Restriction map for
pF1KE0690
(Circular) (Six-base) MAPSORT of: pF1KE0690.seq Check: 3914 from: 1 to: 4316
pF1KE0690 4316 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1354 1354
Size: 4316
Acc65I G'GTAC_C
Cuts at: 1332 1332
Size: 4316
AclI AA'CG_TT
Cuts at: 4156 4156
Size: 4316
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1001 2141 2573 3320 3588 811
Size: 190 1140 432 747 268 1539
Fragments arranged by size:
1539 1140 747 432 268 190
AflIII A'CryG_T
Cuts at: 1300 1668 2772 3749 1300
Size: 368 1104 977 1867
Fragments arranged by size:
1867 1104 977 368
AgeI A'CCGG_T
Cuts at: 2704 2704
Size: 4316
AlwNI CAG_nnn'CTG
Cuts at: 1621 3188 1621
Size: 1567 2749
ApaI G_GGCC'C
Cuts at: 387 459 387
Size: 72 4244
ApaLI G'TGCA_C
Cuts at: 778 3086 778
Size: 2308 2008
ApoI r'AATT_y
Cuts at: 448 1320 3669 3925 448
Size: 872 2349 256 839
Fragments arranged by size:
2349 872 839 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4316
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
AvaI C'yCGr_G
Cuts at: 218 249 386 421 887 1336 218
Size: 31 137 35 466 449 3198
Fragments arranged by size:
3198 466 449 137 35 31
BamHI G'GATC_C
Cuts at: 228 608 1341 228
Size: 380 733 3203
Fragments arranged by size:
3203 733 380
BanI G'GyrC_C
Cuts at: 127 183 476 826 882 1332 1746 1994 2029
Size: 56 293 350 56 450 414 248 35
Cuts at: 2029 127
Size: 2414
Fragments arranged by size:
2414 450 414 350 293 248 56 56
35
BanII G_rGCy'C
Cuts at: 387 459 562 632 1094 1330 2360 387
Size: 72 103 70 462 236 1030 2343
Fragments arranged by size:
2343 1030 462 236 103 72 70
BbeI G_GCGC'C
Cuts at: 131 187 480 1750 1998 131
Size: 56 293 1270 248 2449
Fragments arranged by size:
2449 1270 293 248 56
BbvCI CC'TCA_GC
Cuts at: 199 623 948 199
Size: 424 325 3567
Fragments arranged by size:
3567 424 325
BciVI GTATCCnnnnn_n'
Cuts at: 2214 2975 4316 2214
Size: 761 1341 2214
Fragments arranged by size:
2214 1341 761
BclI T'GATC_A
Cuts at: 1836 1836
Size: 4316
BglI GCCn_nnn'nGGC
Cuts at: 265 391 265
Size: 126 4190
BglII A'GATC_T
Cuts at: 1831 3868 1831
Size: 2037 2279
BlpI GC'TnA_GC
Cuts at: 1428 1428
Size: 4316
Bme1580I G_kGCm'C
Cuts at: 387 459 782 1941 2034 3090 387
Size: 72 323 1159 93 1056 1613
Fragments arranged by size:
1613 1159 1056 323 93 72
BmrI ACTGGGnnnn_n'
Cuts at: 166 1528 1609 1944 4088 166
Size: 1362 81 335 2144 394
Fragments arranged by size:
2144 1362 394 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4316
Bpu10I CC'TnA_GC
Cuts at: 199 623 948 199
Size: 424 325 3567
Fragments arranged by size:
3567 424 325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 1492 2371 2863 3161 454
Size: 1038 879 492 298 1609
Fragments arranged by size:
1609 1038 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 580 665 1072 4020 580
Size: 85 407 2948 876
Fragments arranged by size:
2948 876 407 85
BsaAI yAC'GTr
Cuts at: 1669 1669
Size: 4316
BsaBI GATnn'nnATC
Cuts at: 6 1830 3867 6
Size: 1824 2037 455
Fragments arranged by size:
2037 1824 455
BsaHI Gr'CG_yC
Cuts at: 128 184 416 477 1747 1995 2697 4193 128
Size: 56 232 61 1270 248 702 1496 251
Fragments arranged by size:
1496 1270 702 251 248 232 61 56
BsaWI w'CCGG_w
Cuts at: 231 1505 2026 2704 2978 3125 3536 231
Size: 1274 521 678 274 147 411 1011
Fragments arranged by size:
1274 1011 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 517 640 517
Size: 123 4193
BseYI C'CCAG_C
Cuts at: 187 533 708 874 1741 2499 3076 3572 187
Size: 346 175 166 867 758 577 496 931
Fragments arranged by size:
931 867 758 577 496 346 175 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 745 514
Size: 231 4085
BsiEI CG_ry'CG
Cuts at: 104 242 307 1858 1904 3112 104
Size: 138 65 1551 46 1208 1308
Fragments arranged by size:
1551 1308 1208 138 65 46
BsiHKAI G_wGCw'C
Cuts at: 562 782 1094 1330 2108 2298 3090 562
Size: 220 312 236 778 190 792 1788
Fragments arranged by size:
1788 792 778 312 236 220 190
Bsp1286I G_dGCh'C
Cuts at: 387 459 562 632 782 1094 1330 1941 2034
Size: 72 103 70 150 312 236 611 93
Cuts at: 2034 2108 2298 2360 3090 387
Size: 74 190 62 730 1613
Fragments arranged by size:
1613 730 611 312 236 190 150 103
93 74 72 70 62
BspEI T'CCGG_A
Cuts at: 3536 3536
Size: 4316
BspHI T'CATG_A
Cuts at: 3492 3492
Size: 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 581 1366 1580 1882 2263 581
Size: 785 214 302 381 2634
Fragments arranged by size:
2634 785 381 302 214
BsrBI CCG'CTC
Cuts at: 50 156 819 2608 2662 4146 4311 50
Size: 106 663 1789 54 1484 165 55
Fragments arranged by size:
1789 1484 663 165 106 55 54
BsrDI GCAATG_nn'
Cuts at: 528 749 1052 2228 528
Size: 221 303 1176 2616
Fragments arranged by size:
2616 1176 303 221
BsrFI r'CCGG_y
Cuts at: 204 1256 2314 2495 2704 204
Size: 1052 1058 181 209 1816
Fragments arranged by size:
1816 1058 1052 209 181
BssSI C'ACGA_G
Cuts at: 2587 2945 2587
Size: 358 3958
BstAPI GCAn_nnn'nTGC
Cuts at: 1693 1693
Size: 4316
BstBI TT'CG_AA
Cuts at: 2677 2677
Size: 4316
BstYI r'GATC_y
Cuts at: 1 228 404 608 1341 1808 1831 2166 2412
Size: 227 176 204 733 467 23 335 246
Cuts at: 2412 3424 3510 3522 3868 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 733 467 449 346 335 246 227
204 176 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 281 281
Size: 4316
BtgI C'CryG_G
Cuts at: 107 178 224 107
Size: 71 46 4199
Fragments arranged by size:
4199 71 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2266 2447 2610 2266
Size: 181 163 3972
Fragments arranged by size:
3972 181 163
BtsI GCAGTG_nn'
Cuts at: 371 1559 371
Size: 1188 3128
DraI TTT'AAA
Cuts at: 1315 1315
Size: 4316
DrdI GACnn_nn'nnGTC
Cuts at: 1200 2022 2880 1200
Size: 822 858 2636
Fragments arranged by size:
2636 858 822
EaeI y'GGCC_r
Cuts at: 239 304 368 845 1901 2075 2466 2493 3838
Size: 65 64 477 1056 174 391 27 1345
Cuts at: 3838 239
Size: 717
Fragments arranged by size:
1345 1056 717 477 391 174 65 64
27
EagI C'GGCC_G
Cuts at: 239 304 1901 239
Size: 65 1597 2654
Fragments arranged by size:
2654 1597 65
EarI CTCTTCn'nnn_
Cuts at: 2339 2549 2339
Size: 210 4106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 340 1027 2524 2834 2980 4126 340
Size: 687 1497 310 146 1146 530
Fragments arranged by size:
1497 1146 687 530 310 146
EcoICRI GAG'CTC
Cuts at: 560 1092 1328 560
Size: 532 236 3548
Fragments arranged by size:
3548 532 236
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1001 2141 2573 3320 3588 811
Size: 190 1140 432 747 268 1539
Fragments arranged by size:
1539 1140 747 432 268 190
EcoO109I rG'GnC_Cy
Cuts at: 246 383 456 786 1100 1455 246
Size: 137 73 330 314 355 3107
Fragments arranged by size:
3107 355 330 314 137 73
EcoRI G'AATT_C
Cuts at: 448 1320 448
Size: 872 3444
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1079 1239 2335 1079
Size: 160 1096 3060
Fragments arranged by size:
3060 1096 160
FspI TGC'GCA
Cuts at: 2097 2097
Size: 4316
HaeII r_GCGC'y
Cuts at: 131 187 442 480 749 1147 1750 1998 3020
Size: 56 255 38 269 398 603 248 1022
Cuts at: 3020 3999 131
Size: 979 448
Fragments arranged by size:
1022 979 603 448 398 269 255 248
56 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 593 625 2158 2188 2190 2220 593
Size: 32 1533 30 2 30 2689
Fragments arranged by size:
2689 1533 32 30 30 2
HincII GTy'rAC
Cuts at: 1355 1355
Size: 4316
KasI G'GCGC_C
Cuts at: 127 183 476 1746 1994 127
Size: 56 293 1270 248 2449
Fragments arranged by size:
2449 1270 293 248 56
KpnI G_GTAC'C
Cuts at: 1336 1336
Size: 4316
MluI A'CGCG_T
Cuts at: 3749 3749
Size: 4316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2987 3171 2987
Size: 184 4132
MscI TGG'CCA
Cuts at: 2077 2077
Size: 4316
MslI CAynn'nnrTG
Cuts at: 2305 2432 2305
Size: 127 4189
MspA1I CmG'CkG
Cuts at: 180 380 410 738 1427 1741 2101 3114 3359
Size: 200 30 328 689 314 360 1013 245
Cuts at: 3359 180
Size: 1137
Fragments arranged by size:
1137 1013 689 360 328 314 245 200
30
NaeI GCC'GGC
Cuts at: 206 2497 206
Size: 2291 2025
NarI GG'CG_CC
Cuts at: 128 184 477 1747 1995 128
Size: 56 293 1270 248 2449
Fragments arranged by size:
2449 1270 293 248 56
NcoI C'CATG_G
Cuts at: 107 224 107
Size: 117 4199
NgoMIV G'CCGG_C
Cuts at: 204 2495 204
Size: 2291 2025
NotI GC'GGCC_GC
Cuts at: 304 304
Size: 4316
NspI r_CATG'y
Cuts at: 1304 1369 1869 2776 1304
Size: 65 500 907 2844
Fragments arranged by size:
2844 907 500 65
PasI CC'CwG_GG
Cuts at: 1026 1026
Size: 4316
PciI A'CATG_T
Cuts at: 1300 2772 1300
Size: 1472 2844
PfoI T'CCnGG_A
Cuts at: 694 694
Size: 4316
PmeI GTTT'AAAC
Cuts at: 1315 1315
Size: 4316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 3455
Size: 32 4284
PpuMI rG'GwC_Cy
Cuts at: 786 1100 786
Size: 314 4002
PspOMI G'GGCC_C
Cuts at: 383 455 383
Size: 72 4244
PstI C_TGCA'G
Cuts at: 518 1363 2048 518
Size: 845 685 2786
Fragments arranged by size:
2786 845 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4316
PvuII CAG'CTG
Cuts at: 738 1741 2101 738
Size: 1003 360 2953
Fragments arranged by size:
2953 1003 360
RsrII CG'GwC_CG
Cuts at: 2511 2511
Size: 4316
SacI G_AGCT'C
Cuts at: 562 1094 1330 562
Size: 532 236 3548
Fragments arranged by size:
3548 532 236
SacII CC_GC'GG
Cuts at: 181 181
Size: 4316
SalI G'TCGA_C
Cuts at: 1353 1353
Size: 4316
SapI GCTCTTCn'nnn_
Cuts at: 2339 2549 2339
Size: 210 4106
SbfI CC_TGCA'GG
Cuts at: 1363 1363
Size: 4316
ScaI AGT'ACT
Cuts at: 937 3833 937
Size: 2896 1420
SexAI A'CCwGG_T
Cuts at: 1292 1292
Size: 4316
SfcI C'TryA_G
Cuts at: 33 514 733 1359 2044 3037 3228 33
Size: 481 219 626 685 993 191 1121
Fragments arranged by size:
1121 993 685 626 481 219 191
SfoI GGC'GCC
Cuts at: 129 185 478 1748 1996 129
Size: 56 293 1270 248 2449
Fragments arranged by size:
2449 1270 293 248 56
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
SmaI CCC'GGG
Cuts at: 220 251 388 1338 220
Size: 31 137 950 3198
Fragments arranged by size:
3198 950 137 31
SmlI C'TyrA_G
Cuts at: 469 1471 2386 2878 3140 469
Size: 1002 915 492 262 1645
Fragments arranged by size:
1645 1002 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4316
SphI G_CATG'C
Cuts at: 1369 1869 1369
Size: 500 3816
SrfI GCCC'GGGC
Cuts at: 220 220
Size: 4316
StuI AGG'CCT
Cuts at: 259 259
Size: 4316
StyI C'CwwG_G
Cuts at: 107 224 1007 1450 1804 107
Size: 117 783 443 354 2619
Fragments arranged by size:
2619 783 443 354 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1899 2032 2491 2700 1899
Size: 133 459 209 3515
Fragments arranged by size:
3515 459 209 133
TatI w'GTAC_w
Cuts at: 935 3831 935
Size: 2896 1420
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3833 3865 3833
Size: 32 4284
Tth111I GACn'n_nGTC
Cuts at: 1294 2113 1294
Size: 819 3497
XbaI T'CTAG_A
Cuts at: 1347 1347
Size: 4316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 231 840 1811 231
Size: 609 971 2736
Fragments arranged by size:
2736 971 609
XmaI C'CCGG_G
Cuts at: 218 249 386 1336 218
Size: 31 137 950 3198
Fragments arranged by size:
3198 950 137 31
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PasI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BpmI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI
PacI PflMI PmlI PshAI PsiI PspXI PsrI SanDI
SfiI SgrAI SnaBI SspI SwaI XhoI XmnI ZraI