Restriction map for
pF1KE0621
(Circular) (Six-base) MAPSORT of: pF1KE0621.seq Check: 1215 from: 1 to: 3683
pF1KE0621 3683 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 721 721
Size: 3683
Acc65I G'GTAC_C
Cuts at: 699 699
Size: 3683
AclI AA'CG_TT
Cuts at: 3523 3523
Size: 3683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 1940 2687 2955 1508
Size: 432 747 268 2236
Fragments arranged by size:
2236 747 432 268
AflIII A'CryG_T
Cuts at: 1035 2139 3116 1035
Size: 1104 977 1602
Fragments arranged by size:
1602 1104 977
AgeI A'CCGG_T
Cuts at: 2071 2071
Size: 3683
AlwNI CAG_nnn'CTG
Cuts at: 601 988 2555 601
Size: 387 1567 1729
Fragments arranged by size:
1729 1567 387
ApaLI G'TGCA_C
Cuts at: 2453 2453
Size: 3683
ApoI r'AATT_y
Cuts at: 687 3036 3292 687
Size: 2349 256 1078
Fragments arranged by size:
2349 1078 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3683
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
AvaI C'yCGr_G
Cuts at: 311 478 703 311
Size: 167 225 3291
Fragments arranged by size:
3291 225 167
BamHI G'GATC_C
Cuts at: 708 708
Size: 3683
BanI G'GyrC_C
Cuts at: 699 1113 1361 1396 699
Size: 414 248 35 2986
Fragments arranged by size:
2986 414 248 35
BanII G_rGCy'C
Cuts at: 166 697 1727 166
Size: 531 1030 2122
Fragments arranged by size:
2122 1030 531
BbeI G_GCGC'C
Cuts at: 1117 1365 1117
Size: 248 3435
BciVI GTATCCnnnnn_n'
Cuts at: 599 1581 2342 3683 599
Size: 982 761 1341 599
Fragments arranged by size:
1341 982 761 599
BclI T'GATC_A
Cuts at: 441 1203 441
Size: 762 2921
BfrBI ATG'CAT
Cuts at: 145 145
Size: 3683
BglII A'GATC_T
Cuts at: 1198 3235 1198
Size: 2037 1646
BlpI GC'TnA_GC
Cuts at: 795 795
Size: 3683
Bme1580I G_kGCm'C
Cuts at: 420 1308 1401 2457 420
Size: 888 93 1056 1646
Fragments arranged by size:
1646 1056 888 93
BmrI ACTGGGnnnn_n'
Cuts at: 895 976 1311 3455 895
Size: 81 335 2144 1123
Fragments arranged by size:
2144 1123 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 504 2050 504
Size: 1546 2137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 226 157
Size: 69 3614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 410 474 859 1738 2230 2528 217
Size: 193 64 385 879 492 298 1372
Fragments arranged by size:
1372 879 492 385 298 193 64
BsaI GGTCTCn'nnnn_
Cuts at: 3387 3387
Size: 3683
BsaAI yAC'GTr
Cuts at: 1036 1036
Size: 3683
BsaBI GATnn'nnATC
Cuts at: 6 1197 3234 6
Size: 1191 2037 455
Fragments arranged by size:
2037 1191 455
BsaHI Gr'CG_yC
Cuts at: 1114 1362 2064 3560 1114
Size: 248 702 1496 1237
Fragments arranged by size:
1496 1237 702 248
BsaWI w'CCGG_w
Cuts at: 872 1393 2071 2345 2492 2903 872
Size: 521 678 274 147 411 1652
Fragments arranged by size:
1652 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 229 220
Size: 9 3674
BseYI C'CCAG_C
Cuts at: 1108 1866 2443 2939 1108
Size: 758 577 496 1852
Fragments arranged by size:
1852 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1225 1271 2479 104
Size: 1121 46 1208 1308
Fragments arranged by size:
1308 1208 1121 46
BsiHKAI G_wGCw'C
Cuts at: 697 1475 1665 2457 697
Size: 778 190 792 1923
Fragments arranged by size:
1923 792 778 190
BsmI GAATG_Cn'
Cuts at: 147 147
Size: 3683
Bsp1286I G_dGCh'C
Cuts at: 166 420 697 1308 1401 1475 1665 1727 2457
Size: 254 277 611 93 74 190 62 730
Cuts at: 2457 166
Size: 1392
Fragments arranged by size:
1392 730 611 277 254 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2903 2903
Size: 3683
BspHI T'CATG_A
Cuts at: 2859 2859
Size: 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 733 947 1249 1630 733
Size: 214 302 381 2786
Fragments arranged by size:
2786 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1975 2029 3513 3678 50
Size: 1925 54 1484 165 55
Fragments arranged by size:
1925 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1595 1595
Size: 3683
BsrFI r'CCGG_y
Cuts at: 1681 1862 2071 1681
Size: 181 209 3293
Fragments arranged by size:
3293 209 181
BssSI C'ACGA_G
Cuts at: 1954 2312 1954
Size: 358 3325
BstAPI GCAn_nnn'nTGC
Cuts at: 1060 1060
Size: 3683
BstBI TT'CG_AA
Cuts at: 2044 2044
Size: 3683
BstXI CCAn_nnnn'nTGG
Cuts at: 250 402 250
Size: 152 3531
BstYI r'GATC_y
Cuts at: 1 708 1175 1198 1533 1779 2791 2877 2889
Size: 707 467 23 335 246 1012 86 12
Cuts at: 2889 3235 1
Size: 346 449
Fragments arranged by size:
1012 707 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3683
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 426 1633 1814 1977 426
Size: 1207 181 163 2132
Fragments arranged by size:
2132 1207 181 163
BtsI GCAGTG_nn'
Cuts at: 524 926 524
Size: 402 3281
DraI TTT'AAA
Cuts at: 682 682
Size: 3683
DrdI GACnn_nn'nnGTC
Cuts at: 1389 2247 1389
Size: 858 2825
EaeI y'GGCC_r
Cuts at: 1268 1442 1833 1860 3205 1268
Size: 174 391 27 1345 1746
Fragments arranged by size:
1746 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1268 1268
Size: 3683
EarI CTCTTCn'nnn_
Cuts at: 134 283 669 1706 1916 134
Size: 149 386 1037 210 1901
Fragments arranged by size:
1901 1037 386 210 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 583 1891 2201 2347 3493 583
Size: 1308 310 146 1146 773
Fragments arranged by size:
1308 1146 773 310 146
EcoICRI GAG'CTC
Cuts at: 695 695
Size: 3683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 226 1508 1940 2687 2955 157
Size: 69 1282 432 747 268 885
Fragments arranged by size:
1282 885 747 432 268 69
EcoNI CCTnn'n_nnAGG
Cuts at: 157 157
Size: 3683
EcoO109I rG'GnC_Cy
Cuts at: 822 822
Size: 3683
EcoRI G'AATT_C
Cuts at: 687 687
Size: 3683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1702 1702
Size: 3683
FspI TGC'GCA
Cuts at: 1464 1464
Size: 3683
HaeII r_GCGC'y
Cuts at: 601 1117 1365 2387 3366 601
Size: 516 248 1022 979 918
Fragments arranged by size:
1022 979 918 516 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 426 458 1525 1555 1557 1587 130
Size: 32 264 32 1067 30 2 30 2226
Fragments arranged by size:
2226 1067 264 32 32 30 30 2
HincII GTy'rAC
Cuts at: 330 722 330
Size: 392 3291
HpaI GTT'AAC
Cuts at: 330 330
Size: 3683
KasI G'GCGC_C
Cuts at: 1113 1361 1113
Size: 248 3435
KpnI G_GTAC'C
Cuts at: 703 703
Size: 3683
MluI A'CGCG_T
Cuts at: 3116 3116
Size: 3683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2354 2538 2354
Size: 184 3499
MscI TGG'CCA
Cuts at: 1444 1444
Size: 3683
MslI CAynn'nnrTG
Cuts at: 1672 1799 1672
Size: 127 3556
MspA1I CmG'CkG
Cuts at: 794 1108 1468 2481 2726 794
Size: 314 360 1013 245 1751
Fragments arranged by size:
1751 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1864 1864
Size: 3683
NarI GG'CG_CC
Cuts at: 1114 1362 1114
Size: 248 3435
NcoI C'CATG_G
Cuts at: 107 107
Size: 3683
NgoMIV G'CCGG_C
Cuts at: 1862 1862
Size: 3683
NsiI A_TGCA'T
Cuts at: 147 147
Size: 3683
NspI r_CATG'y
Cuts at: 424 736 1236 2143 424
Size: 312 500 907 1964
Fragments arranged by size:
1964 907 500 312
PciI A'CATG_T
Cuts at: 2139 2139
Size: 3683
PmeI GTTT'AAAC
Cuts at: 682 682
Size: 3683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2822 2854 2822
Size: 32 3651
PspXI vC'TCGA_Gb
Cuts at: 311 311
Size: 3683
PstI C_TGCA'G
Cuts at: 173 730 1415 173
Size: 557 685 2441
Fragments arranged by size:
2441 685 557
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3683
PvuII CAG'CTG
Cuts at: 1108 1468 1108
Size: 360 3323
RsrII CG'GwC_CG
Cuts at: 1878 1878
Size: 3683
SacI G_AGCT'C
Cuts at: 697 697
Size: 3683
SalI G'TCGA_C
Cuts at: 720 720
Size: 3683
SapI GCTCTTCn'nnn_
Cuts at: 1706 1916 1706
Size: 210 3473
SbfI CC_TGCA'GG
Cuts at: 730 730
Size: 3683
ScaI AGT'ACT
Cuts at: 3200 3200
Size: 3683
SfcI C'TryA_G
Cuts at: 33 169 726 1411 2404 2595 33
Size: 136 557 685 993 191 1121
Fragments arranged by size:
1121 993 685 557 191 136
SfoI GGC'GCC
Cuts at: 1115 1363 1115
Size: 248 3435
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3683
SmaI CCC'GGG
Cuts at: 705 705
Size: 3683
SmlI C'TyrA_G
Cuts at: 232 311 425 478 489 838 1753 2245 2507
Size: 79 114 53 11 349 915 492 262
Cuts at: 2507 232
Size: 1408
Fragments arranged by size:
1408 915 492 349 262 114 79 53
11
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3683
SphI G_CATG'C
Cuts at: 736 1236 736
Size: 500 3183
StyI C'CwwG_G
Cuts at: 107 817 1171 107
Size: 710 354 2619
Fragments arranged by size:
2619 710 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1266 1399 1858 2067 1266
Size: 133 459 209 2882
Fragments arranged by size:
2882 459 209 133
TatI w'GTAC_w
Cuts at: 3198 3198
Size: 3683
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 3651
Tth111I GACn'n_nGTC
Cuts at: 1480 1480
Size: 3683
XbaI T'CTAG_A
Cuts at: 652 714 652
Size: 62 3621
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 373 402 1178 373
Size: 29 776 2878
Fragments arranged by size:
2878 776 29
XhoI C'TCGA_G
Cuts at: 311 478 311
Size: 167 3516
XmaI C'CCGG_G
Cuts at: 703 703
Size: 3683
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII MfeI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI ZraI