Restriction map for
pF1KE0615
(Circular) (Six-base) MAPSORT of: pF1KE0615.seq Check: 2738 from: 1 to: 3635
pF1KE0615 3635 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AatII G_ACGT'C
Cuts at: 627 627
Size: 3635
AccI GT'mk_AC
Cuts at: 454 673 454
Size: 219 3416
Acc65I G'GTAC_C
Cuts at: 651 651
Size: 3635
AclI AA'CG_TT
Cuts at: 3475 3475
Size: 3635
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 1460 1892 2639 2907 466
Size: 994 432 747 268 1194
Fragments arranged by size:
1194 994 747 432 268
AflIII A'CryG_T
Cuts at: 987 2091 3068 987
Size: 1104 977 1554
Fragments arranged by size:
1554 1104 977
AgeI A'CCGG_T
Cuts at: 2023 2023
Size: 3635
AlwNI CAG_nnn'CTG
Cuts at: 940 2507 940
Size: 1567 2068
ApaLI G'TGCA_C
Cuts at: 2405 2405
Size: 3635
ApoI r'AATT_y
Cuts at: 382 639 2988 3244 382
Size: 257 2349 256 773
Fragments arranged by size:
2349 773 257 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3635
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3635
AvaI C'yCGr_G
Cuts at: 245 655 245
Size: 410 3225
BamHI G'GATC_C
Cuts at: 660 660
Size: 3635
BanI G'GyrC_C
Cuts at: 651 1065 1313 1348 651
Size: 414 248 35 2938
Fragments arranged by size:
2938 414 248 35
BanII G_rGCy'C
Cuts at: 649 1679 649
Size: 1030 2605
BbeI G_GCGC'C
Cuts at: 1069 1317 1069
Size: 248 3387
BbsI GAAGACnn'nnnn_
Cuts at: 374 603 374
Size: 229 3406
BciVI GTATCCnnnnn_n'
Cuts at: 1533 2294 3635 1533
Size: 761 1341 1533
Fragments arranged by size:
1533 1341 761
BclI T'GATC_A
Cuts at: 1155 1155
Size: 3635
BglII A'GATC_T
Cuts at: 123 1150 3187 123
Size: 1027 2037 571
Fragments arranged by size:
2037 1027 571
BlpI GC'TnA_GC
Cuts at: 747 747
Size: 3635
Bme1580I G_kGCm'C
Cuts at: 1260 1353 2409 1260
Size: 93 1056 2486
Fragments arranged by size:
2486 1056 93
BmgBI CAC'GTC
Cuts at: 117 117
Size: 3635
BmrI ACTGGGnnnn_n'
Cuts at: 847 928 1263 3407 847
Size: 81 335 2144 1075
Fragments arranged by size:
2144 1075 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2002 2002
Size: 3635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 362
Size: 3635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1690 2182 2480 811
Size: 879 492 298 1966
Fragments arranged by size:
1966 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3339 3339
Size: 3635
BsaAI yAC'GTr
Cuts at: 988 988
Size: 3635
BsaBI GATnn'nnATC
Cuts at: 6 1149 3186 6
Size: 1143 2037 455
Fragments arranged by size:
2037 1143 455
BsaHI Gr'CG_yC
Cuts at: 624 1066 1314 2016 3512 624
Size: 442 248 702 1496 747
Fragments arranged by size:
1496 747 702 442 248
BsaWI w'CCGG_w
Cuts at: 617 824 1345 2023 2297 2444 2855 617
Size: 207 521 678 274 147 411 1397
Fragments arranged by size:
1397 678 521 411 274 207 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 597 597
Size: 3635
BseYI C'CCAG_C
Cuts at: 136 1060 1818 2395 2891 136
Size: 924 758 577 496 880
Fragments arranged by size:
924 880 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1177 1223 2431 104
Size: 1073 46 1208 1308
Fragments arranged by size:
1308 1208 1073 46
BsiHKAI G_wGCw'C
Cuts at: 649 1427 1617 2409 649
Size: 778 190 792 1875
Fragments arranged by size:
1875 792 778 190
BsmI GAATG_Cn'
Cuts at: 179 179
Size: 3635
BsmBI CGTCTCn'nnnn_
Cuts at: 615 615
Size: 3635
Bsp1286I G_dGCh'C
Cuts at: 649 1260 1353 1427 1617 1679 2409 649
Size: 611 93 74 190 62 730 1875
Fragments arranged by size:
1875 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2855 2855
Size: 3635
BspHI T'CATG_A
Cuts at: 2811 2811
Size: 3635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 685 899 1201 1582 685
Size: 214 302 381 2738
Fragments arranged by size:
2738 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1927 1981 3465 3630 50
Size: 1877 54 1484 165 55
Fragments arranged by size:
1877 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1547 1547
Size: 3635
BsrFI r'CCGG_y
Cuts at: 1633 1814 2023 1633
Size: 181 209 3245
Fragments arranged by size:
3245 209 181
BssSI C'ACGA_G
Cuts at: 130 1906 2264 130
Size: 1776 358 1501
Fragments arranged by size:
1776 1501 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1012 1012
Size: 3635
BstBI TT'CG_AA
Cuts at: 1996 1996
Size: 3635
BstYI r'GATC_y
Cuts at: 1 123 660 1127 1150 1485 1731 2743 2829
Size: 122 537 467 23 335 246 1012 86
Cuts at: 2829 2841 3187 1
Size: 12 346 449
Fragments arranged by size:
1012 537 467 449 346 335 246 122
86 23 12
BstZ17I GTA'TAC
Cuts at: 455 455
Size: 3635
BtgI C'CryG_G
Cuts at: 107 155 107
Size: 48 3587
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 305 1585 1766 1929 305
Size: 1280 181 163 2011
Fragments arranged by size:
2011 1280 181 163
BtsI GCAGTG_nn'
Cuts at: 878 878
Size: 3635
DraI TTT'AAA
Cuts at: 634 634
Size: 3635
DrdI GACnn_nn'nnGTC
Cuts at: 1341 2199 1341
Size: 858 2777
EaeI y'GGCC_r
Cuts at: 513 591 1220 1394 1785 1812 3157 513
Size: 78 629 174 391 27 1345 991
Fragments arranged by size:
1345 991 629 391 174 78 27
EagI C'GGCC_G
Cuts at: 1220 1220
Size: 3635
EarI CTCTTCn'nnn_
Cuts at: 230 1658 1868 230
Size: 1428 210 1997
Fragments arranged by size:
1997 1428 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1843 2153 2299 3445 127
Size: 1716 310 146 1146 317
Fragments arranged by size:
1716 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 647 647
Size: 3635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 466 1460 1892 2639 2907 362
Size: 104 994 432 747 268 1090
Fragments arranged by size:
1090 994 747 432 268 104
EcoO109I rG'GnC_Cy
Cuts at: 242 774 242
Size: 532 3103
EcoRI G'AATT_C
Cuts at: 639 639
Size: 3635
EcoRV GAT'ATC
Cuts at: 442 442
Size: 3635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1654 1654
Size: 3635
FspI TGC'GCA
Cuts at: 1416 1416
Size: 3635
HaeII r_GCGC'y
Cuts at: 1069 1317 2339 3318 1069
Size: 248 1022 979 1386
Fragments arranged by size:
1386 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 521 553 1477 1507 1509 1539 521
Size: 32 924 30 2 30 2617
Fragments arranged by size:
2617 924 32 30 30 2
HincII GTy'rAC
Cuts at: 546 674 546
Size: 128 3507
KasI G'GCGC_C
Cuts at: 1065 1313 1065
Size: 248 3387
KpnI G_GTAC'C
Cuts at: 655 655
Size: 3635
MluI A'CGCG_T
Cuts at: 3068 3068
Size: 3635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2306 2490 2306
Size: 184 3451
MscI TGG'CCA
Cuts at: 515 1396 515
Size: 881 2754
MslI CAynn'nnrTG
Cuts at: 1624 1751 1624
Size: 127 3508
MspA1I CmG'CkG
Cuts at: 746 1060 1420 2433 2678 746
Size: 314 360 1013 245 1703
Fragments arranged by size:
1703 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1816 1816
Size: 3635
NarI GG'CG_CC
Cuts at: 1066 1314 1066
Size: 248 3387
NcoI C'CATG_G
Cuts at: 107 155 107
Size: 48 3587
NgoMIV G'CCGG_C
Cuts at: 1814 1814
Size: 3635
NspI r_CATG'y
Cuts at: 688 1188 2095 688
Size: 500 907 2228
Fragments arranged by size:
2228 907 500
PciI A'CATG_T
Cuts at: 2091 2091
Size: 3635
PmeI GTTT'AAAC
Cuts at: 634 634
Size: 3635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2774 2806 2774
Size: 32 3603
PpuMI rG'GwC_Cy
Cuts at: 242 242
Size: 3635
PstI C_TGCA'G
Cuts at: 682 1367 682
Size: 685 2950
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3635
PvuII CAG'CTG
Cuts at: 1060 1420 1060
Size: 360 3275
RsrII CG'GwC_CG
Cuts at: 1830 1830
Size: 3635
SacI G_AGCT'C
Cuts at: 649 649
Size: 3635
SalI G'TCGA_C
Cuts at: 672 672
Size: 3635
SanDI GG'GwC_CC
Cuts at: 242 242
Size: 3635
SapI GCTCTTCn'nnn_
Cuts at: 1658 1868 1658
Size: 210 3425
SbfI CC_TGCA'GG
Cuts at: 682 682
Size: 3635
ScaI AGT'ACT
Cuts at: 3152 3152
Size: 3635
SfcI C'TryA_G
Cuts at: 33 678 1363 2356 2547 33
Size: 645 685 993 191 1121
Fragments arranged by size:
1121 993 685 645 191
SfoI GGC'GCC
Cuts at: 1067 1315 1067
Size: 248 3387
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3635
SmaI CCC'GGG
Cuts at: 657 657
Size: 3635
SmlI C'TyrA_G
Cuts at: 790 1705 2197 2459 790
Size: 915 492 262 1966
Fragments arranged by size:
1966 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3635
SphI G_CATG'C
Cuts at: 688 1188 688
Size: 500 3135
StyI C'CwwG_G
Cuts at: 107 155 769 1123 107
Size: 48 614 354 2619
Fragments arranged by size:
2619 614 354 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1218 1351 1810 2019 1218
Size: 133 459 209 2834
Fragments arranged by size:
2834 459 209 133
TatI w'GTAC_w
Cuts at: 3150 3150
Size: 3635
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 3603
Tth111I GACn'n_nGTC
Cuts at: 1432 1432
Size: 3635
XbaI T'CTAG_A
Cuts at: 666 666
Size: 3635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1130 1130
Size: 3635
XmaI C'CCGG_G
Cuts at: 655 655
Size: 3635
ZraI GAC'GTC
Cuts at: 625 625
Size: 3635
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsaXI BsgI BsiWI BsrGI BssHII BstEII BstXI
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI