Restriction map for
pF1KE0644
(Circular) (Six-base) MAPSORT of: pF1KE0644.seq Check: 1784 from: 1 to: 4406
pF1KE0644 4406 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 212 484 1444 212
Size: 272 960 3174
Fragments arranged by size:
3174 960 272
Acc65I G'GTAC_C
Cuts at: 1422 1422
Size: 4406
AclI AA'CG_TT
Cuts at: 4246 4246
Size: 4406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2231 2663 3410 3678 2231
Size: 432 747 268 2959
Fragments arranged by size:
2959 747 432 268
AflIII A'CryG_T
Cuts at: 1758 2862 3839 1758
Size: 1104 977 2325
Fragments arranged by size:
2325 1104 977
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AhdI GACnn_n'nnGTC
Cuts at: 208 208
Size: 4406
AleI CACnn'nnGTG
Cuts at: 262 645 262
Size: 383 4023
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 349 484 349
Size: 135 4271
AlwNI CAG_nnn'CTG
Cuts at: 1711 3278 1711
Size: 1567 2839
ApaLI G'TGCA_C
Cuts at: 733 3176 733
Size: 2443 1963
ApoI r'AATT_y
Cuts at: 1197 1377 1410 3759 4015 1197
Size: 180 33 2349 256 1588
Fragments arranged by size:
2349 1588 256 180 33
AseI AT'TA_AT
Cuts at: 20 20
Size: 4406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 1426 1426
Size: 4406
BamHI G'GATC_C
Cuts at: 1431 1431
Size: 4406
BanI G'GyrC_C
Cuts at: 468 536 966 1422 1836 2084 2119 468
Size: 68 430 456 414 248 35 2755
Fragments arranged by size:
2755 456 430 414 248 68 35
BanII G_rGCy'C
Cuts at: 1420 2450 1420
Size: 1030 3376
BbeI G_GCGC'C
Cuts at: 1840 2088 1840
Size: 248 4158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 158 90
Size: 34 34 4338
Fragments arranged by size:
4338 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2304 3065 4406 2304
Size: 761 1341 2304
Fragments arranged by size:
2304 1341 761
BclI T'GATC_A
Cuts at: 1926 1926
Size: 4406
BfrBI ATG'CAT
Cuts at: 1105 1105
Size: 4406
BglI GCCn_nnn'nGGC
Cuts at: 112 244 112
Size: 132 4274
BglII A'GATC_T
Cuts at: 743 1921 3958 743
Size: 1178 2037 1191
Fragments arranged by size:
2037 1191 1178
BlpI GC'TnA_GC
Cuts at: 1518 1518
Size: 4406
Bme1580I G_kGCm'C
Cuts at: 737 969 2031 2124 3180 737
Size: 232 1062 93 1056 1963
Fragments arranged by size:
1963 1062 1056 232 93
BmrI ACTGGGnnnn_n'
Cuts at: 1618 1699 2034 4178 1618
Size: 81 335 2144 1846
Fragments arranged by size:
2144 1846 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 156 2773 156
Size: 2617 1789
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1163 403
Size: 760 3646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 1582 2461 2953 3251 685
Size: 897 879 492 298 1840
Fragments arranged by size:
1840 897 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4110 4110
Size: 4406
BsaAI yAC'GTr
Cuts at: 312 654 1759 312
Size: 342 1105 2959
Fragments arranged by size:
2959 1105 342
BsaBI GATnn'nnATC
Cuts at: 6 1920 3957 6
Size: 1914 2037 455
Fragments arranged by size:
2037 1914 455
BsaHI Gr'CG_yC
Cuts at: 1837 2085 2787 4283 1837
Size: 248 702 1496 1960
Fragments arranged by size:
1960 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1595 2116 2794 3068 3215 3626 1595
Size: 521 678 274 147 411 2375
Fragments arranged by size:
2375 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1161 1191 1161
Size: 30 4376
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1336 1336
Size: 4406
BseYI C'CCAG_C
Cuts at: 441 962 1831 2589 3166 3662 441
Size: 521 869 758 577 496 1185
Fragments arranged by size:
1185 869 758 577 521 496
BsiEI CG_ry'CG
Cuts at: 104 205 1948 1994 3202 104
Size: 101 1743 46 1208 1308
Fragments arranged by size:
1743 1308 1208 101 46
BsiHKAI G_wGCw'C
Cuts at: 257 269 737 1420 2198 2388 3180 257
Size: 12 468 683 778 190 792 1483
Fragments arranged by size:
1483 792 778 683 468 190 12
BsmI GAATG_Cn'
Cuts at: 401 1276 401
Size: 875 3531
Bsp1286I G_dGCh'C
Cuts at: 257 269 737 969 1420 2031 2124 2198 2388
Size: 12 468 232 451 611 93 74 190
Cuts at: 2388 2450 3180 257
Size: 62 730 1483
Fragments arranged by size:
1483 730 611 468 451 232 190 93
74 62 12
BspEI T'CCGG_A
Cuts at: 3626 3626
Size: 4406
BspHI T'CATG_A
Cuts at: 3582 3582
Size: 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 389 526 1456 1670 1972 2353 389
Size: 137 930 214 302 381 2442
Fragments arranged by size:
2442 930 381 302 214 137
BsrBI CCG'CTC
Cuts at: 50 207 2698 2752 4236 4401 50
Size: 157 2491 54 1484 165 55
Fragments arranged by size:
2491 1484 165 157 55 54
BsrDI GCAATG_nn'
Cuts at: 300 521 2318 300
Size: 221 1797 2388
Fragments arranged by size:
2388 1797 221
BsrFI r'CCGG_y
Cuts at: 194 259 2404 2585 2794 194
Size: 65 2145 181 209 1806
Fragments arranged by size:
2145 1806 209 181 65
BsrGI T'GTAC_A
Cuts at: 806 806
Size: 4406
BssSI C'ACGA_G
Cuts at: 250 2677 3035 250
Size: 2427 358 1621
Fragments arranged by size:
2427 1621 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1783 1783
Size: 4406
BstBI TT'CG_AA
Cuts at: 867 2767 867
Size: 1900 2506
BstEII G'GTnAC_C
Cuts at: 315 315
Size: 4406
BstYI r'GATC_y
Cuts at: 1 743 1431 1898 1921 2256 2502 3514 3600
Size: 742 688 467 23 335 246 1012 86
Cuts at: 3600 3612 3958 1
Size: 12 346 449
Fragments arranged by size:
1012 742 688 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 168 2356 2537 2700 168
Size: 2188 181 163 1874
Fragments arranged by size:
2188 1874 181 163
BtsI GCAGTG_nn'
Cuts at: 1649 1649
Size: 4406
DraI TTT'AAA
Cuts at: 561 1405 561
Size: 844 3562
DrdI GACnn_nn'nnGTC
Cuts at: 2112 2970 2112
Size: 858 3548
EaeI y'GGCC_r
Cuts at: 1991 2165 2556 2583 3928 1991
Size: 174 391 27 1345 2469
Fragments arranged by size:
2469 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1991 1991
Size: 4406
EarI CTCTTCn'nnn_
Cuts at: 710 778 2429 2639 710
Size: 68 1651 210 2477
Fragments arranged by size:
2477 1651 210 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2614 2924 3070 4216 2614
Size: 310 146 1146 2804
Fragments arranged by size:
2804 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1418 1418
Size: 4406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1163 2231 2663 3410 3678 403
Size: 760 1068 432 747 268 1131
Fragments arranged by size:
1131 1068 760 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1545 1545
Size: 4406
EcoRI G'AATT_C
Cuts at: 1377 1410 1377
Size: 33 4373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4406
FspI TGC'GCA
Cuts at: 337 2187 337
Size: 1850 2556
FspAI rTGC'GCAy
Cuts at: 337 337
Size: 4406
HaeII r_GCGC'y
Cuts at: 118 286 373 1840 2088 3110 4089 118
Size: 168 87 1467 248 1022 979 435
Fragments arranged by size:
1467 1022 979 435 248 168 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 194 226 2248 2278 2280 2310 194
Size: 32 2022 30 2 30 2290
Fragments arranged by size:
2290 2022 32 30 30 2
HincII GTy'rAC
Cuts at: 1445 1445
Size: 4406
KasI G'GCGC_C
Cuts at: 1836 2084 1836
Size: 248 4158
KpnI G_GTAC'C
Cuts at: 1426 1426
Size: 4406
MluI A'CGCG_T
Cuts at: 3839 3839
Size: 4406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 455 1100 3077 3261 455
Size: 645 1977 184 1600
Fragments arranged by size:
1977 1600 645 184
MscI TGG'CCA
Cuts at: 2167 2167
Size: 4406
MslI CAynn'nnrTG
Cuts at: 262 526 645 928 2395 2522 262
Size: 264 119 283 1467 127 2146
Fragments arranged by size:
2146 1467 283 264 127 119
MspA1I CmG'CkG
Cuts at: 490 1517 1831 2191 3204 3449 490
Size: 1027 314 360 1013 245 1447
Fragments arranged by size:
1447 1027 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2587 2587
Size: 4406
NarI GG'CG_CC
Cuts at: 1837 2085 1837
Size: 248 4158
NdeI CA'TA_TG
Cuts at: 986 986
Size: 4406
NgoMIV G'CCGG_C
Cuts at: 2585 2585
Size: 4406
NsiI A_TGCA'T
Cuts at: 1107 1107
Size: 4406
NspI r_CATG'y
Cuts at: 1402 1459 1959 2866 1402
Size: 57 500 907 2942
Fragments arranged by size:
2942 907 500 57
PciI A'CATG_T
Cuts at: 2862 2862
Size: 4406
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PmlI CAC'GTG
Cuts at: 654 654
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 4374
PstI C_TGCA'G
Cuts at: 1146 1453 2138 1146
Size: 307 685 3414
Fragments arranged by size:
3414 685 307
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 1831 2191 1831
Size: 360 4046
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 1420 1420
Size: 4406
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 2429 2639 2429
Size: 210 4196
SbfI CC_TGCA'GG
Cuts at: 1453 1453
Size: 4406
ScaI AGT'ACT
Cuts at: 3923 3923
Size: 4406
SfcI C'TryA_G
Cuts at: 33 1142 1449 2134 3127 3318 33
Size: 1109 307 685 993 191 1121
Fragments arranged by size:
1121 1109 993 685 307 191
SfoI GGC'GCC
Cuts at: 1838 2086 1838
Size: 248 4158
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SgrAI Cr'CCGG_yG
Cuts at: 259 259
Size: 4406
SmaI CCC'GGG
Cuts at: 1428 1428
Size: 4406
SmlI C'TyrA_G
Cuts at: 664 1561 2476 2968 3230 664
Size: 897 915 492 262 1840
Fragments arranged by size:
1840 915 897 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4406
SphI G_CATG'C
Cuts at: 1459 1959 1459
Size: 500 3906
StyI C'CwwG_G
Cuts at: 365 1540 1894 365
Size: 1175 354 2877
Fragments arranged by size:
2877 1175 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 657 1989 2122 2581 2790 657
Size: 1332 133 459 209 2273
Fragments arranged by size:
2273 1332 459 209 133
TatI w'GTAC_w
Cuts at: 806 3921 806
Size: 3115 1291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3923 3955 3923
Size: 32 4374
Tth111I GACn'n_nGTC
Cuts at: 2203 2203
Size: 4406
XbaI T'CTAG_A
Cuts at: 1437 1437
Size: 4406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1901 1901
Size: 4406
XmaI C'CCGG_G
Cuts at: 1426 1426
Size: 4406
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NsiI NspI PciI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BbsI BbvCI BmgBI BmtI Bpu10I BsgI BsiWI
BsmBI BssHII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI HindIII HpaI MfeI NcoI
NheI NotI NruI PacI PasI PflMI PfoI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI