Restriction map for
pF1KE0687
(Circular) (Six-base) MAPSORT of: pF1KE0687.seq Check: 2691 from: 1 to: 4397
pF1KE0687 4397 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1435 1435
Size: 4397
Acc65I G'GTAC_C
Cuts at: 1413 1413
Size: 4397
AclI AA'CG_TT
Cuts at: 4237 4237
Size: 4397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1080 2222 2654 3401 3669 1080
Size: 1142 432 747 268 1808
Fragments arranged by size:
1808 1142 747 432 268
AflIII A'CryG_T
Cuts at: 1749 2853 3830 1749
Size: 1104 977 2316
Fragments arranged by size:
2316 1104 977
AgeI A'CCGG_T
Cuts at: 2785 2785
Size: 4397
AleI CACnn'nnGTG
Cuts at: 478 478
Size: 4397
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 670 670
Size: 4397
AlwNI CAG_nnn'CTG
Cuts at: 1702 3269 1702
Size: 1567 2830
ApaLI G'TGCA_C
Cuts at: 3167 3167
Size: 4397
ApoI r'AATT_y
Cuts at: 829 1401 3750 4006 829
Size: 572 2349 256 1220
Fragments arranged by size:
2349 1220 572 256
AseI AT'TA_AT
Cuts at: 20 799 20
Size: 779 3618
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
AvaI C'yCGr_G
Cuts at: 166 554 936 1417 166
Size: 388 382 481 3146
Fragments arranged by size:
3146 481 388 382
BamHI G'GATC_C
Cuts at: 1422 1422
Size: 4397
BanI G'GyrC_C
Cuts at: 472 605 654 1380 1413 1827 2075 2110 472
Size: 133 49 726 33 414 248 35 2759
Fragments arranged by size:
2759 726 414 248 133 49 35 33
BanII G_rGCy'C
Cuts at: 173 953 1411 2441 173
Size: 780 458 1030 2129
Fragments arranged by size:
2129 1030 780 458
BbeI G_GCGC'C
Cuts at: 1831 2079 1831
Size: 248 4149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1035 1069 1035
Size: 34 4363
BciVI GTATCCnnnnn_n'
Cuts at: 2295 3056 4397 2295
Size: 761 1341 2295
Fragments arranged by size:
2295 1341 761
BclI T'GATC_A
Cuts at: 1917 1917
Size: 4397
BglI GCCn_nnn'nGGC
Cuts at: 1114 1114
Size: 4397
BglII A'GATC_T
Cuts at: 1912 3949 1912
Size: 2037 2360
BlpI GC'TnA_GC
Cuts at: 268 1509 268
Size: 1241 3156
Bme1580I G_kGCm'C
Cuts at: 475 2022 2115 3171 475
Size: 1547 93 1056 1701
Fragments arranged by size:
1701 1547 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1609 1690 2025 4169 1609
Size: 81 335 2144 1837
Fragments arranged by size:
2144 1837 335 81
BmtI G_CTAG'C
Cuts at: 842 842
Size: 4397
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2764 2764
Size: 4397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 967
Size: 4397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1573 2452 2944 3242 403
Size: 1170 879 492 298 1558
Fragments arranged by size:
1558 1170 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4101 4101
Size: 4397
BsaAI yAC'GTr
Cuts at: 1750 1750
Size: 4397
BsaBI GATnn'nnATC
Cuts at: 6 1090 1911 3948 6
Size: 1084 821 2037 455
Fragments arranged by size:
2037 1084 821 455
BsaHI Gr'CG_yC
Cuts at: 1828 2076 2778 4274 1828
Size: 248 702 1496 1951
Fragments arranged by size:
1951 1496 702 248
BsaWI w'CCGG_w
Cuts at: 995 1586 2107 2785 3059 3206 3617 995
Size: 591 521 678 274 147 411 1775
Fragments arranged by size:
1775 678 591 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1114 1144 1177 1207 1114
Size: 30 33 30 4304
Fragments arranged by size:
4304 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 143 337 1165 1182 1327 1330 143
Size: 194 828 17 145 3 3210
Fragments arranged by size:
3210 828 194 145 17 3
BseYI C'CCAG_C
Cuts at: 276 1822 2580 3157 3653 276
Size: 1546 758 577 496 1020
Fragments arranged by size:
1546 1020 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4397
BsiEI CG_ry'CG
Cuts at: 104 1939 1985 3193 104
Size: 1835 46 1208 1308
Fragments arranged by size:
1835 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 953 1411 2189 2379 3171 953
Size: 458 778 190 792 2179
Fragments arranged by size:
2179 792 778 458 190
BsmBI CGTCTCn'nnnn_
Cuts at: 175 175
Size: 4397
Bsp1286I G_dGCh'C
Cuts at: 173 475 953 1411 2022 2115 2189 2379 2441
Size: 302 478 458 611 93 74 190 62
Cuts at: 2441 3171 173
Size: 730 1399
Fragments arranged by size:
1399 730 611 478 458 302 190 93
74 62
BspEI T'CCGG_A
Cuts at: 995 3617 995
Size: 2622 1775
BspHI T'CATG_A
Cuts at: 623 1223 3573 623
Size: 600 2350 1447
Fragments arranged by size:
2350 1447 600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 926 1447 1661 1963 2344 926
Size: 521 214 302 381 2979
Fragments arranged by size:
2979 521 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2689 2743 4227 4392 50
Size: 2639 54 1484 165 55
Fragments arranged by size:
2639 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2309 2309
Size: 4397
BsrFI r'CCGG_y
Cuts at: 967 2395 2576 2785 967
Size: 1428 181 209 2579
Fragments arranged by size:
2579 1428 209 181
BssSI C'ACGA_G
Cuts at: 2668 3026 2668
Size: 358 4039
BstAPI GCAn_nnn'nTGC
Cuts at: 1774 1774
Size: 4397
BstBI TT'CG_AA
Cuts at: 2758 2758
Size: 4397
BstEII G'GTnAC_C
Cuts at: 330 330
Size: 4397
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1023 114
Size: 909 3488
BstYI r'GATC_y
Cuts at: 1 1422 1889 1912 2247 2493 3505 3591 3603
Size: 1421 467 23 335 246 1012 86 12
Cuts at: 3603 3949 1
Size: 346 449
Fragments arranged by size:
1421 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4397
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 920 2347 2528 2691 920
Size: 1427 181 163 2626
Fragments arranged by size:
2626 1427 181 163
BtsI GCAGTG_nn'
Cuts at: 1640 1640
Size: 4397
DraI TTT'AAA
Cuts at: 1396 1396
Size: 4397
DrdI GACnn_nn'nnGTC
Cuts at: 2103 2961 2103
Size: 858 3539
EaeI y'GGCC_r
Cuts at: 1025 1253 1982 2156 2547 2574 3919 1025
Size: 228 729 174 391 27 1345 1503
Fragments arranged by size:
1503 1345 729 391 228 174 27
EagI C'GGCC_G
Cuts at: 1982 1982
Size: 4397
EarI CTCTTCn'nnn_
Cuts at: 388 1075 2420 2630 388
Size: 687 1345 210 2155
Fragments arranged by size:
2155 1345 687 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1228 2605 2915 3061 4207 1228
Size: 1377 310 146 1146 1418
Fragments arranged by size:
1418 1377 1146 310 146
EcoICRI GAG'CTC
Cuts at: 951 1409 951
Size: 458 3939
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1080 2222 2654 3401 3669 967
Size: 113 1142 432 747 268 1695
Fragments arranged by size:
1695 1142 747 432 268 113
EcoO109I rG'GnC_Cy
Cuts at: 932 1536 932
Size: 604 3793
EcoRI G'AATT_C
Cuts at: 1401 1401
Size: 4397
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4397
FspI TGC'GCA
Cuts at: 2178 2178
Size: 4397
HaeII r_GCGC'y
Cuts at: 443 1103 1143 1831 2079 3101 4080 443
Size: 660 40 688 248 1022 979 760
Fragments arranged by size:
1022 979 760 688 660 248 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1114 1146 2239 2269 2271 2301 1114
Size: 32 1093 30 2 30 3210
Fragments arranged by size:
3210 1093 32 30 30 2
HincII GTy'rAC
Cuts at: 120 1203 1436 120
Size: 1083 233 3081
Fragments arranged by size:
3081 1083 233
KasI G'GCGC_C
Cuts at: 1827 2075 1827
Size: 248 4149
KpnI G_GTAC'C
Cuts at: 1417 1417
Size: 4397
MluI A'CGCG_T
Cuts at: 3830 3830
Size: 4397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3068 3252 3068
Size: 184 4213
MscI TGG'CCA
Cuts at: 1027 1255 2158 1027
Size: 228 903 3266
Fragments arranged by size:
3266 903 228
MslI CAynn'nnrTG
Cuts at: 112 298 478 1370 2386 2513 112
Size: 186 180 892 1016 127 1996
Fragments arranged by size:
1996 1016 892 186 180 127
MspA1I CmG'CkG
Cuts at: 232 887 960 1508 1822 2182 3195 3440 232
Size: 655 73 548 314 360 1013 245 1189
Fragments arranged by size:
1189 1013 655 548 360 314 245 73
NaeI GCC'GGC
Cuts at: 2578 2578
Size: 4397
NarI GG'CG_CC
Cuts at: 1828 2076 1828
Size: 248 4149
NcoI C'CATG_G
Cuts at: 107 107
Size: 4397
NdeI CA'TA_TG
Cuts at: 792 1367 792
Size: 575 3822
NgoMIV G'CCGG_C
Cuts at: 2576 2576
Size: 4397
NheI G'CTAG_C
Cuts at: 838 838
Size: 4397
NspI r_CATG'y
Cuts at: 393 1050 1450 1950 2857 393
Size: 657 400 500 907 1933
Fragments arranged by size:
1933 907 657 500 400
PasI CC'CwG_GG
Cuts at: 1288 1288
Size: 4397
PciI A'CATG_T
Cuts at: 2853 2853
Size: 4397
PflMI CCAn_nnn'nTGG
Cuts at: 221 1109 221
Size: 888 3509
PfoI T'CCnGG_A
Cuts at: 768 768
Size: 4397
PmeI GTTT'AAAC
Cuts at: 1396 1396
Size: 4397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 155 187 3536 3568 155
Size: 32 3349 32 984
Fragments arranged by size:
3349 984 32 32
PstI C_TGCA'G
Cuts at: 1444 2129 1444
Size: 685 3712
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4397
PvuII CAG'CTG
Cuts at: 960 1822 2182 960
Size: 862 360 3175
Fragments arranged by size:
3175 862 360
RsrII CG'GwC_CG
Cuts at: 2592 2592
Size: 4397
SacI G_AGCT'C
Cuts at: 953 1411 953
Size: 458 3939
SalI G'TCGA_C
Cuts at: 1434 1434
Size: 4397
SapI GCTCTTCn'nnn_
Cuts at: 2420 2630 2420
Size: 210 4187
SbfI CC_TGCA'GG
Cuts at: 1444 1444
Size: 4397
ScaI AGT'ACT
Cuts at: 3914 3914
Size: 4397
SexAI A'CCwGG_T
Cuts at: 601 601
Size: 4397
SfcI C'TryA_G
Cuts at: 33 1440 2125 3118 3309 33
Size: 1407 685 993 191 1121
Fragments arranged by size:
1407 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1829 2077 1829
Size: 248 4149
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
SmaI CCC'GGG
Cuts at: 556 1419 556
Size: 863 3534
SmlI C'TyrA_G
Cuts at: 418 1552 2467 2959 3221 418
Size: 1134 915 492 262 1594
Fragments arranged by size:
1594 1134 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4397
SphI G_CATG'C
Cuts at: 393 1050 1450 1950 393
Size: 657 400 500 2840
Fragments arranged by size:
2840 657 500 400
StyI C'CwwG_G
Cuts at: 107 1531 1885 107
Size: 1424 354 2619
Fragments arranged by size:
2619 1424 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 899 1980 2113 2572 2781 899
Size: 1081 133 459 209 2515
Fragments arranged by size:
2515 1081 459 209 133
TatI w'GTAC_w
Cuts at: 248 3912 248
Size: 3664 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3914 3946 3914
Size: 32 4365
Tth111I GACn'n_nGTC
Cuts at: 2194 2194
Size: 4397
XbaI T'CTAG_A
Cuts at: 516 1428 516
Size: 912 3485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 465 1892 465
Size: 1427 2970
XmaI C'CCGG_G
Cuts at: 554 1417 554
Size: 863 3534
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI Bpu10I BsiWI
BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NotI
NruI NsiI PacI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI