Restriction map for
pF1KE0722
(Circular) (Six-base) MAPSORT of: pF1KE0722.seq Check: 1008 from: 1 to: 4358
pF1KE0722 4358 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 286 600 286
Size: 314 4044
AccI GT'mk_AC
Cuts at: 674 1136 1396 674
Size: 462 260 3636
Fragments arranged by size:
3636 462 260
Acc65I G'GTAC_C
Cuts at: 1374 1374
Size: 4358
AclI AA'CG_TT
Cuts at: 4198 4198
Size: 4358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2615 3362 3630 2183
Size: 432 747 268 2911
Fragments arranged by size:
2911 747 432 268
AflIII A'CryG_T
Cuts at: 1710 2814 3791 1710
Size: 1104 977 2277
Fragments arranged by size:
2277 1104 977
AgeI A'CCGG_T
Cuts at: 2746 2746
Size: 4358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 704 704
Size: 4358
AlwNI CAG_nnn'CTG
Cuts at: 1084 1663 3230 1084
Size: 579 1567 2212
Fragments arranged by size:
2212 1567 579
ApaI G_GGCC'C
Cuts at: 246 246
Size: 4358
ApaLI G'TGCA_C
Cuts at: 802 3128 802
Size: 2326 2032
ApoI r'AATT_y
Cuts at: 1362 3711 3967 1362
Size: 2349 256 1753
Fragments arranged by size:
2349 1753 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4358
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
AvaI C'yCGr_G
Cuts at: 418 1117 1378 418
Size: 699 261 3398
Fragments arranged by size:
3398 699 261
BamHI G'GATC_C
Cuts at: 1383 1383
Size: 4358
BanI G'GyrC_C
Cuts at: 948 1374 1788 2036 2071 948
Size: 426 414 248 35 3235
Fragments arranged by size:
3235 426 414 248 35
BanII G_rGCy'C
Cuts at: 246 848 938 1372 2402 246
Size: 602 90 434 1030 2202
Fragments arranged by size:
2202 1030 602 434 90
BbeI G_GCGC'C
Cuts at: 1792 2040 1792
Size: 248 4110
BbsI GAAGACnn'nnnn_
Cuts at: 535 535
Size: 4358
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4324
BciVI GTATCCnnnnn_n'
Cuts at: 2256 3017 4358 2256
Size: 761 1341 2256
Fragments arranged by size:
2256 1341 761
BclI T'GATC_A
Cuts at: 1878 1878
Size: 4358
BglI GCCn_nnn'nGGC
Cuts at: 661 661
Size: 4358
BglII A'GATC_T
Cuts at: 1873 3910 1873
Size: 2037 2321
BlpI GC'TnA_GC
Cuts at: 1470 1470
Size: 4358
Bme1580I G_kGCm'C
Cuts at: 246 806 931 953 1983 2076 3132 246
Size: 560 125 22 1030 93 1056 1472
Fragments arranged by size:
1472 1056 1030 560 125 93 22
BmrI ACTGGGnnnn_n'
Cuts at: 1570 1651 1986 4130 1570
Size: 81 335 2144 1798
Fragments arranged by size:
2144 1798 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 333 2725 333
Size: 2392 1966
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1282 1363 718
Size: 564 81 3713
Fragments arranged by size:
3713 564 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1534 2413 2905 3203 1534
Size: 879 492 298 2689
Fragments arranged by size:
2689 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1093 4062 1093
Size: 2969 1389
BsaAI yAC'GTr
Cuts at: 1711 1711
Size: 4358
BsaBI GATnn'nnATC
Cuts at: 6 1872 3909 6
Size: 1866 2037 455
Fragments arranged by size:
2037 1866 455
BsaHI Gr'CG_yC
Cuts at: 890 1789 2037 2739 4235 890
Size: 899 248 702 1496 1013
Fragments arranged by size:
1496 1013 899 702 248
BsaWI w'CCGG_w
Cuts at: 731 945 1547 2068 2746 3020 3167 3578 731
Size: 214 602 521 678 274 147 411 1511
Fragments arranged by size:
1511 678 602 521 411 274 214 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 244 274 802 832 244
Size: 30 528 30 3770
Fragments arranged by size:
3770 528 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 268 418 436 238
Size: 30 150 18 4160
Fragments arranged by size:
4160 150 30 18
BseYI C'CCAG_C
Cuts at: 113 1783 2541 3118 3614 113
Size: 1670 758 577 496 857
Fragments arranged by size:
1670 857 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 298 1033 1174 162
Size: 136 735 141 3346
Fragments arranged by size:
3346 735 141 136
BsiEI CG_ry'CG
Cuts at: 104 972 1900 1946 3154 104
Size: 868 928 46 1208 1308
Fragments arranged by size:
1308 1208 928 868 46
BsiHKAI G_wGCw'C
Cuts at: 731 806 848 925 1372 2150 2340 3132 731
Size: 75 42 77 447 778 190 792 1957
Fragments arranged by size:
1957 792 778 447 190 77 75 42
Bsp1286I G_dGCh'C
Cuts at: 246 731 806 848 925 931 938 953 1372
Size: 485 75 42 77 6 7 15 419
Cuts at: 1372 1983 2076 2150 2340 2402 3132 246
Size: 611 93 74 190 62 730 1472
Fragments arranged by size:
1472 730 611 485 419 190 93 77
75 74 62 42 15 7 6
BspEI T'CCGG_A
Cuts at: 3578 3578
Size: 4358
BspHI T'CATG_A
Cuts at: 3534 3534
Size: 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 286 600 1408 1622 1924 2305 286
Size: 314 808 214 302 381 2339
Fragments arranged by size:
2339 808 381 314 302 214
BsrBI CCG'CTC
Cuts at: 50 258 669 943 1122 2650 2704 4188 4353
Size: 208 411 274 179 1528 54 1484 165
Cuts at: 4353 50
Size: 55
Fragments arranged by size:
1528 1484 411 274 208 179 165 55
54
BsrDI GCAATG_nn'
Cuts at: 686 2270 686
Size: 1584 2774
BsrFI r'CCGG_y
Cuts at: 770 2356 2537 2746 770
Size: 1586 181 209 2382
Fragments arranged by size:
2382 1586 209 181
BssSI C'ACGA_G
Cuts at: 2629 2987 2629
Size: 358 4000
BstAPI GCAn_nnn'nTGC
Cuts at: 1084 1735 1084
Size: 651 3707
BstBI TT'CG_AA
Cuts at: 2719 2719
Size: 4358
BstEII G'GTnAC_C
Cuts at: 978 978
Size: 4358
BstXI CCAn_nnnn'nTGG
Cuts at: 1074 1074
Size: 4358
BstYI r'GATC_y
Cuts at: 1 1383 1850 1873 2208 2454 3466 3552 3564
Size: 1382 467 23 335 246 1012 86 12
Cuts at: 3564 3910 1
Size: 346 449
Fragments arranged by size:
1382 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 675 675
Size: 4358
Bsu36I CC'TnA_GG
Cuts at: 322 322
Size: 4358
BtgI C'CryG_G
Cuts at: 515 1247 515
Size: 732 3626
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1131 2308 2489 2652 1131
Size: 1177 181 163 2837
Fragments arranged by size:
2837 1177 181 163
BtsI GCAGTG_nn'
Cuts at: 646 832 1157 1601 646
Size: 186 325 444 3403
Fragments arranged by size:
3403 444 325 186
DraI TTT'AAA
Cuts at: 1357 1357
Size: 4358
DrdI GACnn_nn'nnGTC
Cuts at: 2064 2922 2064
Size: 858 3500
EaeI y'GGCC_r
Cuts at: 300 597 653 1943 2117 2508 2535 3880 300
Size: 297 56 1290 174 391 27 1345 778
Fragments arranged by size:
1345 1290 778 391 297 174 56 27
EagI C'GGCC_G
Cuts at: 1943 1943
Size: 4358
EarI CTCTTCn'nnn_
Cuts at: 481 613 2381 2591 481
Size: 132 1768 210 2248
Fragments arranged by size:
2248 1768 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 265 2566 2876 3022 4168 265
Size: 2301 310 146 1146 455
Fragments arranged by size:
2301 1146 455 310 146
EcoICRI GAG'CTC
Cuts at: 846 1370 846
Size: 524 3834
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1282 1363 2183 2615 3362 3630 718
Size: 564 81 820 432 747 268 1446
Fragments arranged by size:
1446 820 747 564 432 268 81
EcoNI CCTnn'n_nnAGG
Cuts at: 400 400
Size: 4358
EcoO109I rG'GnC_Cy
Cuts at: 242 326 1497 242
Size: 84 1171 3103
Fragments arranged by size:
3103 1171 84
EcoRI G'AATT_C
Cuts at: 1362 1362
Size: 4358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4358
FspI TGC'GCA
Cuts at: 692 2139 692
Size: 1447 2911
HaeII r_GCGC'y
Cuts at: 593 1204 1792 2040 3062 4041 593
Size: 611 588 248 1022 979 910
Fragments arranged by size:
1022 979 910 611 588 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2200 2230 2232 2262 2200
Size: 30 2 30 4296
Fragments arranged by size:
4296 30 30 2
HincII GTy'rAC
Cuts at: 1397 1397
Size: 4358
HindIII A'AGCT_T
Cuts at: 1348 1348
Size: 4358
KasI G'GCGC_C
Cuts at: 1788 2036 1788
Size: 248 4110
KpnI G_GTAC'C
Cuts at: 1378 1378
Size: 4358
MluI A'CGCG_T
Cuts at: 3791 3791
Size: 4358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3029 3213 3029
Size: 184 4174
MscI TGG'CCA
Cuts at: 302 2119 302
Size: 1817 2541
MslI CAynn'nnrTG
Cuts at: 1150 2347 2474 1150
Size: 1197 127 3034
Fragments arranged by size:
3034 1197 127
MspA1I CmG'CkG
Cuts at: 117 186 384 696 756 987 1249 1469 1783
Size: 69 198 312 60 231 262 220 314
Cuts at: 1783 2143 3156 3401 117
Size: 360 1013 245 1074
Fragments arranged by size:
1074 1013 360 314 312 262 245 231
220 198 69 60
NaeI GCC'GGC
Cuts at: 2539 2539
Size: 4358
NarI GG'CG_CC
Cuts at: 1789 2037 1789
Size: 248 4110
NcoI C'CATG_G
Cuts at: 515 515
Size: 4358
NgoMIV G'CCGG_C
Cuts at: 2537 2537
Size: 4358
NspI r_CATG'y
Cuts at: 1411 1911 2818 1411
Size: 500 907 2951
Fragments arranged by size:
2951 907 500
PciI A'CATG_T
Cuts at: 2814 2814
Size: 4358
PfoI T'CCnGG_A
Cuts at: 1259 1271 1259
Size: 12 4346
PmeI GTTT'AAAC
Cuts at: 1357 1357
Size: 4358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 4326
PspOMI G'GGCC_C
Cuts at: 242 242
Size: 4358
PstI C_TGCA'G
Cuts at: 122 185 883 1405 2090 122
Size: 63 698 522 685 2390
Fragments arranged by size:
2390 698 685 522 63
PvuI CG_AT'CG
Cuts at: 104 972 104
Size: 868 3490
PvuII CAG'CTG
Cuts at: 117 384 696 756 1783 2143 117
Size: 267 312 60 1027 360 2332
Fragments arranged by size:
2332 1027 360 312 267 60
RsrII CG'GwC_CG
Cuts at: 2553 2553
Size: 4358
SacI G_AGCT'C
Cuts at: 848 1372 848
Size: 524 3834
SacII CC_GC'GG
Cuts at: 1250 1250
Size: 4358
SalI G'TCGA_C
Cuts at: 1395 1395
Size: 4358
SapI GCTCTTCn'nnn_
Cuts at: 481 2381 2591 481
Size: 1900 210 2248
Fragments arranged by size:
2248 1900 210
SbfI CC_TGCA'GG
Cuts at: 1405 1405
Size: 4358
ScaI AGT'ACT
Cuts at: 3875 3875
Size: 4358
SfcI C'TryA_G
Cuts at: 33 118 181 879 1401 2086 3079 3270 33
Size: 85 63 698 522 685 993 191 1121
Fragments arranged by size:
1121 993 698 685 522 191 85 63
SfoI GGC'GCC
Cuts at: 1790 2038 1790
Size: 248 4110
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
SmaI CCC'GGG
Cuts at: 1380 1380
Size: 4358
SmlI C'TyrA_G
Cuts at: 418 1513 2428 2920 3182 418
Size: 1095 915 492 262 1594
Fragments arranged by size:
1594 1095 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4358
SphI G_CATG'C
Cuts at: 1411 1911 1411
Size: 500 3858
StuI AGG'CCT
Cuts at: 574 748 574
Size: 174 4184
StyI C'CwwG_G
Cuts at: 515 1492 1846 515
Size: 977 354 3027
Fragments arranged by size:
3027 977 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 789 1941 2074 2533 2742 789
Size: 1152 133 459 209 2405
Fragments arranged by size:
2405 1152 459 209 133
TatI w'GTAC_w
Cuts at: 3873 3873
Size: 4358
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 282 314 726 758 1116 1148 1276 1308 3875
Size: 32 412 32 358 32 128 32 2567
Cuts at: 3875 3907 282
Size: 32 733
Fragments arranged by size:
2567 733 412 358 128 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 478 2155 478
Size: 1677 2681
XbaI T'CTAG_A
Cuts at: 1389 1389
Size: 4358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1853 1853
Size: 4358
XhoI C'TCGA_G
Cuts at: 418 418
Size: 4358
XmaI C'CCGG_G
Cuts at: 1378 1378
Size: 4358
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BssHII ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI