Restriction map for
pF1KE0728
(Circular) (Six-base) MAPSORT of: pF1KE0728.seq Check: 7863 from: 1 to: 4367
pF1KE0728 4367 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 989 989
Size: 4367
AccI GT'mk_AC
Cuts at: 821 1405 821
Size: 584 3783
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 999 2192 2624 3371 3639 528
Size: 471 1193 432 747 268 1256
Fragments arranged by size:
1256 1193 747 471 432 268
AfeI AGC'GCT
Cuts at: 411 411
Size: 4367
AflIII A'CryG_T
Cuts at: 747 1719 2823 3800 747
Size: 972 1104 977 1314
Fragments arranged by size:
1314 1104 977 972
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1108 1108
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 214 1672 3239 214
Size: 1458 1567 1342
Fragments arranged by size:
1567 1458 1342
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 832 1371 3720 3976 832
Size: 539 2349 256 1223
Fragments arranged by size:
2349 1223 539 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 343 1387 343
Size: 1044 3323
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1332 1365 1332
Size: 33 4334
BamHI G'GATC_C
Cuts at: 555 1392 555
Size: 837 3530
BanI G'GyrC_C
Cuts at: 427 700 1026 1234 1383 1797 2045 2080 427
Size: 273 326 208 149 414 248 35 2714
Fragments arranged by size:
2714 414 326 273 248 208 149 35
BanII G_rGCy'C
Cuts at: 397 1381 2411 397
Size: 984 1030 2353
Fragments arranged by size:
2353 1030 984
BbeI G_GCGC'C
Cuts at: 1030 1801 2049 1030
Size: 771 248 3348
Fragments arranged by size:
3348 771 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 646 680 820 854 90
Size: 34 522 34 140 34 3603
Fragments arranged by size:
3603 522 140 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 286 950 1333 2265 3026 4367 286
Size: 664 383 932 761 1341 286
Fragments arranged by size:
1341 932 761 664 383 286
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BglI GCCn_nnn'nGGC
Cuts at: 112 519 112
Size: 407 3960
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 233 1479 233
Size: 1246 3121
Bme1580I G_kGCm'C
Cuts at: 897 1992 2085 3141 897
Size: 1095 93 1056 2123
Fragments arranged by size:
2123 1095 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 246 893 1579 1660 1995 4139 246
Size: 647 686 81 335 2144 474
Fragments arranged by size:
2144 686 647 474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 645 2734 645
Size: 2089 2278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1196 1196
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 104 1543 2422 2914 3212 104
Size: 1439 879 492 298 1259
Fragments arranged by size:
1439 1259 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1303 4071 1303
Size: 2768 1599
BsaAI yAC'GTr
Cuts at: 748 1720 748
Size: 972 3395
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 115 1027 1798 2046 2748 4244 115
Size: 912 771 248 702 1496 238
Fragments arranged by size:
1496 912 771 702 248 238
BsaWI w'CCGG_w
Cuts at: 558 1556 2077 2755 3029 3176 3587 558
Size: 998 521 678 274 147 411 1338
Fragments arranged by size:
1338 998 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1127 1792 2550 3127 3623 1127
Size: 665 758 577 496 1871
Fragments arranged by size:
1871 758 665 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 836 836
Size: 4367
BsiEI CG_ry'CG
Cuts at: 104 962 996 1909 1955 3163 104
Size: 858 34 913 46 1208 1308
Fragments arranged by size:
1308 1208 913 858 46 34
BsiHKAI G_wGCw'C
Cuts at: 350 1381 2159 2349 3141 350
Size: 1031 778 190 792 1576
Fragments arranged by size:
1576 1031 792 778 190
BsmI GAATG_Cn'
Cuts at: 1248 1248
Size: 4367
BsmBI CGTCTCn'nnnn_
Cuts at: 122 426 122
Size: 304 4063
Bsp1286I G_dGCh'C
Cuts at: 350 397 897 1381 1992 2085 2159 2349 2411
Size: 47 500 484 611 93 74 190 62
Cuts at: 2411 3141 350
Size: 730 1576
Fragments arranged by size:
1576 730 611 500 484 190 93 74
62 47
BspEI T'CCGG_A
Cuts at: 558 3587 558
Size: 3029 1338
BspHI T'CATG_A
Cuts at: 3543 3543
Size: 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 989 1417 1631 1933 2314 989
Size: 428 214 302 381 3042
Fragments arranged by size:
3042 428 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2659 2713 4197 4362 50
Size: 2609 54 1484 165 55
Fragments arranged by size:
2609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 929 1312 2279 929
Size: 383 967 3017
Fragments arranged by size:
3017 967 383
BsrFI r'CCGG_y
Cuts at: 162 2365 2546 2755 162
Size: 2203 181 209 1774
Fragments arranged by size:
2203 1774 209 181
BsrGI T'GTAC_A
Cuts at: 887 1004 887
Size: 117 4250
BssSI C'ACGA_G
Cuts at: 405 2638 2996 405
Size: 2233 358 1776
Fragments arranged by size:
2233 1776 358
BstAPI GCAn_nnn'nTGC
Cuts at: 214 1744 214
Size: 1530 2837
BstBI TT'CG_AA
Cuts at: 656 2728 656
Size: 2072 2295
BstYI r'GATC_y
Cuts at: 1 555 877 1392 1859 1882 2217 2463 3475
Size: 554 322 515 467 23 335 246 1012
Cuts at: 3475 3561 3573 3919 1
Size: 86 12 346 449
Fragments arranged by size:
1012 554 515 467 449 346 335 322
246 86 23 12
BtgI C'CryG_G
Cuts at: 281 335 704 956 990 1022 281
Size: 54 369 252 34 32 3626
Fragments arranged by size:
3626 369 252 54 34 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 1142 1610 1142
Size: 468 3899
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 465 959 1952 2126 2517 2544 3889 465
Size: 494 993 174 391 27 1345 943
Fragments arranged by size:
1345 993 943 494 391 174 27
EagI C'GGCC_G
Cuts at: 959 1952 959
Size: 993 3374
EarI CTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 415 1070 2575 2885 3031 4177 415
Size: 655 1505 310 146 1146 605
Fragments arranged by size:
1505 1146 655 605 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 999 1196 2192 2624 3371 3639 528
Size: 471 197 996 432 747 268 1256
Fragments arranged by size:
1256 996 747 471 432 268 197
EcoO109I rG'GnC_Cy
Cuts at: 1506 1506
Size: 4367
EcoRI G'AATT_C
Cuts at: 832 1371 832
Size: 539 3828
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4367
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 413 1030 1801 2049 3071 4050 413
Size: 617 771 248 1022 979 730
Fragments arranged by size:
1022 979 771 730 617 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2209 2239 2241 2271 2209
Size: 30 2 30 4305
Fragments arranged by size:
4305 30 30 2
HincII GTy'rAC
Cuts at: 774 1406 774
Size: 632 3735
KasI G'GCGC_C
Cuts at: 1026 1797 2045 1026
Size: 771 248 3348
Fragments arranged by size:
3348 771 248
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3038 3222 3038
Size: 184 4183
MscI TGG'CCA
Cuts at: 467 2128 467
Size: 1661 2706
MslI CAynn'nnrTG
Cuts at: 140 1286 2356 2483 140
Size: 1146 1070 127 2024
Fragments arranged by size:
2024 1146 1070 127
MspA1I CmG'CkG
Cuts at: 214 283 958 992 1024 1150 1478 1792 2152
Size: 69 675 34 32 126 328 314 360
Cuts at: 2152 3165 3410 214
Size: 1013 245 1171
Fragments arranged by size:
1171 1013 675 360 328 314 245 126
69 34 32
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 1027 1798 2046 1027
Size: 771 248 3348
Fragments arranged by size:
3348 771 248
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NotI GC'GGCC_GC
Cuts at: 959 959
Size: 4367
NspI r_CATG'y
Cuts at: 529 1420 1920 2827 529
Size: 891 500 907 2069
Fragments arranged by size:
2069 907 891 500
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PfoI T'CCnGG_A
Cuts at: 180 551 180
Size: 371 3996
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PmlI CAC'GTG
Cuts at: 748 748
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PstI C_TGCA'G
Cuts at: 1100 1414 2099 1100
Size: 314 685 3368
Fragments arranged by size:
3368 685 314
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 1792 2152 1792
Size: 360 4007
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SacII CC_GC'GG
Cuts at: 284 959 993 1025 284
Size: 675 34 32 3626
Fragments arranged by size:
3626 675 34 32
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 822 1096 1155 1410 2095 3088 3279 33
Size: 789 274 59 255 685 993 191 1121
Fragments arranged by size:
1121 993 789 685 274 255 191 59
SfoI GGC'GCC
Cuts at: 1028 1799 2047 1028
Size: 771 248 3348
Fragments arranged by size:
3348 771 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 119 1522 2437 2929 3191 119
Size: 1403 915 492 262 1295
Fragments arranged by size:
1403 1295 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StyI C'CwwG_G
Cuts at: 1501 1855 1501
Size: 354 4013
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 802 1950 2083 2542 2751 802
Size: 1148 133 459 209 2418
Fragments arranged by size:
2418 1148 459 209 133
TatI w'GTAC_w
Cuts at: 320 887 1004 3882 320
Size: 567 117 2878 805
Fragments arranged by size:
2878 805 567 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4335
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NotI NspI PciI PfoI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BseRI
BsiWI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NruI NsiI PacI PasI PflMI
PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI