Restriction map for
pF1KE0695
(Circular) (Six-base) MAPSORT of: pF1KE0695.seq Check: 9401 from: 1 to: 3965
pF1KE0695 3965 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1003 1003
Size: 3965
Acc65I G'GTAC_C
Cuts at: 120 981 120
Size: 861 3104
AclI AA'CG_TT
Cuts at: 3805 3805
Size: 3965
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 598 1790 2222 2969 3237 493
Size: 105 1192 432 747 268 1221
Fragments arranged by size:
1221 1192 747 432 268 105
AflIII A'CryG_T
Cuts at: 1317 2421 3398 1317
Size: 1104 977 1884
Fragments arranged by size:
1884 1104 977
AgeI A'CCGG_T
Cuts at: 2353 2353
Size: 3965
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 922 954 922
Size: 32 3933
AlwNI CAG_nnn'CTG
Cuts at: 756 1270 2837 756
Size: 514 1567 1884
Fragments arranged by size:
1884 1567 514
ApaLI G'TGCA_C
Cuts at: 2735 2735
Size: 3965
ApoI r'AATT_y
Cuts at: 969 3318 3574 969
Size: 2349 256 1360
Fragments arranged by size:
2349 1360 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3965
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
AvaI C'yCGr_G
Cuts at: 985 985
Size: 3965
BamHI G'GATC_C
Cuts at: 990 990
Size: 3965
BanI G'GyrC_C
Cuts at: 120 394 555 981 1395 1643 1678 120
Size: 274 161 426 414 248 35 2407
Fragments arranged by size:
2407 426 414 274 248 161 35
BanII G_rGCy'C
Cuts at: 178 336 979 2009 178
Size: 158 643 1030 2134
Fragments arranged by size:
2134 1030 643 158
BbeI G_GCGC'C
Cuts at: 1399 1647 1399
Size: 248 3717
BbsI GAAGACnn'nnnn_
Cuts at: 249 709 818 249
Size: 460 109 3396
Fragments arranged by size:
3396 460 109
BciVI GTATCCnnnnn_n'
Cuts at: 1863 2624 3965 1863
Size: 761 1341 1863
Fragments arranged by size:
1863 1341 761
BclI T'GATC_A
Cuts at: 1485 1485
Size: 3965
BfrBI ATG'CAT
Cuts at: 745 745
Size: 3965
BglI GCCn_nnn'nGGC
Cuts at: 340 340
Size: 3965
BglII A'GATC_T
Cuts at: 1480 3517 1480
Size: 2037 1928
BlpI GC'TnA_GC
Cuts at: 172 1077 172
Size: 905 3060
Bme1580I G_kGCm'C
Cuts at: 1590 1683 2739 1590
Size: 93 1056 2816
Fragments arranged by size:
2816 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 800 1177 1258 1593 3737 800
Size: 377 81 335 2144 1028
Fragments arranged by size:
2144 1028 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 192 2332 192
Size: 2140 1825
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 359
Size: 3965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 2020 2512 2810 1141
Size: 879 492 298 2296
Fragments arranged by size:
2296 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3669 3669
Size: 3965
BsaAI yAC'GTr
Cuts at: 1318 1318
Size: 3965
BsaBI GATnn'nnATC
Cuts at: 6 1479 3516 6
Size: 1473 2037 455
Fragments arranged by size:
2037 1473 455
BsaHI Gr'CG_yC
Cuts at: 1396 1644 2346 3842 1396
Size: 248 702 1496 1519
Fragments arranged by size:
1519 1496 702 248
BsaWI w'CCGG_w
Cuts at: 178 350 1154 1675 2353 2627 2774 3185 178
Size: 172 804 521 678 274 147 411 958
Fragments arranged by size:
958 804 678 521 411 274 172 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 889 919 922 952 889
Size: 30 3 30 3902
Fragments arranged by size:
3902 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 664 910 943 664
Size: 246 33 3686
Fragments arranged by size:
3686 246 33
BseYI C'CCAG_C
Cuts at: 1390 2148 2725 3221 1390
Size: 758 577 496 2134
Fragments arranged by size:
2134 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 191 351 1507 1553 2761 104
Size: 87 160 1156 46 1208 1308
Fragments arranged by size:
1308 1208 1156 160 87 46
BsiHKAI G_wGCw'C
Cuts at: 178 979 1757 1947 2739 178
Size: 801 778 190 792 1404
Fragments arranged by size:
1404 801 792 778 190
BsmI GAATG_Cn'
Cuts at: 745 745
Size: 3965
Bsp1286I G_dGCh'C
Cuts at: 178 336 979 1590 1683 1757 1947 2009 2739
Size: 158 643 611 93 74 190 62 730
Cuts at: 2739 178
Size: 1404
Fragments arranged by size:
1404 730 643 611 190 158 93 74
62
BspEI T'CCGG_A
Cuts at: 178 3185 178
Size: 3007 958
BspHI T'CATG_A
Cuts at: 3141 3141
Size: 3965
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1015 1229 1531 1912 1015
Size: 214 302 381 3068
Fragments arranged by size:
3068 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2257 2311 3795 3960 50
Size: 2207 54 1484 165 55
Fragments arranged by size:
2207 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1877 1877
Size: 3965
BsrFI r'CCGG_y
Cuts at: 1963 2144 2353 1963
Size: 181 209 3575
Fragments arranged by size:
3575 209 181
BssSI C'ACGA_G
Cuts at: 2236 2594 2236
Size: 358 3607
BstAPI GCAn_nnn'nTGC
Cuts at: 322 1342 322
Size: 1020 2945
BstBI TT'CG_AA
Cuts at: 2326 2326
Size: 3965
BstYI r'GATC_y
Cuts at: 1 990 1457 1480 1815 2061 3073 3159 3171
Size: 989 467 23 335 246 1012 86 12
Cuts at: 3171 3517 1
Size: 346 449
Fragments arranged by size:
1012 989 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 185 107
Size: 78 3887
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1915 2096 2259 1915
Size: 181 163 3621
Fragments arranged by size:
3621 181 163
BtsI GCAGTG_nn'
Cuts at: 278 1208 278
Size: 930 3035
DraI TTT'AAA
Cuts at: 964 964
Size: 3965
DrdI GACnn_nn'nnGTC
Cuts at: 1671 2529 1671
Size: 858 3107
EaeI y'GGCC_r
Cuts at: 188 888 1550 1724 2115 2142 3487 188
Size: 700 662 174 391 27 1345 666
Fragments arranged by size:
1345 700 666 662 391 174 27
EagI C'GGCC_G
Cuts at: 188 1550 188
Size: 1362 2603
EarI CTCTTCn'nnn_
Cuts at: 929 1988 2198 929
Size: 1059 210 2696
Fragments arranged by size:
2696 1059 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2173 2483 2629 3775 127
Size: 2046 310 146 1146 317
Fragments arranged by size:
2046 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 176 977 176
Size: 801 3164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 493 598 1790 2222 2969 3237 359
Size: 134 105 1192 432 747 268 1087
Fragments arranged by size:
1192 1087 747 432 268 134 105
EcoO109I rG'GnC_Cy
Cuts at: 1104 1104
Size: 3965
EcoRI G'AATT_C
Cuts at: 969 969
Size: 3965
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1984 1984
Size: 3965
FspI TGC'GCA
Cuts at: 1746 1746
Size: 3965
HaeII r_GCGC'y
Cuts at: 1399 1647 2669 3648 1399
Size: 248 1022 979 1716
Fragments arranged by size:
1716 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1807 1837 1839 1869 1807
Size: 30 2 30 3903
Fragments arranged by size:
3903 30 30 2
HincII GTy'rAC
Cuts at: 1004 1004
Size: 3965
HindIII A'AGCT_T
Cuts at: 505 715 505
Size: 210 3755
KasI G'GCGC_C
Cuts at: 1395 1643 1395
Size: 248 3717
KpnI G_GTAC'C
Cuts at: 124 985 124
Size: 861 3104
MluI A'CGCG_T
Cuts at: 3398 3398
Size: 3965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2636 2820 2636
Size: 184 3781
MscI TGG'CCA
Cuts at: 890 1726 890
Size: 836 3129
MslI CAynn'nnrTG
Cuts at: 1954 2081 1954
Size: 127 3838
MspA1I CmG'CkG
Cuts at: 1076 1390 1750 2763 3008 1076
Size: 314 360 1013 245 2033
Fragments arranged by size:
2033 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2146 2146
Size: 3965
NarI GG'CG_CC
Cuts at: 1396 1644 1396
Size: 248 3717
NcoI C'CATG_G
Cuts at: 107 107
Size: 3965
NgoMIV G'CCGG_C
Cuts at: 2144 2144
Size: 3965
NsiI A_TGCA'T
Cuts at: 747 747
Size: 3965
NspI r_CATG'y
Cuts at: 347 1018 1518 2425 347
Size: 671 500 907 1887
Fragments arranged by size:
1887 907 671 500
PciI A'CATG_T
Cuts at: 2421 2421
Size: 3965
PflMI CCAn_nnn'nTGG
Cuts at: 456 456
Size: 3965
PmeI GTTT'AAAC
Cuts at: 964 964
Size: 3965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 922 954 3104 3136 922
Size: 32 2150 32 1751
Fragments arranged by size:
2150 1751 32 32
PstI C_TGCA'G
Cuts at: 273 1012 1697 273
Size: 739 685 2541
Fragments arranged by size:
2541 739 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3965
PvuII CAG'CTG
Cuts at: 1390 1750 1390
Size: 360 3605
RsrII CG'GwC_CG
Cuts at: 2160 2160
Size: 3965
SacI G_AGCT'C
Cuts at: 178 979 178
Size: 801 3164
SalI G'TCGA_C
Cuts at: 1002 1002
Size: 3965
SapI GCTCTTCn'nnn_
Cuts at: 1988 2198 1988
Size: 210 3755
SbfI CC_TGCA'GG
Cuts at: 1012 1012
Size: 3965
ScaI AGT'ACT
Cuts at: 3482 3482
Size: 3965
SfcI C'TryA_G
Cuts at: 33 269 1008 1693 2686 2877 33
Size: 236 739 685 993 191 1121
Fragments arranged by size:
1121 993 739 685 236 191
SfoI GGC'GCC
Cuts at: 1397 1645 1397
Size: 248 3717
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3965
SmaI CCC'GGG
Cuts at: 987 987
Size: 3965
SmlI C'TyrA_G
Cuts at: 1120 2035 2527 2789 1120
Size: 915 492 262 2296
Fragments arranged by size:
2296 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3965
SphI G_CATG'C
Cuts at: 347 1018 1518 347
Size: 671 500 2794
Fragments arranged by size:
2794 671 500
StyI C'CwwG_G
Cuts at: 107 1099 1453 107
Size: 992 354 2619
Fragments arranged by size:
2619 992 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 473 1548 1681 2140 2349 473
Size: 1075 133 459 209 2089
Fragments arranged by size:
2089 1075 459 209 133
TatI w'GTAC_w
Cuts at: 797 3480 797
Size: 2683 1282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 3933
Tth111I GACn'n_nGTC
Cuts at: 1762 1762
Size: 3965
XbaI T'CTAG_A
Cuts at: 771 996 771
Size: 225 3740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1460 114
Size: 1346 2619
XmaI C'CCGG_G
Cuts at: 985 985
Size: 3965
XmnI GAAnn'nnTTC
Cuts at: 583 583
Size: 3965
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI