Restriction map for
pF1KE0661
(Circular) (Six-base) MAPSORT of: pF1KE0661.seq Check: 9707 from: 1 to: 3821
pF1KE0661 3821 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 859 859
Size: 3821
Acc65I G'GTAC_C
Cuts at: 837 837
Size: 3821
AclI AA'CG_TT
Cuts at: 3661 3661
Size: 3821
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1646 2078 2825 3093 1646
Size: 432 747 268 2374
Fragments arranged by size:
2374 747 432 268
AflIII A'CryG_T
Cuts at: 513 1173 2277 3254 513
Size: 660 1104 977 1080
Fragments arranged by size:
1104 1080 977 660
AgeI A'CCGG_T
Cuts at: 2209 2209
Size: 3821
AlwNI CAG_nnn'CTG
Cuts at: 532 595 598 658 754 757 795 1126 2693
Size: 63 3 60 96 3 38 331 1567
Cuts at: 2693 532
Size: 1660
Fragments arranged by size:
1660 1567 331 96 63 60 38 3
3
ApaLI G'TGCA_C
Cuts at: 2591 2591
Size: 3821
ApoI r'AATT_y
Cuts at: 825 3174 3430 825
Size: 2349 256 1216
Fragments arranged by size:
2349 1216 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3821
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3821
AvaI C'yCGr_G
Cuts at: 260 841 260
Size: 581 3240
BamHI G'GATC_C
Cuts at: 318 846 318
Size: 528 3293
BanI G'GyrC_C
Cuts at: 720 837 1251 1499 1534 720
Size: 117 414 248 35 3007
Fragments arranged by size:
3007 414 248 117 35
BanII G_rGCy'C
Cuts at: 835 1865 835
Size: 1030 2791
BbeI G_GCGC'C
Cuts at: 1255 1503 1255
Size: 248 3573
BbsI GAAGACnn'nnnn_
Cuts at: 198 198
Size: 3821
BbvCI CC'TCA_GC
Cuts at: 788 788
Size: 3821
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 180 214 90
Size: 34 56 34 3697
Fragments arranged by size:
3697 56 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 449 1719 2480 3821 449
Size: 1270 761 1341 449
Fragments arranged by size:
1341 1270 761 449
BclI T'GATC_A
Cuts at: 1341 1341
Size: 3821
BglI GCCn_nnn'nGGC
Cuts at: 307 307
Size: 3821
BglII A'GATC_T
Cuts at: 143 1336 3373 143
Size: 1193 2037 591
Fragments arranged by size:
2037 1193 591
BlpI GC'TnA_GC
Cuts at: 933 933
Size: 3821
Bme1580I G_kGCm'C
Cuts at: 1446 1539 2595 1446
Size: 93 1056 2672
Fragments arranged by size:
2672 1056 93
BmgBI CAC'GTC
Cuts at: 516 516
Size: 3821
BmrI ACTGGGnnnn_n'
Cuts at: 1033 1114 1449 3593 1033
Size: 81 335 2144 1261
Fragments arranged by size:
2144 1261 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 3821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 635 587
Size: 48 3773
Bpu10I CC'TnA_GC
Cuts at: 788 788
Size: 3821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 1876 2368 2666 997
Size: 879 492 298 2152
Fragments arranged by size:
2152 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3525 3525
Size: 3821
BsaAI yAC'GTr
Cuts at: 1174 1174
Size: 3821
BsaBI GATnn'nnATC
Cuts at: 6 323 1335 3372 6
Size: 317 1012 2037 455
Fragments arranged by size:
2037 1012 455 317
BsaHI Gr'CG_yC
Cuts at: 179 1252 1500 2202 3698 179
Size: 1073 248 702 1496 302
Fragments arranged by size:
1496 1073 702 302 248
BsaWI w'CCGG_w
Cuts at: 1010 1531 2209 2483 2630 3041 1010
Size: 521 678 274 147 411 1790
Fragments arranged by size:
1790 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 235
Size: 3821
BseYI C'CCAG_C
Cuts at: 676 1246 2004 2581 3077 676
Size: 570 758 577 496 1420
Fragments arranged by size:
1420 758 577 570 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 511
Size: 3821
BsiEI CG_ry'CG
Cuts at: 104 243 1363 1409 2617 104
Size: 139 1120 46 1208 1308
Fragments arranged by size:
1308 1208 1120 139 46
BsiHKAI G_wGCw'C
Cuts at: 835 1613 1803 2595 835
Size: 778 190 792 2061
Fragments arranged by size:
2061 792 778 190
BsmI GAATG_Cn'
Cuts at: 815 815
Size: 3821
Bsp1286I G_dGCh'C
Cuts at: 835 1446 1539 1613 1803 1865 2595 835
Size: 611 93 74 190 62 730 2061
Fragments arranged by size:
2061 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3041 3041
Size: 3821
BspHI T'CATG_A
Cuts at: 2997 2997
Size: 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 468 871 1085 1387 1768 468
Size: 403 214 302 381 2521
Fragments arranged by size:
2521 403 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2113 2167 3651 3816 50
Size: 2063 54 1484 165 55
Fragments arranged by size:
2063 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1733 1733
Size: 3821
BsrFI r'CCGG_y
Cuts at: 242 1819 2000 2209 242
Size: 1577 181 209 1854
Fragments arranged by size:
1854 1577 209 181
BssSI C'ACGA_G
Cuts at: 2092 2450 2092
Size: 358 3463
BstAPI GCAn_nnn'nTGC
Cuts at: 598 754 757 1198 598
Size: 156 3 441 3221
Fragments arranged by size:
3221 441 156 3
BstBI TT'CG_AA
Cuts at: 2182 2182
Size: 3821
BstYI r'GATC_y
Cuts at: 1 143 318 846 1313 1336 1671 1917 2929
Size: 142 175 528 467 23 335 246 1012
Cuts at: 2929 3015 3027 3373 1
Size: 86 12 346 449
Fragments arranged by size:
1012 528 467 449 346 335 246 175
142 86 23 12
BtgI C'CryG_G
Cuts at: 281 281
Size: 3821
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1771 1952 2115 1771
Size: 181 163 3477
Fragments arranged by size:
3477 181 163
BtsI GCAGTG_nn'
Cuts at: 1064 1064
Size: 3821
DraI TTT'AAA
Cuts at: 820 820
Size: 3821
DrdI GACnn_nn'nnGTC
Cuts at: 255 1527 2385 255
Size: 1272 858 1691
Fragments arranged by size:
1691 1272 858
EaeI y'GGCC_r
Cuts at: 774 1406 1580 1971 1998 3343 774
Size: 632 174 391 27 1345 1252
Fragments arranged by size:
1345 1252 632 391 174 27
EagI C'GGCC_G
Cuts at: 1406 1406
Size: 3821
EarI CTCTTCn'nnn_
Cuts at: 1844 2054 1844
Size: 210 3611
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2029 2339 2485 3631 2029
Size: 310 146 1146 2219
Fragments arranged by size:
2219 1146 310 146
EcoICRI GAG'CTC
Cuts at: 833 833
Size: 3821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 635 1646 2078 2825 3093 587
Size: 48 1011 432 747 268 1315
Fragments arranged by size:
1315 1011 747 432 268 48
EcoO109I rG'GnC_Cy
Cuts at: 308 960 308
Size: 652 3169
EcoRI G'AATT_C
Cuts at: 825 825
Size: 3821
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1840 1840
Size: 3821
FspI TGC'GCA
Cuts at: 190 1602 190
Size: 1412 2409
HaeII r_GCGC'y
Cuts at: 1255 1503 2525 3504 1255
Size: 248 1022 979 1572
Fragments arranged by size:
1572 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1663 1693 1695 1725 1663
Size: 30 2 30 3759
Fragments arranged by size:
3759 30 30 2
HincII GTy'rAC
Cuts at: 860 860
Size: 3821
KasI G'GCGC_C
Cuts at: 1251 1499 1251
Size: 248 3573
KpnI G_GTAC'C
Cuts at: 841 841
Size: 3821
MluI A'CGCG_T
Cuts at: 3254 3254
Size: 3821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2492 2676 2492
Size: 184 3637
MscI TGG'CCA
Cuts at: 776 1582 776
Size: 806 3015
MslI CAynn'nnrTG
Cuts at: 1810 1937 1810
Size: 127 3694
MspA1I CmG'CkG
Cuts at: 532 595 616 655 718 733 745 754 763
Size: 63 21 39 63 15 12 9 9
Cuts at: 763 795 932 1246 1606 2619 2864 532
Size: 32 137 314 360 1013 245 1489
Fragments arranged by size:
1489 1013 360 314 245 137 63 63
39 32 21 15 12 9 9
NaeI GCC'GGC
Cuts at: 2002 2002
Size: 3821
NarI GG'CG_CC
Cuts at: 1252 1500 1252
Size: 248 3573
NcoI C'CATG_G
Cuts at: 281 281
Size: 3821
NgoMIV G'CCGG_C
Cuts at: 2000 2000
Size: 3821
NspI r_CATG'y
Cuts at: 874 1374 2281 874
Size: 500 907 2414
Fragments arranged by size:
2414 907 500
PciI A'CATG_T
Cuts at: 2277 2277
Size: 3821
PmeI GTTT'AAAC
Cuts at: 820 820
Size: 3821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2992 2960
Size: 32 3789
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 349 381 349
Size: 32 3789
PstI C_TGCA'G
Cuts at: 663 750 768 800 868 1553 663
Size: 87 18 32 68 685 2931
Fragments arranged by size:
2931 685 87 68 32 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3821
PvuII CAG'CTG
Cuts at: 532 595 655 733 754 795 1246 1606 532
Size: 63 60 78 21 41 451 360 2747
Fragments arranged by size:
2747 451 360 78 63 60 41 21
RsrII CG'GwC_CG
Cuts at: 2016 2016
Size: 3821
SacI G_AGCT'C
Cuts at: 835 835
Size: 3821
SalI G'TCGA_C
Cuts at: 858 858
Size: 3821
SapI GCTCTTCn'nnn_
Cuts at: 1844 2054 1844
Size: 210 3611
SbfI CC_TGCA'GG
Cuts at: 868 868
Size: 3821
ScaI AGT'ACT
Cuts at: 196 3338 196
Size: 3142 679
SexAI A'CCwGG_T
Cuts at: 396 396
Size: 3821
SfcI C'TryA_G
Cuts at: 33 659 746 764 796 864 1549 2542 2733
Size: 626 87 18 32 68 685 993 191
Cuts at: 2733 33
Size: 1121
Fragments arranged by size:
1121 993 685 626 191 87 68 32
18
SfoI GGC'GCC
Cuts at: 1253 1501 1253
Size: 248 3573
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3821
SmaI CCC'GGG
Cuts at: 262 843 262
Size: 581 3240
SmlI C'TyrA_G
Cuts at: 976 1891 2383 2645 976
Size: 915 492 262 2152
Fragments arranged by size:
2152 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3821
SphI G_CATG'C
Cuts at: 874 1374 874
Size: 500 3321
StyI C'CwwG_G
Cuts at: 281 382 955 1309 281
Size: 101 573 354 2793
Fragments arranged by size:
2793 573 354 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 821 1404 1537 1996 2205 821
Size: 583 133 459 209 2437
Fragments arranged by size:
2437 583 459 209 133
TatI w'GTAC_w
Cuts at: 194 3336 194
Size: 3142 679
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3338 3370 3338
Size: 32 3789
Tth111I GACn'n_nGTC
Cuts at: 1618 1618
Size: 3821
XbaI T'CTAG_A
Cuts at: 852 852
Size: 3821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 357 1316 357
Size: 959 2862
XmaI C'CCGG_G
Cuts at: 260 841 260
Size: 581 3240
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI