(Circular) (Six-base) MAPSORT of: pF1KE0674.seq Check: 2969 from: 1 to: 4301 pF1KE0674 4301 bp With 182 enzymes: SgfI * April 24, 2009 13:16 .. AccI GT'mk_AC Cuts at: 1182 1339 1182 Size: 157 4144 Acc65I G'GTAC_C Cuts at: 1317 1317 Size: 4301 AclI AA'CG_TT Cuts at: 4141 4141 Size: 4301 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 285 437 555 975 2126 2558 3305 3573 285 Size: 152 118 420 1151 432 747 268 1013 Fragments arranged by size: 1151 1013 747 432 420 268 152 118 AflIII A'CryG_T Cuts at: 1653 2757 3734 1653 Size: 1104 977 2220 Fragments arranged by size: 2220 1104 977 AgeI A'CCGG_T Cuts at: 2689 2689 Size: 4301 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 706 706 Size: 4301 AlwNI CAG_nnn'CTG Cuts at: 1606 3173 1606 Size: 1567 2734 ApaLI G'TGCA_C Cuts at: 3071 3071 Size: 4301 ApoI r'AATT_y Cuts at: 1305 3654 3910 1305 Size: 2349 256 1696 Fragments arranged by size: 2349 1696 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4301 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4301 AvaI C'yCGr_G Cuts at: 1321 1321 Size: 4301 BamHI G'GATC_C Cuts at: 1326 1326 Size: 4301 BanI G'GyrC_C Cuts at: 1317 1731 1979 2014 1317 Size: 414 248 35 3604 Fragments arranged by size: 3604 414 248 35 BanII G_rGCy'C Cuts at: 1315 2345 1315 Size: 1030 3271 BbeI G_GCGC'C Cuts at: 1735 1983 1735 Size: 248 4053 BbsI GAAGACnn'nnnn_ Cuts at: 347 684 347 Size: 337 3964 BciVI GTATCCnnnnn_n' Cuts at: 1175 2199 2960 4301 1175 Size: 1024 761 1341 1175 Fragments arranged by size: 1341 1175 1024 761 BclI T'GATC_A Cuts at: 1821 1821 Size: 4301 BglII A'GATC_T Cuts at: 1816 3853 1816 Size: 2037 2264 BlpI GC'TnA_GC Cuts at: 1413 1413 Size: 4301 Bme1580I G_kGCm'C Cuts at: 506 1926 2019 3075 506 Size: 1420 93 1056 1732 Fragments arranged by size: 1732 1420 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 959 1043 1163 1513 1594 1929 4073 959 Size: 84 120 350 81 335 2144 1187 Fragments arranged by size: 2144 1187 350 335 120 84 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2668 2668 Size: 4301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 803 887 1302 803 Size: 84 415 3802 Fragments arranged by size: 3802 415 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1477 2356 2848 3146 1477 Size: 879 492 298 2632 Fragments arranged by size: 2632 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4005 4005 Size: 4301 BsaAI yAC'GTr Cuts at: 1163 1654 1163 Size: 491 3810 BsaBI GATnn'nnATC Cuts at: 6 1815 3852 6 Size: 1809 2037 455 Fragments arranged by size: 2037 1809 455 BsaHI Gr'CG_yC Cuts at: 1732 1980 2682 4178 1732 Size: 248 702 1496 1855 Fragments arranged by size: 1855 1496 702 248 BsaWI w'CCGG_w Cuts at: 613 1490 2011 2689 2963 3110 3521 613 Size: 877 521 678 274 147 411 1393 Fragments arranged by size: 1393 877 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 175 175 Size: 4301 BseYI C'CCAG_C Cuts at: 1726 2484 3061 3557 1726 Size: 758 577 496 2470 Fragments arranged by size: 2470 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1843 1889 3097 104 Size: 1739 46 1208 1308 Fragments arranged by size: 1739 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1315 2093 2283 3075 1315 Size: 778 190 792 2541 Fragments arranged by size: 2541 792 778 190 BsmI GAATG_Cn' Cuts at: 671 904 1072 1156 1278 671 Size: 233 168 84 122 3694 Fragments arranged by size: 3694 233 168 122 84 Bsp1286I G_dGCh'C Cuts at: 506 1315 1926 2019 2093 2283 2345 3075 506 Size: 809 611 93 74 190 62 730 1732 Fragments arranged by size: 1732 809 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3521 3521 Size: 4301 BspHI T'CATG_A Cuts at: 3477 3477 Size: 4301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1351 1565 1867 2248 1351 Size: 214 302 381 3404 Fragments arranged by size: 3404 381 302 214 BsrBI CCG'CTC Cuts at: 50 2593 2647 4131 4296 50 Size: 2543 54 1484 165 55 Fragments arranged by size: 2543 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 898 2213 898 Size: 1315 2986 BsrFI r'CCGG_y Cuts at: 2299 2480 2689 2299 Size: 181 209 3911 Fragments arranged by size: 3911 209 181 BssSI C'ACGA_G Cuts at: 2572 2930 2572 Size: 358 3943 BstAPI GCAn_nnn'nTGC Cuts at: 1678 1678 Size: 4301 BstBI TT'CG_AA Cuts at: 675 2662 675 Size: 1987 2314 BstYI r'GATC_y Cuts at: 1 1087 1326 1793 1816 2151 2397 3409 3495 Size: 1086 239 467 23 335 246 1012 86 Cuts at: 3495 3507 3853 1 Size: 12 346 449 Fragments arranged by size: 1086 1012 467 449 346 335 246 239 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2251 2432 2595 2251 Size: 181 163 3957 Fragments arranged by size: 3957 181 163 BtsI GCAGTG_nn' Cuts at: 1234 1544 1234 Size: 310 3991 DraI TTT'AAA Cuts at: 1300 1300 Size: 4301 DrdI GACnn_nn'nnGTC Cuts at: 2007 2865 2007 Size: 858 3443 EaeI y'GGCC_r Cuts at: 1886 2060 2451 2478 3823 1886 Size: 174 391 27 1345 2364 Fragments arranged by size: 2364 1345 391 174 27 EagI C'GGCC_G Cuts at: 1886 1886 Size: 4301 EarI CTCTTCn'nnn_ Cuts at: 2324 2534 2324 Size: 210 4091 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2509 2819 2965 4111 2509 Size: 310 146 1146 2699 Fragments arranged by size: 2699 1146 310 146 EcoICRI GAG'CTC Cuts at: 1313 1313 Size: 4301 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 285 437 555 803 887 975 1302 2126 2558 Size: 152 118 248 84 88 327 824 432 Cuts at: 2558 3305 3573 285 Size: 747 268 1013 Fragments arranged by size: 1013 824 747 432 327 268 248 152 118 88 84 EcoO109I rG'GnC_Cy Cuts at: 117 1193 1440 117 Size: 1076 247 2978 Fragments arranged by size: 2978 1076 247 EcoRI G'AATT_C Cuts at: 1305 1305 Size: 4301 EcoRV GAT'ATC Cuts at: 577 577 Size: 4301 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 585 2320 585 Size: 1735 2566 FspI TGC'GCA Cuts at: 2082 2082 Size: 4301 HaeII r_GCGC'y Cuts at: 1735 1983 3005 3984 1735 Size: 248 1022 979 2052 Fragments arranged by size: 2052 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2143 2173 2175 2205 2143 Size: 30 2 30 4239 Fragments arranged by size: 4239 30 30 2 HincII GTy'rAC Cuts at: 553 1340 553 Size: 787 3514 HindIII A'AGCT_T Cuts at: 407 407 Size: 4301 KasI G'GCGC_C Cuts at: 1731 1979 1731 Size: 248 4053 KpnI G_GTAC'C Cuts at: 1321 1321 Size: 4301 MluI A'CGCG_T Cuts at: 3734 3734 Size: 4301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2972 3156 2972 Size: 184 4117 MscI TGG'CCA Cuts at: 2062 2062 Size: 4301 MslI CAynn'nnrTG Cuts at: 546 688 966 2290 2417 546 Size: 142 278 1324 127 2430 Fragments arranged by size: 2430 1324 278 142 127 MspA1I CmG'CkG Cuts at: 1412 1726 2086 3099 3344 1412 Size: 314 360 1013 245 2369 Fragments arranged by size: 2369 1013 360 314 245 NaeI GCC'GGC Cuts at: 2482 2482 Size: 4301 NarI GG'CG_CC Cuts at: 1732 1980 1732 Size: 248 4053 NdeI CA'TA_TG Cuts at: 963 963 Size: 4301 NgoMIV G'CCGG_C Cuts at: 2480 2480 Size: 4301 NspI r_CATG'y Cuts at: 1354 1854 2761 1354 Size: 500 907 2894 Fragments arranged by size: 2894 907 500 PciI A'CATG_T Cuts at: 2757 2757 Size: 4301 PfoI T'CCnGG_A Cuts at: 395 395 Size: 4301 PmeI GTTT'AAAC Cuts at: 1300 1300 Size: 4301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 220 252 3440 3472 220 Size: 32 3188 32 1049 Fragments arranged by size: 3188 1049 32 32 PpuMI rG'GwC_Cy Cuts at: 117 1193 117 Size: 1076 3225 PsiI TTA'TAA Cuts at: 713 1250 713 Size: 537 3764 PstI C_TGCA'G Cuts at: 1348 2033 1348 Size: 685 3616 PvuI CG_AT'CG Cuts at: 104 104 Size: 4301 PvuII CAG'CTG Cuts at: 1726 2086 1726 Size: 360 3941 RsrII CG'GwC_CG Cuts at: 2496 2496 Size: 4301 SacI G_AGCT'C Cuts at: 1315 1315 Size: 4301 SalI G'TCGA_C Cuts at: 1338 1338 Size: 4301 SapI GCTCTTCn'nnn_ Cuts at: 2324 2534 2324 Size: 210 4091 SbfI CC_TGCA'GG Cuts at: 1348 1348 Size: 4301 ScaI AGT'ACT Cuts at: 3818 3818 Size: 4301 SfcI C'TryA_G Cuts at: 33 198 239 1344 2029 3022 3213 33 Size: 165 41 1105 685 993 191 1121 Fragments arranged by size: 1121 1105 993 685 191 165 41 SfoI GGC'GCC Cuts at: 1733 1981 1733 Size: 248 4053 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4301 SmaI CCC'GGG Cuts at: 1323 1323 Size: 4301 SmlI C'TyrA_G Cuts at: 1456 2371 2863 3125 1456 Size: 915 492 262 2632 Fragments arranged by size: 2632 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4301 SphI G_CATG'C Cuts at: 1354 1854 1354 Size: 500 3801 StyI C'CwwG_G Cuts at: 1435 1789 1435 Size: 354 3947 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 169 1884 2017 2476 2685 169 Size: 1715 133 459 209 1785 Fragments arranged by size: 1785 1715 459 209 133 TatI w'GTAC_w Cuts at: 3816 3816 Size: 4301 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3818 3850 3818 Size: 32 4269 Tth111I GACn'n_nGTC Cuts at: 2098 2098 Size: 4301 XbaI T'CTAG_A Cuts at: 1332 1332 Size: 4301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1796 1796 Size: 4301 XmaI C'CCGG_G Cuts at: 1321 1321 Size: 4301 XmnI GAAnn'nnTTC Cuts at: 568 904 568 Size: 336 3965 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI NcoI NheI NotI NruI NsiI PacI PasI PflMI PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI