Restriction map for
pF1KE0638
(Circular) (Six-base) MAPSORT of: pF1KE0638.seq Check: 9382 from: 1 to: 4454
pF1KE0638 4454 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1274 1492 1274
Size: 218 4236
Acc65I G'GTAC_C
Cuts at: 1470 1470
Size: 4454
AclI AA'CG_TT
Cuts at: 4294 4294
Size: 4454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 2711 3458 3726 2279
Size: 432 747 268 3007
Fragments arranged by size:
3007 747 432 268
AflIII A'CryG_T
Cuts at: 1166 1806 2910 3887 1166
Size: 640 1104 977 1733
Fragments arranged by size:
1733 1104 977 640
AgeI A'CCGG_T
Cuts at: 2842 2842
Size: 4454
AleI CACnn'nnGTG
Cuts at: 597 688 597
Size: 91 4363
AlwNI CAG_nnn'CTG
Cuts at: 922 1759 3326 922
Size: 837 1567 2050
Fragments arranged by size:
2050 1567 837
ApaI G_GGCC'C
Cuts at: 1444 1444
Size: 4454
ApaLI G'TGCA_C
Cuts at: 1193 3224 1193
Size: 2031 2423
ApoI r'AATT_y
Cuts at: 1458 3807 4063 1458
Size: 2349 256 1849
Fragments arranged by size:
2349 1849 256
AscI GG'CGCG_CC
Cuts at: 1173 1173
Size: 4454
AseI AT'TA_AT
Cuts at: 20 20
Size: 4454
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
AvaI C'yCGr_G
Cuts at: 151 177 795 1188 1209 1474 151
Size: 26 618 393 21 265 3131
Fragments arranged by size:
3131 618 393 265 26 21
BamHI G'GATC_C
Cuts at: 1051 1479 1051
Size: 428 4026
BanI G'GyrC_C
Cuts at: 183 475 666 1470 1884 2132 2167 183
Size: 292 191 804 414 248 35 2470
Fragments arranged by size:
2470 804 414 292 248 191 35
BanII G_rGCy'C
Cuts at: 230 842 1353 1365 1444 1468 2498 230
Size: 612 511 12 79 24 1030 2186
Fragments arranged by size:
2186 1030 612 511 79 24 12
BbeI G_GCGC'C
Cuts at: 187 1888 2136 187
Size: 1701 248 2505
Fragments arranged by size:
2505 1701 248
BbsI GAAGACnn'nnnn_
Cuts at: 977 977
Size: 4454
BbvCI CC'TCA_GC
Cuts at: 217 217
Size: 4454
BciVI GTATCCnnnnn_n'
Cuts at: 2352 3113 4454 2352
Size: 761 1341 2352
Fragments arranged by size:
2352 1341 761
BclI T'GATC_A
Cuts at: 1974 1974
Size: 4454
BglII A'GATC_T
Cuts at: 1969 4006 1969
Size: 2037 2417
BlpI GC'TnA_GC
Cuts at: 535 1240 1414 1566 535
Size: 705 174 152 3423
Fragments arranged by size:
3423 705 174 152
Bme1580I G_kGCm'C
Cuts at: 463 671 1197 1444 2079 2172 3228 463
Size: 208 526 247 635 93 1056 1689
Fragments arranged by size:
1689 1056 635 526 247 208 93
BmrI ACTGGGnnnn_n'
Cuts at: 731 1447 1666 1747 2082 4226 731
Size: 716 219 81 335 2144 959
Fragments arranged by size:
2144 959 716 335 219 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 4454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 1039 577
Size: 462 3992
Bpu10I CC'TnA_GC
Cuts at: 217 217
Size: 4454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1630 2509 3001 3299 1630
Size: 879 492 298 2785
Fragments arranged by size:
2785 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4158 4158
Size: 4454
BsaAI yAC'GTr
Cuts at: 641 990 1169 1807 641
Size: 349 179 638 3288
Fragments arranged by size:
3288 638 349 179
BsaBI GATnn'nnATC
Cuts at: 6 1968 4005 6
Size: 1962 2037 455
Fragments arranged by size:
2037 1962 455
BsaHI Gr'CG_yC
Cuts at: 184 1885 2133 2835 4331 184
Size: 1701 248 702 1496 307
Fragments arranged by size:
1701 1496 702 307 248
BsaWI w'CCGG_w
Cuts at: 128 145 652 1643 2164 2842 3116 3263 3674
Size: 17 507 991 521 678 274 147 411
Cuts at: 3674 128
Size: 908
Fragments arranged by size:
991 908 678 521 507 411 274 147
17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 376 181
Size: 195 4259
BseYI C'CCAG_C
Cuts at: 1879 2637 3214 3710 1879
Size: 758 577 496 2623
Fragments arranged by size:
2623 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 157
Size: 4454
BsiEI CG_ry'CG
Cuts at: 104 1996 2042 3250 104
Size: 1892 46 1208 1308
Fragments arranged by size:
1892 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 842 1197 1206 1218 1353 1468 2246 2436 3228
Size: 355 9 12 135 115 778 190 792
Cuts at: 3228 842
Size: 2068
Fragments arranged by size:
2068 792 778 355 190 135 115 12
9
BsmI GAATG_Cn'
Cuts at: 236 236
Size: 4454
Bsp1286I G_dGCh'C
Cuts at: 230 463 671 842 1197 1206 1218 1353 1365
Size: 233 208 171 355 9 12 135 12
Cuts at: 1365 1444 1468 2079 2172 2246 2436 2498 3228
Size: 79 24 611 93 74 190 62 730
Cuts at: 3228 230
Size: 1456
Fragments arranged by size:
1456 730 611 355 233 208 190 171
135 93 79 74 62 24 12 12
9
BspEI T'CCGG_A
Cuts at: 652 3674 652
Size: 3022 1432
BspHI T'CATG_A
Cuts at: 3630 3630
Size: 4454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1504 1718 2020 2401 1504
Size: 214 302 381 3557
Fragments arranged by size:
3557 381 302 214
BsrBI CCG'CTC
Cuts at: 50 561 2746 2800 4284 4449 50
Size: 511 2185 54 1484 165 55
Fragments arranged by size:
2185 1484 511 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1394 2366 1394
Size: 972 3482
BsrFI r'CCGG_y
Cuts at: 944 2452 2633 2842 944
Size: 1508 181 209 2556
Fragments arranged by size:
2556 1508 209 181
BsrGI T'GTAC_A
Cuts at: 680 680
Size: 4454
BssHII G'CGCG_C
Cuts at: 1173 1173
Size: 4454
BssSI C'ACGA_G
Cuts at: 254 541 593 707 1346 2725 3083 254
Size: 287 52 114 639 1379 358 1625
Fragments arranged by size:
1625 1379 639 358 287 114 52
BstAPI GCAn_nnn'nTGC
Cuts at: 723 1831 723
Size: 1108 3346
BstBI TT'CG_AA
Cuts at: 2815 2815
Size: 4454
BstXI CCAn_nnnn'nTGG
Cuts at: 456 555 1083 456
Size: 99 528 3827
Fragments arranged by size:
3827 528 99
BstYI r'GATC_y
Cuts at: 1 1051 1479 1946 1969 2304 2550 3562 3648
Size: 1050 428 467 23 335 246 1012 86
Cuts at: 3648 3660 4006 1
Size: 12 346 449
Fragments arranged by size:
1050 1012 467 449 428 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 361 361
Size: 4454
BtgI C'CryG_G
Cuts at: 107 812 107
Size: 705 3749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 534 712 992 2404 2585 2748 534
Size: 178 280 1412 181 163 2240
Fragments arranged by size:
2240 1412 280 181 178 163
BtsI GCAGTG_nn'
Cuts at: 210 299 583 724 1697 210
Size: 89 284 141 973 2967
Fragments arranged by size:
2967 973 284 141 89
DraI TTT'AAA
Cuts at: 1453 1453
Size: 4454
DrdI GACnn_nn'nnGTC
Cuts at: 2160 3018 2160
Size: 858 3596
EaeI y'GGCC_r
Cuts at: 269 545 809 2039 2213 2604 2631 3976 269
Size: 276 264 1230 174 391 27 1345 747
Fragments arranged by size:
1345 1230 747 391 276 264 174 27
EagI C'GGCC_G
Cuts at: 2039 2039
Size: 4454
EarI CTCTTCn'nnn_
Cuts at: 1370 2477 2687 1370
Size: 1107 210 3137
Fragments arranged by size:
3137 1107 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 739 2662 2972 3118 4264 739
Size: 1923 310 146 1146 929
Fragments arranged by size:
1923 1146 929 310 146
EcoICRI GAG'CTC
Cuts at: 840 1351 1466 840
Size: 511 115 3828
Fragments arranged by size:
3828 511 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 1039 2279 2711 3458 3726 577
Size: 462 1240 432 747 268 1305
Fragments arranged by size:
1305 1240 747 462 432 268
EcoO109I rG'GnC_Cy
Cuts at: 703 1377 1441 1593 703
Size: 674 64 152 3564
Fragments arranged by size:
3564 674 152 64
EcoRI G'AATT_C
Cuts at: 1458 1458
Size: 4454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4454
FspI TGC'GCA
Cuts at: 2235 2235
Size: 4454
HaeII r_GCGC'y
Cuts at: 187 572 1033 1888 2136 3158 4137 187
Size: 385 461 855 248 1022 979 504
Fragments arranged by size:
1022 979 855 504 461 385 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2296 2326 2328 2358 2296
Size: 30 2 30 4392
Fragments arranged by size:
4392 30 30 2
HincII GTy'rAC
Cuts at: 1263 1493 1263
Size: 230 4224
KasI G'GCGC_C
Cuts at: 183 1884 2132 183
Size: 1701 248 2505
Fragments arranged by size:
2505 1701 248
KpnI G_GTAC'C
Cuts at: 1474 1474
Size: 4454
MluI A'CGCG_T
Cuts at: 3887 3887
Size: 4454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 294 3125 3309 294
Size: 2831 184 1439
Fragments arranged by size:
2831 1439 184
MscI TGG'CCA
Cuts at: 271 547 2215 271
Size: 276 1668 2510
Fragments arranged by size:
2510 1668 276
MslI CAynn'nnrTG
Cuts at: 334 511 597 688 2443 2570 334
Size: 177 86 91 1755 127 2218
Fragments arranged by size:
2218 1755 177 127 91 86
MspA1I CmG'CkG
Cuts at: 221 1565 1879 2239 3252 3497 221
Size: 1344 314 360 1013 245 1178
Fragments arranged by size:
1344 1178 1013 360 314 245
NaeI GCC'GGC
Cuts at: 946 2635 946
Size: 1689 2765
NarI GG'CG_CC
Cuts at: 184 1885 2133 184
Size: 1701 248 2505
Fragments arranged by size:
2505 1701 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4454
NgoMIV G'CCGG_C
Cuts at: 944 2633 944
Size: 1689 2765
NruI TCG'CGA
Cuts at: 836 836
Size: 4454
NspI r_CATG'y
Cuts at: 432 1507 2007 2914 432
Size: 1075 500 907 1972
Fragments arranged by size:
1972 1075 907 500
PasI CC'CwG_GG
Cuts at: 1358 1358
Size: 4454
PciI A'CATG_T
Cuts at: 2910 2910
Size: 4454
PflMI CCAn_nnn'nTGG
Cuts at: 1169 1169
Size: 4454
PmeI GTTT'AAAC
Cuts at: 1453 1453
Size: 4454
PmlI CAC'GTG
Cuts at: 641 1169 641
Size: 528 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4422
PpuMI rG'GwC_Cy
Cuts at: 1377 1377
Size: 4454
PspOMI G'GGCC_C
Cuts at: 1440 1440
Size: 4454
PstI C_TGCA'G
Cuts at: 590 719 1501 2186 590
Size: 129 782 685 2858
Fragments arranged by size:
2858 782 685 129
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4454
PvuII CAG'CTG
Cuts at: 221 1879 2239 221
Size: 1658 360 2436
Fragments arranged by size:
2436 1658 360
RsrII CG'GwC_CG
Cuts at: 2649 2649
Size: 4454
SacI G_AGCT'C
Cuts at: 842 1353 1468 842
Size: 511 115 3828
Fragments arranged by size:
3828 511 115
SalI G'TCGA_C
Cuts at: 1491 1491
Size: 4454
SapI GCTCTTCn'nnn_
Cuts at: 1370 2477 2687 1370
Size: 1107 210 3137
Fragments arranged by size:
3137 1107 210
SbfI CC_TGCA'GG
Cuts at: 1501 1501
Size: 4454
ScaI AGT'ACT
Cuts at: 3971 3971
Size: 4454
SexAI A'CCwGG_T
Cuts at: 757 757
Size: 4454
SfcI C'TryA_G
Cuts at: 33 586 715 1497 2182 3175 3366 33
Size: 553 129 782 685 993 191 1121
Fragments arranged by size:
1121 993 782 685 553 191 129
SfoI GGC'GCC
Cuts at: 185 1886 2134 185
Size: 1701 248 2505
Fragments arranged by size:
2505 1701 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
SmaI CCC'GGG
Cuts at: 153 179 797 1190 1476 153
Size: 26 618 393 286 3131
Fragments arranged by size:
3131 618 393 286 26
SmlI C'TyrA_G
Cuts at: 1609 2524 3016 3278 1609
Size: 915 492 262 2785
Fragments arranged by size:
2785 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4454
SphI G_CATG'C
Cuts at: 432 1507 2007 432
Size: 1075 500 2879
Fragments arranged by size:
2879 1075 500
StuI AGG'CCT
Cuts at: 1306 1306
Size: 4454
StyI C'CwwG_G
Cuts at: 107 903 1588 1942 107
Size: 796 685 354 2619
Fragments arranged by size:
2619 796 685 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1084 2037 2170 2629 2838 1084
Size: 953 133 459 209 2700
Fragments arranged by size:
2700 953 459 209 133
TatI w'GTAC_w
Cuts at: 680 3969 680
Size: 3289 1165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3971 4003 3971
Size: 32 4422
Tth111I GACn'n_nGTC
Cuts at: 759 2251 759
Size: 1492 2962
XbaI T'CTAG_A
Cuts at: 1485 1485
Size: 4454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1434 1949 1434
Size: 515 3939
XmaI C'CCGG_G
Cuts at: 151 177 795 1188 1474 151
Size: 26 618 393 286 3131
Fragments arranged by size:
3131 618 393 286 26
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PfoI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI