Restriction map for
pF1KE0618
(Circular) (Six-base) MAPSORT of: pF1KE0618.seq Check: 6167 from: 1 to: 4211
pF1KE0618 4211 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1249 1249
Size: 4211
Acc65I G'GTAC_C
Cuts at: 1227 1227
Size: 4211
AclI AA'CG_TT
Cuts at: 4051 4051
Size: 4211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2036 2468 3215 3483 2036
Size: 432 747 268 2764
Fragments arranged by size:
2764 747 432 268
AflIII A'CryG_T
Cuts at: 1563 2667 3644 1563
Size: 1104 977 2130
Fragments arranged by size:
2130 1104 977
AgeI A'CCGG_T
Cuts at: 2599 2599
Size: 4211
AhdI GACnn_n'nnGTC
Cuts at: 857 857
Size: 4211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1214 1217 1214
Size: 3 4208
AlwNI CAG_nnn'CTG
Cuts at: 375 783 990 1516 3083 375
Size: 408 207 526 1567 1503
Fragments arranged by size:
1567 1503 526 408 207
ApaI G_GGCC'C
Cuts at: 471 471
Size: 4211
ApaLI G'TGCA_C
Cuts at: 2981 2981
Size: 4211
ApoI r'AATT_y
Cuts at: 254 1215 3564 3820 254
Size: 961 2349 256 645
Fragments arranged by size:
2349 961 645 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4211
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
AvaI C'yCGr_G
Cuts at: 464 1231 464
Size: 767 3444
BamHI G'GATC_C
Cuts at: 1236 1236
Size: 4211
BanI G'GyrC_C
Cuts at: 1227 1641 1889 1924 1227
Size: 414 248 35 3514
Fragments arranged by size:
3514 414 248 35
BanII G_rGCy'C
Cuts at: 343 471 835 1225 2255 343
Size: 128 364 390 1030 2299
Fragments arranged by size:
2299 1030 390 364 128
BbeI G_GCGC'C
Cuts at: 1645 1893 1645
Size: 248 3963
BbsI GAAGACnn'nnnn_
Cuts at: 730 730
Size: 4211
BciVI GTATCCnnnnn_n'
Cuts at: 2109 2870 4211 2109
Size: 761 1341 2109
Fragments arranged by size:
2109 1341 761
BclI T'GATC_A
Cuts at: 1731 1731
Size: 4211
BglII A'GATC_T
Cuts at: 333 1726 3763 333
Size: 1393 2037 781
Fragments arranged by size:
2037 1393 781
BlpI GC'TnA_GC
Cuts at: 1323 1323
Size: 4211
Bme1580I G_kGCm'C
Cuts at: 471 1836 1929 2985 471
Size: 1365 93 1056 1697
Fragments arranged by size:
1697 1365 1056 93
BmgBI CAC'GTC
Cuts at: 750 750
Size: 4211
BmrI ACTGGGnnnn_n'
Cuts at: 1423 1504 1839 3983 1423
Size: 81 335 2144 1651
Fragments arranged by size:
2144 1651 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 4211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 847 1072 170
Size: 677 225 3309
Fragments arranged by size:
3309 677 225
Bpu10I CC'TnA_GC
Cuts at: 655 800 655
Size: 145 4066
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 2266 2758 3056 1387
Size: 879 492 298 2542
Fragments arranged by size:
2542 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 644 3915 644
Size: 3271 940
BsaAI yAC'GTr
Cuts at: 1564 1564
Size: 4211
BsaBI GATnn'nnATC
Cuts at: 6 1725 3762 6
Size: 1719 2037 455
Fragments arranged by size:
2037 1719 455
BsaHI Gr'CG_yC
Cuts at: 1642 1890 2592 4088 1642
Size: 248 702 1496 1765
Fragments arranged by size:
1765 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1400 1921 2599 2873 3020 3431 1400
Size: 521 678 274 147 411 2180
Fragments arranged by size:
2180 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 823 187
Size: 636 3575
BseYI C'CCAG_C
Cuts at: 1636 2394 2971 3467 1636
Size: 758 577 496 2380
Fragments arranged by size:
2380 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 1003 748
Size: 255 3956
BsiEI CG_ry'CG
Cuts at: 104 1753 1799 3007 104
Size: 1649 46 1208 1308
Fragments arranged by size:
1649 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1225 2003 2193 2985 1225
Size: 778 190 792 2451
Fragments arranged by size:
2451 792 778 190
BsmI GAATG_Cn'
Cuts at: 646 646
Size: 4211
Bsp1286I G_dGCh'C
Cuts at: 343 471 835 1225 1836 1929 2003 2193 2255
Size: 128 364 390 611 93 74 190 62
Cuts at: 2255 2985 343
Size: 730 1569
Fragments arranged by size:
1569 730 611 390 364 190 128 93
74 62
BspEI T'CCGG_A
Cuts at: 3431 3431
Size: 4211
BspHI T'CATG_A
Cuts at: 3387 3387
Size: 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1261 1475 1777 2158 1261
Size: 214 302 381 3314
Fragments arranged by size:
3314 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2503 2557 4041 4206 50
Size: 2453 54 1484 165 55
Fragments arranged by size:
2453 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2123 2123
Size: 4211
BsrFI r'CCGG_y
Cuts at: 2209 2390 2599 2209
Size: 181 209 3821
Fragments arranged by size:
3821 209 181
BssSI C'ACGA_G
Cuts at: 2482 2840 2482
Size: 358 3853
BstAPI GCAn_nnn'nTGC
Cuts at: 990 1588 990
Size: 598 3613
BstBI TT'CG_AA
Cuts at: 2572 2572
Size: 4211
BstYI r'GATC_y
Cuts at: 1 333 1236 1703 1726 2061 2307 3319 3405
Size: 332 903 467 23 335 246 1012 86
Cuts at: 3405 3417 3763 1
Size: 12 346 449
Fragments arranged by size:
1012 903 467 449 346 335 332 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 634 634
Size: 4211
BtgI C'CryG_G
Cuts at: 684 684
Size: 4211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2161 2342 2505 2161
Size: 181 163 3867
Fragments arranged by size:
3867 181 163
BtsI GCAGTG_nn'
Cuts at: 1454 1454
Size: 4211
DraI TTT'AAA
Cuts at: 1210 1210
Size: 4211
DrdI GACnn_nn'nnGTC
Cuts at: 1917 2775 1917
Size: 858 3353
EaeI y'GGCC_r
Cuts at: 968 1796 1970 2361 2388 3733 968
Size: 828 174 391 27 1345 1446
Fragments arranged by size:
1446 1345 828 391 174 27
EagI C'GGCC_G
Cuts at: 1796 1796
Size: 4211
EarI CTCTTCn'nnn_
Cuts at: 394 2234 2444 394
Size: 1840 210 2161
Fragments arranged by size:
2161 1840 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 820 2419 2729 2875 4021 820
Size: 1599 310 146 1146 1010
Fragments arranged by size:
1599 1146 1010 310 146
EcoICRI GAG'CTC
Cuts at: 1223 1223
Size: 4211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 847 1072 2036 2468 3215 3483 170
Size: 677 225 964 432 747 268 898
Fragments arranged by size:
964 898 747 677 432 268 225
EcoNI CCTnn'n_nnAGG
Cuts at: 268 799 900 268
Size: 531 101 3579
Fragments arranged by size:
3579 531 101
EcoO109I rG'GnC_Cy
Cuts at: 428 440 468 614 1350 428
Size: 12 28 146 736 3289
Fragments arranged by size:
3289 736 146 28 12
EcoRI G'AATT_C
Cuts at: 1215 1215
Size: 4211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 376 2230 376
Size: 1854 2357
FspI TGC'GCA
Cuts at: 994 1992 994
Size: 998 3213
HaeII r_GCGC'y
Cuts at: 1645 1893 2915 3894 1645
Size: 248 1022 979 1962
Fragments arranged by size:
1962 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 166 198 325 357 843 875 2053 2083 2085
Size: 32 127 32 486 32 1178 30 2
Cuts at: 2085 2115 166
Size: 30 2262
Fragments arranged by size:
2262 1178 486 127 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 552 1250 552
Size: 698 3513
KasI G'GCGC_C
Cuts at: 1641 1889 1641
Size: 248 3963
KpnI G_GTAC'C
Cuts at: 1231 1231
Size: 4211
MluI A'CGCG_T
Cuts at: 3644 3644
Size: 4211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 149 461 581 2882 3066 149
Size: 312 120 2301 184 1294
Fragments arranged by size:
2301 1294 312 184 120
MscI TGG'CCA
Cuts at: 970 1972 970
Size: 1002 3209
MslI CAynn'nnrTG
Cuts at: 841 979 2200 2327 841
Size: 138 1221 127 2725
Fragments arranged by size:
2725 1221 138 127
MspA1I CmG'CkG
Cuts at: 474 1322 1636 1996 3009 3254 474
Size: 848 314 360 1013 245 1431
Fragments arranged by size:
1431 1013 848 360 314 245
NaeI GCC'GGC
Cuts at: 2392 2392
Size: 4211
NarI GG'CG_CC
Cuts at: 1642 1890 1642
Size: 248 3963
NgoMIV G'CCGG_C
Cuts at: 2390 2390
Size: 4211
NspI r_CATG'y
Cuts at: 777 1264 1764 2671 777
Size: 487 500 907 2317
Fragments arranged by size:
2317 907 500 487
PciI A'CATG_T
Cuts at: 2667 2667
Size: 4211
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4211
PfoI T'CCnGG_A
Cuts at: 112 186 368 112
Size: 74 182 3955
Fragments arranged by size:
3955 182 74
PmeI GTTT'AAAC
Cuts at: 1210 1210
Size: 4211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 4179
PpuMI rG'GwC_Cy
Cuts at: 440 614 440
Size: 174 4037
PspOMI G'GGCC_C
Cuts at: 467 467
Size: 4211
PstI C_TGCA'G
Cuts at: 1193 1258 1943 1193
Size: 65 685 3461
Fragments arranged by size:
3461 685 65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4211
PvuII CAG'CTG
Cuts at: 1636 1996 1636
Size: 360 3851
RsrII CG'GwC_CG
Cuts at: 2406 2406
Size: 4211
SacI G_AGCT'C
Cuts at: 1225 1225
Size: 4211
SalI G'TCGA_C
Cuts at: 1248 1248
Size: 4211
SapI GCTCTTCn'nnn_
Cuts at: 2234 2444 2234
Size: 210 4001
SbfI CC_TGCA'GG
Cuts at: 1258 1258
Size: 4211
ScaI AGT'ACT
Cuts at: 3728 3728
Size: 4211
SfcI C'TryA_G
Cuts at: 33 1189 1254 1939 2932 3123 33
Size: 1156 65 685 993 191 1121
Fragments arranged by size:
1156 1121 993 685 191 65
SfoI GGC'GCC
Cuts at: 1643 1891 1643
Size: 248 3963
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
SmaI CCC'GGG
Cuts at: 1233 1233
Size: 4211
SmlI C'TyrA_G
Cuts at: 1366 2281 2773 3035 1366
Size: 915 492 262 2542
Fragments arranged by size:
2542 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4211
SphI G_CATG'C
Cuts at: 1264 1764 1264
Size: 500 3711
StyI C'CwwG_G
Cuts at: 1178 1345 1699 1178
Size: 167 354 3690
Fragments arranged by size:
3690 354 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 900 1794 1927 2386 2595 900
Size: 894 133 459 209 2516
Fragments arranged by size:
2516 894 459 209 133
TatI w'GTAC_w
Cuts at: 3726 3726
Size: 4211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 350 382 3728 3760 350
Size: 32 3346 32 801
Fragments arranged by size:
3346 801 32 32
Tth111I GACn'n_nGTC
Cuts at: 2008 2008
Size: 4211
XbaI T'CTAG_A
Cuts at: 1242 1242
Size: 4211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 376 682 1706 376
Size: 306 1024 2881
Fragments arranged by size:
2881 1024 306
XmaI C'CCGG_G
Cuts at: 1231 1231
Size: 4211
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI