Restriction map for
pF1KE0672
(Circular) (Six-base) MAPSORT of: pF1KE0672.seq Check: 8961 from: 1 to: 4142
pF1KE0672 4142 bp
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 895 895
Size: 4142
AccI GT'mk_AC
Cuts at: 149 1180 149
Size: 1031 3111
Acc65I G'GTAC_C
Cuts at: 731 1158 731
Size: 427 3715
AclI AA'CG_TT
Cuts at: 3982 3982
Size: 4142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 753 1967 2399 3146 3414 139
Size: 614 1214 432 747 268 867
Fragments arranged by size:
1214 867 747 614 432 268
AflIII A'CryG_T
Cuts at: 1494 2598 3575 1494
Size: 1104 977 2061
Fragments arranged by size:
2061 1104 977
AgeI A'CCGG_T
Cuts at: 2530 2530
Size: 4142
AlwNI CAG_nnn'CTG
Cuts at: 780 1447 3014 780
Size: 667 1567 1908
Fragments arranged by size:
1908 1567 667
ApaI G_GGCC'C
Cuts at: 699 699
Size: 4142
ApaLI G'TGCA_C
Cuts at: 505 2912 505
Size: 2407 1735
ApoI r'AATT_y
Cuts at: 1146 3495 3751 1146
Size: 2349 256 1537
Fragments arranged by size:
2349 1537 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4142
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
AvaI C'yCGr_G
Cuts at: 174 1162 174
Size: 988 3154
BamHI G'GATC_C
Cuts at: 1167 1167
Size: 4142
BanI G'GyrC_C
Cuts at: 178 472 616 731 851 905 1158 1572 1820
Size: 294 144 115 120 54 253 414 248
Cuts at: 1820 1855 178
Size: 35 2465
Fragments arranged by size:
2465 414 294 253 248 144 120 115
54 35
BanII G_rGCy'C
Cuts at: 217 355 656 699 767 1156 2186 217
Size: 138 301 43 68 389 1030 2173
Fragments arranged by size:
2173 1030 389 301 138 68 43
BbeI G_GCGC'C
Cuts at: 476 1576 1824 476
Size: 1100 248 2794
Fragments arranged by size:
2794 1100 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4108
BciVI GTATCCnnnnn_n'
Cuts at: 2040 2801 4142 2040
Size: 761 1341 2040
Fragments arranged by size:
2040 1341 761
BclI T'GATC_A
Cuts at: 1662 1662
Size: 4142
BglI GCCn_nnn'nGGC
Cuts at: 1106 1106
Size: 4142
BglII A'GATC_T
Cuts at: 1657 3694 1657
Size: 2037 2105
BlpI GC'TnA_GC
Cuts at: 1254 1254
Size: 4142
Bme1580I G_kGCm'C
Cuts at: 509 699 1767 1860 2916 509
Size: 190 1068 93 1056 1735
Fragments arranged by size:
1735 1068 1056 190 93
BmrI ACTGGGnnnn_n'
Cuts at: 1354 1435 1770 3914 1354
Size: 81 335 2144 1582
Fragments arranged by size:
2144 1582 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 231 441 819 1089 2509 231
Size: 210 378 270 1420 1864
Fragments arranged by size:
1864 1420 378 270 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 880 355
Size: 525 3617
Bpu10I CC'TnA_GC
Cuts at: 761 761
Size: 4142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 502 1318 2197 2689 2987 220
Size: 282 816 879 492 298 1375
Fragments arranged by size:
1375 879 816 492 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 576 860 3846 576
Size: 284 2986 872
Fragments arranged by size:
2986 872 284
BsaAI yAC'GTr
Cuts at: 1495 1495
Size: 4142
BsaBI GATnn'nnATC
Cuts at: 6 1656 3693 6
Size: 1650 2037 455
Fragments arranged by size:
2037 1650 455
BsaHI Gr'CG_yC
Cuts at: 473 704 1573 1821 2523 4019 473
Size: 231 869 248 702 1496 596
Fragments arranged by size:
1496 869 702 596 248 231
BsaWI w'CCGG_w
Cuts at: 971 1037 1331 1852 2530 2804 2951 3362 971
Size: 66 294 521 678 274 147 411 1751
Fragments arranged by size:
1751 678 521 411 294 274 147 66
BseYI C'CCAG_C
Cuts at: 601 1044 1567 2325 2902 3398 601
Size: 443 523 758 577 496 1345
Fragments arranged by size:
1345 758 577 523 496 443
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 472
Size: 4142
BsiEI CG_ry'CG
Cuts at: 104 1684 1730 2938 104
Size: 1580 46 1208 1308
Fragments arranged by size:
1580 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 302 509 656 767 1156 1934 2124 2916 302
Size: 207 147 111 389 778 190 792 1528
Fragments arranged by size:
1528 792 778 389 207 190 147 111
BsmBI CGTCTCn'nnnn_
Cuts at: 483 483
Size: 4142
Bsp1286I G_dGCh'C
Cuts at: 217 302 355 509 656 699 767 1156 1767
Size: 85 53 154 147 43 68 389 611
Cuts at: 1767 1860 1934 2124 2186 2916 217
Size: 93 74 190 62 730 1443
Fragments arranged by size:
1443 730 611 389 190 154 147 93
85 74 68 62 53 43
BspEI T'CCGG_A
Cuts at: 971 1037 3362 971
Size: 66 2325 1751
Fragments arranged by size:
2325 1751 66
BspHI T'CATG_A
Cuts at: 3318 3318
Size: 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 494 895 1192 1406 1708 2089 494
Size: 401 297 214 302 381 2547
Fragments arranged by size:
2547 401 381 302 297 214
BsrBI CCG'CTC
Cuts at: 50 210 2434 2488 3972 4137 50
Size: 160 2224 54 1484 165 55
Fragments arranged by size:
2224 1484 165 160 55 54
BsrDI GCAATG_nn'
Cuts at: 2054 2054
Size: 4142
BsrFI r'CCGG_y
Cuts at: 2140 2321 2530 2140
Size: 181 209 3752
Fragments arranged by size:
3752 209 181
BssSI C'ACGA_G
Cuts at: 226 346 2413 2771 226
Size: 120 2067 358 1597
Fragments arranged by size:
2067 1597 358 120
BstAPI GCAn_nnn'nTGC
Cuts at: 1519 1519
Size: 4142
BstBI TT'CG_AA
Cuts at: 2503 2503
Size: 4142
BstXI CCAn_nnnn'nTGG
Cuts at: 381 453 381
Size: 72 4070
BstYI r'GATC_y
Cuts at: 1 155 776 914 968 1052 1167 1634 1657
Size: 154 621 138 54 84 115 467 23
Cuts at: 1657 1992 2238 3250 3336 3348 3694 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 621 467 449 346 335 246 154
138 115 86 84 54 23 12
Bsu36I CC'TnA_GG
Cuts at: 781 781
Size: 4142
BtgI C'CryG_G
Cuts at: 374 374
Size: 4142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2092 2273 2436 2092
Size: 181 163 3798
Fragments arranged by size:
3798 181 163
BtsI GCAGTG_nn'
Cuts at: 302 1385 302
Size: 1083 3059
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 531 566 531
Size: 35 4107
DraI TTT'AAA
Cuts at: 1141 1141
Size: 4142
DraIII CAC_nnn'GTG
Cuts at: 228 228
Size: 4142
DrdI GACnn_nn'nnGTC
Cuts at: 1848 2706 1848
Size: 858 3284
EaeI y'GGCC_r
Cuts at: 371 1119 1727 1901 2292 2319 3664 371
Size: 748 608 174 391 27 1345 849
Fragments arranged by size:
1345 849 748 608 391 174 27
EagI C'GGCC_G
Cuts at: 1727 1727
Size: 4142
EarI CTCTTCn'nnn_
Cuts at: 772 871 2165 2375 772
Size: 99 1294 210 2539
Fragments arranged by size:
2539 1294 210 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2350 2660 2806 3952 2350
Size: 310 146 1146 2540
Fragments arranged by size:
2540 1146 310 146
EcoICRI GAG'CTC
Cuts at: 654 765 1154 654
Size: 111 389 3642
Fragments arranged by size:
3642 389 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 355 753 880 1967 2399 3146 3414 139
Size: 216 398 127 1087 432 747 268 867
Fragments arranged by size:
1087 867 747 432 398 268 216 127
EcoNI CCTnn'n_nnAGG
Cuts at: 936 936
Size: 4142
EcoO109I rG'GnC_Cy
Cuts at: 255 273 465 513 629 696 1070 1281 255
Size: 18 192 48 116 67 374 211 3116
Fragments arranged by size:
3116 374 211 192 116 67 48 18
EcoRI G'AATT_C
Cuts at: 1146 1146
Size: 4142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1023 2161 1023
Size: 1138 3004
FspI TGC'GCA
Cuts at: 1923 1923
Size: 4142
HaeII r_GCGC'y
Cuts at: 476 1576 1824 2846 3825 476
Size: 1100 248 1022 979 793
Fragments arranged by size:
1100 1022 979 793 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 808 840 1984 2014 2016 2046 808
Size: 32 1144 30 2 30 2904
Fragments arranged by size:
2904 1144 32 30 30 2
HincII GTy'rAC
Cuts at: 1181 1181
Size: 4142
KasI G'GCGC_C
Cuts at: 472 1572 1820 472
Size: 1100 248 2794
Fragments arranged by size:
2794 1100 248
KpnI G_GTAC'C
Cuts at: 735 1162 735
Size: 427 3715
MluI A'CGCG_T
Cuts at: 3575 3575
Size: 4142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2813 2997 2813
Size: 184 3958
MscI TGG'CCA
Cuts at: 373 1121 1903 373
Size: 748 782 2612
Fragments arranged by size:
2612 782 748
MslI CAynn'nnrTG
Cuts at: 2131 2258 2131
Size: 127 4015
MspA1I CmG'CkG
Cuts at: 1253 1567 1927 2940 3185 1253
Size: 314 360 1013 245 2210
Fragments arranged by size:
2210 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2323 2323
Size: 4142
NarI GG'CG_CC
Cuts at: 473 1573 1821 473
Size: 1100 248 2794
Fragments arranged by size:
2794 1100 248
NcoI C'CATG_G
Cuts at: 374 374
Size: 4142
NgoMIV G'CCGG_C
Cuts at: 2321 2321
Size: 4142
NspI r_CATG'y
Cuts at: 612 1195 1695 2602 612
Size: 583 500 907 2152
Fragments arranged by size:
2152 907 583 500
PasI CC'CwG_GG
Cuts at: 699 699
Size: 4142
PciI A'CATG_T
Cuts at: 2598 2598
Size: 4142
PflMI CCAn_nnn'nTGG
Cuts at: 456 456
Size: 4142
PmeI GTTT'AAAC
Cuts at: 1141 1141
Size: 4142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 4110
PpuMI rG'GwC_Cy
Cuts at: 465 513 629 465
Size: 48 116 3978
Fragments arranged by size:
3978 116 48
PspOMI G'GGCC_C
Cuts at: 695 695
Size: 4142
PstI C_TGCA'G
Cuts at: 1189 1874 1189
Size: 685 3457
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4142
PvuII CAG'CTG
Cuts at: 1567 1927 1567
Size: 360 3782
RsrII CG'GwC_CG
Cuts at: 2337 2337
Size: 4142
SacI G_AGCT'C
Cuts at: 656 767 1156 656
Size: 111 389 3642
Fragments arranged by size:
3642 389 111
SalI G'TCGA_C
Cuts at: 1179 1179
Size: 4142
SanDI GG'GwC_CC
Cuts at: 629 629
Size: 4142
SapI GCTCTTCn'nnn_
Cuts at: 772 2165 2375 772
Size: 1393 210 2539
Fragments arranged by size:
2539 1393 210
SbfI CC_TGCA'GG
Cuts at: 1189 1189
Size: 4142
ScaI AGT'ACT
Cuts at: 3659 3659
Size: 4142
SfcI C'TryA_G
Cuts at: 33 1185 1870 2863 3054 33
Size: 1152 685 993 191 1121
Fragments arranged by size:
1152 1121 993 685 191
SfoI GGC'GCC
Cuts at: 474 1574 1822 474
Size: 1100 248 2794
Fragments arranged by size:
2794 1100 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
SmaI CCC'GGG
Cuts at: 1164 1164
Size: 4142
SmlI C'TyrA_G
Cuts at: 235 517 1297 2212 2704 2966 235
Size: 282 780 915 492 262 1411
Fragments arranged by size:
1411 915 780 492 282 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4142
SphI G_CATG'C
Cuts at: 1195 1695 1195
Size: 500 3642
StuI AGG'CCT
Cuts at: 898 1109 898
Size: 211 3931
StyI C'CwwG_G
Cuts at: 374 468 1276 1630 374
Size: 94 808 354 2886
Fragments arranged by size:
2886 808 354 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1725 1858 2317 2526 1725
Size: 133 459 209 3341
Fragments arranged by size:
3341 459 209 133
TatI w'GTAC_w
Cuts at: 3657 3657
Size: 4142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3659 3691 3659
Size: 32 4110
Tth111I GACn'n_nGTC
Cuts at: 1939 1939
Size: 4142
XbaI T'CTAG_A
Cuts at: 1173 1173
Size: 4142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 1637 453
Size: 1184 2958
XmaI C'CCGG_G
Cuts at: 1162 1162
Size: 4142
XmnI GAAnn'nnTTC
Cuts at: 880 880
Size: 4142
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BsaXI
BseRI BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI