Restriction map for
pF1KE0645
(Circular) (Six-base) MAPSORT of: pF1KE0645.seq Check: 1788 from: 1 to: 3764
pF1KE0645 3764 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 503 802 503
Size: 299 3465
Acc65I G'GTAC_C
Cuts at: 780 780
Size: 3764
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 2021 2768 3036 1589
Size: 432 747 268 2317
Fragments arranged by size:
2317 747 432 268
AflIII A'CryG_T
Cuts at: 1116 2220 3197 1116
Size: 1104 977 1683
Fragments arranged by size:
1683 1104 977
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 463 495 463
Size: 32 3732
AlwNI CAG_nnn'CTG
Cuts at: 1069 2636 1069
Size: 1567 2197
ApaLI G'TGCA_C
Cuts at: 751 2534 751
Size: 1783 1981
ApoI r'AATT_y
Cuts at: 768 3117 3373 768
Size: 2349 256 1159
Fragments arranged by size:
2349 1159 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 291 450 784 291
Size: 159 334 3271
Fragments arranged by size:
3271 334 159
BamHI G'GATC_C
Cuts at: 789 789
Size: 3764
BanI G'GyrC_C
Cuts at: 780 1194 1442 1477 780
Size: 414 248 35 3067
Fragments arranged by size:
3067 414 248 35
BanII G_rGCy'C
Cuts at: 230 379 566 778 1808 230
Size: 149 187 212 1030 2186
Fragments arranged by size:
2186 1030 212 187 149
BbeI G_GCGC'C
Cuts at: 1198 1446 1198
Size: 248 3516
BciVI GTATCCnnnnn_n'
Cuts at: 1662 2423 3764 1662
Size: 761 1341 1662
Fragments arranged by size:
1662 1341 761
BclI T'GATC_A
Cuts at: 1284 1284
Size: 3764
BglII A'GATC_T
Cuts at: 542 1279 3316 542
Size: 737 2037 990
Fragments arranged by size:
2037 990 737
BlpI GC'TnA_GC
Cuts at: 637 876 637
Size: 239 3525
Bme1580I G_kGCm'C
Cuts at: 755 1389 1482 2538 755
Size: 634 93 1056 1981
Fragments arranged by size:
1981 1056 634 93
BmrI ACTGGGnnnn_n'
Cuts at: 597 976 1057 1392 3536 597
Size: 379 81 335 2144 825
Fragments arranged by size:
2144 825 379 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 196 2131 196
Size: 1935 1829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 691
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 556 940 1819 2311 2609 166
Size: 390 384 879 492 298 1321
Fragments arranged by size:
1321 879 492 390 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 407 3468 407
Size: 3061 703
BsaAI yAC'GTr
Cuts at: 741 1117 741
Size: 376 3388
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 1195 1443 2145 3641 1195
Size: 248 702 1496 1318
Fragments arranged by size:
1496 1318 702 248
BsaWI w'CCGG_w
Cuts at: 953 1474 2152 2426 2573 2984 953
Size: 521 678 274 147 411 1733
Fragments arranged by size:
1733 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 404 434 463 493 404
Size: 30 29 30 3675
Fragments arranged by size:
3675 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 487 574 487
Size: 87 3677
BseYI C'CCAG_C
Cuts at: 1189 1947 2524 3020 1189
Size: 758 577 496 1933
Fragments arranged by size:
1933 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 763 442
Size: 321 3443
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2560 104
Size: 1202 46 1208 1308
Fragments arranged by size:
1308 1208 1202 46
BsiHKAI G_wGCw'C
Cuts at: 230 379 755 778 1556 1746 2538 230
Size: 149 376 23 778 190 792 1456
Fragments arranged by size:
1456 792 778 376 190 149 23
Bsp1286I G_dGCh'C
Cuts at: 230 379 566 755 778 1389 1482 1556 1746
Size: 149 187 189 23 611 93 74 190
Cuts at: 1746 1808 2538 230
Size: 62 730 1456
Fragments arranged by size:
1456 730 611 190 189 187 149 93
74 62 23
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 2940 2940
Size: 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 814 1028 1330 1711 814
Size: 214 302 381 2867
Fragments arranged by size:
2867 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2056 2110 3594 3759 50
Size: 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1676 1676
Size: 3764
BsrFI r'CCGG_y
Cuts at: 701 1762 1943 2152 701
Size: 1061 181 209 2313
Fragments arranged by size:
2313 1061 209 181
BsrGI T'GTAC_A
Cuts at: 125 125
Size: 3764
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 750 1141 750
Size: 391 3373
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstYI r'GATC_y
Cuts at: 1 185 542 789 1256 1279 1614 1860 2872
Size: 184 357 247 467 23 335 246 1012
Cuts at: 2872 2958 2970 3316 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 357 346 335 247 246
184 86 23 12
BtgI C'CryG_G
Cuts at: 107 116 283 648 730 107
Size: 9 167 365 82 3141
Fragments arranged by size:
3141 365 167 82 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 510 1714 1895 2058 510
Size: 1204 181 163 2216
Fragments arranged by size:
2216 1204 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1007
Size: 3764
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 1470 2328 1470
Size: 858 2906
EaeI y'GGCC_r
Cuts at: 314 1349 1523 1914 1941 3286 314
Size: 1035 174 391 27 1345 792
Fragments arranged by size:
1345 1035 792 391 174 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 314 1787 1997 314
Size: 1473 210 2081
Fragments arranged by size:
2081 1473 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 634 1972 2282 2428 3574 634
Size: 1338 310 146 1146 824
Fragments arranged by size:
1338 1146 824 310 146
EcoICRI GAG'CTC
Cuts at: 228 377 776 228
Size: 149 399 3216
Fragments arranged by size:
3216 399 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1589 2021 2768 3036 691
Size: 898 432 747 268 1419
Fragments arranged by size:
1419 898 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 469 469
Size: 3764
EcoO109I rG'GnC_Cy
Cuts at: 584 624 666 903 584
Size: 40 42 237 3445
Fragments arranged by size:
3445 237 42 40
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1783 1783
Size: 3764
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 678 1198 1446 2468 3447 678
Size: 520 248 1022 979 995
Fragments arranged by size:
1022 995 979 520 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1606 1636 1638 1668 1606
Size: 30 2 30 3702
Fragments arranged by size:
3702 30 30 2
HincII GTy'rAC
Cuts at: 504 803 504
Size: 299 3465
KasI G'GCGC_C
Cuts at: 1194 1442 1194
Size: 248 3516
KpnI G_GTAC'C
Cuts at: 784 784
Size: 3764
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2619 2435
Size: 184 3580
MscI TGG'CCA
Cuts at: 1525 1525
Size: 3764
MslI CAynn'nnrTG
Cuts at: 1753 1880 1753
Size: 127 3637
MspA1I CmG'CkG
Cuts at: 312 875 1189 1549 2562 2807 312
Size: 563 314 360 1013 245 1269
Fragments arranged by size:
1269 1013 563 360 314 245
NaeI GCC'GGC
Cuts at: 1945 1945
Size: 3764
NarI GG'CG_CC
Cuts at: 1195 1443 1195
Size: 248 3516
NcoI C'CATG_G
Cuts at: 107 107
Size: 3764
NgoMIV G'CCGG_C
Cuts at: 1943 1943
Size: 3764
NspI r_CATG'y
Cuts at: 817 1317 2224 817
Size: 500 907 2357
Fragments arranged by size:
2357 907 500
PasI CC'CwG_GG
Cuts at: 382 382
Size: 3764
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PfoI T'CCnGG_A
Cuts at: 362 668 362
Size: 306 3458
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 463 495 2903 2935 463
Size: 32 2408 32 1292
Fragments arranged by size:
2408 1292 32 32
PpuMI rG'GwC_Cy
Cuts at: 624 666 624
Size: 42 3722
PstI C_TGCA'G
Cuts at: 811 1496 811
Size: 685 3079
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 312 1189 1549 312
Size: 877 360 2527
Fragments arranged by size:
2527 877 360
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 230 379 778 230
Size: 149 399 3216
Fragments arranged by size:
3216 399 149
SalI G'TCGA_C
Cuts at: 502 801 502
Size: 299 3465
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 811 811
Size: 3764
ScaI AGT'ACT
Cuts at: 460 3281 460
Size: 2821 943
SexAI A'CCwGG_T
Cuts at: 416 755 416
Size: 339 3425
SfcI C'TryA_G
Cuts at: 33 140 807 1492 2485 2676 33
Size: 107 667 685 993 191 1121
Fragments arranged by size:
1121 993 685 667 191 107
SfoI GGC'GCC
Cuts at: 1196 1444 1196
Size: 248 3516
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 786 786
Size: 3764
SmlI C'TyrA_G
Cuts at: 181 571 919 1834 2326 2588 181
Size: 390 348 915 492 262 1357
Fragments arranged by size:
1357 915 492 390 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StuI AGG'CCT
Cuts at: 448 448
Size: 3764
StyI C'CwwG_G
Cuts at: 107 898 1252 107
Size: 791 354 2619
Fragments arranged by size:
2619 791 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1347 1480 1939 2148 1347
Size: 133 459 209 2963
Fragments arranged by size:
2963 459 209 133
TatI w'GTAC_w
Cuts at: 125 458 3279 125
Size: 333 2821 610
Fragments arranged by size:
2821 610 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 170 202 404 436 3281 3313 170
Size: 32 202 32 2845 32 621
Fragments arranged by size:
2845 621 202 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 686 1259 114
Size: 572 573 2619
Fragments arranged by size:
2619 573 572
XmaI C'CCGG_G
Cuts at: 784 784
Size: 3764
XmnI GAAnn'nnTTC
Cuts at: 323 460 323
Size: 137 3627
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI