Restriction map for
pF1KE0676
(Circular) (Six-base) MAPSORT of: pF1KE0676.seq Check: 3014 from: 1 to: 4301
pF1KE0676 4301 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 459 1339 459
Size: 880 3421
Acc65I G'GTAC_C
Cuts at: 1317 1317
Size: 4301
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 442 505 2126 2558 3305 3573 266
Size: 176 63 1621 432 747 268 994
Fragments arranged by size:
1621 994 747 432 268 176 63
AflII C'TTAA_G
Cuts at: 582 582
Size: 4301
AflIII A'CryG_T
Cuts at: 1653 2757 3734 1653
Size: 1104 977 2220
Fragments arranged by size:
2220 1104 977
AgeI A'CCGG_T
Cuts at: 2689 2689
Size: 4301
AleI CACnn'nnGTG
Cuts at: 809 809
Size: 4301
AlwNI CAG_nnn'CTG
Cuts at: 1606 3173 1606
Size: 1567 2734
ApaLI G'TGCA_C
Cuts at: 3071 3071
Size: 4301
ApoI r'AATT_y
Cuts at: 276 392 587 784 1062 1305 3654 3910 276
Size: 116 195 197 278 243 2349 256 667
Fragments arranged by size:
2349 667 278 256 243 197 195 116
AseI AT'TA_AT
Cuts at: 20 20
Size: 4301
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 1321 1321
Size: 4301
BamHI G'GATC_C
Cuts at: 1326 1326
Size: 4301
BanI G'GyrC_C
Cuts at: 1317 1731 1979 2014 1317
Size: 414 248 35 3604
Fragments arranged by size:
3604 414 248 35
BanII G_rGCy'C
Cuts at: 148 1315 2345 148
Size: 1167 1030 2104
Fragments arranged by size:
2104 1167 1030
BbeI G_GCGC'C
Cuts at: 1735 1983 1735
Size: 248 4053
BbsI GAAGACnn'nnnn_
Cuts at: 144 758 144
Size: 614 3687
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4267
BciVI GTATCCnnnnn_n'
Cuts at: 2199 2960 4301 2199
Size: 761 1341 2199
Fragments arranged by size:
2199 1341 761
BclI T'GATC_A
Cuts at: 1821 1821
Size: 4301
BglI GCCn_nnn'nGGC
Cuts at: 245 245
Size: 4301
BglII A'GATC_T
Cuts at: 1816 3853 1816
Size: 2037 2264
BlpI GC'TnA_GC
Cuts at: 577 1413 577
Size: 836 3465
Bme1580I G_kGCm'C
Cuts at: 1926 2019 3075 1926
Size: 93 1056 3152
Fragments arranged by size:
3152 1056 93
BmgBI CAC'GTC
Cuts at: 829 829
Size: 4301
BmrI ACTGGGnnnn_n'
Cuts at: 323 1513 1594 1929 4073 323
Size: 1190 81 335 2144 551
Fragments arranged by size:
2144 1190 551 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 113 1477 2356 2848 3146 113
Size: 1364 879 492 298 1268
Fragments arranged by size:
1364 1268 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4005 4005
Size: 4301
BsaAI yAC'GTr
Cuts at: 1069 1654 1069
Size: 585 3716
BsaBI GATnn'nnATC
Cuts at: 6 1815 3852 6
Size: 1809 2037 455
Fragments arranged by size:
2037 1809 455
BsaHI Gr'CG_yC
Cuts at: 1732 1980 2682 4178 1732
Size: 248 702 1496 1855
Fragments arranged by size:
1855 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1490 2011 2689 2963 3110 3521 1490
Size: 521 678 274 147 411 2270
Fragments arranged by size:
2270 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1264 1264
Size: 4301
BseYI C'CCAG_C
Cuts at: 1726 2484 3061 3557 1726
Size: 758 577 496 2470
Fragments arranged by size:
2470 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1843 1889 3097 104
Size: 1739 46 1208 1308
Fragments arranged by size:
1739 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 148 1315 2093 2283 3075 148
Size: 1167 778 190 792 1374
Fragments arranged by size:
1374 1167 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 148 1315 1926 2019 2093 2283 2345 3075 148
Size: 1167 611 93 74 190 62 730 1374
Fragments arranged by size:
1374 1167 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3521 3521
Size: 4301
BspHI T'CATG_A
Cuts at: 3477 3477
Size: 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1351 1565 1867 2248 1351
Size: 214 302 381 3404
Fragments arranged by size:
3404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2593 2647 4131 4296 50
Size: 2543 54 1484 165 55
Fragments arranged by size:
2543 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2213 2213
Size: 4301
BsrFI r'CCGG_y
Cuts at: 2299 2480 2689 2299
Size: 181 209 3911
Fragments arranged by size:
3911 209 181
BssSI C'ACGA_G
Cuts at: 113 613 789 2572 2930 113
Size: 500 176 1783 358 1484
Fragments arranged by size:
1783 1484 500 358 176
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 1678
Size: 4301
BstBI TT'CG_AA
Cuts at: 2662 2662
Size: 4301
BstXI CCAn_nnnn'nTGG
Cuts at: 1014 1014
Size: 4301
BstYI r'GATC_y
Cuts at: 1 906 1326 1793 1816 2151 2397 3409 3495
Size: 905 420 467 23 335 246 1012 86
Cuts at: 3495 3507 3853 1
Size: 12 346 449
Fragments arranged by size:
1012 905 467 449 420 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2251 2432 2595 2251
Size: 181 163 3957
Fragments arranged by size:
3957 181 163
BtsI GCAGTG_nn'
Cuts at: 303 1544 303
Size: 1241 3060
ClaI AT'CG_AT
Cuts at: 797 797
Size: 4301
DraI TTT'AAA
Cuts at: 1300 1300
Size: 4301
DraIII CAC_nnn'GTG
Cuts at: 310 310
Size: 4301
DrdI GACnn_nn'nnGTC
Cuts at: 2007 2865 2007
Size: 858 3443
EaeI y'GGCC_r
Cuts at: 237 876 1886 2060 2451 2478 3823 237
Size: 639 1010 174 391 27 1345 715
Fragments arranged by size:
1345 1010 715 639 391 174 27
EagI C'GGCC_G
Cuts at: 1886 1886
Size: 4301
EarI CTCTTCn'nnn_
Cuts at: 2324 2534 2324
Size: 210 4091
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2509 2819 2965 4111 2509
Size: 310 146 1146 2699
Fragments arranged by size:
2699 1146 310 146
EcoICRI GAG'CTC
Cuts at: 146 1313 146
Size: 1167 3134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 442 505 2126 2558 3305 3573 266
Size: 176 63 1621 432 747 268 994
Fragments arranged by size:
1621 994 747 432 268 176 63
EcoO109I rG'GnC_Cy
Cuts at: 1440 1440
Size: 4301
EcoRI G'AATT_C
Cuts at: 276 587 784 1305 276
Size: 311 197 521 3272
Fragments arranged by size:
3272 521 311 197
EcoRV GAT'ATC
Cuts at: 639 639
Size: 4301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 4301
FspI TGC'GCA
Cuts at: 2082 2082
Size: 4301
HaeII r_GCGC'y
Cuts at: 1735 1983 3005 3984 1735
Size: 248 1022 979 2052
Fragments arranged by size:
2052 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 486 518 2143 2173 2175 2205 486
Size: 32 1625 30 2 30 2582
Fragments arranged by size:
2582 1625 32 30 30 2
HincII GTy'rAC
Cuts at: 1340 1340
Size: 4301
HindIII A'AGCT_T
Cuts at: 579 579
Size: 4301
KasI G'GCGC_C
Cuts at: 1731 1979 1731
Size: 248 4053
KpnI G_GTAC'C
Cuts at: 1321 1321
Size: 4301
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 162 2972 3156 162
Size: 2810 184 1307
Fragments arranged by size:
2810 1307 184
MscI TGG'CCA
Cuts at: 239 878 2062 239
Size: 639 1184 2478
Fragments arranged by size:
2478 1184 639
MslI CAynn'nnrTG
Cuts at: 617 623 809 2290 2417 617
Size: 6 186 1481 127 2501
Fragments arranged by size:
2501 1481 186 127 6
MspA1I CmG'CkG
Cuts at: 1412 1726 2086 3099 3344 1412
Size: 314 360 1013 245 2369
Fragments arranged by size:
2369 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2482 2482
Size: 4301
NarI GG'CG_CC
Cuts at: 1732 1980 1732
Size: 248 4053
NgoMIV G'CCGG_C
Cuts at: 2480 2480
Size: 4301
NspI r_CATG'y
Cuts at: 1354 1854 2761 1354
Size: 500 907 2894
Fragments arranged by size:
2894 907 500
PciI A'CATG_T
Cuts at: 2757 2757
Size: 4301
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 4269
PstI C_TGCA'G
Cuts at: 1348 2033 1348
Size: 685 3616
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4301
PvuII CAG'CTG
Cuts at: 1726 2086 1726
Size: 360 3941
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 148 1315 148
Size: 1167 3134
SalI G'TCGA_C
Cuts at: 1338 1338
Size: 4301
SapI GCTCTTCn'nnn_
Cuts at: 2324 2534 2324
Size: 210 4091
SbfI CC_TGCA'GG
Cuts at: 1348 1348
Size: 4301
ScaI AGT'ACT
Cuts at: 3818 3818
Size: 4301
SexAI A'CCwGG_T
Cuts at: 680 993 1209 680
Size: 313 216 3772
Fragments arranged by size:
3772 313 216
SfcI C'TryA_G
Cuts at: 33 1097 1344 2029 3022 3213 33
Size: 1064 247 685 993 191 1121
Fragments arranged by size:
1121 1064 993 685 247 191
SfoI GGC'GCC
Cuts at: 1733 1981 1733
Size: 248 4053
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SmaI CCC'GGG
Cuts at: 1323 1323
Size: 4301
SmlI C'TyrA_G
Cuts at: 128 582 1456 2371 2863 3125 128
Size: 454 874 915 492 262 1304
Fragments arranged by size:
1304 915 874 492 454 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 1354 1854 1354
Size: 500 3801
SspI AAT'ATT
Cuts at: 951 951
Size: 4301
StuI AGG'CCT
Cuts at: 646 646
Size: 4301
StyI C'CwwG_G
Cuts at: 240 886 1435 1789 240
Size: 646 549 354 2752
Fragments arranged by size:
2752 646 549 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 421 1884 2017 2476 2685 421
Size: 1463 133 459 209 2037
Fragments arranged by size:
2037 1463 459 209 133
TatI w'GTAC_w
Cuts at: 686 3816 686
Size: 3130 1171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4269
Tth111I GACn'n_nGTC
Cuts at: 2098 2098
Size: 4301
XbaI T'CTAG_A
Cuts at: 1332 1332
Size: 4301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 247 871 1796 247
Size: 624 925 2752
Fragments arranged by size:
2752 925 624
XmaI C'CCGG_G
Cuts at: 1321 1321
Size: 4301
XmnI GAAnn'nnTTC
Cuts at: 608 731 608
Size: 123 4178
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmtI BpmI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtgI CspCI EcoNI FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI