Restriction map for
pF1KE0677
(Circular) (Six-base) MAPSORT of: pF1KE0677.seq Check: 3809 from: 1 to: 3902
pF1KE0677 3902 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 940 940
Size: 3902
Acc65I G'GTAC_C
Cuts at: 918 918
Size: 3902
AclI AA'CG_TT
Cuts at: 3742 3742
Size: 3902
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 1727 2159 2906 3174 217
Size: 1510 432 747 268 945
Fragments arranged by size:
1510 945 747 432 268
AflIII A'CryG_T
Cuts at: 1254 2358 3335 1254
Size: 1104 977 1821
Fragments arranged by size:
1821 1104 977
AgeI A'CCGG_T
Cuts at: 2290 2290
Size: 3902
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 522 554 522
Size: 32 3870
AlwNI CAG_nnn'CTG
Cuts at: 240 522 763 1207 2774 240
Size: 282 241 444 1567 1368
Fragments arranged by size:
1567 1368 444 282 241
ApaI G_GGCC'C
Cuts at: 640 640
Size: 3902
ApaLI G'TGCA_C
Cuts at: 2672 2672
Size: 3902
ApoI r'AATT_y
Cuts at: 906 3255 3511 906
Size: 2349 256 1297
Fragments arranged by size:
2349 1297 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3902
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3902
AvaI C'yCGr_G
Cuts at: 250 922 250
Size: 672 3230
BamHI G'GATC_C
Cuts at: 927 927
Size: 3902
BanI G'GyrC_C
Cuts at: 828 918 1332 1580 1615 828
Size: 90 414 248 35 3115
Fragments arranged by size:
3115 414 248 90 35
BanII G_rGCy'C
Cuts at: 305 422 640 916 1946 305
Size: 117 218 276 1030 2261
Fragments arranged by size:
2261 1030 276 218 117
BbeI G_GCGC'C
Cuts at: 832 1336 1584 832
Size: 504 248 3150
Fragments arranged by size:
3150 504 248
BbsI GAAGACnn'nnnn_
Cuts at: 279 382 279
Size: 103 3799
BciVI GTATCCnnnnn_n'
Cuts at: 1800 2561 3902 1800
Size: 761 1341 1800
Fragments arranged by size:
1800 1341 761
BclI T'GATC_A
Cuts at: 1422 1422
Size: 3902
BglI GCCn_nnn'nGGC
Cuts at: 724 826 724
Size: 102 3800
BglII A'GATC_T
Cuts at: 1417 3454 1417
Size: 2037 1865
BlpI GC'TnA_GC
Cuts at: 1014 1014
Size: 3902
Bme1580I G_kGCm'C
Cuts at: 145 640 1527 1620 2676 145
Size: 495 887 93 1056 1371
Fragments arranged by size:
1371 1056 887 495 93
BmrI ACTGGGnnnn_n'
Cuts at: 1114 1195 1530 3674 1114
Size: 81 335 2144 1342
Fragments arranged by size:
2144 1342 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 319 2269 319
Size: 1950 1952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 526 428
Size: 98 3804
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1957 2449 2747 1078
Size: 879 492 298 2233
Fragments arranged by size:
2233 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3606 3606
Size: 3902
BsaAI yAC'GTr
Cuts at: 1255 1255
Size: 3902
BsaBI GATnn'nnATC
Cuts at: 6 1416 3453 6
Size: 1410 2037 455
Fragments arranged by size:
2037 1410 455
BsaHI Gr'CG_yC
Cuts at: 829 1333 1581 2283 3779 829
Size: 504 248 702 1496 952
Fragments arranged by size:
1496 952 702 504 248
BsaWI w'CCGG_w
Cuts at: 215 1091 1612 2290 2564 2711 3122 215
Size: 876 521 678 274 147 411 995
Fragments arranged by size:
995 876 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 426 445 456 475 426
Size: 19 11 19 3853
Fragments arranged by size:
3853 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 431 431
Size: 3902
BseYI C'CCAG_C
Cuts at: 512 1327 2085 2662 3158 512
Size: 815 758 577 496 1256
Fragments arranged by size:
1256 815 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 358
Size: 3902
BsiEI CG_ry'CG
Cuts at: 104 1444 1490 2698 104
Size: 1340 46 1208 1308
Fragments arranged by size:
1340 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 305 649 755 916 1694 1884 2676 305
Size: 344 106 161 778 190 792 1531
Fragments arranged by size:
1531 792 778 344 190 161 106
Bsp1286I G_dGCh'C
Cuts at: 145 305 422 640 649 755 916 1527 1620
Size: 160 117 218 9 106 161 611 93
Cuts at: 1620 1694 1884 1946 2676 145
Size: 74 190 62 730 1371
Fragments arranged by size:
1371 730 611 218 190 161 160 117
106 93 74 62 9
BspEI T'CCGG_A
Cuts at: 3122 3122
Size: 3902
BspHI T'CATG_A
Cuts at: 3078 3078
Size: 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 330 952 1166 1468 1849 330
Size: 622 214 302 381 2383
Fragments arranged by size:
2383 622 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2194 2248 3732 3897 50
Size: 2144 54 1484 165 55
Fragments arranged by size:
2144 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1814 1814
Size: 3902
BsrFI r'CCGG_y
Cuts at: 825 1900 2081 2290 825
Size: 1075 181 209 2437
Fragments arranged by size:
2437 1075 209 181
BssSI C'ACGA_G
Cuts at: 2173 2531 2173
Size: 358 3544
BstAPI GCAn_nnn'nTGC
Cuts at: 1279 1279
Size: 3902
BstBI TT'CG_AA
Cuts at: 2263 2263
Size: 3902
BstYI r'GATC_y
Cuts at: 1 927 1394 1417 1752 1998 3010 3096 3108
Size: 926 467 23 335 246 1012 86 12
Cuts at: 3108 3454 1
Size: 346 449
Fragments arranged by size:
1012 926 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 422 668 422
Size: 246 3656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1852 2033 2196 1852
Size: 181 163 3558
Fragments arranged by size:
3558 181 163
BtsI GCAGTG_nn'
Cuts at: 120 143 1145 120
Size: 23 1002 2877
Fragments arranged by size:
2877 1002 23
DraI TTT'AAA
Cuts at: 901 901
Size: 3902
DrdI GACnn_nn'nnGTC
Cuts at: 1608 2466 1608
Size: 858 3044
EaeI y'GGCC_r
Cuts at: 590 716 725 1487 1661 2052 2079 3424 590
Size: 126 9 762 174 391 27 1345 1068
Fragments arranged by size:
1345 1068 762 391 174 126 27 9
EagI C'GGCC_G
Cuts at: 1487 1487
Size: 3902
EarI CTCTTCn'nnn_
Cuts at: 171 236 293 883 1925 2135 171
Size: 65 57 590 1042 210 1938
Fragments arranged by size:
1938 1042 590 210 65 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2110 2420 2566 3712 2110
Size: 310 146 1146 2300
Fragments arranged by size:
2300 1146 310 146
EcoICRI GAG'CTC
Cuts at: 303 914 303
Size: 611 3291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 428 526 1727 2159 2906 3174 217
Size: 211 98 1201 432 747 268 945
Fragments arranged by size:
1201 945 747 432 268 211 98
EcoO109I rG'GnC_Cy
Cuts at: 389 538 547 636 1041 389
Size: 149 9 89 405 3250
Fragments arranged by size:
3250 405 149 89 9
EcoRI G'AATT_C
Cuts at: 906 906
Size: 3902
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1921 1921
Size: 3902
FspI TGC'GCA
Cuts at: 736 1683 736
Size: 947 2955
HaeII r_GCGC'y
Cuts at: 210 505 832 1336 1584 2606 3585 210
Size: 295 327 504 248 1022 979 527
Fragments arranged by size:
1022 979 527 504 327 295 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 213 245 426 458 1744 1774 1776 1806 213
Size: 32 181 32 1286 30 2 30 2309
Fragments arranged by size:
2309 1286 181 32 32 30 30 2
HincII GTy'rAC
Cuts at: 941 941
Size: 3902
KasI G'GCGC_C
Cuts at: 828 1332 1580 828
Size: 504 248 3150
Fragments arranged by size:
3150 504 248
KpnI G_GTAC'C
Cuts at: 922 922
Size: 3902
MluI A'CGCG_T
Cuts at: 3335 3335
Size: 3902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2573 2757 2573
Size: 184 3718
MscI TGG'CCA
Cuts at: 727 1663 727
Size: 936 2966
MslI CAynn'nnrTG
Cuts at: 1891 2018 1891
Size: 127 3775
MspA1I CmG'CkG
Cuts at: 240 1013 1327 1687 2700 2945 240
Size: 773 314 360 1013 245 1197
Fragments arranged by size:
1197 1013 773 360 314 245
NaeI GCC'GGC
Cuts at: 827 2083 827
Size: 1256 2646
NarI GG'CG_CC
Cuts at: 829 1333 1581 829
Size: 504 248 3150
Fragments arranged by size:
3150 504 248
NcoI C'CATG_G
Cuts at: 422 668 422
Size: 246 3656
NgoMIV G'CCGG_C
Cuts at: 825 2081 825
Size: 1256 2646
NspI r_CATG'y
Cuts at: 955 1455 2362 955
Size: 500 907 2495
Fragments arranged by size:
2495 907 500
PasI CC'CwG_GG
Cuts at: 472 472
Size: 3902
PciI A'CATG_T
Cuts at: 2358 2358
Size: 3902
PmeI GTTT'AAAC
Cuts at: 901 901
Size: 3902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3041 3073 3041
Size: 32 3870
PpuMI rG'GwC_Cy
Cuts at: 538 538
Size: 3902
PspOMI G'GGCC_C
Cuts at: 636 636
Size: 3902
PstI C_TGCA'G
Cuts at: 546 773 949 1634 546
Size: 227 176 685 2814
Fragments arranged by size:
2814 685 227 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3902
PvuII CAG'CTG
Cuts at: 240 1327 1687 240
Size: 1087 360 2455
Fragments arranged by size:
2455 1087 360
RsrII CG'GwC_CG
Cuts at: 2097 2097
Size: 3902
SacI G_AGCT'C
Cuts at: 305 916 305
Size: 611 3291
SalI G'TCGA_C
Cuts at: 939 939
Size: 3902
SapI GCTCTTCn'nnn_
Cuts at: 293 1925 2135 293
Size: 1632 210 2060
Fragments arranged by size:
2060 1632 210
SbfI CC_TGCA'GG
Cuts at: 546 949 546
Size: 403 3499
ScaI AGT'ACT
Cuts at: 3419 3419
Size: 3902
SfcI C'TryA_G
Cuts at: 33 542 769 945 1630 2623 2814 33
Size: 509 227 176 685 993 191 1121
Fragments arranged by size:
1121 993 685 509 227 191 176
SfiI GGCCn_nnn'nGGCC
Cuts at: 724 724
Size: 3902
SfoI GGC'GCC
Cuts at: 830 1334 1582 830
Size: 504 248 3150
Fragments arranged by size:
3150 504 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3902
SmaI CCC'GGG
Cuts at: 924 924
Size: 3902
SmlI C'TyrA_G
Cuts at: 250 1057 1972 2464 2726 250
Size: 807 915 492 262 1426
Fragments arranged by size:
1426 915 807 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3902
SphI G_CATG'C
Cuts at: 955 1455 955
Size: 500 3402
StuI AGG'CCT
Cuts at: 607 607
Size: 3902
StyI C'CwwG_G
Cuts at: 413 422 668 866 1036 1390 413
Size: 9 246 198 170 354 2925
Fragments arranged by size:
2925 354 246 198 170 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1485 1618 2077 2286 1485
Size: 133 459 209 3101
Fragments arranged by size:
3101 459 209 133
TatI w'GTAC_w
Cuts at: 3417 3417
Size: 3902
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 3870
Tth111I GACn'n_nGTC
Cuts at: 1699 1699
Size: 3902
XbaI T'CTAG_A
Cuts at: 933 933
Size: 3902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 420 1397 420
Size: 977 2925
XhoI C'TCGA_G
Cuts at: 250 250
Size: 3902
XmaI C'CCGG_G
Cuts at: 922 922
Size: 3902
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI