Restriction map for
pF1KE0624
(Circular) (Six-base) MAPSORT of: pF1KE0624.seq Check: 1452 from: 1 to: 4223
pF1KE0624 4223 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 1261 1261
Size: 4223
Acc65I G'GTAC_C
Cuts at: 1239 1239
Size: 4223
AclI AA'CG_TT
Cuts at: 4063 4063
Size: 4223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 2048 2480 3227 3495 689
Size: 1359 432 747 268 1417
Fragments arranged by size:
1417 1359 747 432 268
AflIII A'CryG_T
Cuts at: 1575 2679 3656 1575
Size: 1104 977 2142
Fragments arranged by size:
2142 1104 977
AgeI A'CCGG_T
Cuts at: 2611 2611
Size: 4223
AleI CACnn'nnGTG
Cuts at: 1039 1039
Size: 4223
AlwNI CAG_nnn'CTG
Cuts at: 240 483 1132 1528 3095 240
Size: 243 649 396 1567 1368
Fragments arranged by size:
1567 1368 649 396 243
ApaLI G'TGCA_C
Cuts at: 2993 2993
Size: 4223
ApoI r'AATT_y
Cuts at: 1227 3576 3832 1227
Size: 2349 256 1618
Fragments arranged by size:
2349 1618 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4223
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
AvaI C'yCGr_G
Cuts at: 618 1243 618
Size: 625 3598
BamHI G'GATC_C
Cuts at: 1248 1248
Size: 4223
BanI G'GyrC_C
Cuts at: 1072 1239 1653 1901 1936 1072
Size: 167 414 248 35 3359
Fragments arranged by size:
3359 414 248 167 35
BanII G_rGCy'C
Cuts at: 416 1237 2267 416
Size: 821 1030 2372
Fragments arranged by size:
2372 1030 821
BbeI G_GCGC'C
Cuts at: 1657 1905 1657
Size: 248 3975
BbsI GAAGACnn'nnnn_
Cuts at: 575 575
Size: 4223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 757 791 1151 1185 90
Size: 34 633 34 360 34 3128
Fragments arranged by size:
3128 633 360 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 649 2121 2882 4223 649
Size: 1472 761 1341 649
Fragments arranged by size:
1472 1341 761 649
BclI T'GATC_A
Cuts at: 1743 1743
Size: 4223
BfrBI ATG'CAT
Cuts at: 257 257
Size: 4223
BglI GCCn_nnn'nGGC
Cuts at: 268 268
Size: 4223
BglII A'GATC_T
Cuts at: 1738 3775 1738
Size: 2037 2186
BlpI GC'TnA_GC
Cuts at: 1335 1335
Size: 4223
Bme1580I G_kGCm'C
Cuts at: 190 1075 1186 1848 1941 2997 190
Size: 885 111 662 93 1056 1416
Fragments arranged by size:
1416 1056 885 662 111 93
BmrI ACTGGGnnnn_n'
Cuts at: 840 1435 1516 1851 3995 840
Size: 595 81 335 2144 1068
Fragments arranged by size:
2144 1068 595 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 837 2590 837
Size: 1753 2470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 295
Size: 4223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 1399 2278 2770 3068 517
Size: 882 879 492 298 1672
Fragments arranged by size:
1672 882 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3927 3927
Size: 4223
BsaAI yAC'GTr
Cuts at: 660 1576 660
Size: 916 3307
BsaBI GATnn'nnATC
Cuts at: 6 1737 3774 6
Size: 1731 2037 455
Fragments arranged by size:
2037 1731 455
BsaHI Gr'CG_yC
Cuts at: 458 1654 1902 2604 4100 458
Size: 1196 248 702 1496 581
Fragments arranged by size:
1496 1196 702 581 248
BsaWI w'CCGG_w
Cuts at: 1412 1933 2611 2885 3032 3443 1412
Size: 521 678 274 147 411 2192
Fragments arranged by size:
2192 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 111 1068 1132 1648 2406 2983 3479 111
Size: 957 64 516 758 577 496 855
Fragments arranged by size:
957 855 758 577 516 496 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 4223
BsiEI CG_ry'CG
Cuts at: 104 1765 1811 3019 104
Size: 1661 46 1208 1308
Fragments arranged by size:
1661 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 416 632 1237 2015 2205 2997 416
Size: 216 605 778 190 792 1642
Fragments arranged by size:
1642 792 778 605 216 190
Bsp1286I G_dGCh'C
Cuts at: 190 416 632 1075 1186 1237 1848 1941 2015
Size: 226 216 443 111 51 611 93 74
Cuts at: 2015 2205 2267 2997 190
Size: 190 62 730 1416
Fragments arranged by size:
1416 730 611 443 226 216 190 111
93 74 62 51
BspEI T'CCGG_A
Cuts at: 3443 3443
Size: 4223
BspHI T'CATG_A
Cuts at: 581 3399 581
Size: 2818 1405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 509 1273 1487 1789 2170 509
Size: 764 214 302 381 2562
Fragments arranged by size:
2562 764 381 302 214
BsrBI CCG'CTC
Cuts at: 50 453 2515 2569 4053 4218 50
Size: 403 2062 54 1484 165 55
Fragments arranged by size:
2062 1484 403 165 55 54
BsrDI GCAATG_nn'
Cuts at: 257 2135 257
Size: 1878 2345
BsrFI r'CCGG_y
Cuts at: 1140 2221 2402 2611 1140
Size: 1081 181 209 2752
Fragments arranged by size:
2752 1081 209 181
BsrGI T'GTAC_A
Cuts at: 519 519
Size: 4223
BssSI C'ACGA_G
Cuts at: 2494 2852 2494
Size: 358 3865
BstAPI GCAn_nnn'nTGC
Cuts at: 918 1600 918
Size: 682 3541
BstBI TT'CG_AA
Cuts at: 2584 2584
Size: 4223
BstEII G'GTnAC_C
Cuts at: 748 748
Size: 4223
BstXI CCAn_nnnn'nTGG
Cuts at: 1041 1041
Size: 4223
BstYI r'GATC_y
Cuts at: 1 528 1248 1715 1738 2073 2319 3331 3417
Size: 527 720 467 23 335 246 1012 86
Cuts at: 3417 3429 3775 1
Size: 12 346 449
Fragments arranged by size:
1012 720 527 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 928 1034 1107 928
Size: 106 73 4044
Fragments arranged by size:
4044 106 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 711 2173 2354 2517 711
Size: 1462 181 163 2417
Fragments arranged by size:
2417 1462 181 163
BtsI GCAGTG_nn'
Cuts at: 125 630 1201 1466 125
Size: 505 571 265 2882
Fragments arranged by size:
2882 571 505 265
DraI TTT'AAA
Cuts at: 1222 1222
Size: 4223
DrdI GACnn_nn'nnGTC
Cuts at: 1929 2787 1929
Size: 858 3365
EaeI y'GGCC_r
Cuts at: 245 1808 1982 2373 2400 3745 245
Size: 1563 174 391 27 1345 723
Fragments arranged by size:
1563 1345 723 391 174 27
EagI C'GGCC_G
Cuts at: 1808 1808
Size: 4223
EarI CTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 160 2431 2741 2887 4033 160
Size: 2271 310 146 1146 350
Fragments arranged by size:
2271 1146 350 310 146
EcoICRI GAG'CTC
Cuts at: 414 1235 414
Size: 821 3402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 689 2048 2480 3227 3495 295
Size: 394 1359 432 747 268 1023
Fragments arranged by size:
1359 1023 747 432 394 268
EcoO109I rG'GnC_Cy
Cuts at: 127 194 270 615 885 1362 127
Size: 67 76 345 270 477 2988
Fragments arranged by size:
2988 477 345 270 76 67
EcoRI G'AATT_C
Cuts at: 1227 1227
Size: 4223
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1014 1041 2242 1014
Size: 27 1201 2995
Fragments arranged by size:
2995 1201 27
FspI TGC'GCA
Cuts at: 2004 2004
Size: 4223
HaeII r_GCGC'y
Cuts at: 1657 1905 2927 3906 1657
Size: 248 1022 979 1974
Fragments arranged by size:
1974 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 556 588 697 729 826 858 2065 2095 2097
Size: 32 109 32 97 32 1207 30 2
Cuts at: 2097 2127 556
Size: 30 2652
Fragments arranged by size:
2652 1207 109 97 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1262 1262
Size: 4223
KasI G'GCGC_C
Cuts at: 1653 1901 1653
Size: 248 3975
KpnI G_GTAC'C
Cuts at: 1243 1243
Size: 4223
MluI A'CGCG_T
Cuts at: 3656 3656
Size: 4223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 614 2894 3078 614
Size: 2280 184 1759
Fragments arranged by size:
2280 1759 184
MscI TGG'CCA
Cuts at: 247 1984 247
Size: 1737 2486
MslI CAynn'nnrTG
Cuts at: 1039 2212 2339 1039
Size: 1173 127 2923
Fragments arranged by size:
2923 1173 127
MspA1I CmG'CkG
Cuts at: 396 1068 1147 1334 1648 2008 3021 3266 396
Size: 672 79 187 314 360 1013 245 1353
Fragments arranged by size:
1353 1013 672 360 314 245 187 79
NaeI GCC'GGC
Cuts at: 1142 2404 1142
Size: 1262 2961
NarI GG'CG_CC
Cuts at: 1654 1902 1654
Size: 248 3975
NcoI C'CATG_G
Cuts at: 928 1107 928
Size: 179 4044
NgoMIV G'CCGG_C
Cuts at: 1140 2402 1140
Size: 1262 2961
NsiI A_TGCA'T
Cuts at: 259 259
Size: 4223
NspI r_CATG'y
Cuts at: 1276 1776 2683 1276
Size: 500 907 2816
Fragments arranged by size:
2816 907 500
PciI A'CATG_T
Cuts at: 2679 2679
Size: 4223
PflMI CCAn_nnn'nTGG
Cuts at: 483 1132 483
Size: 649 3574
PfoI T'CCnGG_A
Cuts at: 651 651
Size: 4223
PmeI GTTT'AAAC
Cuts at: 1222 1222
Size: 4223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 4191
PpuMI rG'GwC_Cy
Cuts at: 615 885 615
Size: 270 3953
PstI C_TGCA'G
Cuts at: 1067 1270 1955 1067
Size: 203 685 3335
Fragments arranged by size:
3335 685 203
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4223
PvuII CAG'CTG
Cuts at: 396 1068 1147 1648 2008 396
Size: 672 79 501 360 2611
Fragments arranged by size:
2611 672 501 360 79
RsrII CG'GwC_CG
Cuts at: 2418 2418
Size: 4223
SacI G_AGCT'C
Cuts at: 416 1237 416
Size: 821 3402
SalI G'TCGA_C
Cuts at: 1260 1260
Size: 4223
SapI GCTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
SbfI CC_TGCA'GG
Cuts at: 1270 1270
Size: 4223
ScaI AGT'ACT
Cuts at: 3740 3740
Size: 4223
SfcI C'TryA_G
Cuts at: 33 555 690 1063 1266 1951 2944 3135 33
Size: 522 135 373 203 685 993 191 1121
Fragments arranged by size:
1121 993 685 522 373 203 191 135
SfoI GGC'GCC
Cuts at: 1655 1903 1655
Size: 248 3975
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
SmaI CCC'GGG
Cuts at: 620 1245 620
Size: 625 3598
SmlI C'TyrA_G
Cuts at: 532 1378 2293 2785 3047 532
Size: 846 915 492 262 1708
Fragments arranged by size:
1708 915 846 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4223
SphI G_CATG'C
Cuts at: 1276 1776 1276
Size: 500 3723
StuI AGG'CCT
Cuts at: 916 916
Size: 4223
StyI C'CwwG_G
Cuts at: 263 928 1107 1357 1711 263
Size: 665 179 250 354 2775
Fragments arranged by size:
2775 665 354 250 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 941 1806 1939 2398 2607 941
Size: 865 133 459 209 2557
Fragments arranged by size:
2557 865 459 209 133
TatI w'GTAC_w
Cuts at: 519 551 3738 519
Size: 32 3187 1004
Fragments arranged by size:
3187 1004 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4191
Tth111I GACn'n_nGTC
Cuts at: 2020 2020
Size: 4223
XbaI T'CTAG_A
Cuts at: 951 1254 951
Size: 303 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 1718 270
Size: 1448 2775
XmaI C'CCGG_G
Cuts at: 618 1243 618
Size: 625 3598
XmnI GAAnn'nnTTC
Cuts at: 898 898
Size: 4223
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BmgBI BmtI Bpu10I BsaXI
BseRI BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI