Restriction map for
pF1KE0682
(Circular) (Six-base) MAPSORT of: pF1KE0682.seq Check: 3732 from: 1 to: 4280
pF1KE0682 4280 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 884 1318 884
Size: 434 3846
Acc65I G'GTAC_C
Cuts at: 1296 1296
Size: 4280
AclI AA'CG_TT
Cuts at: 4120 4120
Size: 4280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 1216 2105 2537 3284 3552 153
Size: 1063 889 432 747 268 881
Fragments arranged by size:
1063 889 881 747 432 268
AflIII A'CryG_T
Cuts at: 1632 2736 3713 1632
Size: 1104 977 2199
Fragments arranged by size:
2199 1104 977
AgeI A'CCGG_T
Cuts at: 2668 2668
Size: 4280
AlwNI CAG_nnn'CTG
Cuts at: 206 216 385 603 1585 3152 206
Size: 10 169 218 982 1567 1334
Fragments arranged by size:
1567 1334 982 218 169 10
ApaLI G'TGCA_C
Cuts at: 3050 3050
Size: 4280
ApoI r'AATT_y
Cuts at: 1284 3633 3889 1284
Size: 2349 256 1675
Fragments arranged by size:
2349 1675 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4280
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
AvaI C'yCGr_G
Cuts at: 329 1300 329
Size: 971 3309
BamHI G'GATC_C
Cuts at: 1305 1305
Size: 4280
BanI G'GyrC_C
Cuts at: 291 554 1061 1296 1710 1958 1993 291
Size: 263 507 235 414 248 35 2578
Fragments arranged by size:
2578 507 414 263 248 235 35
BanII G_rGCy'C
Cuts at: 440 662 824 1294 2324 440
Size: 222 162 470 1030 2396
Fragments arranged by size:
2396 1030 470 222 162
BbeI G_GCGC'C
Cuts at: 558 1714 1962 558
Size: 1156 248 2876
Fragments arranged by size:
2876 1156 248
BbsI GAAGACnn'nnnn_
Cuts at: 352 352
Size: 4280
BbvCI CC'TCA_GC
Cuts at: 866 866
Size: 4280
BciVI GTATCCnnnnn_n'
Cuts at: 2178 2939 4280 2178
Size: 761 1341 2178
Fragments arranged by size:
2178 1341 761
BclI T'GATC_A
Cuts at: 1800 1800
Size: 4280
BglI GCCn_nnn'nGGC
Cuts at: 250 250
Size: 4280
BglII A'GATC_T
Cuts at: 1795 3832 1795
Size: 2037 2243
BlpI GC'TnA_GC
Cuts at: 631 1392 631
Size: 761 3519
Bme1580I G_kGCm'C
Cuts at: 1064 1905 1998 3054 1064
Size: 841 93 1056 2290
Fragments arranged by size:
2290 1056 841 93
BmrI ACTGGGnnnn_n'
Cuts at: 744 778 1492 1573 1908 4052 744
Size: 34 714 81 335 2144 972
Fragments arranged by size:
2144 972 714 335 81 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 273 2647 273
Size: 2374 1906
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 454 1212 317
Size: 137 758 3385
Fragments arranged by size:
3385 758 137
Bpu10I CC'TnA_GC
Cuts at: 544 680 835 866 544
Size: 136 155 31 3958
Fragments arranged by size:
3958 155 136 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1456 2335 2827 3125 1456
Size: 879 492 298 2611
Fragments arranged by size:
2611 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 319 3984 319
Size: 3665 615
BsaAI yAC'GTr
Cuts at: 561 1633 561
Size: 1072 3208
BsaBI GATnn'nnATC
Cuts at: 6 1794 3831 6
Size: 1788 2037 455
Fragments arranged by size:
2037 1788 455
BsaHI Gr'CG_yC
Cuts at: 555 1711 1959 2661 4157 555
Size: 1156 248 702 1496 678
Fragments arranged by size:
1496 1156 702 678 248
BsaWI w'CCGG_w
Cuts at: 1469 1990 2668 2942 3089 3500 1469
Size: 521 678 274 147 411 2249
Fragments arranged by size:
2249 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1187 1217 1187
Size: 30 4250
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 301 1259 301
Size: 958 3322
BseYI C'CCAG_C
Cuts at: 539 567 827 1009 1124 1705 2463 3040 3536
Size: 28 260 182 115 581 758 577 496
Cuts at: 3536 539
Size: 1283
Fragments arranged by size:
1283 758 581 577 496 260 182 115
28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 595
Size: 4280
BsiEI CG_ry'CG
Cuts at: 104 1822 1868 3076 104
Size: 1718 46 1208 1308
Fragments arranged by size:
1718 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1294 2072 2262 3054 1294
Size: 778 190 792 2520
Fragments arranged by size:
2520 792 778 190
BsmI GAATG_Cn'
Cuts at: 276 1131 276
Size: 855 3425
BsmBI CGTCTCn'nnnn_
Cuts at: 640 640
Size: 4280
Bsp1286I G_dGCh'C
Cuts at: 440 662 824 1064 1294 1905 1998 2072 2262
Size: 222 162 240 230 611 93 74 190
Cuts at: 2262 2324 3054 440
Size: 62 730 1666
Fragments arranged by size:
1666 730 611 240 230 222 190 162
93 74 62
BspEI T'CCGG_A
Cuts at: 3500 3500
Size: 4280
BspHI T'CATG_A
Cuts at: 3456 3456
Size: 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 611 1330 1544 1846 2227 611
Size: 719 214 302 381 2664
Fragments arranged by size:
2664 719 381 302 214
BsrBI CCG'CTC
Cuts at: 50 630 2572 2626 4110 4275 50
Size: 580 1942 54 1484 165 55
Fragments arranged by size:
1942 1484 580 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2192 2192
Size: 4280
BsrFI r'CCGG_y
Cuts at: 2278 2459 2668 2278
Size: 181 209 3890
Fragments arranged by size:
3890 209 181
BssSI C'ACGA_G
Cuts at: 2551 2909 2551
Size: 358 3922
BstAPI GCAn_nnn'nTGC
Cuts at: 206 385 603 1657 206
Size: 179 218 1054 2829
Fragments arranged by size:
2829 1054 218 179
BstBI TT'CG_AA
Cuts at: 2641 2641
Size: 4280
BstEII G'GTnAC_C
Cuts at: 483 483
Size: 4280
BstXI CCAn_nnnn'nTGG
Cuts at: 1190 1190
Size: 4280
BstYI r'GATC_y
Cuts at: 1 1305 1772 1795 2130 2376 3388 3474 3486
Size: 1304 467 23 335 246 1012 86 12
Cuts at: 3486 3832 1
Size: 346 449
Fragments arranged by size:
1304 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 446 700 1271 446
Size: 254 571 3455
Fragments arranged by size:
3455 571 254
BtgI C'CryG_G
Cuts at: 107 396 980 107
Size: 289 584 3407
Fragments arranged by size:
3407 584 289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2230 2411 2574 2230
Size: 181 163 3936
Fragments arranged by size:
3936 181 163
BtsI GCAGTG_nn'
Cuts at: 224 1523 224
Size: 1299 2981
DraI TTT'AAA
Cuts at: 1279 1279
Size: 4280
DraIII CAC_nnn'GTG
Cuts at: 564 564
Size: 4280
DrdI GACnn_nn'nnGTC
Cuts at: 1986 2844 1986
Size: 858 3422
EaeI y'GGCC_r
Cuts at: 1865 2039 2430 2457 3802 1865
Size: 174 391 27 1345 2343
Fragments arranged by size:
2343 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1865 1865
Size: 4280
EarI CTCTTCn'nnn_
Cuts at: 1072 2303 2513 1072
Size: 1231 210 2839
Fragments arranged by size:
2839 1231 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2488 2798 2944 4090 2488
Size: 310 146 1146 2678
Fragments arranged by size:
2678 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1292 1292
Size: 4280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 317 454 1212 1216 2105 2537 3284 3552
Size: 164 137 758 4 889 432 747 268
Cuts at: 3552 153
Size: 881
Fragments arranged by size:
889 881 758 747 432 268 164 137
4
EcoNI CCTnn'n_nnAGG
Cuts at: 299 1097 299
Size: 798 3482
EcoO109I rG'GnC_Cy
Cuts at: 243 600 738 1041 1159 1419 243
Size: 357 138 303 118 260 3104
Fragments arranged by size:
3104 357 303 260 138 118
EcoRI G'AATT_C
Cuts at: 1284 1284
Size: 4280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2299 2299
Size: 4280
FspI TGC'GCA
Cuts at: 2061 2061
Size: 4280
HaeII r_GCGC'y
Cuts at: 558 584 1714 1962 2984 3963 558
Size: 26 1130 248 1022 979 875
Fragments arranged by size:
1130 1022 979 875 248 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1187 1219 2122 2152 2154 2184 1187
Size: 32 903 30 2 30 3283
Fragments arranged by size:
3283 903 32 30 30 2
HincII GTy'rAC
Cuts at: 1319 1319
Size: 4280
KasI G'GCGC_C
Cuts at: 554 1710 1958 554
Size: 1156 248 2876
Fragments arranged by size:
2876 1156 248
KpnI G_GTAC'C
Cuts at: 1300 1300
Size: 4280
MluI A'CGCG_T
Cuts at: 3713 3713
Size: 4280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 284 2951 3135 284
Size: 2667 184 1429
Fragments arranged by size:
2667 1429 184
MscI TGG'CCA
Cuts at: 2041 2041
Size: 4280
MslI CAynn'nnrTG
Cuts at: 190 1021 2269 2396 190
Size: 831 1248 127 2074
Fragments arranged by size:
2074 1248 831 127
MspA1I CmG'CkG
Cuts at: 831 870 1128 1391 1705 2065 3078 3323 831
Size: 39 258 263 314 360 1013 245 1788
Fragments arranged by size:
1788 1013 360 314 263 258 245 39
NaeI GCC'GGC
Cuts at: 2461 2461
Size: 4280
NarI GG'CG_CC
Cuts at: 555 1711 1959 555
Size: 1156 248 2876
Fragments arranged by size:
2876 1156 248
NcoI C'CATG_G
Cuts at: 107 396 980 107
Size: 289 584 3407
Fragments arranged by size:
3407 584 289
NgoMIV G'CCGG_C
Cuts at: 2459 2459
Size: 4280
NspI r_CATG'y
Cuts at: 1333 1833 2740 1333
Size: 500 907 2873
Fragments arranged by size:
2873 907 500
PasI CC'CwG_GG
Cuts at: 997 997
Size: 4280
PciI A'CATG_T
Cuts at: 2736 2736
Size: 4280
PflMI CCAn_nnn'nTGG
Cuts at: 216 216
Size: 4280
PmeI GTTT'AAAC
Cuts at: 1279 1279
Size: 4280
PmlI CAC'GTG
Cuts at: 561 561
Size: 4280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 4248
PpuMI rG'GwC_Cy
Cuts at: 600 738 600
Size: 138 4142
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 205 237 205
Size: 32 4248
PstI C_TGCA'G
Cuts at: 320 371 599 690 1327 2012 320
Size: 51 228 91 637 685 2588
Fragments arranged by size:
2588 685 637 228 91 51
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4280
PvuII CAG'CTG
Cuts at: 831 870 1128 1705 2065 831
Size: 39 258 577 360 3046
Fragments arranged by size:
3046 577 360 258 39
RsrII CG'GwC_CG
Cuts at: 2475 2475
Size: 4280
SacI G_AGCT'C
Cuts at: 1294 1294
Size: 4280
SalI G'TCGA_C
Cuts at: 1317 1317
Size: 4280
SapI GCTCTTCn'nnn_
Cuts at: 1072 2303 2513 1072
Size: 1231 210 2839
Fragments arranged by size:
2839 1231 210
SbfI CC_TGCA'GG
Cuts at: 320 690 1327 320
Size: 370 637 3273
Fragments arranged by size:
3273 637 370
ScaI AGT'ACT
Cuts at: 3797 3797
Size: 4280
SfcI C'TryA_G
Cuts at: 33 316 367 595 686 1323 2008 3001 3192
Size: 283 51 228 91 637 685 993 191
Cuts at: 3192 33
Size: 1121
Fragments arranged by size:
1121 993 685 637 283 228 191 91
51
SfoI GGC'GCC
Cuts at: 556 1712 1960 556
Size: 1156 248 2876
Fragments arranged by size:
2876 1156 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
SmaI CCC'GGG
Cuts at: 331 1302 331
Size: 971 3309
SmlI C'TyrA_G
Cuts at: 1435 2350 2842 3104 1435
Size: 915 492 262 2611
Fragments arranged by size:
2611 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4280
SphI G_CATG'C
Cuts at: 1333 1833 1333
Size: 500 3780
StuI AGG'CCT
Cuts at: 1092 1092
Size: 4280
StyI C'CwwG_G
Cuts at: 107 138 396 980 1056 1260 1414 1768 107
Size: 31 258 584 76 204 154 354 2619
Fragments arranged by size:
2619 584 354 258 204 154 76 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 721 1863 1996 2455 2664 721
Size: 1142 133 459 209 2337
Fragments arranged by size:
2337 1142 459 209 133
TatI w'GTAC_w
Cuts at: 752 3795 752
Size: 3043 1237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3797 3829 3797
Size: 32 4248
Tth111I GACn'n_nGTC
Cuts at: 2077 2077
Size: 4280
XbaI T'CTAG_A
Cuts at: 1219 1311 1219
Size: 92 4188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1114 1775 1114
Size: 661 3619
XmaI C'CCGG_G
Cuts at: 329 1300 329
Size: 971 3309
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsiWI BsrGI BssHII BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI