Restriction map for
pF1KE0706
(Circular) (Six-base) MAPSORT of: pF1KE0706.seq Check: 8096 from: 1 to: 4334
pF1KE0706 4334 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1372 1372
Size: 4334
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2591 3338 3606 2159
Size: 432 747 268 2887
Fragments arranged by size:
2887 747 432 268
AflIII A'CryG_T
Cuts at: 1326 1686 2790 3767 1326
Size: 360 1104 977 1893
Fragments arranged by size:
1893 1104 977 360
AgeI A'CCGG_T
Cuts at: 2722 2722
Size: 4334
AleI CACnn'nnGTG
Cuts at: 476 476
Size: 4334
AlwNI CAG_nnn'CTG
Cuts at: 1639 3206 1639
Size: 1567 2767
ApaI G_GGCC'C
Cuts at: 1258 1258
Size: 4334
ApaLI G'TGCA_C
Cuts at: 3104 3104
Size: 4334
ApoI r'AATT_y
Cuts at: 277 464 1338 3687 3943 277
Size: 187 874 2349 256 668
Fragments arranged by size:
2349 874 668 256 187
AseI AT'TA_AT
Cuts at: 20 20
Size: 4334
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 117 257 368 566 677 1054 1354 117
Size: 140 111 198 111 377 300 3097
Fragments arranged by size:
3097 377 300 198 140 111 111
BamHI G'GATC_C
Cuts at: 1359 1359
Size: 4334
BanI G'GyrC_C
Cuts at: 121 712 816 961 1350 1764 2012 2047 121
Size: 591 104 145 389 414 248 35 2408
Fragments arranged by size:
2408 591 414 389 248 145 104 35
BanII G_rGCy'C
Cuts at: 494 873 1258 1348 2378 494
Size: 379 385 90 1030 2450
Fragments arranged by size:
2450 1030 385 379 90
BbeI G_GCGC'C
Cuts at: 125 1768 2016 125
Size: 1643 248 2443
Fragments arranged by size:
2443 1643 248
BciVI GTATCCnnnnn_n'
Cuts at: 2232 2993 4334 2232
Size: 761 1341 2232
Fragments arranged by size:
2232 1341 761
BclI T'GATC_A
Cuts at: 1854 1854
Size: 4334
BfrBI ATG'CAT
Cuts at: 627 627
Size: 4334
BglI GCCn_nnn'nGGC
Cuts at: 783 783
Size: 4334
BglII A'GATC_T
Cuts at: 1849 3886 1849
Size: 2037 2297
BlpI GC'TnA_GC
Cuts at: 671 1127 1446 671
Size: 456 319 3559
Fragments arranged by size:
3559 456 319
Bme1580I G_kGCm'C
Cuts at: 743 966 1258 1959 2052 3108 743
Size: 223 292 701 93 1056 1969
Fragments arranged by size:
1969 1056 701 292 223 93
BmrI ACTGGGnnnn_n'
Cuts at: 1546 1627 1962 4106 1546
Size: 81 335 2144 1774
Fragments arranged by size:
2144 1774 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 4334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 941 183
Size: 758 3576
Bpu10I CC'TnA_GC
Cuts at: 493 493
Size: 4334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1194 1510 2389 2881 3179 1194
Size: 316 879 492 298 2349
Fragments arranged by size:
2349 879 492 316 298
BsaI GGTCTCn'nnnn_
Cuts at: 4038 4038
Size: 4334
BsaAI yAC'GTr
Cuts at: 782 1687 782
Size: 905 3429
BsaBI GATnn'nnATC
Cuts at: 6 1848 3885 6
Size: 1842 2037 455
Fragments arranged by size:
2037 1842 455
BsaHI Gr'CG_yC
Cuts at: 122 548 1765 2013 2715 4211 122
Size: 426 1217 248 702 1496 245
Fragments arranged by size:
1496 1217 702 426 248 245
BsaWI w'CCGG_w
Cuts at: 1523 2044 2722 2996 3143 3554 1523
Size: 521 678 274 147 411 2303
Fragments arranged by size:
2303 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 200 230 200
Size: 30 4304
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 881 1028 881
Size: 147 4187
BseYI C'CCAG_C
Cuts at: 758 1204 1759 2517 3094 3590 758
Size: 446 555 758 577 496 1502
Fragments arranged by size:
1502 758 577 555 496 446
BsiEI CG_ry'CG
Cuts at: 104 397 1876 1922 3130 104
Size: 293 1479 46 1208 1308
Fragments arranged by size:
1479 1308 1208 293 46
BsiHKAI G_wGCw'C
Cuts at: 169 494 1348 2126 2316 3108 169
Size: 325 854 778 190 792 1395
Fragments arranged by size:
1395 854 792 778 325 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1094 1094
Size: 4334
Bsp1286I G_dGCh'C
Cuts at: 169 494 743 873 966 1258 1348 1959 2052
Size: 325 249 130 93 292 90 611 93
Cuts at: 2052 2126 2316 2378 3108 169
Size: 74 190 62 730 1395
Fragments arranged by size:
1395 730 611 325 292 249 190 130
93 93 90 74 62
BspEI T'CCGG_A
Cuts at: 3554 3554
Size: 4334
BspHI T'CATG_A
Cuts at: 230 536 3510 230
Size: 306 2974 1054
Fragments arranged by size:
2974 1054 306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1147 1384 1598 1900 2281 1147
Size: 237 214 302 381 3200
Fragments arranged by size:
3200 381 302 237 214
BsrBI CCG'CTC
Cuts at: 50 2626 2680 4164 4329 50
Size: 2576 54 1484 165 55
Fragments arranged by size:
2576 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2246 2246
Size: 4334
BsrFI r'CCGG_y
Cuts at: 819 1264 2332 2513 2722 819
Size: 445 1068 181 209 2431
Fragments arranged by size:
2431 1068 445 209 181
BssSI C'ACGA_G
Cuts at: 2605 2963 2605
Size: 358 3976
BstAPI GCAn_nnn'nTGC
Cuts at: 1711 1711
Size: 4334
BstBI TT'CG_AA
Cuts at: 2695 2695
Size: 4334
BstYI r'GATC_y
Cuts at: 1 1359 1826 1849 2184 2430 3442 3528 3540
Size: 1358 467 23 335 246 1012 86 12
Cuts at: 3540 3886 1
Size: 346 449
Fragments arranged by size:
1358 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 950 950
Size: 4334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2284 2465 2628 2284
Size: 181 163 3990
Fragments arranged by size:
3990 181 163
BtsI GCAGTG_nn'
Cuts at: 1577 1577
Size: 4334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 278 313 793 828 278
Size: 35 480 35 3784
Fragments arranged by size:
3784 480 35 35
DraI TTT'AAA
Cuts at: 282 1333 282
Size: 1051 3283
DraIII CAC_nnn'GTG
Cuts at: 1302 1302
Size: 4334
DrdI GACnn_nn'nnGTC
Cuts at: 616 2040 2898 616
Size: 1424 858 2052
Fragments arranged by size:
2052 1424 858
EaeI y'GGCC_r
Cuts at: 311 336 394 1919 2093 2484 2511 3856 311
Size: 25 58 1525 174 391 27 1345 789
Fragments arranged by size:
1525 1345 789 391 174 58 27 25
EagI C'GGCC_G
Cuts at: 394 1919 394
Size: 1525 2809
EarI CTCTTCn'nnn_
Cuts at: 148 858 2357 2567 148
Size: 710 1499 210 1915
Fragments arranged by size:
1915 1499 710 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2542 2852 2998 4144 2542
Size: 310 146 1146 2732
Fragments arranged by size:
2732 1146 310 146
EcoICRI GAG'CTC
Cuts at: 492 1346 492
Size: 854 3480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 941 2159 2591 3338 3606 183
Size: 758 1218 432 747 268 911
Fragments arranged by size:
1218 911 758 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 920 920
Size: 4334
EcoO109I rG'GnC_Cy
Cuts at: 418 563 1231 1254 1255 1473 418
Size: 145 668 23 1 218 3279
Fragments arranged by size:
3279 668 218 145 23 1
EcoRI G'AATT_C
Cuts at: 464 1338 464
Size: 874 3460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2353 2353
Size: 4334
FspI TGC'GCA
Cuts at: 187 2115 187
Size: 1928 2406
HaeII r_GCGC'y
Cuts at: 125 700 1768 2016 3038 4017 125
Size: 575 1068 248 1022 979 442
Fragments arranged by size:
1068 1022 979 575 442 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 586 618 2176 2206 2208 2238 586
Size: 32 1558 30 2 30 2682
Fragments arranged by size:
2682 1558 32 30 30 2
HincII GTy'rAC
Cuts at: 621 1373 621
Size: 752 3582
KasI G'GCGC_C
Cuts at: 121 1764 2012 121
Size: 1643 248 2443
Fragments arranged by size:
2443 1643 248
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MfeI C'AATT_G
Cuts at: 474 474
Size: 4334
MluI A'CGCG_T
Cuts at: 1326 3767 1326
Size: 2441 1893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3005 3189 3005
Size: 184 4150
MscI TGG'CCA
Cuts at: 313 2095 313
Size: 1782 2552
MslI CAynn'nnrTG
Cuts at: 476 2323 2450 476
Size: 1847 127 2360
Fragments arranged by size:
2360 1847 127
MspA1I CmG'CkG
Cuts at: 1274 1445 1759 2119 3132 3377 1274
Size: 171 314 360 1013 245 2231
Fragments arranged by size:
2231 1013 360 314 245 171
NaeI GCC'GGC
Cuts at: 821 1266 2515 821
Size: 445 1249 2640
Fragments arranged by size:
2640 1249 445
NarI GG'CG_CC
Cuts at: 122 1765 2013 122
Size: 1643 248 2443
Fragments arranged by size:
2443 1643 248
NgoMIV G'CCGG_C
Cuts at: 819 1264 2513 819
Size: 445 1249 2640
Fragments arranged by size:
2640 1249 445
NsiI A_TGCA'T
Cuts at: 629 629
Size: 4334
NspI r_CATG'y
Cuts at: 627 1387 1887 2794 627
Size: 760 500 907 2167
Fragments arranged by size:
2167 907 760 500
PasI CC'CwG_GG
Cuts at: 939 939
Size: 4334
PciI A'CATG_T
Cuts at: 2790 2790
Size: 4334
PflMI CCAn_nnn'nTGG
Cuts at: 722 758 722
Size: 36 4298
PfoI T'CCnGG_A
Cuts at: 918 918
Size: 4334
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PmlI CAC'GTG
Cuts at: 782 782
Size: 4334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4302
PpuMI rG'GwC_Cy
Cuts at: 418 1231 418
Size: 813 3521
PspOMI G'GGCC_C
Cuts at: 1254 1254
Size: 4334
PstI C_TGCA'G
Cuts at: 861 1381 2066 861
Size: 520 685 3129
Fragments arranged by size:
3129 685 520
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 1759 2119 1759
Size: 360 3974
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 494 1348 494
Size: 854 3480
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SanDI GG'GwC_CC
Cuts at: 1231 1231
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 148 2357 2567 148
Size: 2209 210 1915
Fragments arranged by size:
2209 1915 210
SbfI CC_TGCA'GG
Cuts at: 861 1381 861
Size: 520 3814
ScaI AGT'ACT
Cuts at: 3851 3851
Size: 4334
SfcI C'TryA_G
Cuts at: 33 857 1377 2062 3055 3246 33
Size: 824 520 685 993 191 1121
Fragments arranged by size:
1121 993 824 685 520 191
SfoI GGC'GCC
Cuts at: 123 1766 2014 123
Size: 1643 248 2443
Fragments arranged by size:
2443 1643 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SgrAI Cr'CCGG_yG
Cuts at: 1264 1264
Size: 4334
SmaI CCC'GGG
Cuts at: 370 1056 1356 370
Size: 686 300 3348
Fragments arranged by size:
3348 686 300
SmlI C'TyrA_G
Cuts at: 257 1173 1489 2404 2896 3158 257
Size: 916 316 915 492 262 1433
Fragments arranged by size:
1433 916 915 492 316 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4334
SphI G_CATG'C
Cuts at: 1387 1887 1387
Size: 500 3834
StyI C'CwwG_G
Cuts at: 1468 1822 1468
Size: 354 3980
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 804 1917 2050 2509 2718 804
Size: 1113 133 459 209 2420
Fragments arranged by size:
2420 1113 459 209 133
TatI w'GTAC_w
Cuts at: 3849 3849
Size: 4334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 800 832 3851 3883 800
Size: 32 3019 32 1251
Fragments arranged by size:
3019 1251 32 32
Tth111I GACn'n_nGTC
Cuts at: 2131 2131
Size: 4334
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1829 1829
Size: 4334
XhoI C'TCGA_G
Cuts at: 257 257
Size: 4334
XmaI C'CCGG_G
Cuts at: 368 1054 1354 368
Size: 686 300 3348
Fragments arranged by size:
3348 686 300
XmnI GAAnn'nnTTC
Cuts at: 142 142
Size: 4334
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BmgBI BmtI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I BtgI
ClaI EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI PacI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SnaBI SrfI SspI StuI SwaI
ZraI