Restriction map for
pF1KE0700
(Circular) (Six-base) MAPSORT of: pF1KE0700.seq Check: 7703 from: 1 to: 8183
pF1KE0700 8183 bp
With 182 enzymes: SgfI *
March 12, 2010 15:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 312 312
Size: 8183
AccI GT'mk_AC
Cuts at: 3518 5221 3518
Size: 1703 6480
Acc65I G'GTAC_C
Cuts at: 2255 4847 5199 2255
Size: 2592 352 5239
Fragments arranged by size:
5239 2592 352
AclI AA'CG_TT
Cuts at: 8023 8023
Size: 8183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 1243 1438 3241 4338 6008 6440 7187 7455
Size: 1003 195 1803 1097 1670 432 747 268
Cuts at: 7455 240
Size: 968
Fragments arranged by size:
1803 1670 1097 1003 968 747 432 268
195
AflIII A'CryG_T
Cuts at: 308 377 518 4871 4922 5535 6639 7616 308
Size: 69 141 4353 51 613 1104 977 875
Fragments arranged by size:
4353 1104 977 875 613 141 69 51
AgeI A'CCGG_T
Cuts at: 6571 6571
Size: 8183
AhdI GACnn_n'nnGTC
Cuts at: 519 749 4368 519
Size: 230 3619 4334
Fragments arranged by size:
4334 3619 230
AleI CACnn'nnGTG
Cuts at: 1657 2458 4159 1657
Size: 801 1701 5681
Fragments arranged by size:
5681 1701 801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 422 3397 5080 422
Size: 2975 1683 3525
Fragments arranged by size:
3525 2975 1683
AlwNI CAG_nnn'CTG
Cuts at: 2040 2100 2532 4791 5488 7055 2040
Size: 60 432 2259 697 1567 3168
Fragments arranged by size:
3168 2259 1567 697 432 60
ApaI G_GGCC'C
Cuts at: 635 2972 3065 635
Size: 2337 93 5753
Fragments arranged by size:
5753 2337 93
ApaLI G'TGCA_C
Cuts at: 5040 6953 5040
Size: 1913 6270
ApoI r'AATT_y
Cuts at: 5187 7536 7792 5187
Size: 2349 256 5578
Fragments arranged by size:
5578 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8183
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8183
AvaI C'yCGr_G
Cuts at: 158 1389 1897 2185 2907 3419 4711 4757 5203
Size: 1231 508 288 722 512 1292 46 446
Cuts at: 5203 158
Size: 3138
Fragments arranged by size:
3138 1292 1231 722 512 508 446 288
46
BamHI G'GATC_C
Cuts at: 5208 5208
Size: 8183
BanI G'GyrC_C
Cuts at: 406 1549 2255 2571 4136 4657 4847 4907 5199
Size: 1143 706 316 1565 521 190 60 292
Cuts at: 5199 5613 5861 5896 406
Size: 414 248 35 2693
Fragments arranged by size:
2693 1565 1143 706 521 414 316 292
248 190 60 35
BanII G_rGCy'C
Cuts at: 635 1315 1448 1784 1832 2186 2972 3065 3218
Size: 680 133 336 48 354 786 93 153
Cuts at: 3218 4088 4712 4784 5197 6227 635
Size: 870 624 72 413 1030 2591
Fragments arranged by size:
2591 1030 870 786 680 624 413 354
336 153 133 93 72 48
BbeI G_GCGC'C
Cuts at: 4140 4911 5617 5865 4140
Size: 771 706 248 6458
Fragments arranged by size:
6458 771 706 248
BbsI GAAGACnn'nnnn_
Cuts at: 759 1012 1725 3969 4282 759
Size: 253 713 2244 313 4660
Fragments arranged by size:
4660 2244 713 313 253
BbvCI CC'TCA_GC
Cuts at: 1141 2973 4409 1141
Size: 1832 1436 4915
Fragments arranged by size:
4915 1832 1436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2545 2579 2649 2683 2545
Size: 34 70 34 8045
Fragments arranged by size:
8045 70 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 364 3778 6081 6842 8183 364
Size: 3414 2303 761 1341 364
Fragments arranged by size:
3414 2303 1341 761 364
BclI T'GATC_A
Cuts at: 5703 5703
Size: 8183
BfrBI ATG'CAT
Cuts at: 3596 5091 3596
Size: 1495 6688
BglI GCCn_nnn'nGGC
Cuts at: 1513 2770 1513
Size: 1257 6926
BglII A'GATC_T
Cuts at: 3527 5698 7735 3527
Size: 2171 2037 3975
Fragments arranged by size:
3975 2171 2037
BlpI GC'TnA_GC
Cuts at: 1442 1949 3385 3460 4087 4252 5295 1442
Size: 507 1436 75 627 165 1043 4330
Fragments arranged by size:
4330 1436 1043 627 507 165 75
Bme1580I G_kGCm'C
Cuts at: 635 895 1709 2576 2972 3014 3065 3449 5044
Size: 260 814 867 396 42 51 384 1595
Cuts at: 5044 5808 5901 6957 635
Size: 764 93 1056 1861
Fragments arranged by size:
1861 1595 1056 867 814 764 396 384
260 93 51 42
BmgBI CAC'GTC
Cuts at: 2267 2964 3261 3381 4521 4874 2267
Size: 697 297 120 1140 353 5576
Fragments arranged by size:
5576 1140 697 353 297 120
BmrI ACTGGGnnnn_n'
Cuts at: 1971 5395 5476 5811 7955 1971
Size: 3424 81 335 2144 2199
Fragments arranged by size:
3424 2199 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 666 2592 3222 4749 6550 666
Size: 1926 630 1527 1801 2299
Fragments arranged by size:
2299 1926 1801 1527 630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1731 2008 2017 2062 2107 2130 2140 2533 2562
Size: 277 9 45 45 23 10 393 29
Cuts at: 2562 3559 3707 4218 4264 4330 4368 4450 4882
Size: 997 148 511 46 66 38 82 432
Cuts at: 4882 5133 1731
Size: 251 4781
Fragments arranged by size:
4781 997 511 432 393 277 251 148
82 66 46 45 45 38 29 23
10 9
Bpu10I CC'TnA_GC
Cuts at: 1132 1141 2521 2677 2973 4409 1132
Size: 9 1380 156 296 1436 4906
Fragments arranged by size:
4906 1436 1380 296 156 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1059 1119 2290 4999 5359 6238 6730 7028
Size: 173 60 1171 2709 360 879 492 298
Cuts at: 7028 886
Size: 2041
Fragments arranged by size:
2709 2041 1171 879 492 360 298 173
60
BsaI GGTCTCn'nnnn_
Cuts at: 736 799 1120 1447 2612 3721 4306 7887 736
Size: 63 321 327 1165 1109 585 3581 1032
Fragments arranged by size:
3581 1165 1109 1032 585 327 321 63
BsaAI yAC'GTr
Cuts at: 639 4101 5536 639
Size: 3462 1435 3286
Fragments arranged by size:
3462 3286 1435
BsaBI GATnn'nnATC
Cuts at: 6 5697 7734 6
Size: 5691 2037 455
Fragments arranged by size:
5691 2037 455
BsaHI Gr'CG_yC
Cuts at: 244 1942 2813 3133 4137 4908 5614 5862 6564
Size: 1698 871 320 1004 771 706 248 702
Cuts at: 6564 8060 244
Size: 1496 367
Fragments arranged by size:
1698 1496 1004 871 771 706 702 367
320 248
BsaWI w'CCGG_w
Cuts at: 976 1490 1736 1883 2240 3781 3809 5372 5893
Size: 514 246 147 357 1541 28 1563 521
Cuts at: 5893 6571 6845 6992 7403 976
Size: 678 274 147 411 1756
Fragments arranged by size:
1756 1563 1541 678 521 514 411 357
274 246 147 147 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2035 2065 4200 4230 4327 4357 2035
Size: 30 2135 30 97 30 5861
Fragments arranged by size:
5861 2135 97 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 1039 1414 1477 1735 2083 2185 2188 2191
Size: 681 375 63 258 348 102 3 3
Cuts at: 2191 2194 3016 3601 4195 4371 4383 4611 358
Size: 3 822 585 594 176 12 228 3930
Fragments arranged by size:
3930 822 681 594 585 375 348 258
228 176 102 63 12 3 3 3
BseYI C'CCAG_C
Cuts at: 627 856 1831 1973 3795 3941 4319 4784 4880
Size: 229 975 142 1822 146 378 465 96
Cuts at: 4880 5608 6366 6943 7439 627
Size: 728 758 577 496 1371
Fragments arranged by size:
1822 1371 975 758 728 577 496 465
378 229 146 142 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2978 3921 4837 2978
Size: 943 916 6324
Fragments arranged by size:
6324 943 916
BsiEI CG_ry'CG
Cuts at: 104 2559 3566 4832 5725 5771 6979 104
Size: 2455 1007 1266 893 46 1208 1308
Fragments arranged by size:
2455 1308 1266 1208 1007 893 46
BsiHKAI G_wGCw'C
Cuts at: 620 1448 1727 3386 4088 4964 5044 5197 5975
Size: 828 279 1659 702 876 80 153 778
Cuts at: 5975 6165 6957 620
Size: 190 792 1846
Fragments arranged by size:
1846 1659 876 828 792 778 702 279
190 153 80
BsmBI CGTCTCn'nnnn_
Cuts at: 235 1933 2273 2428 235
Size: 1698 340 155 5990
Fragments arranged by size:
5990 1698 340 155
Bsp1286I G_dGCh'C
Cuts at: 620 635 895 1315 1448 1709 1727 1784 1832
Size: 15 260 420 133 261 18 57 48
Cuts at: 1832 2186 2576 2972 3014 3065 3218 3386 3449
Size: 354 390 396 42 51 153 168 63
Cuts at: 3449 4088 4712 4784 4964 5044 5197 5808 5901
Size: 639 624 72 180 80 153 611 93
Cuts at: 5901 5975 6165 6227 6957 620
Size: 74 190 62 730 1846
Fragments arranged by size:
1846 730 639 624 611 420 396 390
354 261 260 190 180 168 153 153
133 93 80 74 72 63 62 57
51 48 42 18 15
BspEI T'CCGG_A
Cuts at: 976 1490 1736 2240 3781 7403 976
Size: 514 246 504 1541 3622 1756
Fragments arranged by size:
3622 1756 1541 514 504 246
BspHI T'CATG_A
Cuts at: 7359 7359
Size: 8183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 312 2090 2288 2411 2693 3518 4298 4859 5233
Size: 1778 198 123 282 825 780 561 374
Cuts at: 5233 5447 5749 6130 312
Size: 214 302 381 2365
Fragments arranged by size:
2365 1778 825 780 561 381 374 302
282 214 198 123
BsrBI CCG'CTC
Cuts at: 50 867 2064 2211 2906 4401 4953 6475 6529
Size: 817 1197 147 695 1495 552 1522 54
Cuts at: 6529 8013 8178 50
Size: 1484 165 55
Fragments arranged by size:
1522 1495 1484 1197 817 695 552 165
147 55 54
BsrDI GCAATG_nn'
Cuts at: 1182 6095 1182
Size: 4913 3270
BsrFI r'CCGG_y
Cuts at: 409 2611 3945 4133 6181 6362 6571 409
Size: 2202 1334 188 2048 181 209 2021
Fragments arranged by size:
2202 2048 2021 1334 209 188 181
BsrGI T'GTAC_A
Cuts at: 4925 4925
Size: 8183
BssSI C'ACGA_G
Cuts at: 1369 6454 6812 1369
Size: 5085 358 2740
Fragments arranged by size:
5085 2740 358
BstAPI GCAn_nnn'nTGC
Cuts at: 624 3593 4290 5010 5560 624
Size: 2969 697 720 550 3247
Fragments arranged by size:
3247 2969 720 697 550
BstBI TT'CG_AA
Cuts at: 6544 6544
Size: 8183
BstEII G'GTnAC_C
Cuts at: 1233 1770 2607 3282 3876 1233
Size: 537 837 675 594 5540
Fragments arranged by size:
5540 837 675 594 537
BstXI CCAn_nnnn'nTGG
Cuts at: 720 1270 1299 1494 2328 720
Size: 550 29 195 834 6575
Fragments arranged by size:
6575 834 550 195 29
BstYI r'GATC_y
Cuts at: 1 1166 3527 4814 5208 5675 5698 6033 6279
Size: 1165 2361 1287 394 467 23 335 246
Cuts at: 6279 7291 7377 7389 7735 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2361 1287 1165 1012 467 449 394 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 293 3394 4669 293
Size: 3101 1275 3807
Fragments arranged by size:
3807 3101 1275
BtgI C'CryG_G
Cuts at: 107 530 719 818 878 2465 2888 3566 3579
Size: 423 189 99 60 1587 423 678 13
Cuts at: 3579 4374 4946 5052 107
Size: 795 572 106 3238
Fragments arranged by size:
3238 1587 795 678 572 423 423 189
106 99 60 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3255 6133 6314 6477 3255
Size: 2878 181 163 4961
Fragments arranged by size:
4961 2878 181 163
BtsI GCAGTG_nn'
Cuts at: 622 1725 3014 3281 3495 4041 4973 5426 622
Size: 1103 1289 267 214 546 932 453 3379
Fragments arranged by size:
3379 1289 1103 932 546 453 267 214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 198 233 198
Size: 35 8148
DraI TTT'AAA
Cuts at: 5182 5182
Size: 8183
DraIII CAC_nnn'GTG
Cuts at: 2715 2715
Size: 8183
DrdI GACnn_nn'nnGTC
Cuts at: 1804 3514 3735 5136 5889 6747 1804
Size: 1710 221 1401 753 858 3240
Fragments arranged by size:
3240 1710 1401 858 753 221
EaeI y'GGCC_r
Cuts at: 110 725 1514 1529 2462 2762 3339 3563 4460
Size: 615 789 15 933 300 577 224 897
Cuts at: 4460 4604 4693 4970 5123 5768 5942 6333 6360
Size: 144 89 277 153 645 174 391 27
Cuts at: 6360 7705 110
Size: 1345 588
Fragments arranged by size:
1345 933 897 789 645 615 588 577
391 300 277 224 174 153 144 89
27 15
EagI C'GGCC_G
Cuts at: 3563 5768 3563
Size: 2205 5978
EarI CTCTTCn'nnn_
Cuts at: 490 2783 2918 3783 6206 6416 490
Size: 2293 135 865 2423 210 2257
Fragments arranged by size:
2423 2293 2257 865 210 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 133 945 1491 2630 2806 3079 6391 6701 6847
Size: 812 546 1139 176 273 3312 310 146
Cuts at: 6847 7993 133
Size: 1146 323
Fragments arranged by size:
3312 1146 1139 812 546 323 310 273
176 146
EcoICRI GAG'CTC
Cuts at: 1446 4086 5195 1446
Size: 2640 1109 4434
Fragments arranged by size:
4434 2640 1109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 1243 1438 1731 2008 2017 2062 2107 2130
Size: 1003 195 293 277 9 45 45 23
Cuts at: 2130 2140 2533 2562 3241 3559 3707 4218 4264
Size: 10 393 29 679 318 148 511 46
Cuts at: 4264 4330 4338 4368 4450 4882 5133 6008 6440
Size: 66 8 30 82 432 251 875 432
Cuts at: 6440 7187 7455 240
Size: 747 268 968
Fragments arranged by size:
1003 968 875 747 679 511 432 432
393 318 293 277 268 251 195 148
82 66 46 45 45 30 29 23
10 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 904 943 1600 2308 3373 3616 4261 4264 5065
Size: 39 657 708 1065 243 645 3 801
Cuts at: 5065 904
Size: 4022
Fragments arranged by size:
4022 1065 801 708 657 645 243 39
3
EcoO109I rG'GnC_Cy
Cuts at: 480 631 632 645 687 1194 1248 1605 2277
Size: 151 1 13 42 507 54 357 672
Cuts at: 2277 2663 2793 2968 2969 3062 3238 3398 3487
Size: 386 130 175 1 93 176 160 89
Cuts at: 3487 3602 4202 4578 4673 5322 480
Size: 115 600 376 95 649 3341
Fragments arranged by size:
3341 672 649 600 507 386 376 357
176 175 160 151 130 115 95 93
89 54 42 13 1 1
EcoRI G'AATT_C
Cuts at: 5187 5187
Size: 8183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6202 6202
Size: 8183
FspI TGC'GCA
Cuts at: 5964 5964
Size: 8183
HaeII r_GCGC'y
Cuts at: 795 2230 4134 4140 4391 4791 4893 4911 5617
Size: 1435 1904 6 251 400 102 18 706
Cuts at: 5617 5865 6887 7866 795
Size: 248 1022 979 1112
Fragments arranged by size:
1904 1435 1112 1022 979 706 400 251
248 102 18 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 472 504 505 537 735 767 2791 2823 3881
Size: 32 1 32 198 32 2024 32 1058
Cuts at: 3881 3913 4200 4232 4327 4354 4359 4386 4578
Size: 32 287 32 95 27 5 27 192
Cuts at: 4578 4610 6025 6055 6057 6087 472
Size: 32 1415 30 2 30 2568
Fragments arranged by size:
2568 2024 1415 1058 287 198 192 95
32 32 32 32 32 32 32 30
30 27 27 5 2 1
HincII GTy'rAC
Cuts at: 222 1040 1593 2988 5222 222
Size: 818 553 1395 2234 3183
Fragments arranged by size:
3183 2234 1395 818 553
HindIII A'AGCT_T
Cuts at: 252 252
Size: 8183
HpaI GTT'AAC
Cuts at: 222 222
Size: 8183
KasI G'GCGC_C
Cuts at: 4136 4907 5613 5861 4136
Size: 771 706 248 6458
Fragments arranged by size:
6458 771 706 248
KpnI G_GTAC'C
Cuts at: 2259 4851 5203 2259
Size: 2592 352 5239
Fragments arranged by size:
5239 2592 352
MluI A'CGCG_T
Cuts at: 308 7616 308
Size: 7308 875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 6854 7038 6854
Size: 184 7999
MscI TGG'CCA
Cuts at: 1531 3341 4606 4972 5944 1531
Size: 1810 1265 366 972 3770
Fragments arranged by size:
3770 1810 1265 972 366
MslI CAynn'nnrTG
Cuts at: 193 427 733 1285 1492 1657 1861 2005 2326
Size: 234 306 552 207 165 204 144 321
Cuts at: 2326 2458 3274 4159 6172 6299 193
Size: 132 816 885 2013 127 2077
Fragments arranged by size:
2077 2013 885 816 552 321 306 234
207 204 165 144 132 127
MspA1I CmG'CkG
Cuts at: 1172 1611 1948 2097 3677 4323 4452 5054 5294
Size: 439 337 149 1580 646 129 602 240
Cuts at: 5294 5608 5968 6981 7226 1172
Size: 314 360 1013 245 2129
Fragments arranged by size:
2129 1580 1013 646 602 439 360 337
314 245 240 149 129
NaeI GCC'GGC
Cuts at: 411 3947 4135 6364 411
Size: 3536 188 2229 2230
Fragments arranged by size:
3536 2230 2229 188
NarI GG'CG_CC
Cuts at: 4137 4908 5614 5862 4137
Size: 771 706 248 6458
Fragments arranged by size:
6458 771 706 248
NcoI C'CATG_G
Cuts at: 107 4374 107
Size: 4267 3916
NgoMIV G'CCGG_C
Cuts at: 409 3945 4133 6362 409
Size: 3536 188 2229 2230
Fragments arranged by size:
3536 2230 2229 188
NsiI A_TGCA'T
Cuts at: 3598 5093 3598
Size: 1495 6688
NspI r_CATG'y
Cuts at: 361 522 3591 4926 5091 5236 5736 6643 361
Size: 161 3069 1335 165 145 500 907 1901
Fragments arranged by size:
3069 1901 1335 907 500 165 161 145
PasI CC'CwG_GG
Cuts at: 939 1263 4120 939
Size: 324 2857 5002
Fragments arranged by size:
5002 2857 324
PciI A'CATG_T
Cuts at: 518 4922 6639 518
Size: 4404 1717 2062
Fragments arranged by size:
4404 2062 1717
PflMI CCAn_nnn'nTGG
Cuts at: 2040 2040
Size: 8183
PfoI T'CCnGG_A
Cuts at: 683 1052 2033 2474 3483 4148 4331 4574 4808
Size: 369 981 441 1009 665 183 243 234
Cuts at: 4808 4859 683
Size: 51 4007
Fragments arranged by size:
4007 1009 981 665 441 369 243 234
183 51
PmeI GTTT'AAAC
Cuts at: 5182 5182
Size: 8183
PmlI CAC'GTG
Cuts at: 4101 4101
Size: 8183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7322 7354 7322
Size: 32 8151
PpuMI rG'GwC_Cy
Cuts at: 480 645 687 1194 1248 1605 2277 3487 3602
Size: 165 42 507 54 357 672 1210 115
Cuts at: 3602 4578 4673 480
Size: 976 95 3990
Fragments arranged by size:
3990 1210 976 672 507 357 165 115
95 54 42
PspOMI G'GGCC_C
Cuts at: 631 2968 3061 631
Size: 2337 93 5753
Fragments arranged by size:
5753 2337 93
PstI C_TGCA'G
Cuts at: 356 1110 2132 2568 3131 4265 5069 5230 5915
Size: 754 1022 436 563 1134 804 161 685
Cuts at: 5915 356
Size: 2624
Fragments arranged by size:
2624 1134 1022 804 754 685 563 436
161
PvuI CG_AT'CG
Cuts at: 104 4832 104
Size: 4728 3455
PvuII CAG'CTG
Cuts at: 1948 5608 5968 1948
Size: 3660 360 4163
Fragments arranged by size:
4163 3660 360
RsrII CG'GwC_CG
Cuts at: 6378 6378
Size: 8183
SacI G_AGCT'C
Cuts at: 1448 4088 5197 1448
Size: 2640 1109 4434
Fragments arranged by size:
4434 2640 1109
SacII CC_GC'GG
Cuts at: 5055 5055
Size: 8183
SalI G'TCGA_C
Cuts at: 5220 5220
Size: 8183
SanDI GG'GwC_CC
Cuts at: 3487 4578 3487
Size: 1091 7092
SapI GCTCTTCn'nnn_
Cuts at: 2918 6206 6416 2918
Size: 3288 210 4685
Fragments arranged by size:
4685 3288 210
SbfI CC_TGCA'GG
Cuts at: 3131 4265 5230 3131
Size: 1134 965 6084
Fragments arranged by size:
6084 1134 965
ScaI AGT'ACT
Cuts at: 7700 7700
Size: 8183
SfcI C'TryA_G
Cuts at: 33 352 1106 2128 2564 3127 4261 5065 5226
Size: 319 754 1022 436 563 1134 804 161
Cuts at: 5226 5911 6904 7095 33
Size: 685 993 191 1121
Fragments arranged by size:
1134 1121 1022 993 804 754 685 563
436 319 191 161
SfiI GGCCn_nnn'nGGCC
Cuts at: 2770 2770
Size: 8183
SfoI GGC'GCC
Cuts at: 4138 4909 5615 5863 4138
Size: 771 706 248 6458
Fragments arranged by size:
6458 771 706 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8183
SgrAI Cr'CCGG_yG
Cuts at: 4133 4133
Size: 8183
SmaI CCC'GGG
Cuts at: 160 1391 4759 5205 160
Size: 1231 3368 446 3138
Fragments arranged by size:
3368 3138 1231 446
SmlI C'TyrA_G
Cuts at: 901 1074 1098 2305 5014 5338 6253 6745 7007
Size: 173 24 1207 2709 324 915 492 262
Cuts at: 7007 901
Size: 2077
Fragments arranged by size:
2709 2077 1207 915 492 324 262 173
24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8183
SphI G_CATG'C
Cuts at: 361 3591 5236 5736 361
Size: 3230 1645 500 2808
Fragments arranged by size:
3230 2808 1645 500
SrfI GCCC'GGGC
Cuts at: 4759 4759
Size: 8183
StuI AGG'CCT
Cuts at: 3023 3202 3023
Size: 179 8004
StyI C'CwwG_G
Cuts at: 107 236 887 1553 1775 3959 4374 5317 5671
Size: 129 651 666 222 2184 415 943 354
Cuts at: 5671 107
Size: 2619
Fragments arranged by size:
2619 2184 943 666 651 415 354 222
129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 635 799 1513 2444 2512 3745 3809 3955 5037
Size: 164 714 931 68 1233 64 146 1082
Cuts at: 5037 5766 5899 6358 6567 635
Size: 729 133 459 209 2251
Fragments arranged by size:
2251 1233 1082 931 729 714 459 209
164 146 133 68 64
TatI w'GTAC_w
Cuts at: 3302 4505 4925 4988 7698 3302
Size: 1203 420 63 2710 3787
Fragments arranged by size:
3787 2710 1203 420 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 362 394 2269 2301 4219 4251 7700 7732 362
Size: 32 1875 32 1918 32 3449 32 813
Fragments arranged by size:
3449 1918 1875 813 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3288 5980 3288
Size: 2692 5491
XbaI T'CTAG_A
Cuts at: 5214 5214
Size: 8183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1299 2082 2463 4893 4980 5678 1299
Size: 783 381 2430 87 698 3804
Fragments arranged by size:
3804 2430 783 698 381 87
XmaI C'CCGG_G
Cuts at: 158 1389 4757 5203 158
Size: 1231 3368 446 3138
Fragments arranged by size:
3368 3138 1231 446
XmnI GAAnn'nnTTC
Cuts at: 2749 2927 2749
Size: 178 8005
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BaeI BmtI
BsiWI BsmI BssHII BstZ17I ClaI EcoRV FseI FspAI
MfeI NdeI NheI NotI NruI PacI PshAI PsiI
PspXI PsrI SexAI SnaBI SspI SwaI XhoI ZraI