Restriction map for
pF1KE0669
(Circular) (Six-base) MAPSORT of: pF1KE0669.seq Check: 4420 from: 1 to: 3860
pF1KE0669 3860 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 898 898
Size: 3860
Acc65I G'GTAC_C
Cuts at: 876 876
Size: 3860
AclI AA'CG_TT
Cuts at: 3700 3700
Size: 3860
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 681 1685 2117 2864 3132 681
Size: 1004 432 747 268 1409
Fragments arranged by size:
1409 1004 747 432 268
AflIII A'CryG_T
Cuts at: 1212 2316 3293 1212
Size: 1104 977 1779
Fragments arranged by size:
1779 1104 977
AgeI A'CCGG_T
Cuts at: 2248 2248
Size: 3860
AleI CACnn'nnGTG
Cuts at: 379 379
Size: 3860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 397 535 397
Size: 138 3722
AlwNI CAG_nnn'CTG
Cuts at: 486 543 1165 2732 486
Size: 57 622 1567 1614
Fragments arranged by size:
1614 1567 622 57
ApaI G_GGCC'C
Cuts at: 761 819 761
Size: 58 3802
ApaLI G'TGCA_C
Cuts at: 2630 2630
Size: 3860
ApoI r'AATT_y
Cuts at: 864 3213 3469 864
Size: 2349 256 1255
Fragments arranged by size:
2349 1255 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3860
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3860
AvaI C'yCGr_G
Cuts at: 365 709 880 365
Size: 344 171 3345
Fragments arranged by size:
3345 344 171
BamHI G'GATC_C
Cuts at: 885 885
Size: 3860
BanI G'GyrC_C
Cuts at: 747 876 1290 1538 1573 747
Size: 129 414 248 35 3034
Fragments arranged by size:
3034 414 248 129 35
BanII G_rGCy'C
Cuts at: 278 716 761 819 874 1904 278
Size: 438 45 58 55 1030 2234
Fragments arranged by size:
2234 1030 438 58 55 45
BbeI G_GCGC'C
Cuts at: 1294 1542 1294
Size: 248 3612
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 244 278 244
Size: 34 3826
BciVI GTATCCnnnnn_n'
Cuts at: 1758 2519 3860 1758
Size: 761 1341 1758
Fragments arranged by size:
1758 1341 761
BclI T'GATC_A
Cuts at: 1380 1380
Size: 3860
BglI GCCn_nnn'nGGC
Cuts at: 636 636
Size: 3860
BglII A'GATC_T
Cuts at: 1375 3412 1375
Size: 2037 1823
BlpI GC'TnA_GC
Cuts at: 835 972 835
Size: 137 3723
Bme1580I G_kGCm'C
Cuts at: 648 761 819 1485 1578 2634 648
Size: 113 58 666 93 1056 1874
Fragments arranged by size:
1874 1056 666 113 93 58
BmrI ACTGGGnnnn_n'
Cuts at: 531 574 635 1072 1153 1488 3632 531
Size: 43 61 437 81 335 2144 759
Fragments arranged by size:
2144 759 437 335 81 61 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 3860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 496 699 316
Size: 180 203 3477
Fragments arranged by size:
3477 203 180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 1915 2407 2705 1036
Size: 879 492 298 2191
Fragments arranged by size:
2191 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3564 3564
Size: 3860
BsaAI yAC'GTr
Cuts at: 381 499 1213 381
Size: 118 714 3028
Fragments arranged by size:
3028 714 118
BsaBI GATnn'nnATC
Cuts at: 6 1374 3411 6
Size: 1368 2037 455
Fragments arranged by size:
2037 1368 455
BsaHI Gr'CG_yC
Cuts at: 152 284 1291 1539 2241 3737 152
Size: 132 1007 248 702 1496 275
Fragments arranged by size:
1496 1007 702 275 248 132
BsaWI w'CCGG_w
Cuts at: 1049 1570 2248 2522 2669 3080 1049
Size: 521 678 274 147 411 1829
Fragments arranged by size:
1829 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 346 346
Size: 3860
BseYI C'CCAG_C
Cuts at: 416 524 530 603 615 1285 2043 2620 3116
Size: 108 6 73 12 670 758 577 496
Cuts at: 3116 416
Size: 1160
Fragments arranged by size:
1160 758 670 577 496 108 73 12
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 772 288
Size: 484 3376
BsiEI CG_ry'CG
Cuts at: 104 1402 1448 2656 104
Size: 1298 46 1208 1308
Fragments arranged by size:
1308 1298 1208 46
BsiHKAI G_wGCw'C
Cuts at: 685 874 1652 1842 2634 685
Size: 189 778 190 792 1911
Fragments arranged by size:
1911 792 778 190 189
Bsp1286I G_dGCh'C
Cuts at: 278 648 685 716 761 819 874 1485 1578
Size: 370 37 31 45 58 55 611 93
Cuts at: 1578 1652 1842 1904 2634 278
Size: 74 190 62 730 1504
Fragments arranged by size:
1504 730 611 370 190 93 74 62
58 55 45 37 31
BspEI T'CCGG_A
Cuts at: 3080 3080
Size: 3860
BspHI T'CATG_A
Cuts at: 3036 3036
Size: 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 461 910 1124 1426 1807 461
Size: 449 214 302 381 2514
Fragments arranged by size:
2514 449 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2152 2206 3690 3855 50
Size: 2102 54 1484 165 55
Fragments arranged by size:
2102 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1772 1772
Size: 3860
BsrFI r'CCGG_y
Cuts at: 212 635 1858 2039 2248 212
Size: 423 1223 181 209 1824
Fragments arranged by size:
1824 1223 423 209 181
BsrGI T'GTAC_A
Cuts at: 179 179
Size: 3860
BssHII G'CGCG_C
Cuts at: 370 370
Size: 3860
BssSI C'ACGA_G
Cuts at: 790 2131 2489 790
Size: 1341 358 2161
Fragments arranged by size:
2161 1341 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1237 1237
Size: 3860
BstBI TT'CG_AA
Cuts at: 2221 2221
Size: 3860
BstYI r'GATC_y
Cuts at: 1 809 885 1352 1375 1710 1956 2968 3054
Size: 808 76 467 23 335 246 1012 86
Cuts at: 3054 3066 3412 1
Size: 12 346 449
Fragments arranged by size:
1012 808 467 449 346 335 246 86
76 23 12
BtgI C'CryG_G
Cuts at: 278 407 753 278
Size: 129 346 3385
Fragments arranged by size:
3385 346 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1810 1991 2154 1810
Size: 181 163 3516
Fragments arranged by size:
3516 181 163
BtsI GCAGTG_nn'
Cuts at: 1103 1103
Size: 3860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 171 206 171
Size: 35 3825
DraI TTT'AAA
Cuts at: 859 859
Size: 3860
DrdI GACnn_nn'nnGTC
Cuts at: 1566 2424 1566
Size: 858 3002
EaeI y'GGCC_r
Cuts at: 386 801 825 1445 1619 2010 2037 3382 386
Size: 415 24 620 174 391 27 1345 864
Fragments arranged by size:
1345 864 620 415 391 174 27 24
EagI C'GGCC_G
Cuts at: 1445 1445
Size: 3860
EarI CTCTTCn'nnn_
Cuts at: 250 1883 2093 250
Size: 1633 210 2017
Fragments arranged by size:
2017 1633 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2068 2378 2524 3670 2068
Size: 310 146 1146 2258
Fragments arranged by size:
2258 1146 310 146
EcoICRI GAG'CTC
Cuts at: 872 872
Size: 3860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 496 681 699 1685 2117 2864 3132 316
Size: 180 185 18 986 432 747 268 1044
Fragments arranged by size:
1044 986 747 432 268 185 180 18
EcoO109I rG'GnC_Cy
Cuts at: 362 426 758 816 999 362
Size: 64 332 58 183 3223
Fragments arranged by size:
3223 332 183 64 58
EcoRI G'AATT_C
Cuts at: 864 864
Size: 3860
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1879 1879
Size: 3860
FspI TGC'GCA
Cuts at: 1641 1641
Size: 3860
HaeII r_GCGC'y
Cuts at: 219 1294 1542 2564 3543 219
Size: 1075 248 1022 979 536
Fragments arranged by size:
1075 1022 979 536 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1702 1732 1734 1764 1702
Size: 30 2 30 3798
Fragments arranged by size:
3798 30 30 2
HincII GTy'rAC
Cuts at: 899 899
Size: 3860
KasI G'GCGC_C
Cuts at: 1290 1538 1290
Size: 248 3612
KpnI G_GTAC'C
Cuts at: 880 880
Size: 3860
MluI A'CGCG_T
Cuts at: 3293 3293
Size: 3860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2531 2715 2531
Size: 184 3676
MscI TGG'CCA
Cuts at: 803 827 1621 803
Size: 24 794 3042
Fragments arranged by size:
3042 794 24
MslI CAynn'nnrTG
Cuts at: 379 1849 1976 379
Size: 1470 127 2263
Fragments arranged by size:
2263 1470 127
MspA1I CmG'CkG
Cuts at: 391 486 971 1285 1645 2658 2903 391
Size: 95 485 314 360 1013 245 1348
Fragments arranged by size:
1348 1013 485 360 314 245 95
NaeI GCC'GGC
Cuts at: 637 2041 637
Size: 1404 2456
NarI GG'CG_CC
Cuts at: 1291 1539 1291
Size: 248 3612
NcoI C'CATG_G
Cuts at: 407 407
Size: 3860
NgoMIV G'CCGG_C
Cuts at: 635 2039 635
Size: 1404 2456
NspI r_CATG'y
Cuts at: 243 913 1413 2320 243
Size: 670 500 907 1783
Fragments arranged by size:
1783 907 670 500
PciI A'CATG_T
Cuts at: 2316 2316
Size: 3860
PfoI T'CCnGG_A
Cuts at: 293 293
Size: 3860
PmeI GTTT'AAAC
Cuts at: 859 859
Size: 3860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2999 3031 2999
Size: 32 3828
PpuMI rG'GwC_Cy
Cuts at: 362 362
Size: 3860
PspOMI G'GGCC_C
Cuts at: 757 815 757
Size: 58 3802
PstI C_TGCA'G
Cuts at: 907 1592 907
Size: 685 3175
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3860
PvuII CAG'CTG
Cuts at: 486 1285 1645 486
Size: 799 360 2701
Fragments arranged by size:
2701 799 360
RsrII CG'GwC_CG
Cuts at: 2055 2055
Size: 3860
SacI G_AGCT'C
Cuts at: 874 874
Size: 3860
SalI G'TCGA_C
Cuts at: 897 897
Size: 3860
SanDI GG'GwC_CC
Cuts at: 362 362
Size: 3860
SapI GCTCTTCn'nnn_
Cuts at: 250 1883 2093 250
Size: 1633 210 2017
Fragments arranged by size:
2017 1633 210
SbfI CC_TGCA'GG
Cuts at: 907 907
Size: 3860
ScaI AGT'ACT
Cuts at: 3377 3377
Size: 3860
SfcI C'TryA_G
Cuts at: 33 903 1588 2581 2772 33
Size: 870 685 993 191 1121
Fragments arranged by size:
1121 993 870 685 191
SfoI GGC'GCC
Cuts at: 1292 1540 1292
Size: 248 3612
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3860
SgrAI Cr'CCGG_yG
Cuts at: 212 212
Size: 3860
SmaI CCC'GGG
Cuts at: 367 882 367
Size: 515 3345
SmlI C'TyrA_G
Cuts at: 1015 1930 2422 2684 1015
Size: 915 492 262 2191
Fragments arranged by size:
2191 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3860
SphI G_CATG'C
Cuts at: 913 1413 913
Size: 500 3360
StuI AGG'CCT
Cuts at: 702 702
Size: 3860
StyI C'CwwG_G
Cuts at: 407 656 994 1348 407
Size: 249 338 354 2919
Fragments arranged by size:
2919 354 338 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1443 1576 2035 2244 1443
Size: 133 459 209 3059
Fragments arranged by size:
3059 459 209 133
TatI w'GTAC_w
Cuts at: 179 3375 179
Size: 3196 664
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 3828
Tth111I GACn'n_nGTC
Cuts at: 1657 1657
Size: 3860
XbaI T'CTAG_A
Cuts at: 891 891
Size: 3860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1355 1355
Size: 3860
XmaI C'CCGG_G
Cuts at: 365 880 365
Size: 515 3345
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI