Restriction map for
pF1KE0723
(Circular) (Six-base) MAPSORT of: pF1KE0723.seq Check: 8966 from: 1 to: 4031
pF1KE0723 4031 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1069 1069
Size: 4031
Acc65I G'GTAC_C
Cuts at: 1047 1047
Size: 4031
AclI AA'CG_TT
Cuts at: 3871 3871
Size: 4031
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 1856 2288 3035 3303 106
Size: 1750 432 747 268 834
Fragments arranged by size:
1750 834 747 432 268
AflIII A'CryG_T
Cuts at: 1383 2487 3464 1383
Size: 1104 977 1950
Fragments arranged by size:
1950 1104 977
AgeI A'CCGG_T
Cuts at: 2419 2419
Size: 4031
AhdI GACnn_n'nnGTC
Cuts at: 156 553 156
Size: 397 3634
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 682 758 682
Size: 76 3955
AlwNI CAG_nnn'CTG
Cuts at: 360 424 1336 2903 360
Size: 64 912 1567 1488
Fragments arranged by size:
1567 1488 912 64
ApaLI G'TGCA_C
Cuts at: 265 2801 265
Size: 2536 1495
ApoI r'AATT_y
Cuts at: 1035 3384 3640 1035
Size: 2349 256 1426
Fragments arranged by size:
2349 1426 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4031
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
AvaI C'yCGr_G
Cuts at: 170 311 551 932 1051 170
Size: 141 240 381 119 3150
Fragments arranged by size:
3150 381 240 141 119
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 575 608 575
Size: 33 3998
BamHI G'GATC_C
Cuts at: 1056 1056
Size: 4031
BanI G'GyrC_C
Cuts at: 793 1047 1461 1709 1744 793
Size: 254 414 248 35 3080
Fragments arranged by size:
3080 414 254 248 35
BanII G_rGCy'C
Cuts at: 959 1045 2075 959
Size: 86 1030 2915
Fragments arranged by size:
2915 1030 86
BbeI G_GCGC'C
Cuts at: 797 1465 1713 797
Size: 668 248 3115
Fragments arranged by size:
3115 668 248
BbsI GAAGACnn'nnnn_
Cuts at: 168 168
Size: 4031
BciVI GTATCCnnnnn_n'
Cuts at: 1929 2690 4031 1929
Size: 761 1341 1929
Fragments arranged by size:
1929 1341 761
BclI T'GATC_A
Cuts at: 1551 1551
Size: 4031
BglII A'GATC_T
Cuts at: 1546 3583 1546
Size: 2037 1994
BlpI GC'TnA_GC
Cuts at: 636 721 1143 636
Size: 85 422 3524
Fragments arranged by size:
3524 422 85
Bme1580I G_kGCm'C
Cuts at: 269 527 1656 1749 2805 269
Size: 258 1129 93 1056 1495
Fragments arranged by size:
1495 1129 1056 258 93
BmrI ACTGGGnnnn_n'
Cuts at: 1243 1324 1659 3803 1243
Size: 81 335 2144 1471
Fragments arranged by size:
2144 1471 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 398
Size: 4031
Bpu10I CC'TnA_GC
Cuts at: 386 386
Size: 4031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 2086 2578 2876 1207
Size: 879 492 298 2362
Fragments arranged by size:
2362 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 217 3735 217
Size: 3518 513
BsaAI yAC'GTr
Cuts at: 1384 1384
Size: 4031
BsaBI GATnn'nnATC
Cuts at: 6 1545 3582 6
Size: 1539 2037 455
Fragments arranged by size:
2037 1539 455
BsaHI Gr'CG_yC
Cuts at: 794 1462 1710 2412 3908 794
Size: 668 248 702 1496 917
Fragments arranged by size:
1496 917 702 668 248
BsaWI w'CCGG_w
Cuts at: 1220 1741 2419 2693 2840 3251 1220
Size: 521 678 274 147 411 2000
Fragments arranged by size:
2000 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 324 1456 2214 2791 3287 324
Size: 1132 758 577 496 1068
Fragments arranged by size:
1132 1068 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1573 1619 2827 104
Size: 1469 46 1208 1308
Fragments arranged by size:
1469 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 269 1045 1823 2013 2805 269
Size: 776 778 190 792 1495
Fragments arranged by size:
1495 792 778 776 190
BsmBI CGTCTCn'nnnn_
Cuts at: 929 929
Size: 4031
Bsp1286I G_dGCh'C
Cuts at: 269 527 959 1045 1656 1749 1823 2013 2075
Size: 258 432 86 611 93 74 190 62
Cuts at: 2075 2805 269
Size: 730 1495
Fragments arranged by size:
1495 730 611 432 258 190 93 86
74 62
BspEI T'CCGG_A
Cuts at: 3251 3251
Size: 4031
BspHI T'CATG_A
Cuts at: 3207 3207
Size: 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1014 1081 1295 1597 1978 1014
Size: 67 214 302 381 3067
Fragments arranged by size:
3067 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 614 2323 2377 3861 4026 50
Size: 564 1709 54 1484 165 55
Fragments arranged by size:
1709 1484 564 165 55 54
BsrDI GCAATG_nn'
Cuts at: 138 1943 138
Size: 1805 2226
BsrFI r'CCGG_y
Cuts at: 253 796 866 2029 2210 2419 253
Size: 543 70 1163 181 209 1865
Fragments arranged by size:
1865 1163 543 209 181 70
BssSI C'ACGA_G
Cuts at: 2302 2660 2302
Size: 358 3673
BstAPI GCAn_nnn'nTGC
Cuts at: 424 1408 424
Size: 984 3047
BstBI TT'CG_AA
Cuts at: 2392 2392
Size: 4031
BstYI r'GATC_y
Cuts at: 1 1056 1523 1546 1881 2127 3139 3225 3237
Size: 1055 467 23 335 246 1012 86 12
Cuts at: 3237 3583 1
Size: 346 449
Fragments arranged by size:
1055 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 959 1981 2162 2325 959
Size: 1022 181 163 2665
Fragments arranged by size:
2665 1022 181 163
BtsI GCAGTG_nn'
Cuts at: 666 843 1274 666
Size: 177 431 3423
Fragments arranged by size:
3423 431 177
ClaI AT'CG_AT
Cuts at: 710 710
Size: 4031
DraI TTT'AAA
Cuts at: 1030 1030
Size: 4031
DrdI GACnn_nn'nnGTC
Cuts at: 1737 2595 1737
Size: 858 3173
EaeI y'GGCC_r
Cuts at: 243 251 347 868 1616 1790 2181 2208 3553
Size: 8 96 521 748 174 391 27 1345
Cuts at: 3553 243
Size: 721
Fragments arranged by size:
1345 748 721 521 391 174 96 27
8
EagI C'GGCC_G
Cuts at: 1616 1616
Size: 4031
EarI CTCTTCn'nnn_
Cuts at: 493 971 2054 2264 493
Size: 478 1083 210 2260
Fragments arranged by size:
2260 1083 478 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2239 2549 2695 3841 2239
Size: 310 146 1146 2429
Fragments arranged by size:
2429 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1043 1043
Size: 4031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 398 1856 2288 3035 3303 106
Size: 292 1458 432 747 268 834
Fragments arranged by size:
1458 834 747 432 292 268
EcoNI CCTnn'n_nnAGG
Cuts at: 913 913
Size: 4031
EcoO109I rG'GnC_Cy
Cuts at: 1016 1170 1016
Size: 154 3877
EcoRI G'AATT_C
Cuts at: 1035 1035
Size: 4031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2050 2050
Size: 4031
FspI TGC'GCA
Cuts at: 1812 1812
Size: 4031
HaeII r_GCGC'y
Cuts at: 306 797 1465 1713 2735 3714 306
Size: 491 668 248 1022 979 623
Fragments arranged by size:
1022 979 668 623 491 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 142 174 539 571 1873 1903 1905 1935 142
Size: 32 365 32 1302 30 2 30 2238
Fragments arranged by size:
2238 1302 365 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1070 1070
Size: 4031
HindIII A'AGCT_T
Cuts at: 118 118
Size: 4031
KasI G'GCGC_C
Cuts at: 793 1461 1709 793
Size: 668 248 3115
Fragments arranged by size:
3115 668 248
KpnI G_GTAC'C
Cuts at: 1051 1051
Size: 4031
MluI A'CGCG_T
Cuts at: 3464 3464
Size: 4031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2702 2886 2702
Size: 184 3847
MscI TGG'CCA
Cuts at: 1792 1792
Size: 4031
MslI CAynn'nnrTG
Cuts at: 370 2020 2147 370
Size: 1650 127 2254
Fragments arranged by size:
2254 1650 127
MspA1I CmG'CkG
Cuts at: 319 622 824 1142 1456 1816 2829 3074 319
Size: 303 202 318 314 360 1013 245 1276
Fragments arranged by size:
1276 1013 360 318 314 303 245 202
NaeI GCC'GGC
Cuts at: 255 798 868 2212 255
Size: 543 70 1344 2074
Fragments arranged by size:
2074 1344 543 70
NarI GG'CG_CC
Cuts at: 794 1462 1710 794
Size: 668 248 3115
Fragments arranged by size:
3115 668 248
NgoMIV G'CCGG_C
Cuts at: 253 796 866 2210 253
Size: 543 70 1344 2074
Fragments arranged by size:
2074 1344 543 70
NspI r_CATG'y
Cuts at: 1084 1584 2491 1084
Size: 500 907 2624
Fragments arranged by size:
2624 907 500
PasI CC'CwG_GG
Cuts at: 527 915 527
Size: 388 3643
PciI A'CATG_T
Cuts at: 2487 2487
Size: 4031
PmeI GTTT'AAAC
Cuts at: 1030 1030
Size: 4031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3999
PstI C_TGCA'G
Cuts at: 671 1078 1763 671
Size: 407 685 2939
Fragments arranged by size:
2939 685 407
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4031
PvuII CAG'CTG
Cuts at: 319 622 1456 1816 319
Size: 303 834 360 2534
Fragments arranged by size:
2534 834 360 303
RsrII CG'GwC_CG
Cuts at: 2226 2226
Size: 4031
SacI G_AGCT'C
Cuts at: 1045 1045
Size: 4031
SalI G'TCGA_C
Cuts at: 1068 1068
Size: 4031
SapI GCTCTTCn'nnn_
Cuts at: 493 971 2054 2264 493
Size: 478 1083 210 2260
Fragments arranged by size:
2260 1083 478 210
SbfI CC_TGCA'GG
Cuts at: 1078 1078
Size: 4031
ScaI AGT'ACT
Cuts at: 292 3548 292
Size: 3256 775
SfcI C'TryA_G
Cuts at: 33 667 1074 1759 2752 2943 33
Size: 634 407 685 993 191 1121
Fragments arranged by size:
1121 993 685 634 407 191
SfoI GGC'GCC
Cuts at: 795 1463 1711 795
Size: 668 248 3115
Fragments arranged by size:
3115 668 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
SgrAI Cr'CCGG_yG
Cuts at: 796 796
Size: 4031
SmaI CCC'GGG
Cuts at: 313 553 1053 313
Size: 240 500 3291
Fragments arranged by size:
3291 500 240
SmlI C'TyrA_G
Cuts at: 170 1186 2101 2593 2855 170
Size: 1016 915 492 262 1346
Fragments arranged by size:
1346 1016 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4031
SphI G_CATG'C
Cuts at: 1084 1584 1084
Size: 500 3531
SrfI GCCC'GGGC
Cuts at: 313 313
Size: 4031
StuI AGG'CCT
Cuts at: 908 908
Size: 4031
StyI C'CwwG_G
Cuts at: 401 1165 1519 401
Size: 764 354 2913
Fragments arranged by size:
2913 764 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1614 1747 2206 2415 1614
Size: 133 459 209 3230
Fragments arranged by size:
3230 459 209 133
TatI w'GTAC_w
Cuts at: 290 3546 290
Size: 3256 775
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3548 3580 3548
Size: 32 3999
Tth111I GACn'n_nGTC
Cuts at: 1828 1828
Size: 4031
XbaI T'CTAG_A
Cuts at: 1062 1062
Size: 4031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1526 1526
Size: 4031
XhoI C'TCGA_G
Cuts at: 170 170
Size: 4031
XmaI C'CCGG_G
Cuts at: 311 551 1051 311
Size: 240 500 3291
Fragments arranged by size:
3291 500 240
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SnaBI SspI
SwaI XmnI ZraI