Restriction map for
pF1KE0717
(Circular) (Six-base) MAPSORT of: pF1KE0717.seq Check: 6672 from: 1 to: 4028
pF1KE0717 4028 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1066 1066
Size: 4028
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1853 2285 3032 3300 364
Size: 1489 432 747 268 1092
Fragments arranged by size:
1489 1092 747 432 268
AfeI AGC'GCT
Cuts at: 244 244
Size: 4028
AflIII A'CryG_T
Cuts at: 1380 2484 3461 1380
Size: 1104 977 1947
Fragments arranged by size:
1947 1104 977
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AhdI GACnn_n'nnGTC
Cuts at: 507 507
Size: 4028
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 718 750 718
Size: 32 3996
AlwNI CAG_nnn'CTG
Cuts at: 947 1333 2900 947
Size: 386 1567 2075
Fragments arranged by size:
2075 1567 386
ApaI G_GGCC'C
Cuts at: 908 908
Size: 4028
ApaLI G'TGCA_C
Cuts at: 2798 2798
Size: 4028
ApoI r'AATT_y
Cuts at: 114 1032 3381 3637 114
Size: 918 2349 256 505
Fragments arranged by size:
2349 918 505 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 359 680 1048 359
Size: 321 368 3339
Fragments arranged by size:
3339 368 321
BamHI G'GATC_C
Cuts at: 1053 1053
Size: 4028
BanI G'GyrC_C
Cuts at: 382 472 1044 1458 1706 1741 382
Size: 90 572 414 248 35 2669
Fragments arranged by size:
2669 572 414 248 90 35
BanII G_rGCy'C
Cuts at: 908 1042 2072 908
Size: 134 1030 2864
Fragments arranged by size:
2864 1030 134
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BbvCI CC'TCA_GC
Cuts at: 437 437
Size: 4028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 478 512 1002 1036 478
Size: 34 490 34 3470
Fragments arranged by size:
3470 490 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4028
BglI GCCn_nnn'nGGC
Cuts at: 179 301 695 179
Size: 122 394 3512
Fragments arranged by size:
3512 394 122
BglII A'GATC_T
Cuts at: 1543 3580 1543
Size: 2037 1991
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 387 908 1653 1746 2802 387
Size: 521 745 93 1056 1613
Fragments arranged by size:
1613 1056 745 521 93
BmgBI CAC'GTC
Cuts at: 432 432
Size: 4028
BmrI ACTGGGnnnn_n'
Cuts at: 1240 1321 1656 3800 1240
Size: 81 335 2144 1468
Fragments arranged by size:
2144 1468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 477 2395 477
Size: 1918 2110
Bpu10I CC'TnA_GC
Cuts at: 437 437
Size: 4028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 2083 2575 2873 1204
Size: 879 492 298 2359
Fragments arranged by size:
2359 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 713 3732 713
Size: 3019 1009
BsaAI yAC'GTr
Cuts at: 1381 1381
Size: 4028
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 293 1459 1707 2409 3905 293
Size: 1166 248 702 1496 416
Fragments arranged by size:
1496 1166 702 416 248
BsaWI w'CCGG_w
Cuts at: 649 1217 1738 2416 2690 2837 3248 649
Size: 568 521 678 274 147 411 1429
Fragments arranged by size:
1429 678 568 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 298 298
Size: 4028
BseYI C'CCAG_C
Cuts at: 947 1453 2211 2788 3284 947
Size: 506 758 577 496 1691
Fragments arranged by size:
1691 758 577 506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 175
Size: 4028
BsiEI CG_ry'CG
Cuts at: 104 690 699 957 1570 1616 2824 104
Size: 586 9 258 613 46 1208 1308
Fragments arranged by size:
1308 1208 613 586 258 46 9
BsiHKAI G_wGCw'C
Cuts at: 458 1042 1820 2010 2802 458
Size: 584 778 190 792 1684
Fragments arranged by size:
1684 792 778 584 190
BsmBI CGTCTCn'nnnn_
Cuts at: 422 422
Size: 4028
Bsp1286I G_dGCh'C
Cuts at: 387 458 908 1042 1653 1746 1820 2010 2072
Size: 71 450 134 611 93 74 190 62
Cuts at: 2072 2802 387
Size: 730 1613
Fragments arranged by size:
1613 730 611 450 190 134 93 74
71 62
BspEI T'CCGG_A
Cuts at: 3248 3248
Size: 4028
BspHI T'CATG_A
Cuts at: 3204 3204
Size: 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1078 1292 1594 1975 1078
Size: 214 302 381 3131
Fragments arranged by size:
3131 381 302 214
BsrBI CCG'CTC
Cuts at: 50 917 2320 2374 3858 4023 50
Size: 867 1403 54 1484 165 55
Fragments arranged by size:
1484 1403 867 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1940 1940
Size: 4028
BsrFI r'CCGG_y
Cuts at: 2026 2207 2416 2026
Size: 181 209 3638
Fragments arranged by size:
3638 209 181
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 1405 1405
Size: 4028
BstBI TT'CG_AA
Cuts at: 2389 2389
Size: 4028
BstYI r'GATC_y
Cuts at: 1 1053 1520 1543 1878 2124 3136 3222 3234
Size: 1052 467 23 335 246 1012 86 12
Cuts at: 3234 3580 1
Size: 346 449
Fragments arranged by size:
1052 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 299 320 476 572 107
Size: 192 21 156 96 3563
Fragments arranged by size:
3563 192 156 96 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 589 1978 2159 2322 589
Size: 1389 181 163 2295
Fragments arranged by size:
2295 1389 181 163
BtsI GCAGTG_nn'
Cuts at: 1271 1271
Size: 4028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 504 539 504
Size: 35 3993
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 165 1734 2592 165
Size: 1569 858 1601
Fragments arranged by size:
1601 1569 858
EaeI y'GGCC_r
Cuts at: 180 302 687 696 1613 1787 2178 2205 3550
Size: 122 385 9 917 174 391 27 1345
Cuts at: 3550 180
Size: 658
Fragments arranged by size:
1345 917 658 391 385 174 122 27
9
EagI C'GGCC_G
Cuts at: 687 696 1613 687
Size: 9 917 3102
Fragments arranged by size:
3102 917 9
EarI CTCTTCn'nnn_
Cuts at: 907 2051 2261 907
Size: 1144 210 2674
Fragments arranged by size:
2674 1144 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 304 800 2236 2546 2692 3838 304
Size: 496 1436 310 146 1146 494
Fragments arranged by size:
1436 1146 496 494 310 146
EcoICRI GAG'CTC
Cuts at: 1040 1040
Size: 4028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1853 2285 3032 3300 364
Size: 1489 432 747 268 1092
Fragments arranged by size:
1489 1092 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 736 977 736
Size: 241 3787
EcoO109I rG'GnC_Cy
Cuts at: 1167 1167
Size: 4028
EcoRI G'AATT_C
Cuts at: 114 1032 114
Size: 918 3110
EcoRV GAT'ATC
Cuts at: 642 642
Size: 4028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 423 2047 423
Size: 1624 2404
FspI TGC'GCA
Cuts at: 1809 1809
Size: 4028
HaeII r_GCGC'y
Cuts at: 246 1462 1710 2732 3711 246
Size: 1216 248 1022 979 563
Fragments arranged by size:
1216 1022 979 563 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 493 525 631 663 1870 1900 1902 1932 493
Size: 32 106 32 1207 30 2 30 2589
Fragments arranged by size:
2589 1207 106 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 393 2699 2883 393
Size: 2306 184 1538
Fragments arranged by size:
2306 1538 184
MscI TGG'CCA
Cuts at: 1789 1789
Size: 4028
MslI CAynn'nnrTG
Cuts at: 2017 2144 2017
Size: 127 3901
MspA1I CmG'CkG
Cuts at: 574 1139 1453 1813 2826 3071 574
Size: 565 314 360 1013 245 1531
Fragments arranged by size:
1531 1013 565 360 314 245
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NcoI C'CATG_G
Cuts at: 107 299 107
Size: 192 3836
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NspI r_CATG'y
Cuts at: 1081 1581 2488 1081
Size: 500 907 2621
Fragments arranged by size:
2621 907 500
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PflMI CCAn_nnn'nTGG
Cuts at: 321 609 321
Size: 288 3740
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3996
PspOMI G'GGCC_C
Cuts at: 904 904
Size: 4028
PstI C_TGCA'G
Cuts at: 1075 1760 1075
Size: 685 3343
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 1453 1813 1453
Size: 360 3668
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 1042 1042
Size: 4028
SacII CC_GC'GG
Cuts at: 575 575
Size: 4028
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 907 2051 2261 907
Size: 1144 210 2674
Fragments arranged by size:
2674 1144 210
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SfcI C'TryA_G
Cuts at: 33 879 1071 1756 2749 2940 33
Size: 846 192 685 993 191 1121
Fragments arranged by size:
1121 993 846 685 192 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 179 695 179
Size: 516 3512
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 361 682 1050 361
Size: 321 368 3339
Fragments arranged by size:
3339 368 321
SmlI C'TyrA_G
Cuts at: 1183 2098 2590 2852 1183
Size: 915 492 262 2359
Fragments arranged by size:
2359 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
StuI AGG'CCT
Cuts at: 193 419 193
Size: 226 3802
StyI C'CwwG_G
Cuts at: 107 299 392 632 737 1162 1516 107
Size: 192 93 240 105 425 354 2619
Fragments arranged by size:
2619 425 354 240 192 105 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1611 1744 2203 2412 1611
Size: 133 459 209 3227
Fragments arranged by size:
3227 459 209 133
TatI w'GTAC_w
Cuts at: 3543 3543
Size: 4028
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 3996
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1523 1523
Size: 4028
XmaI C'CCGG_G
Cuts at: 359 680 1048 359
Size: 321 368 3339
Fragments arranged by size:
3339 368 321
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AscI AvrII BaeI
BbsI BfrBI BmtI BpmI BsaXI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI