Restriction map for
pF1KE0719
(Circular) (Six-base) MAPSORT of: pF1KE0719.seq Check: 4306 from: 1 to: 4028
pF1KE0719 4028 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1066 1066
Size: 4028
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 301 388 511 1853 2285 3032 3300 223
Size: 78 87 123 1342 432 747 268 951
Fragments arranged by size:
1342 951 747 432 268 123 87 78
AflIII A'CryG_T
Cuts at: 1380 2484 3461 1380
Size: 1104 977 1947
Fragments arranged by size:
1947 1104 977
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AleI CACnn'nnGTG
Cuts at: 655 655
Size: 4028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 907 907
Size: 4028
AlwNI CAG_nnn'CTG
Cuts at: 366 441 504 1333 2900 366
Size: 75 63 829 1567 1494
Fragments arranged by size:
1567 1494 829 75 63
ApaLI G'TGCA_C
Cuts at: 316 2798 316
Size: 2482 1546
ApoI r'AATT_y
Cuts at: 1032 3381 3637 1032
Size: 2349 256 1423
Fragments arranged by size:
2349 1423 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 619 790 1048 619
Size: 171 258 3599
Fragments arranged by size:
3599 258 171
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 592 625 592
Size: 33 3995
BamHI G'GATC_C
Cuts at: 1053 1053
Size: 4028
BanI G'GyrC_C
Cuts at: 145 523 567 1044 1458 1706 1741 145
Size: 378 44 477 414 248 35 2432
Fragments arranged by size:
2432 477 414 378 248 44 35
BanII G_rGCy'C
Cuts at: 404 980 1042 2072 404
Size: 576 62 1030 2360
Fragments arranged by size:
2360 1030 576 62
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BbsI GAAGACnn'nnnn_
Cuts at: 785 785
Size: 4028
BbvCI CC'TCA_GC
Cuts at: 355 355
Size: 4028
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4028
BglII A'GATC_T
Cuts at: 260 1543 3580 260
Size: 1283 2037 708
Fragments arranged by size:
2037 1283 708
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 148 320 572 866 1653 1746 2802 148
Size: 172 252 294 787 93 1056 1374
Fragments arranged by size:
1374 1056 787 294 252 172 93
BmgBI CAC'GTC
Cuts at: 312 312
Size: 4028
BmrI ACTGGGnnnn_n'
Cuts at: 1240 1321 1656 3800 1240
Size: 81 335 2144 1468
Fragments arranged by size:
2144 1468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 892 687
Size: 205 3823
Bpu10I CC'TnA_GC
Cuts at: 355 974 355
Size: 619 3409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 1204 2083 2575 2873 256
Size: 948 879 492 298 1411
Fragments arranged by size:
1411 948 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 479 3732 479
Size: 3253 775
BsaAI yAC'GTr
Cuts at: 1381 1381
Size: 4028
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 1459 1707 2409 3905 1459
Size: 248 702 1496 1582
Fragments arranged by size:
1582 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1217 1738 2416 2690 2837 3248 1217
Size: 521 678 274 147 411 1997
Fragments arranged by size:
1997 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 367 397 660 690 367
Size: 30 263 30 3705
Fragments arranged by size:
3705 263 30 30
BseYI C'CCAG_C
Cuts at: 762 772 800 919 984 1453 2211 2788 3284
Size: 10 28 119 65 469 758 577 496
Cuts at: 3284 762
Size: 1506
Fragments arranged by size:
1506 758 577 496 469 119 65 28
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 394
Size: 4028
BsiEI CG_ry'CG
Cuts at: 104 1570 1616 2824 104
Size: 1466 46 1208 1308
Fragments arranged by size:
1466 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 320 404 1042 1820 2010 2802 320
Size: 84 638 778 190 792 1546
Fragments arranged by size:
1546 792 778 638 190 84
Bsp1286I G_dGCh'C
Cuts at: 148 320 404 572 866 980 1042 1653 1746
Size: 172 84 168 294 114 62 611 93
Cuts at: 1746 1820 2010 2072 2802 148
Size: 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 294 190 172 168 114
93 84 74 62 62
BspEI T'CCGG_A
Cuts at: 3248 3248
Size: 4028
BspHI T'CATG_A
Cuts at: 3204 3204
Size: 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 512 899 1078 1292 1594 1975 512
Size: 387 179 214 302 381 2565
Fragments arranged by size:
2565 387 381 302 214 179
BsrBI CCG'CTC
Cuts at: 50 2320 2374 3858 4023 50
Size: 2270 54 1484 165 55
Fragments arranged by size:
2270 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1940 1940
Size: 4028
BsrFI r'CCGG_y
Cuts at: 2026 2207 2416 2026
Size: 181 209 3638
Fragments arranged by size:
3638 209 181
BsrGI T'GTAC_A
Cuts at: 161 161
Size: 4028
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 677 906 1405 677
Size: 229 499 3300
Fragments arranged by size:
3300 499 229
BstBI TT'CG_AA
Cuts at: 2389 2389
Size: 4028
BstEII G'GTnAC_C
Cuts at: 795 795
Size: 4028
BstXI CCAn_nnnn'nTGG
Cuts at: 534 657 534
Size: 123 3905
BstYI r'GATC_y
Cuts at: 1 260 1053 1520 1543 1878 2124 3136 3222
Size: 259 793 467 23 335 246 1012 86
Cuts at: 3222 3234 3580 1
Size: 12 346 449
Fragments arranged by size:
1012 793 467 449 346 335 259 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4028
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 2159 2322 1978
Size: 181 163 3684
Fragments arranged by size:
3684 181 163
BtsI GCAGTG_nn'
Cuts at: 245 1271 245
Size: 1026 3002
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 1734 2592 1734
Size: 858 3170
EaeI y'GGCC_r
Cuts at: 1613 1787 2178 2205 3550 1613
Size: 174 391 27 1345 2091
Fragments arranged by size:
2091 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1613 1613
Size: 4028
EarI CTCTTCn'nnn_
Cuts at: 436 848 2051 2261 436
Size: 412 1203 210 2203
Fragments arranged by size:
2203 1203 412 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1006 2236 2546 2692 3838 1006
Size: 1230 310 146 1146 1196
Fragments arranged by size:
1230 1196 1146 310 146
EcoICRI GAG'CTC
Cuts at: 402 1040 402
Size: 638 3390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 301 388 511 687 892 1853 2285 3032
Size: 78 87 123 176 205 961 432 747
Cuts at: 3032 3300 223
Size: 268 951
Fragments arranged by size:
961 951 747 432 268 205 176 123
87 78
EcoNI CCTnn'n_nnAGG
Cuts at: 358 358
Size: 4028
EcoO109I rG'GnC_Cy
Cuts at: 237 363 888 1167 237
Size: 126 525 279 3098
Fragments arranged by size:
3098 525 279 126
EcoRI G'AATT_C
Cuts at: 1032 1032
Size: 4028
EcoRV GAT'ATC
Cuts at: 663 663
Size: 4028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 840 2047 840
Size: 1207 2821
FspI TGC'GCA
Cuts at: 577 1809 577
Size: 1232 2796
HaeII r_GCGC'y
Cuts at: 1462 1710 2732 3711 1462
Size: 248 1022 979 1779
Fragments arranged by size:
1779 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 684 716 1870 1900 1902 1932 684
Size: 32 1154 30 2 30 2780
Fragments arranged by size:
2780 1154 32 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 324 2699 2883 324
Size: 2375 184 1469
Fragments arranged by size:
2375 1469 184
MscI TGG'CCA
Cuts at: 1789 1789
Size: 4028
MslI CAynn'nnrTG
Cuts at: 112 655 2017 2144 112
Size: 543 1362 127 1996
Fragments arranged by size:
1996 1362 543 127
MspA1I CmG'CkG
Cuts at: 192 438 655 804 927 988 1139 1453 1813
Size: 246 217 149 123 61 151 314 360
Cuts at: 1813 2826 3071 192
Size: 1013 245 1149
Fragments arranged by size:
1149 1013 360 314 246 245 217 151
149 123 61
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NcoI C'CATG_G
Cuts at: 107 107
Size: 4028
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NspI r_CATG'y
Cuts at: 1081 1581 2488 1081
Size: 500 907 2621
Fragments arranged by size:
2621 907 500
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PflMI CCAn_nnn'nTGG
Cuts at: 594 594
Size: 4028
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3996
PpuMI rG'GwC_Cy
Cuts at: 237 363 888 237
Size: 126 525 3377
Fragments arranged by size:
3377 525 126
PstI C_TGCA'G
Cuts at: 446 911 1010 1075 1760 446
Size: 465 99 65 685 2714
Fragments arranged by size:
2714 685 465 99 65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 192 438 655 804 988 1453 1813 192
Size: 246 217 149 184 465 360 2407
Fragments arranged by size:
2407 465 360 246 217 184 149
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 404 1042 404
Size: 638 3390
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SanDI GG'GwC_CC
Cuts at: 237 237
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 2051 2261 2051
Size: 210 3818
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SfcI C'TryA_G
Cuts at: 33 442 907 1006 1071 1756 2749 2940 33
Size: 409 465 99 65 685 993 191 1121
Fragments arranged by size:
1121 993 685 465 409 191 99 65
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 792 1050 792
Size: 258 3770
SmlI C'TyrA_G
Cuts at: 271 1183 2098 2590 2852 271
Size: 912 915 492 262 1447
Fragments arranged by size:
1447 915 912 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
StyI C'CwwG_G
Cuts at: 107 1162 1516 107
Size: 1055 354 2619
Fragments arranged by size:
2619 1055 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 511 601 786 814 1611 1744 2203 2412 511
Size: 90 185 28 797 133 459 209 2127
Fragments arranged by size:
2127 797 459 209 185 133 90 28
TatI w'GTAC_w
Cuts at: 161 374 3543 161
Size: 213 3169 646
Fragments arranged by size:
3169 646 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 3996
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 846 945 1523 846
Size: 99 578 3351
Fragments arranged by size:
3351 578 99
XmaI C'CCGG_G
Cuts at: 790 1048 790
Size: 258 3770
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BcgI BfrBI BglI BmtI BseRI BsiWI BsmI
BsmBI BssHII BstZ17I Bsu36I ClaI CspCI DraIII FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI