FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 Query: pF1KE0648, 380 aa 1>>>pF1KE0648 380 - 380 aa - 380 aa Library: human.CCDS.faa 18511270 residues in 32554 sequences Statistics: Expectation_n fit: rho(ln(x))= 7.0043+/-0.00119; mu= 8.3759+/- 0.070 mean_var=178.8347+/-39.870, 0's: 0 Z-trim(107.2): 176 B-trim: 0 in 0/51 Lambda= 0.095907 statistics sampled from 9258 (9461) to 9258 sequences Algorithm: FASTA (3.7 Nov 2010) [optimized] Parameters: BL50 matrix (15:-5), open/ext: -10/-2 ktup: 2, E-join: 1 (0.66), E-opt: 0.2 (0.291), width: 16 Scan time: 2.860 The best scores are: opt bits E(32554) CCDS33266.1 NCK2 gene_id:8440|Hs108|chr2 ( 380) 2557 366.6 2e-101 CCDS3092.1 NCK1 gene_id:4690|Hs108|chr3 ( 377) 1794 261.1 1.2e-69 CCDS54644.1 NCK1 gene_id:4690|Hs108|chr3 ( 313) 1377 203.3 2.5e-52 >>CCDS33266.1 NCK2 gene_id:8440|Hs108|chr2 (380 aa) initn: 2557 init1: 2557 opt: 2557 Z-score: 1934.3 bits: 366.6 E(32554): 2e-101 Smith-Waterman score: 2557; 100.0% identity (100.0% similar) in 380 aa overlap (1-380:1-380) 10 20 30 40 50 60 pF1KE0 MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKN 10 20 30 40 50 60 70 80 90 100 110 120 pF1KE0 SLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 SLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKF 70 80 90 100 110 120 130 140 150 160 170 180 pF1KE0 AYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 AYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFL 130 140 150 160 170 180 190 200 210 220 230 240 pF1KE0 SLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 SLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNA 190 200 210 220 230 240 250 260 270 280 290 300 pF1KE0 RGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 RGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALN 250 260 270 280 290 300 310 320 330 340 350 360 pF1KE0 ERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHYK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS33 ERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHYK 310 320 330 340 350 360 370 380 pF1KE0 KAPIFTSEHGEKLYLVRALQ :::::::::::::::::::: CCDS33 KAPIFTSEHGEKLYLVRALQ 370 380 >>CCDS3092.1 NCK1 gene_id:4690|Hs108|chr3 (377 aa) initn: 1773 init1: 731 opt: 1794 Z-score: 1363.8 bits: 261.1 E(32554): 1.2e-69 Smith-Waterman score: 1794; 69.4% identity (87.4% similar) in 382 aa overlap (1-379:1-376) 10 20 30 40 50 60 pF1KE0 MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKN :.:::.:.::.::.:::.::::::::::::::::::.::::::. :.::.:::::::::: CCDS30 MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKN 10 20 30 40 50 60 70 80 90 100 110 120 pF1KE0 SLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKF : .:.:.:::::::::.::..:: :. : : .:. :. . : .:.::::.::.::: CCDS30 SARKASIVKNLKDTLGIGKVKRKPSVPD-SASPADDS-FVDPG---ERLYDLNMPAYVKF 70 80 90 100 110 130 140 150 160 170 180 pF1KE0 AYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFL :.:::::::::.::..: :::::::::::::::::.:::::::: :: : .. . : CCDS30 NYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSL 120 130 140 150 160 170 190 200 210 220 230 240 pF1KE0 SLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNA : . .: ..: . ..::::::.:::::: ..::::::::..:.::::::::::::::.. CCDS30 SEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKI 180 190 200 210 220 230 250 260 270 280 290 pF1KE0 RGQVGLVPKNYVVVLSDGP---ALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAEC :.:::::::::.:....: .:.:. :: .: :: .:.::: ::::.::::::: CCDS30 NGMVGLVPKNYVTVMQNNPLTSGLEPS-PPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEM 240 250 260 270 280 290 300 310 320 330 340 350 pF1KE0 ALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVE :::::: :::::::::::::.::::::::.:::::::::: ..:::::::.: ::.:::: CCDS30 ALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVE 300 310 320 330 340 350 360 370 380 pF1KE0 HYKKAPIFTSEHGEKLYLVRALQ :::::::::::.:::::::. : CCDS30 HYKKAPIFTSEQGEKLYLVKHLS 360 370 >>CCDS54644.1 NCK1 gene_id:4690|Hs108|chr3 (313 aa) initn: 1396 init1: 731 opt: 1377 Z-score: 1053.0 bits: 203.3 E(32554): 2.5e-52 Smith-Waterman score: 1377; 66.0% identity (84.9% similar) in 312 aa overlap (71-379:7-312) 50 60 70 80 90 100 pF1KE0 VRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYP .:: ...::..:: :. : : .:. :. . CCDS54 MDWLNVFKDFFSIGKVKRKPSVPD-SASPADDS-FV 10 20 30 110 120 130 140 150 160 pF1KE0 ANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWF : .:.::::.::.::: :.:::::::::.::..: :::::::::::::::::.::: CCDS54 DPG---ERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWF 40 50 60 70 80 90 170 180 190 200 210 220 pF1KE0 PSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGE ::::: :: : .. . :: . .: ..: . ..::::::.:::::: ..::::::::. CCDS54 PSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGD 100 110 120 130 140 150 230 240 250 260 270 pF1KE0 TMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGP---ALHPAHAPQISYTGPSSS .:.::::::::::::::.. :.:::::::::.:....: .:.:. :: .: :: . CCDS54 VMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPS-PPQCDYIRPSLT 160 170 180 190 200 210 280 290 300 310 320 330 pF1KE0 GRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQL :.::: ::::.::::::: :::::: :::::::::::::.::::::::.:::::::::: CCDS54 GKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQL 220 230 240 250 260 270 340 350 360 370 380 pF1KE0 VDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ ..:::::::.: ::.:::::::::::::::.:::::::. : CCDS54 KETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS 280 290 300 310 380 residues in 1 query sequences 18511270 residues in 32554 library sequences Tcomplib [36.3.4 Apr, 2011] (8 proc) start: Sat Nov 5 04:15:54 2016 done: Sat Nov 5 04:15:54 2016 Total Scan time: 2.860 Total Display time: 0.000 Function used was FASTA [36.3.4 Apr, 2011]