Restriction map for
pF1KE0629
(Circular) (Six-base) MAPSORT of: pF1KE0629.seq Check: 8293 from: 1 to: 3707
pF1KE0629 3707 bp
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AccI GT'mk_AC
Cuts at: 745 745
Size: 3707
Acc65I G'GTAC_C
Cuts at: 723 723
Size: 3707
AclI AA'CG_TT
Cuts at: 3547 3547
Size: 3707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 1964 2711 2979 1532
Size: 432 747 268 2260
Fragments arranged by size:
2260 747 432 268
AflIII A'CryG_T
Cuts at: 578 1059 2163 3140 578
Size: 481 1104 977 1145
Fragments arranged by size:
1145 1104 977 481
AgeI A'CCGG_T
Cuts at: 631 2095 631
Size: 1464 2243
AlwNI CAG_nnn'CTG
Cuts at: 1012 2579 1012
Size: 1567 2140
ApaLI G'TGCA_C
Cuts at: 2477 2477
Size: 3707
ApoI r'AATT_y
Cuts at: 415 691 711 3060 3316 415
Size: 276 20 2349 256 806
Fragments arranged by size:
2349 806 276 256 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 3707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
AvaI C'yCGr_G
Cuts at: 727 727
Size: 3707
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 612 645 612
Size: 33 3674
BamHI G'GATC_C
Cuts at: 732 732
Size: 3707
BanI G'GyrC_C
Cuts at: 119 300 634 723 1137 1385 1420 119
Size: 181 334 89 414 248 35 2406
Fragments arranged by size:
2406 414 334 248 181 89 35
BanII G_rGCy'C
Cuts at: 131 236 721 1751 131
Size: 105 485 1030 2087
Fragments arranged by size:
2087 1030 485 105
BbeI G_GCGC'C
Cuts at: 1141 1389 1141
Size: 248 3459
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 207 241 207
Size: 34 3673
BciVI GTATCCnnnnn_n'
Cuts at: 1605 2366 3707 1605
Size: 761 1341 1605
Fragments arranged by size:
1605 1341 761
BclI T'GATC_A
Cuts at: 1227 1227
Size: 3707
BglII A'GATC_T
Cuts at: 1222 3259 1222
Size: 2037 1670
BlpI GC'TnA_GC
Cuts at: 819 819
Size: 3707
Bme1580I G_kGCm'C
Cuts at: 1332 1425 2481 1332
Size: 93 1056 2558
Fragments arranged by size:
2558 1056 93
BmgBI CAC'GTC
Cuts at: 581 581
Size: 3707
BmrI ACTGGGnnnn_n'
Cuts at: 264 919 1000 1335 3479 264
Size: 655 81 335 2144 492
Fragments arranged by size:
2144 655 492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 3707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 883 1762 2254 2552 145
Size: 738 879 492 298 1300
Fragments arranged by size:
1300 879 738 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3411 3411
Size: 3707
BsaAI yAC'GTr
Cuts at: 1060 1060
Size: 3707
BsaBI GATnn'nnATC
Cuts at: 6 1221 3258 6
Size: 1215 2037 455
Fragments arranged by size:
2037 1215 455
BsaHI Gr'CG_yC
Cuts at: 1138 1386 2088 3584 1138
Size: 248 702 1496 1261
Fragments arranged by size:
1496 1261 702 248
BsaWI w'CCGG_w
Cuts at: 631 896 1417 2095 2369 2516 2927 631
Size: 265 521 678 274 147 411 1411
Fragments arranged by size:
1411 678 521 411 274 265 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 202
Size: 3707
BseYI C'CCAG_C
Cuts at: 1132 1890 2467 2963 1132
Size: 758 577 496 1876
Fragments arranged by size:
1876 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1249 1295 2503 104
Size: 1145 46 1208 1308
Fragments arranged by size:
1308 1208 1145 46
BsiHKAI G_wGCw'C
Cuts at: 236 721 1499 1689 2481 236
Size: 485 778 190 792 1462
Fragments arranged by size:
1462 792 778 485 190
BsmI GAATG_Cn'
Cuts at: 336 336
Size: 3707
Bsp1286I G_dGCh'C
Cuts at: 131 236 721 1332 1425 1499 1689 1751 2481
Size: 105 485 611 93 74 190 62 730
Cuts at: 2481 131
Size: 1357
Fragments arranged by size:
1357 730 611 485 190 105 93 74
62
BspEI T'CCGG_A
Cuts at: 2927 2927
Size: 3707
BspHI T'CATG_A
Cuts at: 2883 2883
Size: 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 757 971 1273 1654 757
Size: 214 302 381 2810
Fragments arranged by size:
2810 381 302 214
BsrBI CCG'CTC
Cuts at: 50 573 1999 2053 3537 3702 50
Size: 523 1426 54 1484 165 55
Fragments arranged by size:
1484 1426 523 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1619 1619
Size: 3707
BsrFI r'CCGG_y
Cuts at: 631 1705 1886 2095 631
Size: 1074 181 209 2243
Fragments arranged by size:
2243 1074 209 181
BssSI C'ACGA_G
Cuts at: 1978 2336 1978
Size: 358 3349
BstAPI GCAn_nnn'nTGC
Cuts at: 1084 1084
Size: 3707
BstBI TT'CG_AA
Cuts at: 393 2068 393
Size: 1675 2032
BstYI r'GATC_y
Cuts at: 1 732 1199 1222 1557 1803 2815 2901 2913
Size: 731 467 23 335 246 1012 86 12
Cuts at: 2913 3259 1
Size: 346 449
Fragments arranged by size:
1012 731 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 173 173
Size: 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1657 1838 2001 1657
Size: 181 163 3363
Fragments arranged by size:
3363 181 163
BtsI GCAGTG_nn'
Cuts at: 950 950
Size: 3707
DraI TTT'AAA
Cuts at: 706 706
Size: 3707
DrdI GACnn_nn'nnGTC
Cuts at: 177 1413 2271 177
Size: 1236 858 1613
Fragments arranged by size:
1613 1236 858
EaeI y'GGCC_r
Cuts at: 1292 1466 1857 1884 3229 1292
Size: 174 391 27 1345 1770
Fragments arranged by size:
1770 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1292 1292
Size: 3707
EarI CTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 199 1915 2225 2371 3517 199
Size: 1716 310 146 1146 389
Fragments arranged by size:
1716 1146 389 310 146
EcoICRI GAG'CTC
Cuts at: 234 719 234
Size: 485 3222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 1964 2711 2979 1532
Size: 432 747 268 2260
Fragments arranged by size:
2260 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 222 846 222
Size: 624 3083
EcoRI G'AATT_C
Cuts at: 711 711
Size: 3707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1726 1726
Size: 3707
FspI TGC'GCA
Cuts at: 1488 1488
Size: 3707
HaeII r_GCGC'y
Cuts at: 1141 1389 2411 3390 1141
Size: 248 1022 979 1458
Fragments arranged by size:
1458 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 1549 1579 1581 1611 178
Size: 32 1339 30 2 30 2274
Fragments arranged by size:
2274 1339 32 30 30 2
HincII GTy'rAC
Cuts at: 746 746
Size: 3707
HindIII A'AGCT_T
Cuts at: 451 451
Size: 3707
KasI G'GCGC_C
Cuts at: 1137 1385 1137
Size: 248 3459
KpnI G_GTAC'C
Cuts at: 727 727
Size: 3707
MluI A'CGCG_T
Cuts at: 3140 3140
Size: 3707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2378 2562 2378
Size: 184 3523
MscI TGG'CCA
Cuts at: 1468 1468
Size: 3707
MslI CAynn'nnrTG
Cuts at: 1696 1823 1696
Size: 127 3580
MspA1I CmG'CkG
Cuts at: 175 376 818 1132 1492 2505 2750 175
Size: 201 442 314 360 1013 245 1132
Fragments arranged by size:
1132 1013 442 360 314 245 201
NaeI GCC'GGC
Cuts at: 1888 1888
Size: 3707
NarI GG'CG_CC
Cuts at: 1138 1386 1138
Size: 248 3459
NdeI CA'TA_TG
Cuts at: 436 436
Size: 3707
NgoMIV G'CCGG_C
Cuts at: 1886 1886
Size: 3707
NspI r_CATG'y
Cuts at: 760 1260 2167 760
Size: 500 907 2300
Fragments arranged by size:
2300 907 500
PciI A'CATG_T
Cuts at: 2163 2163
Size: 3707
PfoI T'CCnGG_A
Cuts at: 133 133
Size: 3707
PmeI GTTT'AAAC
Cuts at: 706 706
Size: 3707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 3675
PstI C_TGCA'G
Cuts at: 599 754 1439 599
Size: 155 685 2867
Fragments arranged by size:
2867 685 155
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3707
PvuII CAG'CTG
Cuts at: 376 1132 1492 376
Size: 756 360 2591
Fragments arranged by size:
2591 756 360
RsrII CG'GwC_CG
Cuts at: 1902 1902
Size: 3707
SacI G_AGCT'C
Cuts at: 236 721 236
Size: 485 3222
SacII CC_GC'GG
Cuts at: 176 176
Size: 3707
SalI G'TCGA_C
Cuts at: 744 744
Size: 3707
SapI GCTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
SbfI CC_TGCA'GG
Cuts at: 754 754
Size: 3707
ScaI AGT'ACT
Cuts at: 3224 3224
Size: 3707
SfcI C'TryA_G
Cuts at: 33 595 606 750 1435 2428 2619 33
Size: 562 11 144 685 993 191 1121
Fragments arranged by size:
1121 993 685 562 191 144 11
SfoI GGC'GCC
Cuts at: 1139 1387 1139
Size: 248 3459
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
SmaI CCC'GGG
Cuts at: 729 729
Size: 3707
SmlI C'TyrA_G
Cuts at: 124 862 1777 2269 2531 124
Size: 738 915 492 262 1300
Fragments arranged by size:
1300 915 738 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3707
SphI G_CATG'C
Cuts at: 760 1260 760
Size: 500 3207
StyI C'CwwG_G
Cuts at: 841 1195 841
Size: 354 3353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 226 1290 1423 1882 2091 226
Size: 1064 133 459 209 1842
Fragments arranged by size:
1842 1064 459 209 133
TatI w'GTAC_w
Cuts at: 3222 3222
Size: 3707
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 3675
Tth111I GACn'n_nGTC
Cuts at: 1504 1504
Size: 3707
XbaI T'CTAG_A
Cuts at: 738 738
Size: 3707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1202 1202
Size: 3707
XmaI C'CCGG_G
Cuts at: 727 727
Size: 3707
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BfrBI BglI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI