Restriction map for
pF1KE0664
(Circular) (Six-base) MAPSORT of: pF1KE0664.seq Check: 1990 from: 1 to: 4289
pF1KE0664 4289 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1327 1327
Size: 4289
Acc65I G'GTAC_C
Cuts at: 1305 1305
Size: 4289
AclI AA'CG_TT
Cuts at: 4129 4129
Size: 4289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2114 2546 3293 3561 2114
Size: 432 747 268 2842
Fragments arranged by size:
2842 747 432 268
AflIII A'CryG_T
Cuts at: 1641 2745 3722 1641
Size: 1104 977 2208
Fragments arranged by size:
2208 1104 977
AgeI A'CCGG_T
Cuts at: 2677 2677
Size: 4289
AleI CACnn'nnGTG
Cuts at: 845 845
Size: 4289
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 307 339 307
Size: 32 4257
AlwNI CAG_nnn'CTG
Cuts at: 1594 3161 1594
Size: 1567 2722
ApaI G_GGCC'C
Cuts at: 394 488 540 613 651 733 394
Size: 94 52 73 38 82 3950
Fragments arranged by size:
3950 94 82 73 52 38
ApaLI G'TGCA_C
Cuts at: 3059 3059
Size: 4289
ApoI r'AATT_y
Cuts at: 1293 3642 3898 1293
Size: 2349 256 1684
Fragments arranged by size:
2349 1684 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4289
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
AvaI C'yCGr_G
Cuts at: 126 420 476 768 791 1116 1309 126
Size: 294 56 292 23 325 193 3106
Fragments arranged by size:
3106 325 294 292 193 56 23
BamHI G'GATC_C
Cuts at: 1314 1314
Size: 4289
BanI G'GyrC_C
Cuts at: 979 1221 1305 1719 1967 2002 979
Size: 242 84 414 248 35 3266
Fragments arranged by size:
3266 414 248 242 84 35
BanII G_rGCy'C
Cuts at: 394 488 540 613 651 733 953 1157 1303
Size: 94 52 73 38 82 220 204 146
Cuts at: 1303 2333 394
Size: 1030 2350
Fragments arranged by size:
2350 1030 220 204 146 94 82 73
52 38
BbeI G_GCGC'C
Cuts at: 983 1723 1971 983
Size: 740 248 3301
Fragments arranged by size:
3301 740 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 411 445 411
Size: 34 4255
BciVI GTATCCnnnnn_n'
Cuts at: 2187 2948 4289 2187
Size: 761 1341 2187
Fragments arranged by size:
2187 1341 761
BclI T'GATC_A
Cuts at: 1809 1809
Size: 4289
BglI GCCn_nnn'nGGC
Cuts at: 389 1211 389
Size: 822 3467
BglII A'GATC_T
Cuts at: 1804 3841 1804
Size: 2037 2252
BlpI GC'TnA_GC
Cuts at: 1401 1401
Size: 4289
Bme1580I G_kGCm'C
Cuts at: 394 488 540 613 651 733 1226 1914 2007
Size: 94 52 73 38 82 493 688 93
Cuts at: 2007 3063 394
Size: 1056 1620
Fragments arranged by size:
1620 1056 688 493 94 93 82 73
52 38
BmrI ACTGGGnnnn_n'
Cuts at: 1501 1582 1917 4061 1501
Size: 81 335 2144 1729
Fragments arranged by size:
2144 1729 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 765 2656 765
Size: 1891 2398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 516 828 516
Size: 312 3977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 888 1465 2344 2836 3134 888
Size: 577 879 492 298 2043
Fragments arranged by size:
2043 879 577 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3993 3993
Size: 4289
BsaAI yAC'GTr
Cuts at: 1642 1642
Size: 4289
BsaBI GATnn'nnATC
Cuts at: 6 1803 3840 6
Size: 1797 2037 455
Fragments arranged by size:
2037 1797 455
BsaHI Gr'CG_yC
Cuts at: 290 980 1720 1968 2670 4166 290
Size: 690 740 248 702 1496 413
Fragments arranged by size:
1496 740 702 690 413 248
BsaWI w'CCGG_w
Cuts at: 1478 1999 2677 2951 3098 3509 1478
Size: 521 678 274 147 411 2258
Fragments arranged by size:
2258 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 826 856 826
Size: 30 4259
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1031 1034 1048 1277 1280 1031
Size: 3 14 229 3 4040
Fragments arranged by size:
4040 229 14 3 3
BseYI C'CCAG_C
Cuts at: 1187 1714 2472 3049 3545 1187
Size: 527 758 577 496 1931
Fragments arranged by size:
1931 758 577 527 496
BsiEI CG_ry'CG
Cuts at: 104 417 885 909 1074 1110 1831 1877 3085
Size: 313 468 24 165 36 721 46 1208
Cuts at: 3085 104
Size: 1308
Fragments arranged by size:
1308 1208 721 468 313 165 46 36
24
BsiHKAI G_wGCw'C
Cuts at: 221 1303 2081 2271 3063 221
Size: 1082 778 190 792 1447
Fragments arranged by size:
1447 1082 792 778 190
BsmI GAATG_Cn'
Cuts at: 641 641
Size: 4289
BsmBI CGTCTCn'nnnn_
Cuts at: 662 752 881 662
Size: 90 129 4070
Fragments arranged by size:
4070 129 90
Bsp1286I G_dGCh'C
Cuts at: 221 394 488 540 613 651 733 953 1157
Size: 173 94 52 73 38 82 220 204
Cuts at: 1157 1226 1303 1914 2007 2081 2271 2333 3063
Size: 69 77 611 93 74 190 62 730
Cuts at: 3063 221
Size: 1447
Fragments arranged by size:
1447 730 611 220 204 190 173 94
93 82 77 74 73 69 62 52
38
BspEI T'CCGG_A
Cuts at: 3509 3509
Size: 4289
BspHI T'CATG_A
Cuts at: 3465 3465
Size: 4289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 530 1339 1553 1855 2236 530
Size: 809 214 302 381 2583
Fragments arranged by size:
2583 809 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2581 2635 4119 4284 50
Size: 2531 54 1484 165 55
Fragments arranged by size:
2531 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2201 2201
Size: 4289
BsrFI r'CCGG_y
Cuts at: 366 383 1205 2287 2468 2677 366
Size: 17 822 1082 181 209 1978
Fragments arranged by size:
1978 1082 822 209 181 17
BssSI C'ACGA_G
Cuts at: 2560 2918 2560
Size: 358 3931
BstAPI GCAn_nnn'nTGC
Cuts at: 1666 1666
Size: 4289
BstBI TT'CG_AA
Cuts at: 2650 2650
Size: 4289
BstXI CCAn_nnnn'nTGG
Cuts at: 348 348
Size: 4289
BstYI r'GATC_y
Cuts at: 1 1314 1781 1804 2139 2385 3397 3483 3495
Size: 1313 467 23 335 246 1012 86 12
Cuts at: 3495 3841 1
Size: 346 449
Fragments arranged by size:
1313 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 411 441 460 733 1166 107
Size: 304 30 19 273 433 3230
Fragments arranged by size:
3230 433 304 273 30 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2239 2420 2583 2239
Size: 181 163 3945
Fragments arranged by size:
3945 181 163
BtsI GCAGTG_nn'
Cuts at: 1532 1532
Size: 4289
DraI TTT'AAA
Cuts at: 1288 1288
Size: 4289
DraIII CAC_nnn'GTG
Cuts at: 1134 1134
Size: 4289
DrdI GACnn_nn'nnGTC
Cuts at: 1995 2853 1995
Size: 858 3431
EaeI y'GGCC_r
Cuts at: 201 653 837 1071 1098 1874 2048 2439 2466
Size: 452 184 234 27 776 174 391 27
Cuts at: 2466 3811 201
Size: 1345 679
Fragments arranged by size:
1345 776 679 452 391 234 184 174
27 27
EagI C'GGCC_G
Cuts at: 1071 1874 1071
Size: 803 3486
EarI CTCTTCn'nnn_
Cuts at: 2312 2522 2312
Size: 210 4079
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 526 1247 2497 2807 2953 4099 526
Size: 721 1250 310 146 1146 716
Fragments arranged by size:
1250 1146 721 716 310 146
EcoICRI GAG'CTC
Cuts at: 1301 1301
Size: 4289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 516 828 2114 2546 3293 3561 516
Size: 312 1286 432 747 268 1244
Fragments arranged by size:
1286 1244 747 432 312 268
EcoO109I rG'GnC_Cy
Cuts at: 147 390 536 537 647 648 1428 147
Size: 243 146 1 110 1 780 3008
Fragments arranged by size:
3008 780 243 146 110 1 1
EcoRI G'AATT_C
Cuts at: 1293 1293
Size: 4289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 4289
FspI TGC'GCA
Cuts at: 2070 2070
Size: 4289
HaeII r_GCGC'y
Cuts at: 983 1723 1971 2993 3972 983
Size: 740 248 1022 979 1300
Fragments arranged by size:
1300 1022 979 740 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 661 693 751 783 1099 1131 2131 2161 2163
Size: 32 58 32 316 32 1000 30 2
Cuts at: 2163 2193 661
Size: 30 2757
Fragments arranged by size:
2757 1000 316 58 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1328 1328
Size: 4289
KasI G'GCGC_C
Cuts at: 979 1719 1967 979
Size: 740 248 3301
Fragments arranged by size:
3301 740 248
KpnI G_GTAC'C
Cuts at: 1309 1309
Size: 4289
MluI A'CGCG_T
Cuts at: 3722 3722
Size: 4289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2960 3144 2960
Size: 184 4105
MscI TGG'CCA
Cuts at: 2050 2050
Size: 4289
MslI CAynn'nnrTG
Cuts at: 346 845 2278 2405 346
Size: 499 1433 127 2230
Fragments arranged by size:
2230 1433 499 127
MspA1I CmG'CkG
Cuts at: 413 443 462 626 702 735 1067 1400 1714
Size: 30 19 164 76 33 332 333 314
Cuts at: 1714 2074 3087 3332 413
Size: 360 1013 245 1370
Fragments arranged by size:
1370 1013 360 333 332 314 245 164
76 33 30 19
NaeI GCC'GGC
Cuts at: 385 2470 385
Size: 2085 2204
NarI GG'CG_CC
Cuts at: 980 1720 1968 980
Size: 740 248 3301
Fragments arranged by size:
3301 740 248
NcoI C'CATG_G
Cuts at: 107 1166 107
Size: 1059 3230
NgoMIV G'CCGG_C
Cuts at: 383 2468 383
Size: 2085 2204
NotI GC'GGCC_GC
Cuts at: 1071 1071
Size: 4289
NruI TCG'CGA
Cuts at: 905 905
Size: 4289
NspI r_CATG'y
Cuts at: 1342 1842 2749 1342
Size: 500 907 2882
Fragments arranged by size:
2882 907 500
PasI CC'CwG_GG
Cuts at: 1226 1226
Size: 4289
PciI A'CATG_T
Cuts at: 2745 2745
Size: 4289
PflMI CCAn_nnn'nTGG
Cuts at: 1150 1150
Size: 4289
PfoI T'CCnGG_A
Cuts at: 851 851
Size: 4289
PmeI GTTT'AAAC
Cuts at: 1288 1288
Size: 4289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 4257
PshAI GACnn'nnGTC
Cuts at: 860 860
Size: 4289
PspOMI G'GGCC_C
Cuts at: 390 484 536 609 647 729 390
Size: 94 52 73 38 82 3950
Fragments arranged by size:
3950 94 82 73 52 38
PspXI vC'TCGA_Gb
Cuts at: 768 1116 768
Size: 348 3941
PstI C_TGCA'G
Cuts at: 1219 1336 2021 1219
Size: 117 685 3487
Fragments arranged by size:
3487 685 117
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4289
PvuII CAG'CTG
Cuts at: 1714 2074 1714
Size: 360 3929
RsrII CG'GwC_CG
Cuts at: 2484 2484
Size: 4289
SacI G_AGCT'C
Cuts at: 1303 1303
Size: 4289
SacII CC_GC'GG
Cuts at: 414 444 463 736 414
Size: 30 19 273 3967
Fragments arranged by size:
3967 273 30 19
SalI G'TCGA_C
Cuts at: 1326 1326
Size: 4289
SapI GCTCTTCn'nnn_
Cuts at: 2312 2522 2312
Size: 210 4079
SbfI CC_TGCA'GG
Cuts at: 1336 1336
Size: 4289
ScaI AGT'ACT
Cuts at: 3806 3806
Size: 4289
SfcI C'TryA_G
Cuts at: 33 876 1062 1215 1332 2017 3010 3201 33
Size: 843 186 153 117 685 993 191 1121
Fragments arranged by size:
1121 993 843 685 191 186 153 117
SfoI GGC'GCC
Cuts at: 981 1721 1969 981
Size: 740 248 3301
Fragments arranged by size:
3301 740 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
SmaI CCC'GGG
Cuts at: 128 478 793 1311 128
Size: 350 315 518 3106
Fragments arranged by size:
3106 518 350 315
SmlI C'TyrA_G
Cuts at: 768 867 1116 1444 2359 2851 3113 768
Size: 99 249 328 915 492 262 1944
Fragments arranged by size:
1944 915 492 328 262 249 99
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4289
SphI G_CATG'C
Cuts at: 1342 1842 1342
Size: 500 3789
StyI C'CwwG_G
Cuts at: 107 293 1166 1423 1777 107
Size: 186 873 257 354 2619
Fragments arranged by size:
2619 873 354 257 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 248 838 899 1872 2005 2464 2673 248
Size: 590 61 973 133 459 209 1864
Fragments arranged by size:
1864 973 590 459 209 133 61
TatI w'GTAC_w
Cuts at: 818 3804 818
Size: 2986 1303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3806 3838 3806
Size: 32 4257
Tth111I GACn'n_nGTC
Cuts at: 2086 2086
Size: 4289
XbaI T'CTAG_A
Cuts at: 1320 1320
Size: 4289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1784 1784
Size: 4289
XhoI C'TCGA_G
Cuts at: 768 1116 768
Size: 348 3941
XmaI C'CCGG_G
Cuts at: 126 476 791 1309 126
Size: 350 315 518 3106
Fragments arranged by size:
3106 518 350 315
XmnI GAAnn'nnTTC
Cuts at: 136 136
Size: 4289
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsgI
BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NsiI PacI PmlI PpuMI PsiI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
ZraI