Restriction map for
pF1KE0617
(Circular) (Six-base) MAPSORT of: pF1KE0617.seq Check: 5206 from: 1 to: 3662
pF1KE0617 3662 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 549 700 549
Size: 151 3511
Acc65I G'GTAC_C
Cuts at: 678 678
Size: 3662
AclI AA'CG_TT
Cuts at: 3502 3502
Size: 3662
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 320 1487 1919 2666 2934 197
Size: 123 1167 432 747 268 925
Fragments arranged by size:
1167 925 747 432 268 123
AflIII A'CryG_T
Cuts at: 134 1014 2118 3095 134
Size: 880 1104 977 701
Fragments arranged by size:
1104 977 880 701
AgeI A'CCGG_T
Cuts at: 2050 2050
Size: 3662
AhdI GACnn_n'nnGTC
Cuts at: 171 171
Size: 3662
AlwNI CAG_nnn'CTG
Cuts at: 967 2534 967
Size: 1567 2095
ApaLI G'TGCA_C
Cuts at: 2432 2432
Size: 3662
ApoI r'AATT_y
Cuts at: 193 666 3015 3271 193
Size: 473 2349 256 584
Fragments arranged by size:
2349 584 473 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3662
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
AvaI C'yCGr_G
Cuts at: 682 682
Size: 3662
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 444 477 444
Size: 33 3629
BamHI G'GATC_C
Cuts at: 687 687
Size: 3662
BanI G'GyrC_C
Cuts at: 678 1092 1340 1375 678
Size: 414 248 35 2965
Fragments arranged by size:
2965 414 248 35
BanII G_rGCy'C
Cuts at: 676 1706 676
Size: 1030 2632
BbeI G_GCGC'C
Cuts at: 1096 1344 1096
Size: 248 3414
BbsI GAAGACnn'nnnn_
Cuts at: 185 308 567 185
Size: 123 259 3280
Fragments arranged by size:
3280 259 123
BciVI GTATCCnnnnn_n'
Cuts at: 1560 2321 3662 1560
Size: 761 1341 1560
Fragments arranged by size:
1560 1341 761
BclI T'GATC_A
Cuts at: 1182 1182
Size: 3662
BglII A'GATC_T
Cuts at: 1177 3214 1177
Size: 2037 1625
BlpI GC'TnA_GC
Cuts at: 774 774
Size: 3662
Bme1580I G_kGCm'C
Cuts at: 1287 1380 2436 1287
Size: 93 1056 2513
Fragments arranged by size:
2513 1056 93
BmgBI CAC'GTC
Cuts at: 137 137
Size: 3662
BmrI ACTGGGnnnn_n'
Cuts at: 615 874 955 1290 3434 615
Size: 259 81 335 2144 843
Fragments arranged by size:
2144 843 335 259 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 3662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1717 2209 2507 838
Size: 879 492 298 1993
Fragments arranged by size:
1993 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3366 3366
Size: 3662
BsaAI yAC'GTr
Cuts at: 1015 1015
Size: 3662
BsaBI GATnn'nnATC
Cuts at: 6 1176 3213 6
Size: 1170 2037 455
Fragments arranged by size:
2037 1170 455
BsaHI Gr'CG_yC
Cuts at: 1093 1341 2043 3539 1093
Size: 248 702 1496 1216
Fragments arranged by size:
1496 1216 702 248
BsaWI w'CCGG_w
Cuts at: 851 1372 2050 2324 2471 2882 851
Size: 521 678 274 147 411 1631
Fragments arranged by size:
1631 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 501 501
Size: 3662
BseYI C'CCAG_C
Cuts at: 1087 1845 2422 2918 1087
Size: 758 577 496 1831
Fragments arranged by size:
1831 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1204 1250 2458 104
Size: 1100 46 1208 1308
Fragments arranged by size:
1308 1208 1100 46
BsiHKAI G_wGCw'C
Cuts at: 676 1454 1644 2436 676
Size: 778 190 792 1902
Fragments arranged by size:
1902 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 143 143
Size: 3662
Bsp1286I G_dGCh'C
Cuts at: 676 1287 1380 1454 1644 1706 2436 676
Size: 611 93 74 190 62 730 1902
Fragments arranged by size:
1902 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2882 2882
Size: 3662
BspHI T'CATG_A
Cuts at: 2838 2838
Size: 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 128 712 926 1228 1609 128
Size: 584 214 302 381 2181
Fragments arranged by size:
2181 584 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1954 2008 3492 3657 50
Size: 1904 54 1484 165 55
Fragments arranged by size:
1904 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1574 1574
Size: 3662
BsrFI r'CCGG_y
Cuts at: 1660 1841 2050 1660
Size: 181 209 3272
Fragments arranged by size:
3272 209 181
BsrGI T'GTAC_A
Cuts at: 419 419
Size: 3662
BssSI C'ACGA_G
Cuts at: 1933 2291 1933
Size: 358 3304
BstAPI GCAn_nnn'nTGC
Cuts at: 1039 1039
Size: 3662
BstBI TT'CG_AA
Cuts at: 2023 2023
Size: 3662
BstYI r'GATC_y
Cuts at: 1 687 1154 1177 1512 1758 2770 2856 2868
Size: 686 467 23 335 246 1012 86 12
Cuts at: 2868 3214 1
Size: 346 449
Fragments arranged by size:
1012 686 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 565 565
Size: 3662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1612 1793 1956 1612
Size: 181 163 3318
Fragments arranged by size:
3318 181 163
BtsI GCAGTG_nn'
Cuts at: 905 905
Size: 3662
DraI TTT'AAA
Cuts at: 661 661
Size: 3662
DrdI GACnn_nn'nnGTC
Cuts at: 1368 2226 1368
Size: 858 2804
EaeI y'GGCC_r
Cuts at: 158 1247 1421 1812 1839 3184 158
Size: 1089 174 391 27 1345 636
Fragments arranged by size:
1345 1089 636 391 174 27
EagI C'GGCC_G
Cuts at: 1247 1247
Size: 3662
EarI CTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1870 2180 2326 3472 1870
Size: 310 146 1146 2060
Fragments arranged by size:
2060 1146 310 146
EcoICRI GAG'CTC
Cuts at: 674 674
Size: 3662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 320 1487 1919 2666 2934 197
Size: 123 1167 432 747 268 925
Fragments arranged by size:
1167 925 747 432 268 123
EcoO109I rG'GnC_Cy
Cuts at: 801 801
Size: 3662
EcoRI G'AATT_C
Cuts at: 666 666
Size: 3662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 521 1681 521
Size: 1160 2502
FspI TGC'GCA
Cuts at: 1443 1443
Size: 3662
HaeII r_GCGC'y
Cuts at: 1096 1344 2366 3345 1096
Size: 248 1022 979 1413
Fragments arranged by size:
1413 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 157 189 372 404 1504 1534 1536 1566 157
Size: 32 183 32 1100 30 2 30 2253
Fragments arranged by size:
2253 1100 183 32 32 30 30 2
HincII GTy'rAC
Cuts at: 701 701
Size: 3662
KasI G'GCGC_C
Cuts at: 1092 1340 1092
Size: 248 3414
KpnI G_GTAC'C
Cuts at: 682 682
Size: 3662
MluI A'CGCG_T
Cuts at: 3095 3095
Size: 3662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 611 2333 2517 611
Size: 1722 184 1756
Fragments arranged by size:
1756 1722 184
MscI TGG'CCA
Cuts at: 1423 1423
Size: 3662
MslI CAynn'nnrTG
Cuts at: 1651 1778 1651
Size: 127 3535
MspA1I CmG'CkG
Cuts at: 611 773 1087 1447 2460 2705 611
Size: 162 314 360 1013 245 1568
Fragments arranged by size:
1568 1013 360 314 245 162
NaeI GCC'GGC
Cuts at: 1843 1843
Size: 3662
NarI GG'CG_CC
Cuts at: 1093 1341 1093
Size: 248 3414
NgoMIV G'CCGG_C
Cuts at: 1841 1841
Size: 3662
NspI r_CATG'y
Cuts at: 715 1215 2122 715
Size: 500 907 2255
Fragments arranged by size:
2255 907 500
PasI CC'CwG_GG
Cuts at: 366 366
Size: 3662
PciI A'CATG_T
Cuts at: 2118 2118
Size: 3662
PmeI GTTT'AAAC
Cuts at: 661 661
Size: 3662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2801 2833 2801
Size: 32 3630
PstI C_TGCA'G
Cuts at: 610 709 1394 610
Size: 99 685 2878
Fragments arranged by size:
2878 685 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3662
PvuII CAG'CTG
Cuts at: 611 1087 1447 611
Size: 476 360 2826
Fragments arranged by size:
2826 476 360
RsrII CG'GwC_CG
Cuts at: 1857 1857
Size: 3662
SacI G_AGCT'C
Cuts at: 676 676
Size: 3662
SalI G'TCGA_C
Cuts at: 699 699
Size: 3662
SapI GCTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
SbfI CC_TGCA'GG
Cuts at: 709 709
Size: 3662
ScaI AGT'ACT
Cuts at: 3179 3179
Size: 3662
SexAI A'CCwGG_T
Cuts at: 169 169
Size: 3662
SfcI C'TryA_G
Cuts at: 33 212 519 606 705 1390 2383 2574 33
Size: 179 307 87 99 685 993 191 1121
Fragments arranged by size:
1121 993 685 307 191 179 99 87
SfoI GGC'GCC
Cuts at: 1094 1342 1094
Size: 248 3414
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
SmaI CCC'GGG
Cuts at: 684 684
Size: 3662
SmlI C'TyrA_G
Cuts at: 817 1732 2224 2486 817
Size: 915 492 262 1993
Fragments arranged by size:
1993 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3662
SphI G_CATG'C
Cuts at: 715 1215 715
Size: 500 3162
StyI C'CwwG_G
Cuts at: 401 796 1150 401
Size: 395 354 2913
Fragments arranged by size:
2913 395 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 491 567 1245 1378 1837 2046 491
Size: 76 678 133 459 209 2107
Fragments arranged by size:
2107 678 459 209 133 76
TatI w'GTAC_w
Cuts at: 419 623 3177 419
Size: 204 2554 904
Fragments arranged by size:
2554 904 204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 237 269 313 345 3179 3211 237
Size: 32 44 32 2834 32 688
Fragments arranged by size:
2834 688 44 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1459 1459
Size: 3662
XbaI T'CTAG_A
Cuts at: 463 693 463
Size: 230 3432
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 608 1157 608
Size: 549 3113
XmaI C'CCGG_G
Cuts at: 682 682
Size: 3662
XmnI GAAnn'nnTTC
Cuts at: 582 582
Size: 3662
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI