Restriction map for pF1KE0687
 (Circular) (Six-base) MAPSORT of: pF1KE0687.seq  Check: 2691  from: 1  to: 4397



pF1KE0687  4397 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AccI GT'mk_AC

Cuts at:   1435    1435 
   Size:       4397
 
Acc65I G'GTAC_C

Cuts at:   1413    1413 
   Size:       4397
 
AclI AA'CG_TT

Cuts at:   4237    4237 
   Size:       4397
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1080    2222    2654    3401    3669    1080 
   Size:       1142     432     747     268    1808

  Fragments arranged by size: 

                1808    1142     747     432     268
 
AflIII A'CryG_T

Cuts at:   1749    2853    3830    1749 
   Size:       1104     977    2316

  Fragments arranged by size: 

                2316    1104     977
 
AgeI A'CCGG_T

Cuts at:   2785    2785 
   Size:       4397
 
AleI CACnn'nnGTG

Cuts at:    478     478 
   Size:       4397
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    670     670 
   Size:       4397
 
AlwNI CAG_nnn'CTG

Cuts at:   1702    3269    1702 
   Size:       1567    2830
 
ApaLI G'TGCA_C

Cuts at:   3167    3167 
   Size:       4397
 
ApoI r'AATT_y

Cuts at:    829    1401    3750    4006     829 
   Size:        572    2349     256    1220

  Fragments arranged by size: 

                2349    1220     572     256
 
AseI AT'TA_AT

Cuts at:     20     799      20 
   Size:        779    3618
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4397
 
AvaI C'yCGr_G

Cuts at:    166     554     936    1417     166 
   Size:        388     382     481    3146

  Fragments arranged by size: 

                3146     481     388     382
 
BamHI G'GATC_C

Cuts at:   1422    1422 
   Size:       4397
 
BanI G'GyrC_C

Cuts at:    472     605     654    1380    1413    1827    2075    2110     472 
   Size:        133      49     726      33     414     248      35    2759

  Fragments arranged by size: 

                2759     726     414     248     133      49      35      33
            
 
BanII G_rGCy'C

Cuts at:    173     953    1411    2441     173 
   Size:        780     458    1030    2129

  Fragments arranged by size: 

                2129    1030     780     458
 
BbeI G_GCGC'C

Cuts at:   1831    2079    1831 
   Size:        248    4149
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1035    1069    1035 
   Size:         34    4363
 
BciVI GTATCCnnnnn_n'

Cuts at:   2295    3056    4397    2295 
   Size:        761    1341    2295

  Fragments arranged by size: 

                2295    1341     761
 
BclI T'GATC_A

Cuts at:   1917    1917 
   Size:       4397
 
BglI GCCn_nnn'nGGC

Cuts at:   1114    1114 
   Size:       4397
 
BglII A'GATC_T

Cuts at:   1912    3949    1912 
   Size:       2037    2360
 
BlpI GC'TnA_GC

Cuts at:    268    1509     268 
   Size:       1241    3156
 
Bme1580I G_kGCm'C

Cuts at:    475    2022    2115    3171     475 
   Size:       1547      93    1056    1701

  Fragments arranged by size: 

                1701    1547    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1609    1690    2025    4169    1609 
   Size:         81     335    2144    1837

  Fragments arranged by size: 

                2144    1837     335      81
 
BmtI G_CTAG'C

Cuts at:    842     842 
   Size:       4397
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2764    2764 
   Size:       4397
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    967     967 
   Size:       4397
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    403    1573    2452    2944    3242     403 
   Size:       1170     879     492     298    1558

  Fragments arranged by size: 

                1558    1170     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4101    4101 
   Size:       4397
 
BsaAI yAC'GTr

Cuts at:   1750    1750 
   Size:       4397
 
BsaBI GATnn'nnATC

Cuts at:      6    1090    1911    3948       6 
   Size:       1084     821    2037     455

  Fragments arranged by size: 

                2037    1084     821     455
 
BsaHI Gr'CG_yC

Cuts at:   1828    2076    2778    4274    1828 
   Size:        248     702    1496    1951

  Fragments arranged by size: 

                1951    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    995    1586    2107    2785    3059    3206    3617     995 
   Size:        591     521     678     274     147     411    1775

  Fragments arranged by size: 

                1775     678     591     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1114    1144    1177    1207    1114 
   Size:         30      33      30    4304

  Fragments arranged by size: 

                4304      33      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    143     337    1165    1182    1327    1330     143 
   Size:        194     828      17     145       3    3210

  Fragments arranged by size: 

                3210     828     194     145      17       3
 
BseYI C'CCAG_C

Cuts at:    276    1822    2580    3157    3653     276 
   Size:       1546     758     577     496    1020

  Fragments arranged by size: 

                1546    1020     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    256     256 
   Size:       4397
 
BsiEI CG_ry'CG

Cuts at:    104    1939    1985    3193     104 
   Size:       1835      46    1208    1308

  Fragments arranged by size: 

                1835    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    953    1411    2189    2379    3171     953 
   Size:        458     778     190     792    2179

  Fragments arranged by size: 

                2179     792     778     458     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    175     175 
   Size:       4397
 
Bsp1286I G_dGCh'C

Cuts at:    173     475     953    1411    2022    2115    2189    2379    2441 
   Size:        302     478     458     611      93      74     190      62

Cuts at:   2441    3171     173 
   Size:        730    1399

  Fragments arranged by size: 

                1399     730     611     478     458     302     190      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:    995    3617     995 
   Size:       2622    1775
 
BspHI T'CATG_A

Cuts at:    623    1223    3573     623 
   Size:        600    2350    1447

  Fragments arranged by size: 

                2350    1447     600
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    926    1447    1661    1963    2344     926 
   Size:        521     214     302     381    2979

  Fragments arranged by size: 

                2979     521     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2689    2743    4227    4392      50 
   Size:       2639      54    1484     165      55

  Fragments arranged by size: 

                2639    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2309    2309 
   Size:       4397
 
BsrFI r'CCGG_y

Cuts at:    967    2395    2576    2785     967 
   Size:       1428     181     209    2579

  Fragments arranged by size: 

                2579    1428     209     181
 
BssSI C'ACGA_G

Cuts at:   2668    3026    2668 
   Size:        358    4039
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1774    1774 
   Size:       4397
 
BstBI TT'CG_AA

Cuts at:   2758    2758 
   Size:       4397
 
BstEII G'GTnAC_C

Cuts at:    330     330 
   Size:       4397
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114    1023     114 
   Size:        909    3488
 
BstYI r'GATC_y

Cuts at:      1    1422    1889    1912    2247    2493    3505    3591    3603 
   Size:       1421     467      23     335     246    1012      86      12

Cuts at:   3603    3949       1 
   Size:        346     449

  Fragments arranged by size: 

                1421    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4397
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    920    2347    2528    2691     920 
   Size:       1427     181     163    2626

  Fragments arranged by size: 

                2626    1427     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1640    1640 
   Size:       4397
 
DraI TTT'AAA

Cuts at:   1396    1396 
   Size:       4397
 
DrdI GACnn_nn'nnGTC

Cuts at:   2103    2961    2103 
   Size:        858    3539
 
EaeI y'GGCC_r

Cuts at:   1025    1253    1982    2156    2547    2574    3919    1025 
   Size:        228     729     174     391      27    1345    1503

  Fragments arranged by size: 

                1503    1345     729     391     228     174      27
 
EagI C'GGCC_G

Cuts at:   1982    1982 
   Size:       4397
 
EarI CTCTTCn'nnn_

Cuts at:    388    1075    2420    2630     388 
   Size:        687    1345     210    2155

  Fragments arranged by size: 

                2155    1345     687     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1228    2605    2915    3061    4207    1228 
   Size:       1377     310     146    1146    1418

  Fragments arranged by size: 

                1418    1377    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    951    1409     951 
   Size:        458    3939
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    967    1080    2222    2654    3401    3669     967 
   Size:        113    1142     432     747     268    1695

  Fragments arranged by size: 

                1695    1142     747     432     268     113
 
EcoO109I rG'GnC_Cy

Cuts at:    932    1536     932 
   Size:        604    3793
 
EcoRI G'AATT_C

Cuts at:   1401    1401 
   Size:       4397
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2416    2416 
   Size:       4397
 
FspI TGC'GCA

Cuts at:   2178    2178 
   Size:       4397
 
HaeII r_GCGC'y

Cuts at:    443    1103    1143    1831    2079    3101    4080     443 
   Size:        660      40     688     248    1022     979     760

  Fragments arranged by size: 

                1022     979     760     688     660     248      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1114    1146    2239    2269    2271    2301    1114 
   Size:         32    1093      30       2      30    3210

  Fragments arranged by size: 

                3210    1093      32      30      30       2
 
HincII GTy'rAC

Cuts at:    120    1203    1436     120 
   Size:       1083     233    3081

  Fragments arranged by size: 

                3081    1083     233
 
KasI G'GCGC_C

Cuts at:   1827    2075    1827 
   Size:        248    4149
 
KpnI G_GTAC'C

Cuts at:   1417    1417 
   Size:       4397
 
MluI A'CGCG_T

Cuts at:   3830    3830 
   Size:       4397
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3068    3252    3068 
   Size:        184    4213
 
MscI TGG'CCA

Cuts at:   1027    1255    2158    1027 
   Size:        228     903    3266

  Fragments arranged by size: 

                3266     903     228
 
MslI CAynn'nnrTG

Cuts at:    112     298     478    1370    2386    2513     112 
   Size:        186     180     892    1016     127    1996

  Fragments arranged by size: 

                1996    1016     892     186     180     127
 
MspA1I CmG'CkG

Cuts at:    232     887     960    1508    1822    2182    3195    3440     232 
   Size:        655      73     548     314     360    1013     245    1189

  Fragments arranged by size: 

                1189    1013     655     548     360     314     245      73
            
 
NaeI GCC'GGC

Cuts at:   2578    2578 
   Size:       4397
 
NarI GG'CG_CC

Cuts at:   1828    2076    1828 
   Size:        248    4149
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4397
 
NdeI CA'TA_TG

Cuts at:    792    1367     792 
   Size:        575    3822
 
NgoMIV G'CCGG_C

Cuts at:   2576    2576 
   Size:       4397
 
NheI G'CTAG_C

Cuts at:    838     838 
   Size:       4397
 
NspI r_CATG'y

Cuts at:    393    1050    1450    1950    2857     393 
   Size:        657     400     500     907    1933

  Fragments arranged by size: 

                1933     907     657     500     400
 
PasI CC'CwG_GG

Cuts at:   1288    1288 
   Size:       4397
 
PciI A'CATG_T

Cuts at:   2853    2853 
   Size:       4397
 
PflMI CCAn_nnn'nTGG

Cuts at:    221    1109     221 
   Size:        888    3509
 
PfoI T'CCnGG_A

Cuts at:    768     768 
   Size:       4397
 
PmeI GTTT'AAAC

Cuts at:   1396    1396 
   Size:       4397
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    155     187    3536    3568     155 
   Size:         32    3349      32     984

  Fragments arranged by size: 

                3349     984      32      32
 
PstI C_TGCA'G

Cuts at:   1444    2129    1444 
   Size:        685    3712
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4397
 
PvuII CAG'CTG

Cuts at:    960    1822    2182     960 
   Size:        862     360    3175

  Fragments arranged by size: 

                3175     862     360
 
RsrII CG'GwC_CG

Cuts at:   2592    2592 
   Size:       4397
 
SacI G_AGCT'C

Cuts at:    953    1411     953 
   Size:        458    3939
 
SalI G'TCGA_C

Cuts at:   1434    1434 
   Size:       4397
 
SapI GCTCTTCn'nnn_

Cuts at:   2420    2630    2420 
   Size:        210    4187
 
SbfI CC_TGCA'GG

Cuts at:   1444    1444 
   Size:       4397
 
ScaI AGT'ACT

Cuts at:   3914    3914 
   Size:       4397
 
SexAI A'CCwGG_T

Cuts at:    601     601 
   Size:       4397
 
SfcI C'TryA_G

Cuts at:     33    1440    2125    3118    3309      33 
   Size:       1407     685     993     191    1121

  Fragments arranged by size: 

                1407    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   1829    2077    1829 
   Size:        248    4149
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4397
 
SmaI CCC'GGG

Cuts at:    556    1419     556 
   Size:        863    3534
 
SmlI C'TyrA_G

Cuts at:    418    1552    2467    2959    3221     418 
   Size:       1134     915     492     262    1594

  Fragments arranged by size: 

                1594    1134     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4397
 
SphI G_CATG'C

Cuts at:    393    1050    1450    1950     393 
   Size:        657     400     500    2840

  Fragments arranged by size: 

                2840     657     500     400
 
StyI C'CwwG_G

Cuts at:    107    1531    1885     107 
   Size:       1424     354    2619

  Fragments arranged by size: 

                2619    1424     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    899    1980    2113    2572    2781     899 
   Size:       1081     133     459     209    2515

  Fragments arranged by size: 

                2515    1081     459     209     133
 
TatI w'GTAC_w

Cuts at:    248    3912     248 
   Size:       3664     733
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3914    3946    3914 
   Size:         32    4365
 
Tth111I GACn'n_nGTC

Cuts at:   2194    2194 
   Size:       4397
 
XbaI T'CTAG_A

Cuts at:    516    1428     516 
   Size:        912    3485
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    465    1892     465 
   Size:       1427    2970
 
XmaI C'CCGG_G

Cuts at:    554    1417     554 
   Size:        863    3534
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI    BbvCI    BfrBI    BmgBI   Bpu10I    BsiWI
     BsmI    BsrGI   BssHII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NotI
     NruI     NsiI     PacI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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