
Size: 247 101 78 742 235 79 685 993
Cuts at: 3193 3384 33
Size: 191 1121
Fragments arranged by size:
1121 993 742 685 247 235 191 101
79 78
SfoI GGC'GCC
Cuts at: 786 1300 1904 2152 786
Size: 514 604 248 3106
Fragments arranged by size:
3106 604 514 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
SmaI CCC'GGG
Cuts at: 128 1494 128
Size: 1366 3106
SmlI C'TyrA_G
Cuts at: 1335 1390 1627 2542 3034 3296 1335
Size: 55 237 915 492 262 2511
Fragments arranged by size:
2511 915 492 262 237 55
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4472
SphI G_CATG'C
Cuts at: 369 1525 2025 369
Size: 1156 500 2816
Fragments arranged by size:
2816 1156 500
StuI AGG'CCT
Cuts at: 457 457
Size: 4472
StyI C'CwwG_G
Cuts at: 107 371 626 1606 1960 107
Size: 264 255 980 354 2619
Fragments arranged by size:
2619 980 354 264 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 132 286 1107 2055 2188 2647 2856 132
Size: 154 821 948 133 459 209 1748
Fragments arranged by size:
1748 948 821 459 209 154 133
TatI w'GTAC_w
Cuts at: 1136 3987 1136
Size: 2851 1621
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 107 139 879 911 3989 4021 107
Size: 32 740 32 3078 32 558
Fragments arranged by size:
3078 740 558 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 408 1053 2269 408
Size: 645 1216 2611
Fragments arranged by size:
2611 1216 645
XbaI T'CTAG_A
Cuts at: 1503 1503
Size: 4472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1967 1967
Size: 4472
XhoI C'TCGA_G
Cuts at: 1390 1390
Size: 4472
XmaI C'CCGG_G
Cuts at: 126 1492 126
Size: 1366 3106
XmnI GAAnn'nnTTC
Cuts at: 1330 1330
Size: 4472
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AlfI AloI ApaI AscI AvrII BaeI
BbsI BfrBI BmtI BsiWI BsmI BssHII BstXI BstZ17I
ClaI CspCI EcoRV FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI