Restriction map for
pF1KE0680
(Circular) (Six-base) MAPSORT of: pF1KE0680.seq Check: 6063 from: 1 to: 4310
pF1KE0680 4310 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1348 1348
Size: 4310
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 4150 4150
Size: 4310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 2135 2567 3314 3582 928
Size: 1207 432 747 268 1656
Fragments arranged by size:
1656 1207 747 432 268
AfeI AGC'GCT
Cuts at: 742 742
Size: 4310
AflIII A'CryG_T
Cuts at: 656 1662 2766 3743 656
Size: 1006 1104 977 1223
Fragments arranged by size:
1223 1104 1006 977
AgeI A'CCGG_T
Cuts at: 2698 2698
Size: 4310
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1067 1099 1067
Size: 32 4278
AlwNI CAG_nnn'CTG
Cuts at: 192 213 1615 3182 192
Size: 21 1402 1567 1320
Fragments arranged by size:
1567 1402 1320 21
ApaLI G'TGCA_C
Cuts at: 3080 3080
Size: 4310
ApoI r'AATT_y
Cuts at: 1314 3663 3919 1314
Size: 2349 256 1705
Fragments arranged by size:
2349 1705 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 385 1004 1330 385
Size: 619 326 3365
Fragments arranged by size:
3365 619 326
BamHI G'GATC_C
Cuts at: 344 1335 344
Size: 991 3319
BanI G'GyrC_C
Cuts at: 1326 1740 1988 2023 1326
Size: 414 248 35 3613
Fragments arranged by size:
3613 414 248 35
BanII G_rGCy'C
Cuts at: 227 833 1004 1324 2354 227
Size: 606 171 320 1030 2183
Fragments arranged by size:
2183 1030 606 320 171
BbeI G_GCGC'C
Cuts at: 1744 1992 1744
Size: 248 4062
BbsI GAAGACnn'nnnn_
Cuts at: 934 934
Size: 4310
BciVI GTATCCnnnnn_n'
Cuts at: 2208 2969 4310 2208
Size: 761 1341 2208
Fragments arranged by size:
2208 1341 761
BclI T'GATC_A
Cuts at: 1830 1830
Size: 4310
BglII A'GATC_T
Cuts at: 1825 3862 1825
Size: 2037 2273
BlpI GC'TnA_GC
Cuts at: 1422 1422
Size: 4310
Bme1580I G_kGCm'C
Cuts at: 1935 2028 3084 1935
Size: 93 1056 3161
Fragments arranged by size:
3161 1056 93
BmgBI CAC'GTC
Cuts at: 932 932
Size: 4310
BmrI ACTGGGnnnn_n'
Cuts at: 1522 1603 1938 4082 1522
Size: 81 335 2144 1750
Fragments arranged by size:
2144 1750 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 4310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 277 757 1042 184
Size: 93 480 285 3452
Fragments arranged by size:
3452 480 285 93
Bpu10I CC'TnA_GC
Cuts at: 799 799
Size: 4310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 2365 2857 3155 1486
Size: 879 492 298 2641
Fragments arranged by size:
2641 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4014 4014
Size: 4310
BsaAI yAC'GTr
Cuts at: 659 1663 659
Size: 1004 3306
BsaBI GATnn'nnATC
Cuts at: 6 1824 3861 6
Size: 1818 2037 455
Fragments arranged by size:
2037 1818 455
BsaHI Gr'CG_yC
Cuts at: 119 1741 1989 2691 4187 119
Size: 1622 248 702 1496 242
Fragments arranged by size:
1622 1496 702 248 242
BsaWI w'CCGG_w
Cuts at: 144 293 590 1166 1499 2020 2698 2972 3119
Size: 149 297 576 333 521 678 274 147
Cuts at: 3119 3530 144
Size: 411 924
Fragments arranged by size:
924 678 576 521 411 333 297 274
149 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 235 403 805 1204 163
Size: 72 168 402 399 3269
Fragments arranged by size:
3269 402 399 168 72
BseYI C'CCAG_C
Cuts at: 611 912 1735 2493 3070 3566 611
Size: 301 823 758 577 496 1355
Fragments arranged by size:
1355 823 758 577 496 301
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 550
Size: 4310
BsiEI CG_ry'CG
Cuts at: 104 1137 1852 1898 3106 104
Size: 1033 715 46 1208 1308
Fragments arranged by size:
1308 1208 1033 715 46
BsiHKAI G_wGCw'C
Cuts at: 227 485 1324 2102 2292 3084 227
Size: 258 839 778 190 792 1453
Fragments arranged by size:
1453 839 792 778 258 190
Bsp1286I G_dGCh'C
Cuts at: 227 485 833 1004 1324 1935 2028 2102 2292
Size: 258 348 171 320 611 93 74 190
Cuts at: 2292 2354 3084 227
Size: 62 730 1453
Fragments arranged by size:
1453 730 611 348 320 258 190 171
93 74 62
BspEI T'CCGG_A
Cuts at: 144 3530 144
Size: 3386 924
BspHI T'CATG_A
Cuts at: 3486 3486
Size: 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 791 1360 1574 1876 2257 791
Size: 569 214 302 381 2844
Fragments arranged by size:
2844 569 381 302 214
BsrBI CCG'CTC
Cuts at: 50 153 2602 2656 4140 4305 50
Size: 103 2449 54 1484 165 55
Fragments arranged by size:
2449 1484 165 103 55 54
BsrDI GCAATG_nn'
Cuts at: 311 2222 311
Size: 1911 2399
BsrFI r'CCGG_y
Cuts at: 2308 2489 2698 2308
Size: 181 209 3920
Fragments arranged by size:
3920 209 181
BsrGI T'GTAC_A
Cuts at: 407 407
Size: 4310
BssSI C'ACGA_G
Cuts at: 2581 2939 2581
Size: 358 3952
BstAPI GCAn_nnn'nTGC
Cuts at: 192 1687 192
Size: 1495 2815
BstBI TT'CG_AA
Cuts at: 2671 2671
Size: 4310
BstYI r'GATC_y
Cuts at: 1 344 498 1169 1335 1802 1825 2160 2406
Size: 343 154 671 166 467 23 335 246
Cuts at: 2406 3418 3504 3516 3862 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 671 467 449 346 343 335 246
166 154 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 538 788 845 538
Size: 250 57 4003
Fragments arranged by size:
4003 250 57
BtgI C'CryG_G
Cuts at: 107 989 107
Size: 882 3428
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2260 2441 2604 2260
Size: 181 163 3966
Fragments arranged by size:
3966 181 163
BtsI GCAGTG_nn'
Cuts at: 485 1553 485
Size: 1068 3242
DraI TTT'AAA
Cuts at: 1309 1309
Size: 4310
DraIII CAC_nnn'GTG
Cuts at: 1161 1161
Size: 4310
DrdI GACnn_nn'nnGTC
Cuts at: 2016 2874 2016
Size: 858 3452
EaeI y'GGCC_r
Cuts at: 749 1134 1895 2069 2460 2487 3832 749
Size: 385 761 174 391 27 1345 1227
Fragments arranged by size:
1345 1227 761 391 385 174 27
EagI C'GGCC_G
Cuts at: 1134 1895 1134
Size: 761 3549
EarI CTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 195 1144 2518 2828 2974 4120 195
Size: 949 1374 310 146 1146 385
Fragments arranged by size:
1374 1146 949 385 310 146
EcoICRI GAG'CTC
Cuts at: 225 1322 225
Size: 1097 3213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 277 757 928 1042 2135 2567 3314 3582
Size: 93 480 171 114 1093 432 747 268
Cuts at: 3582 184
Size: 912
Fragments arranged by size:
1093 912 747 480 432 268 171 114
93
EcoNI CCTnn'n_nnAGG
Cuts at: 217 409 787 217
Size: 192 378 3740
Fragments arranged by size:
3740 378 192
EcoO109I rG'GnC_Cy
Cuts at: 534 780 1449 534
Size: 246 669 3395
Fragments arranged by size:
3395 669 246
EcoRI G'AATT_C
Cuts at: 1314 1314
Size: 4310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 4310
FspI TGC'GCA
Cuts at: 2091 2091
Size: 4310
HaeII r_GCGC'y
Cuts at: 213 744 945 1744 1992 3014 3993 213
Size: 531 201 799 248 1022 979 530
Fragments arranged by size:
1022 979 799 531 530 248 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 998 1030 2152 2182 2184 2214 998
Size: 32 1122 30 2 30 3094
Fragments arranged by size:
3094 1122 32 30 30 2
HincII GTy'rAC
Cuts at: 1349 1349
Size: 4310
KasI G'GCGC_C
Cuts at: 1740 1988 1740
Size: 248 4062
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2981 3165 2981
Size: 184 4126
MscI TGG'CCA
Cuts at: 751 2071 751
Size: 1320 2990
MslI CAynn'nnrTG
Cuts at: 2299 2426 2299
Size: 127 4183
MspA1I CmG'CkG
Cuts at: 1421 1735 2095 3108 3353 1421
Size: 314 360 1013 245 2378
Fragments arranged by size:
2378 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 4310
NarI GG'CG_CC
Cuts at: 1741 1989 1741
Size: 248 4062
NcoI C'CATG_G
Cuts at: 107 107
Size: 4310
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 4310
NspI r_CATG'y
Cuts at: 1363 1863 2770 1363
Size: 500 907 2903
Fragments arranged by size:
2903 907 500
PasI CC'CwG_GG
Cuts at: 634 634
Size: 4310
PciI A'CATG_T
Cuts at: 2766 2766
Size: 4310
PflMI CCAn_nnn'nTGG
Cuts at: 354 354
Size: 4310
PfoI T'CCnGG_A
Cuts at: 719 719
Size: 4310
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PmlI CAC'GTG
Cuts at: 659 659
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 4278
PpuMI rG'GwC_Cy
Cuts at: 780 780
Size: 4310
PstI C_TGCA'G
Cuts at: 377 1357 2042 377
Size: 980 685 2645
Fragments arranged by size:
2645 980 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 1735 2095 1735
Size: 360 3950
RsrII CG'GwC_CG
Cuts at: 888 2505 888
Size: 1617 2693
SacI G_AGCT'C
Cuts at: 227 1324 227
Size: 1097 3213
SalI G'TCGA_C
Cuts at: 1347 1347
Size: 4310
SapI GCTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
SbfI CC_TGCA'GG
Cuts at: 1357 1357
Size: 4310
ScaI AGT'ACT
Cuts at: 3827 3827
Size: 4310
SfcI C'TryA_G
Cuts at: 33 373 1353 2038 3031 3222 33
Size: 340 980 685 993 191 1121
Fragments arranged by size:
1121 993 980 685 340 191
SfoI GGC'GCC
Cuts at: 1742 1990 1742
Size: 248 4062
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SmaI CCC'GGG
Cuts at: 1332 1332
Size: 4310
SmlI C'TyrA_G
Cuts at: 1465 2380 2872 3134 1465
Size: 915 492 262 2641
Fragments arranged by size:
2641 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 1363 1863 1363
Size: 500 3810
StuI AGG'CCT
Cuts at: 604 604
Size: 4310
StyI C'CwwG_G
Cuts at: 107 1444 1798 107
Size: 1337 354 2619
Fragments arranged by size:
2619 1337 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1893 2026 2485 2694 1893
Size: 133 459 209 3509
Fragments arranged by size:
3509 459 209 133
TatI w'GTAC_w
Cuts at: 407 983 3825 407
Size: 576 2842 892
Fragments arranged by size:
2842 892 576
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3827 3859 3827
Size: 32 4278
Tth111I GACn'n_nGTC
Cuts at: 118 2107 118
Size: 1989 2321
XbaI T'CTAG_A
Cuts at: 1341 1341
Size: 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 243 1805 243
Size: 1562 2748
XmaI C'CCGG_G
Cuts at: 1330 1330
Size: 4310
XmnI GAAnn'nnTTC
Cuts at: 319 319
Size: 4310
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsaXI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI