Restriction map for
pF1KE0646
(Circular) (Six-base) MAPSORT of: pF1KE0646.seq Check: 9641 from: 1 to: 4253
pF1KE0646 4253 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 612 612
Size: 4253
AccI GT'mk_AC
Cuts at: 1291 1291
Size: 4253
Acc65I G'GTAC_C
Cuts at: 1269 1269
Size: 4253
AclI AA'CG_TT
Cuts at: 4093 4093
Size: 4253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 1097 2078 2510 3257 3525 157
Size: 940 981 432 747 268 885
Fragments arranged by size:
981 940 885 747 432 268
AfeI AGC'GCT
Cuts at: 178 178
Size: 4253
AflIII A'CryG_T
Cuts at: 1605 2709 3686 1605
Size: 1104 977 2172
Fragments arranged by size:
2172 1104 977
AgeI A'CCGG_T
Cuts at: 2641 2641
Size: 4253
AlwNI CAG_nnn'CTG
Cuts at: 580 948 1207 1558 3125 580
Size: 368 259 351 1567 1708
Fragments arranged by size:
1708 1567 368 351 259
ApaLI G'TGCA_C
Cuts at: 3023 3023
Size: 4253
ApoI r'AATT_y
Cuts at: 781 1257 3606 3862 781
Size: 476 2349 256 1172
Fragments arranged by size:
2349 1172 476 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
AvaI C'yCGr_G
Cuts at: 805 1273 805
Size: 468 3785
BamHI G'GATC_C
Cuts at: 1002 1278 1002
Size: 276 3977
BanI G'GyrC_C
Cuts at: 1084 1242 1269 1683 1931 1966 1084
Size: 158 27 414 248 35 3371
Fragments arranged by size:
3371 414 248 158 35 27
BanII G_rGCy'C
Cuts at: 149 430 560 775 1171 1267 2297 149
Size: 281 130 215 396 96 1030 2105
Fragments arranged by size:
2105 1030 396 281 215 130 96
BbeI G_GCGC'C
Cuts at: 1088 1687 1935 1088
Size: 599 248 3406
Fragments arranged by size:
3406 599 248
BbsI GAAGACnn'nnnn_
Cuts at: 959 959
Size: 4253
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4219
BciVI GTATCCnnnnn_n'
Cuts at: 2151 2912 4253 2151
Size: 761 1341 2151
Fragments arranged by size:
2151 1341 761
BclI T'GATC_A
Cuts at: 1773 1773
Size: 4253
BglII A'GATC_T
Cuts at: 1768 3805 1768
Size: 2037 2216
BlpI GC'TnA_GC
Cuts at: 529 1365 529
Size: 836 3417
Bme1580I G_kGCm'C
Cuts at: 642 742 1245 1878 1971 3027 642
Size: 100 503 633 93 1056 1868
Fragments arranged by size:
1868 1056 633 503 100 93
BmrI ACTGGGnnnn_n'
Cuts at: 606 1465 1546 1881 4025 606
Size: 859 81 335 2144 834
Fragments arranged by size:
2144 859 834 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 444 2620 444
Size: 2176 2077
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 431 439 910 925 165
Size: 266 8 471 15 3493
Fragments arranged by size:
3493 471 266 15 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1429 2308 2800 3098 1429
Size: 879 492 298 2584
Fragments arranged by size:
2584 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3957 3957
Size: 4253
BsaAI yAC'GTr
Cuts at: 743 1606 743
Size: 863 3390
BsaBI GATnn'nnATC
Cuts at: 6 715 1767 3804 6
Size: 709 1052 2037 455
Fragments arranged by size:
2037 1052 709 455
BsaHI Gr'CG_yC
Cuts at: 1085 1684 1932 2634 4130 1085
Size: 599 248 702 1496 1208
Fragments arranged by size:
1496 1208 702 599 248
BsaWI w'CCGG_w
Cuts at: 522 1442 1963 2641 2915 3062 3473 522
Size: 920 521 678 274 147 411 1302
Fragments arranged by size:
1302 920 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 523 823 922 1084 1225 436
Size: 87 300 99 162 141 3464
Fragments arranged by size:
3464 300 162 141 99 87
BseYI C'CCAG_C
Cuts at: 214 301 321 568 664 747 832 1678 2436
Size: 87 20 247 96 83 85 846 758
Cuts at: 2436 3013 3509 214
Size: 577 496 958
Fragments arranged by size:
958 846 758 577 496 247 96 87
85 83 20
BsiEI CG_ry'CG
Cuts at: 104 1034 1795 1841 3049 104
Size: 930 761 46 1208 1308
Fragments arranged by size:
1308 1208 930 761 46
BsiHKAI G_wGCw'C
Cuts at: 530 560 914 1267 2045 2235 3027 530
Size: 30 354 353 778 190 792 1756
Fragments arranged by size:
1756 792 778 354 353 190 30
Bsp1286I G_dGCh'C
Cuts at: 149 430 530 560 642 742 775 914 1171
Size: 281 100 30 82 100 33 139 257
Cuts at: 1171 1245 1267 1878 1971 2045 2235 2297 3027
Size: 74 22 611 93 74 190 62 730
Cuts at: 3027 149
Size: 1375
Fragments arranged by size:
1375 730 611 281 257 190 139 100
100 93 82 74 74 62 33 30
22
BspEI T'CCGG_A
Cuts at: 3473 3473
Size: 4253
BspHI T'CATG_A
Cuts at: 3429 3429
Size: 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 612 1303 1517 1819 2200 612
Size: 691 214 302 381 2665
Fragments arranged by size:
2665 691 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1178 2545 2599 4083 4248 50
Size: 1128 1367 54 1484 165 55
Fragments arranged by size:
1484 1367 1128 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2165 2165
Size: 4253
BsrFI r'CCGG_y
Cuts at: 2251 2432 2641 2251
Size: 181 209 3863
Fragments arranged by size:
3863 209 181
BssSI C'ACGA_G
Cuts at: 641 2524 2882 641
Size: 1883 358 2012
Fragments arranged by size:
2012 1883 358
BstAPI GCAn_nnn'nTGC
Cuts at: 885 1630 885
Size: 745 3508
BstBI TT'CG_AA
Cuts at: 2614 2614
Size: 4253
BstYI r'GATC_y
Cuts at: 1 1002 1278 1745 1768 2103 2349 3361 3447
Size: 1001 276 467 23 335 246 1012 86
Cuts at: 3447 3459 3805 1
Size: 12 346 449
Fragments arranged by size:
1012 1001 467 449 346 335 276 246
86 23 12
BtgI C'CryG_G
Cuts at: 224 815 224
Size: 591 3662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1199 2203 2384 2547 1199
Size: 1004 181 163 2905
Fragments arranged by size:
2905 1004 181 163
BtsI GCAGTG_nn'
Cuts at: 347 558 1071 1496 347
Size: 211 513 425 3104
Fragments arranged by size:
3104 513 425 211
DraI TTT'AAA
Cuts at: 1252 1252
Size: 4253
DraIII CAC_nnn'GTG
Cuts at: 1119 1119
Size: 4253
DrdI GACnn_nn'nnGTC
Cuts at: 280 1959 2817 280
Size: 1679 858 1716
Fragments arranged by size:
1716 1679 858
EaeI y'GGCC_r
Cuts at: 633 1031 1235 1838 2012 2403 2430 3775 633
Size: 398 204 603 174 391 27 1345 1111
Fragments arranged by size:
1345 1111 603 398 391 204 174 27
EagI C'GGCC_G
Cuts at: 1031 1838 1031
Size: 807 3446
EarI CTCTTCn'nnn_
Cuts at: 301 395 1073 2276 2486 301
Size: 94 678 1203 210 2068
Fragments arranged by size:
2068 1203 678 210 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2461 2771 2917 4063 2461
Size: 310 146 1146 2651
Fragments arranged by size:
2651 1146 310 146
EcoICRI GAG'CTC
Cuts at: 558 1265 558
Size: 707 3546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 165 431 439 910 925 1097 2078 2510
Size: 8 266 8 471 15 172 981 432
Cuts at: 2510 3257 3525 157
Size: 747 268 885
Fragments arranged by size:
981 885 747 471 432 268 266 172
15 8 8
EcoO109I rG'GnC_Cy
Cuts at: 390 622 1392 390
Size: 232 770 3251
Fragments arranged by size:
3251 770 232
EcoRI G'AATT_C
Cuts at: 1257 1257
Size: 4253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 4253
FspI TGC'GCA
Cuts at: 2034 2034
Size: 4253
HaeII r_GCGC'y
Cuts at: 180 490 1088 1687 1935 2957 3936 180
Size: 310 598 599 248 1022 979 497
Fragments arranged by size:
1022 979 599 598 497 310 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 667 695 699 727 901 933 2095 2125 2127
Size: 28 4 28 174 32 1162 30 2
Cuts at: 2127 2157 667
Size: 30 2763
Fragments arranged by size:
2763 1162 174 32 30 30 28 28
4 2
HincII GTy'rAC
Cuts at: 1292 1292
Size: 4253
KasI G'GCGC_C
Cuts at: 1084 1683 1931 1084
Size: 599 248 3406
Fragments arranged by size:
3406 599 248
KpnI G_GTAC'C
Cuts at: 1273 1273
Size: 4253
MluI A'CGCG_T
Cuts at: 3686 3686
Size: 4253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1103 2924 3108 1103
Size: 1821 184 2248
Fragments arranged by size:
2248 1821 184
MscI TGG'CCA
Cuts at: 2014 2014
Size: 4253
MslI CAynn'nnrTG
Cuts at: 790 983 2242 2369 790
Size: 193 1259 127 2674
Fragments arranged by size:
2674 1259 193 127
MspA1I CmG'CkG
Cuts at: 1218 1364 1678 2038 3051 3296 1218
Size: 146 314 360 1013 245 2175
Fragments arranged by size:
2175 1013 360 314 245 146
NaeI GCC'GGC
Cuts at: 2434 2434
Size: 4253
NarI GG'CG_CC
Cuts at: 1085 1684 1932 1085
Size: 599 248 3406
Fragments arranged by size:
3406 599 248
NgoMIV G'CCGG_C
Cuts at: 2432 2432
Size: 4253
NspI r_CATG'y
Cuts at: 826 1306 1806 2713 826
Size: 480 500 907 2366
Fragments arranged by size:
2366 907 500 480
PciI A'CATG_T
Cuts at: 2709 2709
Size: 4253
PflMI CCAn_nnn'nTGG
Cuts at: 580 948 580
Size: 368 3885
PmeI GTTT'AAAC
Cuts at: 1252 1252
Size: 4253
PmlI CAC'GTG
Cuts at: 743 743
Size: 4253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 4221
PstI C_TGCA'G
Cuts at: 565 698 1300 1985 565
Size: 133 602 685 2833
Fragments arranged by size:
2833 685 602 133
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4253
PvuII CAG'CTG
Cuts at: 1218 1678 2038 1218
Size: 460 360 3433
Fragments arranged by size:
3433 460 360
RsrII CG'GwC_CG
Cuts at: 2448 2448
Size: 4253
SacI G_AGCT'C
Cuts at: 560 1267 560
Size: 707 3546
SalI G'TCGA_C
Cuts at: 1290 1290
Size: 4253
SapI GCTCTTCn'nnn_
Cuts at: 2276 2486 2276
Size: 210 4043
SbfI CC_TGCA'GG
Cuts at: 1300 1300
Size: 4253
ScaI AGT'ACT
Cuts at: 3770 3770
Size: 4253
SfcI C'TryA_G
Cuts at: 33 453 561 694 1296 1981 2974 3165 33
Size: 420 108 133 602 685 993 191 1121
Fragments arranged by size:
1121 993 685 602 420 191 133 108
SfoI GGC'GCC
Cuts at: 1086 1685 1933 1086
Size: 599 248 3406
Fragments arranged by size:
3406 599 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
SmaI CCC'GGG
Cuts at: 1275 1275
Size: 4253
SmlI C'TyrA_G
Cuts at: 1408 2323 2815 3077 1408
Size: 915 492 262 2584
Fragments arranged by size:
2584 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4253
SphI G_CATG'C
Cuts at: 826 1306 1806 826
Size: 480 500 3273
Fragments arranged by size:
3273 500 480
StuI AGG'CCT
Cuts at: 1226 1226
Size: 4253
StyI C'CwwG_G
Cuts at: 1130 1387 1741 1130
Size: 257 354 3642
Fragments arranged by size:
3642 354 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1836 1969 2428 2637 1836
Size: 133 459 209 3452
Fragments arranged by size:
3452 459 209 133
TatI w'GTAC_w
Cuts at: 614 3768 614
Size: 3154 1099
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3770 3802 3770
Size: 32 4221
Tth111I GACn'n_nGTC
Cuts at: 2050 2050
Size: 4253
XbaI T'CTAG_A
Cuts at: 1284 1284
Size: 4253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 222 1748 222
Size: 1526 2727
XmaI C'CCGG_G
Cuts at: 1273 1273
Size: 4253
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI