(Circular) (Six-base) MAPSORT of: pF1KE0687.seq Check: 2691 from: 1 to: 4397 pF1KE0687 4397 bp With 182 enzymes: SgfI * October 7, 2009 14:27 .. AccI GT'mk_AC Cuts at: 1435 1435 Size: 4397 Acc65I G'GTAC_C Cuts at: 1413 1413 Size: 4397 AclI AA'CG_TT Cuts at: 4237 4237 Size: 4397 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1080 2222 2654 3401 3669 1080 Size: 1142 432 747 268 1808 Fragments arranged by size: 1808 1142 747 432 268 AflIII A'CryG_T Cuts at: 1749 2853 3830 1749 Size: 1104 977 2316 Fragments arranged by size: 2316 1104 977 AgeI A'CCGG_T Cuts at: 2785 2785 Size: 4397 AleI CACnn'nnGTG Cuts at: 478 478 Size: 4397 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 670 670 Size: 4397 AlwNI CAG_nnn'CTG Cuts at: 1702 3269 1702 Size: 1567 2830 ApaLI G'TGCA_C Cuts at: 3167 3167 Size: 4397 ApoI r'AATT_y Cuts at: 829 1401 3750 4006 829 Size: 572 2349 256 1220 Fragments arranged by size: 2349 1220 572 256 AseI AT'TA_AT Cuts at: 20 799 20 Size: 779 3618 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4397 AvaI C'yCGr_G Cuts at: 166 554 936 1417 166 Size: 388 382 481 3146 Fragments arranged by size: 3146 481 388 382 BamHI G'GATC_C Cuts at: 1422 1422 Size: 4397 BanI G'GyrC_C Cuts at: 472 605 654 1380 1413 1827 2075 2110 472 Size: 133 49 726 33 414 248 35 2759 Fragments arranged by size: 2759 726 414 248 133 49 35 33 BanII G_rGCy'C Cuts at: 173 953 1411 2441 173 Size: 780 458 1030 2129 Fragments arranged by size: 2129 1030 780 458 BbeI G_GCGC'C Cuts at: 1831 2079 1831 Size: 248 4149 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1035 1069 1035 Size: 34 4363 BciVI GTATCCnnnnn_n' Cuts at: 2295 3056 4397 2295 Size: 761 1341 2295 Fragments arranged by size: 2295 1341 761 BclI T'GATC_A Cuts at: 1917 1917 Size: 4397 BglI GCCn_nnn'nGGC Cuts at: 1114 1114 Size: 4397 BglII A'GATC_T Cuts at: 1912 3949 1912 Size: 2037 2360 BlpI GC'TnA_GC Cuts at: 268 1509 268 Size: 1241 3156 Bme1580I G_kGCm'C Cuts at: 475 2022 2115 3171 475 Size: 1547 93 1056 1701 Fragments arranged by size: 1701 1547 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1609 1690 2025 4169 1609 Size: 81 335 2144 1837 Fragments arranged by size: 2144 1837 335 81 BmtI G_CTAG'C Cuts at: 842 842 Size: 4397 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2764 2764 Size: 4397 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 967 967 Size: 4397 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 403 1573 2452 2944 3242 403 Size: 1170 879 492 298 1558 Fragments arranged by size: 1558 1170 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4101 4101 Size: 4397 BsaAI yAC'GTr Cuts at: 1750 1750 Size: 4397 BsaBI GATnn'nnATC Cuts at: 6 1090 1911 3948 6 Size: 1084 821 2037 455 Fragments arranged by size: 2037 1084 821 455 BsaHI Gr'CG_yC Cuts at: 1828 2076 2778 4274 1828 Size: 248 702 1496 1951 Fragments arranged by size: 1951 1496 702 248 BsaWI w'CCGG_w Cuts at: 995 1586 2107 2785 3059 3206 3617 995 Size: 591 521 678 274 147 411 1775 Fragments arranged by size: 1775 678 591 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1114 1144 1177 1207 1114 Size: 30 33 30 4304 Fragments arranged by size: 4304 33 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 143 337 1165 1182 1327 1330 143 Size: 194 828 17 145 3 3210 Fragments arranged by size: 3210 828 194 145 17 3 BseYI C'CCAG_C Cuts at: 276 1822 2580 3157 3653 276 Size: 1546 758 577 496 1020 Fragments arranged by size: 1546 1020 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 256 256 Size: 4397 BsiEI CG_ry'CG Cuts at: 104 1939 1985 3193 104 Size: 1835 46 1208 1308 Fragments arranged by size: 1835 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 953 1411 2189 2379 3171 953 Size: 458 778 190 792 2179 Fragments arranged by size: 2179 792 778 458 190 BsmBI CGTCTCn'nnnn_ Cuts at: 175 175 Size: 4397 Bsp1286I G_dGCh'C Cuts at: 173 475 953 1411 2022 2115 2189 2379 2441 Size: 302 478 458 611 93 74 190 62 Cuts at: 2441 3171 173 Size: 730 1399 Fragments arranged by size: 1399 730 611 478 458 302 190 93 74 62 BspEI T'CCGG_A Cuts at: 995 3617 995 Size: 2622 1775 BspHI T'CATG_A Cuts at: 623 1223 3573 623 Size: 600 2350 1447 Fragments arranged by size: 2350 1447 600 BspMI ACCTGCnnnn'nnnn_ Cuts at: 926 1447 1661 1963 2344 926 Size: 521 214 302 381 2979 Fragments arranged by size: 2979 521 381 302 214 BsrBI CCG'CTC Cuts at: 50 2689 2743 4227 4392 50 Size: 2639 54 1484 165 55 Fragments arranged by size: 2639 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2309 2309 Size: 4397 BsrFI r'CCGG_y Cuts at: 967 2395 2576 2785 967 Size: 1428 181 209 2579 Fragments arranged by size: 2579 1428 209 181 BssSI C'ACGA_G Cuts at: 2668 3026 2668 Size: 358 4039 BstAPI GCAn_nnn'nTGC Cuts at: 1774 1774 Size: 4397 BstBI TT'CG_AA Cuts at: 2758 2758 Size: 4397 BstEII G'GTnAC_C Cuts at: 330 330 Size: 4397 BstXI CCAn_nnnn'nTGG Cuts at: 114 1023 114 Size: 909 3488 BstYI r'GATC_y Cuts at: 1 1422 1889 1912 2247 2493 3505 3591 3603 Size: 1421 467 23 335 246 1012 86 12 Cuts at: 3603 3949 1 Size: 346 449 Fragments arranged by size: 1421 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4397 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 920 2347 2528 2691 920 Size: 1427 181 163 2626 Fragments arranged by size: 2626 1427 181 163 BtsI GCAGTG_nn' Cuts at: 1640 1640 Size: 4397 DraI TTT'AAA Cuts at: 1396 1396 Size: 4397 DrdI GACnn_nn'nnGTC Cuts at: 2103 2961 2103 Size: 858 3539 EaeI y'GGCC_r Cuts at: 1025 1253 1982 2156 2547 2574 3919 1025 Size: 228 729 174 391 27 1345 1503 Fragments arranged by size: 1503 1345 729 391 228 174 27 EagI C'GGCC_G Cuts at: 1982 1982 Size: 4397 EarI CTCTTCn'nnn_ Cuts at: 388 1075 2420 2630 388 Size: 687 1345 210 2155 Fragments arranged by size: 2155 1345 687 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1228 2605 2915 3061 4207 1228 Size: 1377 310 146 1146 1418 Fragments arranged by size: 1418 1377 1146 310 146 EcoICRI GAG'CTC Cuts at: 951 1409 951 Size: 458 3939 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 967 1080 2222 2654 3401 3669 967 Size: 113 1142 432 747 268 1695 Fragments arranged by size: 1695 1142 747 432 268 113 EcoO109I rG'GnC_Cy Cuts at: 932 1536 932 Size: 604 3793 EcoRI G'AATT_C Cuts at: 1401 1401 Size: 4397 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2416 2416 Size: 4397 FspI TGC'GCA Cuts at: 2178 2178 Size: 4397 HaeII r_GCGC'y Cuts at: 443 1103 1143 1831 2079 3101 4080 443 Size: 660 40 688 248 1022 979 760 Fragments arranged by size: 1022 979 760 688 660 248 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1114 1146 2239 2269 2271 2301 1114 Size: 32 1093 30 2 30 3210 Fragments arranged by size: 3210 1093 32 30 30 2 HincII GTy'rAC Cuts at: 120 1203 1436 120 Size: 1083 233 3081 Fragments arranged by size: 3081 1083 233 KasI G'GCGC_C Cuts at: 1827 2075 1827 Size: 248 4149 KpnI G_GTAC'C Cuts at: 1417 1417 Size: 4397 MluI A'CGCG_T Cuts at: 3830 3830 Size: 4397 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3068 3252 3068 Size: 184 4213 MscI TGG'CCA Cuts at: 1027 1255 2158 1027 Size: 228 903 3266 Fragments arranged by size: 3266 903 228 MslI CAynn'nnrTG Cuts at: 112 298 478 1370 2386 2513 112 Size: 186 180 892 1016 127 1996 Fragments arranged by size: 1996 1016 892 186 180 127 MspA1I CmG'CkG Cuts at: 232 887 960 1508 1822 2182 3195 3440 232 Size: 655 73 548 314 360 1013 245 1189 Fragments arranged by size: 1189 1013 655 548 360 314 245 73 NaeI GCC'GGC Cuts at: 2578 2578 Size: 4397 NarI GG'CG_CC Cuts at: 1828 2076 1828 Size: 248 4149 NcoI C'CATG_G Cuts at: 107 107 Size: 4397 NdeI CA'TA_TG Cuts at: 792 1367 792 Size: 575 3822 NgoMIV G'CCGG_C Cuts at: 2576 2576 Size: 4397 NheI G'CTAG_C Cuts at: 838 838 Size: 4397 NspI r_CATG'y Cuts at: 393 1050 1450 1950 2857 393 Size: 657 400 500 907 1933 Fragments arranged by size: 1933 907 657 500 400 PasI CC'CwG_GG Cuts at: 1288 1288 Size: 4397 PciI A'CATG_T Cuts at: 2853 2853 Size: 4397 PflMI CCAn_nnn'nTGG Cuts at: 221 1109 221 Size: 888 3509 PfoI T'CCnGG_A Cuts at: 768 768 Size: 4397 PmeI GTTT'AAAC Cuts at: 1396 1396 Size: 4397 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 155 187 3536 3568 155 Size: 32 3349 32 984 Fragments arranged by size: 3349 984 32 32 PstI C_TGCA'G Cuts at: 1444 2129 1444 Size: 685 3712 PvuI CG_AT'CG Cuts at: 104 104 Size: 4397 PvuII CAG'CTG Cuts at: 960 1822 2182 960 Size: 862 360 3175 Fragments arranged by size: 3175 862 360 RsrII CG'GwC_CG Cuts at: 2592 2592 Size: 4397 SacI G_AGCT'C Cuts at: 953 1411 953 Size: 458 3939 SalI G'TCGA_C Cuts at: 1434 1434 Size: 4397 SapI GCTCTTCn'nnn_ Cuts at: 2420 2630 2420 Size: 210 4187 SbfI CC_TGCA'GG Cuts at: 1444 1444 Size: 4397 ScaI AGT'ACT Cuts at: 3914 3914 Size: 4397 SexAI A'CCwGG_T Cuts at: 601 601 Size: 4397 SfcI C'TryA_G Cuts at: 33 1440 2125 3118 3309 33 Size: 1407 685 993 191 1121 Fragments arranged by size: 1407 1121 993 685 191 SfoI GGC'GCC Cuts at: 1829 2077 1829 Size: 248 4149 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4397 SmaI CCC'GGG Cuts at: 556 1419 556 Size: 863 3534 SmlI C'TyrA_G Cuts at: 418 1552 2467 2959 3221 418 Size: 1134 915 492 262 1594 Fragments arranged by size: 1594 1134 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4397 SphI G_CATG'C Cuts at: 393 1050 1450 1950 393 Size: 657 400 500 2840 Fragments arranged by size: 2840 657 500 400 StyI C'CwwG_G Cuts at: 107 1531 1885 107 Size: 1424 354 2619 Fragments arranged by size: 2619 1424 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 899 1980 2113 2572 2781 899 Size: 1081 133 459 209 2515 Fragments arranged by size: 2515 1081 459 209 133 TatI w'GTAC_w Cuts at: 248 3912 248 Size: 3664 733 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3914 3946 3914 Size: 32 4365 Tth111I GACn'n_nGTC Cuts at: 2194 2194 Size: 4397 XbaI T'CTAG_A Cuts at: 516 1428 516 Size: 912 3485 XcmI CCAnnnn_n'nnnnTGG Cuts at: 465 1892 465 Size: 1427 2970 XmaI C'CCGG_G Cuts at: 554 1417 554 Size: 863 3534 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AloI ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI Bpu10I BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NotI NruI NsiI PacI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI