Restriction map for
pF1KE0698
(Circular) (Six-base) MAPSORT of: pF1KE0698.seq Check: 3137 from: 1 to: 5402
pF1KE0698 5402 bp
With 182 enzymes: SgfI *
February 2, 2010 10:44 ..
AccI GT'mk_AC
Cuts at: 2440 2440
Size: 5402
Acc65I G'GTAC_C
Cuts at: 2418 2418
Size: 5402
AclI AA'CG_TT
Cuts at: 5242 5242
Size: 5402
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 2245 2302 3227 3659 4012 4674 706
Size: 1539 57 925 432 353 662 1434
Fragments arranged by size:
1539 1434 925 662 432 353 57
AflIII A'CryG_T
Cuts at: 1290 2754 4554 4835 1290
Size: 1464 1800 281 1857
Fragments arranged by size:
1857 1800 1464 281
AleI CACnn'nnGTG
Cuts at: 670 1216 670
Size: 546 4856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1843 1843
Size: 5402
AlwNI CAG_nnn'CTG
Cuts at: 1068 1086 2139 2707 4145 1068
Size: 18 1053 568 1438 2325
Fragments arranged by size:
2325 1438 1053 568 18
ApaLI G'TGCA_C
Cuts at: 4240 4240
Size: 5402
ApoI r'AATT_y
Cuts at: 397 608 811 1398 2057 2406 4755 5011 397
Size: 211 203 587 659 349 2349 256 788
Fragments arranged by size:
2349 788 659 587 349 256 211 203
AseI AT'TA_AT
Cuts at: 20 1589 2378 20
Size: 1569 789 3044
Fragments arranged by size:
3044 1569 789
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5402
AvaI C'yCGr_G
Cuts at: 285 2422 285
Size: 2137 3265
BamHI G'GATC_C
Cuts at: 2427 2427
Size: 5402
BanI G'GyrC_C
Cuts at: 895 1929 2418 2832 3080 3115 895
Size: 1034 489 414 248 35 3182
Fragments arranged by size:
3182 1034 489 414 248 35
BanII G_rGCy'C
Cuts at: 223 1028 2416 3446 223
Size: 805 1388 1030 2179
Fragments arranged by size:
2179 1388 1030 805
BbeI G_GCGC'C
Cuts at: 899 2836 3084 899
Size: 1937 248 3217
Fragments arranged by size:
3217 1937 248
BbsI GAAGACnn'nnnn_
Cuts at: 644 847 1214 1771 2044 644
Size: 203 367 557 273 4002
Fragments arranged by size:
4002 557 367 273 203
BbvCI CC'TCA_GC
Cuts at: 1784 1784
Size: 5402
BciVI GTATCCnnnnn_n'
Cuts at: 3300 4356 5402 3300
Size: 1056 1046 3300
Fragments arranged by size:
3300 1056 1046
BclI T'GATC_A
Cuts at: 2922 2922
Size: 5402
BglII A'GATC_T
Cuts at: 1171 2917 4954 1171
Size: 1746 2037 1619
Fragments arranged by size:
2037 1746 1619
BlpI GC'TnA_GC
Cuts at: 1079 2514 1079
Size: 1435 3967
Bme1580I G_kGCm'C
Cuts at: 736 3027 3120 4244 736
Size: 2291 93 1124 1894
Fragments arranged by size:
2291 1894 1124 93
BmgBI CAC'GTC
Cuts at: 2103 2103
Size: 5402
BmrI ACTGGGnnnn_n'
Cuts at: 1359 2614 2695 3030 5174 1359
Size: 1255 81 335 2144 1587
Fragments arranged by size:
2144 1587 1255 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 698 1770 3769 698
Size: 1072 1999 2331
Fragments arranged by size:
2331 1999 1072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 1709 1991 2068 226
Size: 1483 282 77 3560
Fragments arranged by size:
3560 1483 282 77
Bpu10I CC'TnA_GC
Cuts at: 1784 1784
Size: 5402
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1628 2191 2578 3457 4171 4469 1628
Size: 563 387 879 714 298 2561
Fragments arranged by size:
2561 879 714 563 387 298
BsaI GGTCTCn'nnnn_
Cuts at: 1421 5106 1421
Size: 3685 1717
BsaAI yAC'GTr
Cuts at: 540 1291 2755 540
Size: 751 1464 3187
Fragments arranged by size:
3187 1464 751
BsaBI GATnn'nnATC
Cuts at: 6 587 707 2916 4953 6
Size: 581 120 2209 2037 455
Fragments arranged by size:
2209 2037 581 455 120
BsaHI Gr'CG_yC
Cuts at: 896 2833 3081 3783 5279 896
Size: 1937 248 702 1496 1019
Fragments arranged by size:
1937 1496 1019 702 248
BsaWI w'CCGG_w
Cuts at: 995 1413 2236 2591 3112 3790 4201 4348 4622
Size: 418 823 355 521 678 411 147 274
Cuts at: 4622 995
Size: 1775
Fragments arranged by size:
1775 823 678 521 418 411 355 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 237 267 237
Size: 30 5372
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 2287 400
Size: 1887 3515
BseYI C'CCAG_C
Cuts at: 887 1121 1510 2147 2827 3585 4250 4658 887
Size: 234 389 637 680 758 665 408 1631
Fragments arranged by size:
1631 758 680 665 637 408 389 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1000
Size: 5402
BsiEI CG_ry'CG
Cuts at: 104 2944 2990 4220 104
Size: 2840 46 1230 1286
Fragments arranged by size:
2840 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2416 3194 3384 4244 2416
Size: 778 190 860 3574
Fragments arranged by size:
3574 860 778 190
BsmI GAATG_Cn'
Cuts at: 684 1470 684
Size: 786 4616
Bsp1286I G_dGCh'C
Cuts at: 223 736 1028 2416 3027 3120 3194 3384 3446
Size: 513 292 1388 611 93 74 190 62
Cuts at: 3446 4244 223
Size: 798 1381
Fragments arranged by size:
1388 1381 798 611 513 292 190 93
74 62
BspEI T'CCGG_A
Cuts at: 995 995
Size: 5402
BspHI T'CATG_A
Cuts at: 3834 3834
Size: 5402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1022 2452 2666 2968 3349 1022
Size: 1430 214 302 381 3075
Fragments arranged by size:
3075 1430 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3694 3748 5232 5397 50
Size: 3644 54 1484 165 55
Fragments arranged by size:
3644 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 448 3314 448
Size: 2866 2536
BsrFI r'CCGG_y
Cuts at: 3400 3581 3400
Size: 181 5221
BssSI C'ACGA_G
Cuts at: 3673 4381 3673
Size: 708 4694
BstAPI GCAn_nnn'nTGC
Cuts at: 270 1654 1663 2779 270
Size: 1384 9 1116 2893
Fragments arranged by size:
2893 1384 1116 9
BstBI TT'CG_AA
Cuts at: 3763 3763
Size: 5402
BstYI r'GATC_y
Cuts at: 1 1171 1716 2427 2894 2917 3252 3498 3804
Size: 1170 545 711 467 23 335 246 306
Cuts at: 3804 3816 3902 4954 1
Size: 12 86 1052 449
Fragments arranged by size:
1170 1052 711 545 467 449 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 310 310
Size: 5402
BtgI C'CryG_G
Cuts at: 107 975 1478 1836 1984 1994 2214 107
Size: 868 503 358 148 10 220 3295
Fragments arranged by size:
3295 868 503 358 220 148 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1262 3352 3533 3696 1262
Size: 2090 181 163 2968
Fragments arranged by size:
2968 2090 181 163
BtsI GCAGTG_nn'
Cuts at: 1109 2645 1109
Size: 1536 3866
DraI TTT'AAA
Cuts at: 278 2401 278
Size: 2123 3279
DrdI GACnn_nn'nnGTC
Cuts at: 3108 4452 3108
Size: 1344 4058
EaeI y'GGCC_r
Cuts at: 722 915 1475 2044 2987 3161 3552 3579 4924
Size: 193 560 569 943 174 391 27 1345
Cuts at: 4924 722
Size: 1200
Fragments arranged by size:
1345 1200 943 569 560 391 193 174
27
EagI C'GGCC_G
Cuts at: 2987 2987
Size: 5402
EarI CTCTTCn'nnn_
Cuts at: 225 1010 1337 2117 2264 2288 2342 3425 3635
Size: 785 327 780 147 24 54 1083 210
Cuts at: 3635 225
Size: 1992
Fragments arranged by size:
1992 1083 785 780 327 210 147 54
24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3610 4352 4498 5212 3610
Size: 742 146 714 3800
Fragments arranged by size:
3800 742 714 146
EcoICRI GAG'CTC
Cuts at: 2414 2414
Size: 5402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 706 1709 1991 2068 2245 2302 3227 3659
Size: 480 1003 282 77 177 57 925 432
Cuts at: 3659 4012 4674 226
Size: 353 662 954
Fragments arranged by size:
1003 954 925 662 480 432 353 282
177 77 57
EcoNI CCTnn'n_nnAGG
Cuts at: 1336 1336
Size: 5402
EcoO109I rG'GnC_Cy
Cuts at: 1347 1483 1969 2541 1347
Size: 136 486 572 4208
Fragments arranged by size:
4208 572 486 136
EcoRI G'AATT_C
Cuts at: 2406 2406
Size: 5402
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3421 3421
Size: 5402
FspI TGC'GCA
Cuts at: 965 3183 965
Size: 2218 3184
HaeII r_GCGC'y
Cuts at: 899 2836 3084 4314 5085 899
Size: 1937 248 1230 771 1216
Fragments arranged by size:
1937 1230 1216 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1253 1285 1956 1988 3244 3274 3276 3306 1253
Size: 32 671 32 1256 30 2 30 3349
Fragments arranged by size:
3349 1256 671 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1059 2441 1059
Size: 1382 4020
KasI G'GCGC_C
Cuts at: 895 2832 3080 895
Size: 1937 248 3217
Fragments arranged by size:
3217 1937 248
KpnI G_GTAC'C
Cuts at: 2422 2422
Size: 5402
MfeI C'AATT_G
Cuts at: 1051 1051
Size: 5402
MluI A'CGCG_T
Cuts at: 4835 4835
Size: 5402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 489 4161 4345 489
Size: 3672 184 1546
Fragments arranged by size:
3672 1546 184
MscI TGG'CCA
Cuts at: 724 917 3163 724
Size: 193 2246 2963
Fragments arranged by size:
2963 2246 193
MslI CAynn'nnrTG
Cuts at: 670 1216 1835 3391 3518 670
Size: 546 619 1556 127 2554
Fragments arranged by size:
2554 1556 619 546 127
MspA1I CmG'CkG
Cuts at: 435 1068 1083 2196 2513 2827 3187 3971 4216
Size: 633 15 1113 317 314 360 784 245
Cuts at: 4216 435
Size: 1621
Fragments arranged by size:
1621 1113 784 633 360 317 314 245
15
NaeI GCC'GGC
Cuts at: 3583 3583
Size: 5402
NarI GG'CG_CC
Cuts at: 896 2833 3081 896
Size: 1937 248 3217
Fragments arranged by size:
3217 1937 248
NcoI C'CATG_G
Cuts at: 107 975 1478 1836 107
Size: 868 503 358 3673
Fragments arranged by size:
3673 868 503 358
NgoMIV G'CCGG_C
Cuts at: 3581 3581
Size: 5402
NspI r_CATG'y
Cuts at: 394 2455 2955 4558 394
Size: 2061 500 1603 1238
Fragments arranged by size:
2061 1603 1238 500
PasI CC'CwG_GG
Cuts at: 1021 1021
Size: 5402
PciI A'CATG_T
Cuts at: 4554 4554
Size: 5402
PflMI CCAn_nnn'nTGG
Cuts at: 1068 1068
Size: 5402
PmeI GTTT'AAAC
Cuts at: 2401 2401
Size: 5402
PmlI CAC'GTG
Cuts at: 1291 1291
Size: 5402
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 5370
PpuMI rG'GwC_Cy
Cuts at: 1347 1483 1347
Size: 136 5266
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 760 792 2182 2214 760
Size: 32 1390 32 3948
Fragments arranged by size:
3948 1390 32 32
PstI C_TGCA'G
Cuts at: 2449 3134 2449
Size: 685 4717
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5402
PvuII CAG'CTG
Cuts at: 435 1068 1083 2827 3187 435
Size: 633 15 1744 360 2650
Fragments arranged by size:
2650 1744 633 360 15
RsrII CG'GwC_CG
Cuts at: 3597 3597
Size: 5402
SacI G_AGCT'C
Cuts at: 2416 2416
Size: 5402
SalI G'TCGA_C
Cuts at: 2439 2439
Size: 5402
SapI GCTCTTCn'nnn_
Cuts at: 2342 3425 3635 2342
Size: 1083 210 4109
Fragments arranged by size:
4109 1083 210
SbfI CC_TGCA'GG
Cuts at: 2449 2449
Size: 5402
ScaI AGT'ACT
Cuts at: 774 4919 774
Size: 4145 1257
SfcI C'TryA_G
Cuts at: 33 560 2445 3130 4098 4289 33
Size: 527 1885 685 968 191 1146
Fragments arranged by size:
1885 1146 968 685 527 191
SfoI GGC'GCC
Cuts at: 897 2834 3082 897
Size: 1937 248 3217
Fragments arranged by size:
3217 1937 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5402
SmaI CCC'GGG
Cuts at: 2424 2424
Size: 5402
SmlI C'TyrA_G
Cuts at: 1643 2206 2557 3472 4186 4448 1643
Size: 563 351 915 714 262 2597
Fragments arranged by size:
2597 915 714 563 351 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5402
SphI G_CATG'C
Cuts at: 394 2455 2955 394
Size: 2061 500 2841
Fragments arranged by size:
2841 2061 500
StyI C'CwwG_G
Cuts at: 107 725 975 1478 1836 2072 2536 2890 107
Size: 618 250 503 358 236 464 354 2619
Fragments arranged by size:
2619 618 503 464 358 354 250 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1445 1524 2985 3118 3577 3786 1445
Size: 79 1461 133 459 209 3061
Fragments arranged by size:
3061 1461 459 209 133 79
TatI w'GTAC_w
Cuts at: 247 772 2165 4917 247
Size: 525 1393 2752 732
Fragments arranged by size:
2752 1393 732 525
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 237 269 4919 4951 237
Size: 32 4650 32 688
Fragments arranged by size:
4650 688 32 32
Tth111I GACn'n_nGTC
Cuts at: 2139 3199 2139
Size: 1060 4342
XbaI T'CTAG_A
Cuts at: 2433 2433
Size: 5402
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2897 2897
Size: 5402
XmaI C'CCGG_G
Cuts at: 2422 2422
Size: 5402
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PfoI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI