Restriction map for
pF1KE0650
(Circular) (Six-base) MAPSORT of: pF1KE0650.seq Check: 7266 from: 1 to: 4259
pF1KE0650 4259 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 240 240
Size: 4259
AccI GT'mk_AC
Cuts at: 362 1139 1297 362
Size: 777 158 3324
Fragments arranged by size:
3324 777 158
Acc65I G'GTAC_C
Cuts at: 1275 1275
Size: 4259
AclI AA'CG_TT
Cuts at: 4099 4099
Size: 4259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1189 2084 2516 3263 3531 343
Size: 846 895 432 747 268 1071
Fragments arranged by size:
1071 895 846 747 432 268
AfeI AGC'GCT
Cuts at: 442 442
Size: 4259
AflII C'TTAA_G
Cuts at: 655 655
Size: 4259
AflIII A'CryG_T
Cuts at: 779 1611 2715 3692 779
Size: 832 1104 977 1346
Fragments arranged by size:
1346 1104 977 832
AgeI A'CCGG_T
Cuts at: 2647 2647
Size: 4259
AhdI GACnn_n'nnGTC
Cuts at: 783 783
Size: 4259
AlwNI CAG_nnn'CTG
Cuts at: 255 576 1564 3131 255
Size: 321 988 1567 1383
Fragments arranged by size:
1567 1383 988 321
ApaI G_GGCC'C
Cuts at: 142 142
Size: 4259
ApaLI G'TGCA_C
Cuts at: 3029 3029
Size: 4259
ApoI r'AATT_y
Cuts at: 1263 3612 3868 1263
Size: 2349 256 1654
Fragments arranged by size:
2349 1654 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
AvaI C'yCGr_G
Cuts at: 861 1061 1279 861
Size: 200 218 3841
Fragments arranged by size:
3841 218 200
BamHI G'GATC_C
Cuts at: 1284 1284
Size: 4259
BanI G'GyrC_C
Cuts at: 1275 1689 1937 1972 1275
Size: 414 248 35 3562
Fragments arranged by size:
3562 414 248 35
BanII G_rGCy'C
Cuts at: 142 209 1273 2303 142
Size: 67 1064 1030 2098
Fragments arranged by size:
2098 1064 1030 67
BbeI G_GCGC'C
Cuts at: 1693 1941 1693
Size: 248 4011
BbsI GAAGACnn'nnnn_
Cuts at: 970 1177 970
Size: 207 4052
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1017 1051 1017
Size: 34 4225
BciVI GTATCCnnnnn_n'
Cuts at: 2157 2918 4259 2157
Size: 761 1341 2157
Fragments arranged by size:
2157 1341 761
BclI T'GATC_A
Cuts at: 1779 1779
Size: 4259
BfrBI ATG'CAT
Cuts at: 467 467
Size: 4259
BglI GCCn_nnn'nGGC
Cuts at: 896 896
Size: 4259
BglII A'GATC_T
Cuts at: 1774 3811 1774
Size: 2037 2222
BlpI GC'TnA_GC
Cuts at: 1371 1371
Size: 4259
Bme1580I G_kGCm'C
Cuts at: 142 149 321 1884 1977 3033 142
Size: 7 172 1563 93 1056 1368
Fragments arranged by size:
1563 1368 1056 172 93 7
BmrI ACTGGGnnnn_n'
Cuts at: 1098 1471 1552 1887 4031 1098
Size: 373 81 335 2144 1326
Fragments arranged by size:
2144 1326 373 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 4259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 304
Size: 4259
Bpu10I CC'TnA_GC
Cuts at: 680 680
Size: 4259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1435 2314 2806 3104 1435
Size: 879 492 298 2590
Fragments arranged by size:
2590 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 679 3963 679
Size: 3284 975
BsaAI yAC'GTr
Cuts at: 1612 1612
Size: 4259
BsaBI GATnn'nnATC
Cuts at: 6 1773 3810 6
Size: 1767 2037 455
Fragments arranged by size:
2037 1767 455
BsaHI Gr'CG_yC
Cuts at: 1690 1938 2640 4136 1690
Size: 248 702 1496 1813
Fragments arranged by size:
1813 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1448 1969 2647 2921 3068 3479 1448
Size: 521 678 274 147 411 2228
Fragments arranged by size:
2228 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 439 888 1120 1180 1215 142
Size: 297 449 232 60 35 3186
Fragments arranged by size:
3186 449 297 232 60 35
BseYI C'CCAG_C
Cuts at: 255 1684 2442 3019 3515 255
Size: 1429 758 577 496 999
Fragments arranged by size:
1429 999 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 562
Size: 4259
BsiEI CG_ry'CG
Cuts at: 104 1801 1847 3055 104
Size: 1697 46 1208 1308
Fragments arranged by size:
1697 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 189 1273 2051 2241 3033 189
Size: 1084 778 190 792 1415
Fragments arranged by size:
1415 1084 792 778 190
BsmI GAATG_Cn'
Cuts at: 469 469
Size: 4259
Bsp1286I G_dGCh'C
Cuts at: 142 149 189 209 321 1273 1884 1977 2051
Size: 7 40 20 112 952 611 93 74
Cuts at: 2051 2241 2303 3033 142
Size: 190 62 730 1368
Fragments arranged by size:
1368 952 730 611 190 112 93 74
62 40 20 7
BspEI T'CCGG_A
Cuts at: 3479 3479
Size: 4259
BspHI T'CATG_A
Cuts at: 1001 3435 1001
Size: 2434 1825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 240 1309 1523 1825 2206 240
Size: 1069 214 302 381 2293
Fragments arranged by size:
2293 1069 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2551 2605 4089 4254 50
Size: 2501 54 1484 165 55
Fragments arranged by size:
2501 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 590 2171 590
Size: 1581 2678
BsrFI r'CCGG_y
Cuts at: 1184 2257 2438 2647 1184
Size: 1073 181 209 2796
Fragments arranged by size:
2796 1073 209 181
BsrGI T'GTAC_A
Cuts at: 803 803
Size: 4259
BssSI C'ACGA_G
Cuts at: 2530 2888 2530
Size: 358 3901
BstAPI GCAn_nnn'nTGC
Cuts at: 1636 1636
Size: 4259
BstBI TT'CG_AA
Cuts at: 2620 2620
Size: 4259
BstXI CCAn_nnnn'nTGG
Cuts at: 1122 1122
Size: 4259
BstYI r'GATC_y
Cuts at: 1 1284 1751 1774 2109 2355 3367 3453 3465
Size: 1283 467 23 335 246 1012 86 12
Cuts at: 3465 3811 1
Size: 346 449
Fragments arranged by size:
1283 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 740 740
Size: 4259
BtgI C'CryG_G
Cuts at: 107 432 107
Size: 325 3934
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2209 2390 2553 2209
Size: 181 163 3915
Fragments arranged by size:
3915 181 163
BtsI GCAGTG_nn'
Cuts at: 227 560 656 1502 227
Size: 333 96 846 2984
Fragments arranged by size:
2984 846 333 96
ClaI AT'CG_AT
Cuts at: 1027 1027
Size: 4259
DraI TTT'AAA
Cuts at: 1258 1258
Size: 4259
DrdI GACnn_nn'nnGTC
Cuts at: 1965 2823 1965
Size: 858 3401
EaeI y'GGCC_r
Cuts at: 1124 1844 2018 2409 2436 3781 1124
Size: 720 174 391 27 1345 1602
Fragments arranged by size:
1602 1345 720 391 174 27
EagI C'GGCC_G
Cuts at: 1844 1844
Size: 4259
EarI CTCTTCn'nnn_
Cuts at: 865 2282 2492 865
Size: 1417 210 2632
Fragments arranged by size:
2632 1417 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 182 940 2467 2777 2923 4069 182
Size: 758 1527 310 146 1146 372
Fragments arranged by size:
1527 1146 758 372 310 146
EcoICRI GAG'CTC
Cuts at: 1271 1271
Size: 4259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 343 1189 2084 2516 3263 3531 304
Size: 39 846 895 432 747 268 1032
Fragments arranged by size:
1032 895 846 747 432 268 39
EcoO109I rG'GnC_Cy
Cuts at: 138 139 744 1164 1398 138
Size: 1 605 420 234 2999
Fragments arranged by size:
2999 605 420 234 1
EcoRI G'AATT_C
Cuts at: 1263 1263
Size: 4259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 4259
FspI TGC'GCA
Cuts at: 2040 2040
Size: 4259
HaeII r_GCGC'y
Cuts at: 444 1693 1941 2963 3942 444
Size: 1249 248 1022 979 761
Fragments arranged by size:
1249 1022 979 761 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 769 801 978 1010 2101 2131 2133 2163 769
Size: 32 177 32 1091 30 2 30 2865
Fragments arranged by size:
2865 1091 177 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1298 1298
Size: 4259
KasI G'GCGC_C
Cuts at: 1689 1937 1689
Size: 248 4011
KpnI G_GTAC'C
Cuts at: 1279 1279
Size: 4259
MluI A'CGCG_T
Cuts at: 3692 3692
Size: 4259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 863 2930 3114 863
Size: 2067 184 2008
Fragments arranged by size:
2067 2008 184
MscI TGG'CCA
Cuts at: 1126 2020 1126
Size: 894 3365
MslI CAynn'nnrTG
Cuts at: 457 602 2248 2375 457
Size: 145 1646 127 2341
Fragments arranged by size:
2341 1646 145 127
MspA1I CmG'CkG
Cuts at: 945 1370 1684 2044 3057 3302 945
Size: 425 314 360 1013 245 1902
Fragments arranged by size:
1902 1013 425 360 314 245
NaeI GCC'GGC
Cuts at: 2440 2440
Size: 4259
NarI GG'CG_CC
Cuts at: 1690 1938 1690
Size: 248 4011
NcoI C'CATG_G
Cuts at: 107 432 107
Size: 325 3934
NgoMIV G'CCGG_C
Cuts at: 2438 2438
Size: 4259
NsiI A_TGCA'T
Cuts at: 469 469
Size: 4259
NspI r_CATG'y
Cuts at: 429 783 1312 1812 2719 429
Size: 354 529 500 907 1969
Fragments arranged by size:
1969 907 529 500 354
PasI CC'CwG_GG
Cuts at: 789 789
Size: 4259
PciI A'CATG_T
Cuts at: 779 2715 779
Size: 1936 2323
PflMI CCAn_nnn'nTGG
Cuts at: 1029 1029
Size: 4259
PfoI T'CCnGG_A
Cuts at: 407 407
Size: 4259
PmeI GTTT'AAAC
Cuts at: 1258 1258
Size: 4259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 4227
PpuMI rG'GwC_Cy
Cuts at: 744 1164 744
Size: 420 3839
PspOMI G'GGCC_C
Cuts at: 138 138
Size: 4259
PstI C_TGCA'G
Cuts at: 540 707 1073 1306 1991 540
Size: 167 366 233 685 2808
Fragments arranged by size:
2808 685 366 233 167
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4259
PvuII CAG'CTG
Cuts at: 945 1684 2044 945
Size: 739 360 3160
Fragments arranged by size:
3160 739 360
RsrII CG'GwC_CG
Cuts at: 2454 2454
Size: 4259
SacI G_AGCT'C
Cuts at: 1273 1273
Size: 4259
SalI G'TCGA_C
Cuts at: 1296 1296
Size: 4259
SapI GCTCTTCn'nnn_
Cuts at: 2282 2492 2282
Size: 210 4049
SbfI CC_TGCA'GG
Cuts at: 1306 1306
Size: 4259
ScaI AGT'ACT
Cuts at: 3776 3776
Size: 4259
SexAI A'CCwGG_T
Cuts at: 1148 1250 1148
Size: 102 4157
SfcI C'TryA_G
Cuts at: 33 536 703 1069 1302 1987 2980 3171 33
Size: 503 167 366 233 685 993 191 1121
Fragments arranged by size:
1121 993 685 503 366 233 191 167
SfoI GGC'GCC
Cuts at: 1691 1939 1691
Size: 248 4011
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
SmaI CCC'GGG
Cuts at: 863 1281 863
Size: 418 3841
SmlI C'TyrA_G
Cuts at: 655 1414 2329 2821 3083 655
Size: 759 915 492 262 1831
Fragments arranged by size:
1831 915 759 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4259
SphI G_CATG'C
Cuts at: 1312 1812 1312
Size: 500 3759
StyI C'CwwG_G
Cuts at: 107 344 432 1121 1393 1747 107
Size: 237 88 689 272 354 2619
Fragments arranged by size:
2619 689 354 272 237 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 241 253 706 781 1842 1975 2434 2643 241
Size: 12 453 75 1061 133 459 209 1857
Fragments arranged by size:
1857 1061 459 453 209 133 75 12
TatI w'GTAC_w
Cuts at: 803 3774 803
Size: 2971 1288
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4227
Tth111I GACn'n_nGTC
Cuts at: 1096 2056 1096
Size: 960 3299
XbaI T'CTAG_A
Cuts at: 1290 1290
Size: 4259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 252 301 1754 252
Size: 49 1453 2757
Fragments arranged by size:
2757 1453 49
XmaI C'CCGG_G
Cuts at: 861 1279 861
Size: 418 3841
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AleI AlfI AloI AscI AvrII BaeI BbvCI
BmgBI BmtI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI