Restriction map for
pF1KE0641
(Circular) (Six-base) MAPSORT of: pF1KE0641.seq Check: 9483 from: 1 to: 3764
pF1KE0641 3764 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 802 802
Size: 3764
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 2021 2768 3036 1589
Size: 432 747 268 2317
Fragments arranged by size:
2317 747 432 268
AflII C'TTAA_G
Cuts at: 453 453
Size: 3764
AflIII A'CryG_T
Cuts at: 1116 2220 3197 1116
Size: 1104 977 1683
Fragments arranged by size:
1683 1104 977
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 654 686 654
Size: 32 3732
AlwNI CAG_nnn'CTG
Cuts at: 1069 2636 1069
Size: 1567 2197
ApaLI G'TGCA_C
Cuts at: 2534 2534
Size: 3764
ApoI r'AATT_y
Cuts at: 768 3117 3373 768
Size: 2349 256 1159
Fragments arranged by size:
2349 1159 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 784 784
Size: 3764
BamHI G'GATC_C
Cuts at: 588 789 588
Size: 201 3563
BanI G'GyrC_C
Cuts at: 780 1194 1442 1477 780
Size: 414 248 35 3067
Fragments arranged by size:
3067 414 248 35
BanII G_rGCy'C
Cuts at: 778 1808 778
Size: 1030 2734
BbeI G_GCGC'C
Cuts at: 1198 1446 1198
Size: 248 3516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 459 493 459
Size: 34 3730
BciVI GTATCCnnnnn_n'
Cuts at: 159 1662 2423 3764 159
Size: 1503 761 1341 159
Fragments arranged by size:
1503 1341 761 159
BclI T'GATC_A
Cuts at: 1284 1284
Size: 3764
BglII A'GATC_T
Cuts at: 1279 3316 1279
Size: 2037 1727
BlpI GC'TnA_GC
Cuts at: 876 876
Size: 3764
Bme1580I G_kGCm'C
Cuts at: 1389 1482 2538 1389
Size: 93 1056 2615
Fragments arranged by size:
2615 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 662 976 1057 1392 3536 662
Size: 314 81 335 2144 890
Fragments arranged by size:
2144 890 335 314 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1819 2311 2609 940
Size: 879 492 298 2095
Fragments arranged by size:
2095 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 1117 1117
Size: 3764
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 705 1195 1443 2145 3641 705
Size: 490 248 702 1496 828
Fragments arranged by size:
1496 828 702 490 248
BsaWI w'CCGG_w
Cuts at: 953 1474 2152 2426 2573 2984 953
Size: 521 678 274 147 411 1733
Fragments arranged by size:
1733 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1189 1947 2524 3020 1189
Size: 758 577 496 1933
Fragments arranged by size:
1933 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 281
Size: 3764
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2560 104
Size: 1202 46 1208 1308
Fragments arranged by size:
1308 1208 1202 46
BsiHKAI G_wGCw'C
Cuts at: 139 778 1556 1746 2538 139
Size: 639 778 190 792 1365
Fragments arranged by size:
1365 792 778 639 190
BsmI GAATG_Cn'
Cuts at: 622 622
Size: 3764
Bsp1286I G_dGCh'C
Cuts at: 139 778 1389 1482 1556 1746 1808 2538 139
Size: 639 611 93 74 190 62 730 1365
Fragments arranged by size:
1365 730 639 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 230 2940 230
Size: 2710 1054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 814 1028 1330 1711 814
Size: 214 302 381 2867
Fragments arranged by size:
2867 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2056 2110 3594 3759 50
Size: 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 359 1676 359
Size: 1317 2447
BsrFI r'CCGG_y
Cuts at: 1762 1943 2152 1762
Size: 181 209 3374
Fragments arranged by size:
3374 209 181
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 361 1141 361
Size: 780 2984
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstEII G'GTnAC_C
Cuts at: 222 222
Size: 3764
BstYI r'GATC_y
Cuts at: 1 588 789 1256 1279 1614 1860 2872 2958
Size: 587 201 467 23 335 246 1012 86
Cuts at: 2958 2970 3316 1
Size: 12 346 449
Fragments arranged by size:
1012 587 467 449 346 335 246 201
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1714 1895 2058 1714
Size: 181 163 3420
Fragments arranged by size:
3420 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1007
Size: 3764
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2328 1470
Size: 858 2906
EaeI y'GGCC_r
Cuts at: 1349 1523 1914 1941 3286 1349
Size: 174 391 27 1345 1827
Fragments arranged by size:
1827 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 247 372 1787 1997 247
Size: 125 1415 210 2014
Fragments arranged by size:
2014 1415 210 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1972 2282 2428 3574 1972
Size: 310 146 1146 2162
Fragments arranged by size:
2162 1146 310 146
EcoICRI GAG'CTC
Cuts at: 776 776
Size: 3764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 2021 2768 3036 1589
Size: 432 747 268 2317
Fragments arranged by size:
2317 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 610 610
Size: 3764
EcoO109I rG'GnC_Cy
Cuts at: 903 903
Size: 3764
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3764
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 1198 1446 2468 3447 1198
Size: 248 1022 979 1515
Fragments arranged by size:
1515 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 277 309 1606 1636 1638 1668 277
Size: 32 1297 30 2 30 2373
Fragments arranged by size:
2373 1297 32 30 30 2
HincII GTy'rAC
Cuts at: 803 803
Size: 3764
KasI G'GCGC_C
Cuts at: 1194 1442 1194
Size: 248 3516
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 176 2435 2619 176
Size: 2259 184 1321
Fragments arranged by size:
2259 1321 184
MscI TGG'CCA
Cuts at: 1525 1525
Size: 3764
MslI CAynn'nnrTG
Cuts at: 1753 1880 1753
Size: 127 3637
MspA1I CmG'CkG
Cuts at: 385 875 1189 1549 2562 2807 385
Size: 490 314 360 1013 245 1342
Fragments arranged by size:
1342 1013 490 360 314 245
NaeI GCC'GGC
Cuts at: 1945 1945
Size: 3764
NarI GG'CG_CC
Cuts at: 1195 1443 1195
Size: 248 3516
NgoMIV G'CCGG_C
Cuts at: 1943 1943
Size: 3764
NspI r_CATG'y
Cuts at: 817 1317 2224 817
Size: 500 907 2357
Fragments arranged by size:
2357 907 500
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 3732
PstI C_TGCA'G
Cuts at: 811 1496 811
Size: 685 3079
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 385 1189 1549 385
Size: 804 360 2600
Fragments arranged by size:
2600 804 360
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 778 778
Size: 3764
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 372 1787 1997 372
Size: 1415 210 2139
Fragments arranged by size:
2139 1415 210
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 3281 3281
Size: 3764
SfcI C'TryA_G
Cuts at: 33 807 1492 2485 2676 33
Size: 774 685 993 191 1121
Fragments arranged by size:
1121 993 774 685 191
SfoI GGC'GCC
Cuts at: 1196 1444 1196
Size: 248 3516
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 786 786
Size: 3764
SmlI C'TyrA_G
Cuts at: 453 919 1834 2326 2588 453
Size: 466 915 492 262 1629
Fragments arranged by size:
1629 915 492 466 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StyI C'CwwG_G
Cuts at: 709 898 1252 709
Size: 189 354 3221
Fragments arranged by size:
3221 354 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1347 1480 1939 2148 1347
Size: 133 459 209 2963
Fragments arranged by size:
2963 459 209 133
TatI w'GTAC_w
Cuts at: 3279 3279
Size: 3764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 3732
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1259 1259
Size: 3764
XmaI C'CCGG_G
Cuts at: 784 784
Size: 3764
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI