Restriction map for
pF1KE0685
(Circular) (Six-base) MAPSORT of: pF1KE0685.seq Check: 4612 from: 1 to: 3953
pF1KE0685 3953 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 991 991
Size: 3953
Acc65I G'GTAC_C
Cuts at: 969 969
Size: 3953
AclI AA'CG_TT
Cuts at: 3793 3793
Size: 3953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 1778 2210 2957 3225 532
Size: 1246 432 747 268 1260
Fragments arranged by size:
1260 1246 747 432 268
AflIII A'CryG_T
Cuts at: 629 1305 2409 3386 629
Size: 676 1104 977 1196
Fragments arranged by size:
1196 1104 977 676
AgeI A'CCGG_T
Cuts at: 2341 2341
Size: 3953
AlwNI CAG_nnn'CTG
Cuts at: 592 1258 2825 592
Size: 666 1567 1720
Fragments arranged by size:
1720 1567 666
ApaI G_GGCC'C
Cuts at: 520 927 520
Size: 407 3546
ApaLI G'TGCA_C
Cuts at: 2723 2723
Size: 3953
ApoI r'AATT_y
Cuts at: 934 957 3306 3562 934
Size: 23 2349 256 1325
Fragments arranged by size:
2349 1325 256 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
AvaI C'yCGr_G
Cuts at: 973 973
Size: 3953
BamHI G'GATC_C
Cuts at: 978 978
Size: 3953
BanI G'GyrC_C
Cuts at: 256 969 1383 1631 1666 256
Size: 713 414 248 35 2543
Fragments arranged by size:
2543 713 414 248 35
BanII G_rGCy'C
Cuts at: 406 520 927 967 1997 406
Size: 114 407 40 1030 2362
Fragments arranged by size:
2362 1030 407 114 40
BbeI G_GCGC'C
Cuts at: 1387 1635 1387
Size: 248 3705
BbvCI CC'TCA_GC
Cuts at: 662 662
Size: 3953
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 870 904 870
Size: 34 3919
BciVI GTATCCnnnnn_n'
Cuts at: 166 1851 2612 3953 166
Size: 1685 761 1341 166
Fragments arranged by size:
1685 1341 761 166
BclI T'GATC_A
Cuts at: 1473 1473
Size: 3953
BglI GCCn_nnn'nGGC
Cuts at: 112 170 112
Size: 58 3895
BglII A'GATC_T
Cuts at: 1468 3505 1468
Size: 2037 1916
BlpI GC'TnA_GC
Cuts at: 1065 1065
Size: 3953
Bme1580I G_kGCm'C
Cuts at: 308 520 599 927 1578 1671 2727 308
Size: 212 79 328 651 93 1056 1534
Fragments arranged by size:
1534 1056 651 328 212 93 79
BmrI ACTGGGnnnn_n'
Cuts at: 178 1165 1246 1581 3725 178
Size: 987 81 335 2144 406
Fragments arranged by size:
2144 987 406 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 3953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 805
Size: 3953
Bpu10I CC'TnA_GC
Cuts at: 662 674 662
Size: 12 3941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 2008 2500 2798 1129
Size: 879 492 298 2284
Fragments arranged by size:
2284 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3657 3657
Size: 3953
BsaAI yAC'GTr
Cuts at: 468 1306 468
Size: 838 3115
BsaBI GATnn'nnATC
Cuts at: 6 1467 3504 6
Size: 1461 2037 455
Fragments arranged by size:
2037 1461 455
BsaHI Gr'CG_yC
Cuts at: 1384 1632 2334 3830 1384
Size: 248 702 1496 1507
Fragments arranged by size:
1507 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1142 1663 2341 2615 2762 3173 1142
Size: 521 678 274 147 411 1922
Fragments arranged by size:
1922 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 316 346 403 433 316
Size: 30 57 30 3836
Fragments arranged by size:
3836 57 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 337 700 703 721 907 337
Size: 363 3 18 186 3383
Fragments arranged by size:
3383 363 186 18 3
BseYI C'CCAG_C
Cuts at: 160 713 926 1378 2136 2713 3209 160
Size: 553 213 452 758 577 496 904
Fragments arranged by size:
904 758 577 553 496 452 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 300
Size: 3953
BsiEI CG_ry'CG
Cuts at: 104 943 1495 1541 2749 104
Size: 839 552 46 1208 1308
Fragments arranged by size:
1308 1208 839 552 46
BsiHKAI G_wGCw'C
Cuts at: 240 752 967 1745 1935 2727 240
Size: 512 215 778 190 792 1466
Fragments arranged by size:
1466 792 778 512 215 190
BsiWI C'GTAC_G
Cuts at: 944 944
Size: 3953
BsmI GAATG_Cn'
Cuts at: 560 560
Size: 3953
BsmBI CGTCTCn'nnnn_
Cuts at: 789 789
Size: 3953
Bsp1286I G_dGCh'C
Cuts at: 240 308 406 520 599 752 927 967 1578
Size: 68 98 114 79 153 175 40 611
Cuts at: 1578 1671 1745 1935 1997 2727 240
Size: 93 74 190 62 730 1466
Fragments arranged by size:
1466 730 611 190 175 153 114 98
93 79 74 68 62 40
BspEI T'CCGG_A
Cuts at: 3173 3173
Size: 3953
BspHI T'CATG_A
Cuts at: 3129 3129
Size: 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1003 1217 1519 1900 1003
Size: 214 302 381 3056
Fragments arranged by size:
3056 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2245 2299 3783 3948 50
Size: 2195 54 1484 165 55
Fragments arranged by size:
2195 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1865 1865
Size: 3953
BsrFI r'CCGG_y
Cuts at: 737 1951 2132 2341 737
Size: 1214 181 209 2349
Fragments arranged by size:
2349 1214 209 181
BssHII G'CGCG_C
Cuts at: 115 115
Size: 3953
BssSI C'ACGA_G
Cuts at: 2224 2582 2224
Size: 358 3595
BstAPI GCAn_nnn'nTGC
Cuts at: 1330 1330
Size: 3953
BstBI TT'CG_AA
Cuts at: 2314 2314
Size: 3953
BstEII G'GTnAC_C
Cuts at: 723 723
Size: 3953
BstXI CCAn_nnnn'nTGG
Cuts at: 168 168
Size: 3953
BstYI r'GATC_y
Cuts at: 1 978 1445 1468 1803 2049 3061 3147 3159
Size: 977 467 23 335 246 1012 86 12
Cuts at: 3159 3505 1
Size: 346 449
Fragments arranged by size:
1012 977 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 605 613 605
Size: 8 3945
BtgI C'CryG_G
Cuts at: 107 107
Size: 3953
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1903 2084 2247 1903
Size: 181 163 3609
Fragments arranged by size:
3609 181 163
BtsI GCAGTG_nn'
Cuts at: 1196 1196
Size: 3953
DraI TTT'AAA
Cuts at: 952 952
Size: 3953
DraIII CAC_nnn'GTG
Cuts at: 549 549
Size: 3953
DrdI GACnn_nn'nnGTC
Cuts at: 1659 2517 1659
Size: 858 3095
EaeI y'GGCC_r
Cuts at: 1538 1712 2103 2130 3475 1538
Size: 174 391 27 1345 2016
Fragments arranged by size:
2016 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1538 1538
Size: 3953
EarI CTCTTCn'nnn_
Cuts at: 274 547 1976 2186 274
Size: 273 1429 210 2041
Fragments arranged by size:
2041 1429 273 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2161 2471 2617 3763 2161
Size: 310 146 1146 2351
Fragments arranged by size:
2351 1146 310 146
EcoICRI GAG'CTC
Cuts at: 965 965
Size: 3953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 805 1778 2210 2957 3225 532
Size: 273 973 432 747 268 1260
Fragments arranged by size:
1260 973 747 432 273 268
EcoO109I rG'GnC_Cy
Cuts at: 141 516 517 609 696 1092 141
Size: 375 1 92 87 396 3002
Fragments arranged by size:
3002 396 375 92 87 1
EcoRI G'AATT_C
Cuts at: 957 957
Size: 3953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1972 1972
Size: 3953
FspI TGC'GCA
Cuts at: 880 1734 880
Size: 854 3099
HaeII r_GCGC'y
Cuts at: 1387 1635 2657 3636 1387
Size: 248 1022 979 1704
Fragments arranged by size:
1704 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 403 435 849 881 1795 1825 1827
Size: 32 55 32 414 32 914 30 2
Cuts at: 1827 1857 316
Size: 30 2412
Fragments arranged by size:
2412 914 414 55 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 992 992
Size: 3953
KasI G'GCGC_C
Cuts at: 1383 1631 1383
Size: 248 3705
KpnI G_GTAC'C
Cuts at: 973 973
Size: 3953
MluI A'CGCG_T
Cuts at: 3386 3386
Size: 3953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 963 2624 2808 963
Size: 1661 184 2108
Fragments arranged by size:
2108 1661 184
MscI TGG'CCA
Cuts at: 1714 1714
Size: 3953
MslI CAynn'nnrTG
Cuts at: 634 1942 2069 634
Size: 1308 127 2518
Fragments arranged by size:
2518 1308 127
MspA1I CmG'CkG
Cuts at: 499 876 1064 1378 1738 2751 2996 499
Size: 377 188 314 360 1013 245 1456
Fragments arranged by size:
1456 1013 377 360 314 245 188
NaeI GCC'GGC
Cuts at: 739 2134 739
Size: 1395 2558
NarI GG'CG_CC
Cuts at: 1384 1632 1384
Size: 248 3705
NcoI C'CATG_G
Cuts at: 107 107
Size: 3953
NdeI CA'TA_TG
Cuts at: 645 645
Size: 3953
NgoMIV G'CCGG_C
Cuts at: 737 2132 737
Size: 1395 2558
NspI r_CATG'y
Cuts at: 1006 1506 2413 1006
Size: 500 907 2546
Fragments arranged by size:
2546 907 500
PciI A'CATG_T
Cuts at: 2409 2409
Size: 3953
PmeI GTTT'AAAC
Cuts at: 952 952
Size: 3953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 3921
PpuMI rG'GwC_Cy
Cuts at: 141 696 141
Size: 555 3398
PspOMI G'GGCC_C
Cuts at: 516 923 516
Size: 407 3546
PstI C_TGCA'G
Cuts at: 1000 1685 1000
Size: 685 3268
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3953
PvuII CAG'CTG
Cuts at: 876 1378 1738 876
Size: 502 360 3091
Fragments arranged by size:
3091 502 360
RsrII CG'GwC_CG
Cuts at: 2148 2148
Size: 3953
SacI G_AGCT'C
Cuts at: 967 967
Size: 3953
SalI G'TCGA_C
Cuts at: 990 990
Size: 3953
SanDI GG'GwC_CC
Cuts at: 141 696 141
Size: 555 3398
SapI GCTCTTCn'nnn_
Cuts at: 1976 2186 1976
Size: 210 3743
SbfI CC_TGCA'GG
Cuts at: 1000 1000
Size: 3953
ScaI AGT'ACT
Cuts at: 3470 3470
Size: 3953
SfcI C'TryA_G
Cuts at: 33 368 996 1681 2674 2865 33
Size: 335 628 685 993 191 1121
Fragments arranged by size:
1121 993 685 628 335 191
SfoI GGC'GCC
Cuts at: 1385 1633 1385
Size: 248 3705
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
SmaI CCC'GGG
Cuts at: 975 975
Size: 3953
SmlI C'TyrA_G
Cuts at: 1108 2023 2515 2777 1108
Size: 915 492 262 2284
Fragments arranged by size:
2284 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3953
SphI G_CATG'C
Cuts at: 1006 1506 1006
Size: 500 3453
StyI C'CwwG_G
Cuts at: 107 503 680 1087 1441 107
Size: 396 177 407 354 2619
Fragments arranged by size:
2619 407 396 354 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 705 1536 1669 2128 2337 705
Size: 831 133 459 209 2321
Fragments arranged by size:
2321 831 459 209 133
TatI w'GTAC_w
Cuts at: 3468 3468
Size: 3953
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 3921
Tth111I GACn'n_nGTC
Cuts at: 1750 1750
Size: 3953
XbaI T'CTAG_A
Cuts at: 984 984
Size: 3953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 484 1448 484
Size: 964 2989
XmaI C'CCGG_G
Cuts at: 973 973
Size: 3953
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BfrBI BmgBI BmtI BsrGI
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI