Restriction map for
pF1KE0736
(Circular) (Six-base) MAPSORT of: pF1KE0736.seq Check: 3629 from: 1 to: 4382
pF1KE0736 4382 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1420 1420
Size: 4382
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 1257 2207 2639 3386 3654 464
Size: 793 950 432 747 268 1192
Fragments arranged by size:
1192 950 793 747 432 268
AflIII A'CryG_T
Cuts at: 1340 1734 2838 3815 1340
Size: 394 1104 977 1907
Fragments arranged by size:
1907 1104 977 394
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1319 1319
Size: 4382
AlwNI CAG_nnn'CTG
Cuts at: 1687 3254 1687
Size: 1567 2815
ApaI G_GGCC'C
Cuts at: 654 654
Size: 4382
ApaLI G'TGCA_C
Cuts at: 1294 3152 1294
Size: 1858 2524
ApoI r'AATT_y
Cuts at: 1386 3735 3991 1386
Size: 2349 256 1777
Fragments arranged by size:
2349 1777 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 662 1402 662
Size: 740 3642
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 1398 1812 2060 2095 1398
Size: 414 248 35 3685
Fragments arranged by size:
3685 414 248 35
BanII G_rGCy'C
Cuts at: 293 489 527 565 654 881 1019 1396 2426
Size: 196 38 38 89 227 138 377 1030
Cuts at: 2426 293
Size: 2249
Fragments arranged by size:
2249 1030 377 227 196 138 89 38
38
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BbsI GAAGACnn'nnnn_
Cuts at: 431 431
Size: 4382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4348
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 1902 1902
Size: 4382
BglII A'GATC_T
Cuts at: 1897 3934 1897
Size: 2037 2345
BlpI GC'TnA_GC
Cuts at: 468 1494 468
Size: 1026 3356
Bme1580I G_kGCm'C
Cuts at: 654 1298 2007 2100 3156 654
Size: 644 709 93 1056 1880
Fragments arranged by size:
1880 1056 709 644 93
BmrI ACTGGGnnnn_n'
Cuts at: 897 1594 1675 2010 4154 897
Size: 697 81 335 2144 1125
Fragments arranged by size:
2144 1125 697 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 312 2749 312
Size: 2437 1945
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 907 181
Size: 726 3656
Bpu10I CC'TnA_GC
Cuts at: 875 1006 875
Size: 131 4251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1174 1558 2437 2929 3227 1174
Size: 384 879 492 298 2329
Fragments arranged by size:
2329 879 492 384 298
BsaI GGTCTCn'nnnn_
Cuts at: 185 1153 4086 185
Size: 968 2933 481
Fragments arranged by size:
2933 968 481
BsaAI yAC'GTr
Cuts at: 1735 1735
Size: 4382
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 1103 1813 2061 2763 4259 1103
Size: 710 248 702 1496 1226
Fragments arranged by size:
1496 1226 710 702 248
BsaWI w'CCGG_w
Cuts at: 151 853 915 1367 1571 2092 2770 3044 3191
Size: 702 62 452 204 521 678 274 147
Cuts at: 3191 3602 151
Size: 411 931
Fragments arranged by size:
931 702 678 521 452 411 274 204
147 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 113 143 243 273 113
Size: 30 100 30 4222
Fragments arranged by size:
4222 100 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 147 634 718 961 147
Size: 487 84 243 3568
Fragments arranged by size:
3568 487 243 84
BseYI C'CCAG_C
Cuts at: 230 880 1018 1251 1807 2565 3142 3638 230
Size: 650 138 233 556 758 577 496 974
Fragments arranged by size:
974 758 650 577 556 496 233 138
BsiEI CG_ry'CG
Cuts at: 104 1924 1970 3178 104
Size: 1820 46 1208 1308
Fragments arranged by size:
1820 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1298 1396 2174 2364 3156 1298
Size: 98 778 190 792 2524
Fragments arranged by size:
2524 792 778 190 98
BsmBI CGTCTCn'nnnn_
Cuts at: 263 263
Size: 4382
Bsp1286I G_dGCh'C
Cuts at: 293 489 527 565 654 881 1019 1298 1396
Size: 196 38 38 89 227 138 279 98
Cuts at: 1396 2007 2100 2174 2364 2426 3156 293
Size: 611 93 74 190 62 730 1519
Fragments arranged by size:
1519 730 611 279 227 196 190 138
98 93 89 74 62 38 38
BspEI T'CCGG_A
Cuts at: 1367 3602 1367
Size: 2235 2147
BspHI T'CATG_A
Cuts at: 3558 3558
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1121 1432 1646 1948 2329 1121
Size: 311 214 302 381 3174
Fragments arranged by size:
3174 381 311 302 214
BsrBI CCG'CTC
Cuts at: 50 738 2674 2728 4212 4377 50
Size: 688 1936 54 1484 165 55
Fragments arranged by size:
1936 1484 688 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2294 2294
Size: 4382
BsrFI r'CCGG_y
Cuts at: 2380 2561 2770 2380
Size: 181 209 3992
Fragments arranged by size:
3992 209 181
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 1143 1143
Size: 4382
BstYI r'GATC_y
Cuts at: 1 554 1044 1407 1874 1897 2232 2478 3490
Size: 553 490 363 467 23 335 246 1012
Cuts at: 3490 3576 3588 3934 1
Size: 86 12 346 449
Fragments arranged by size:
1012 553 490 467 449 363 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 701 701
Size: 4382
BtgI C'CryG_G
Cuts at: 314 314
Size: 4382
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2332 2513 2676 2332
Size: 181 163 4038
Fragments arranged by size:
4038 181 163
BtsI GCAGTG_nn'
Cuts at: 273 1051 1625 273
Size: 778 574 3030
Fragments arranged by size:
3030 778 574
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 769 2088 2946 769
Size: 1319 858 2205
Fragments arranged by size:
2205 1319 858
EaeI y'GGCC_r
Cuts at: 548 939 1361 1967 2141 2532 2559 3904 548
Size: 391 422 606 174 391 27 1345 1026
Fragments arranged by size:
1345 1026 606 422 391 391 174 27
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 916 2405 2615 916
Size: 1489 210 2683
Fragments arranged by size:
2683 1489 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 216 369 2590 2900 3046 4192 216
Size: 153 2221 310 146 1146 406
Fragments arranged by size:
2221 1146 406 310 153 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 464 907 1257 2207 2639 3386 3654 181
Size: 283 443 350 950 432 747 268 909
Fragments arranged by size:
950 909 747 443 432 350 283 268
EcoNI CCTnn'n_nnAGG
Cuts at: 679 679
Size: 4382
EcoO109I rG'GnC_Cy
Cuts at: 124 498 650 651 695 1521 124
Size: 374 152 1 44 826 2985
Fragments arranged by size:
2985 826 374 152 44 1
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1191 2401 1191
Size: 1210 3172
FspI TGC'GCA
Cuts at: 373 1150 2163 373
Size: 777 1013 2592
Fragments arranged by size:
2592 1013 777
HaeII r_GCGC'y
Cuts at: 140 902 1816 2064 3086 4065 140
Size: 762 914 248 1022 979 457
Fragments arranged by size:
1022 979 914 762 457 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 564 596 2224 2254 2256 2286 564
Size: 32 1628 30 2 30 2660
Fragments arranged by size:
2660 1628 32 30 30 2
HincII GTy'rAC
Cuts at: 762 1421 762
Size: 659 3723
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3053 3237 3053
Size: 184 4198
MscI TGG'CCA
Cuts at: 1363 2143 1363
Size: 780 3602
MslI CAynn'nnrTG
Cuts at: 1213 1244 1301 2371 2498 1213
Size: 31 57 1070 127 3097
Fragments arranged by size:
3097 1070 127 57 31
MspA1I CmG'CkG
Cuts at: 810 1493 1807 2167 3180 3425 810
Size: 683 314 360 1013 245 1767
Fragments arranged by size:
1767 1013 683 360 314 245
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 4382
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 4382
NspI r_CATG'y
Cuts at: 1344 1435 1935 2842 1344
Size: 91 500 907 2884
Fragments arranged by size:
2884 907 500 91
PasI CC'CwG_GG
Cuts at: 719 1029 719
Size: 310 4072
PciI A'CATG_T
Cuts at: 1340 2838 1340
Size: 1498 2884
PfoI T'CCnGG_A
Cuts at: 241 241
Size: 4382
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 4350
PpuMI rG'GwC_Cy
Cuts at: 124 498 124
Size: 374 4008
PspOMI G'GGCC_C
Cuts at: 650 650
Size: 4382
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 207 239 207
Size: 32 4350
PstI C_TGCA'G
Cuts at: 981 1429 2114 981
Size: 448 685 3249
Fragments arranged by size:
3249 685 448
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 810 1807 2167 810
Size: 997 360 3025
Fragments arranged by size:
3025 997 360
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 916 2405 2615 916
Size: 1489 210 2683
Fragments arranged by size:
2683 1489 210
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SexAI A'CCwGG_T
Cuts at: 1067 1142 1067
Size: 75 4307
SfcI C'TryA_G
Cuts at: 33 144 977 1425 2110 3103 3294 33
Size: 111 833 448 685 993 191 1121
Fragments arranged by size:
1121 993 833 685 448 191 111
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 664 1404 664
Size: 740 3642
SmlI C'TyrA_G
Cuts at: 1189 1537 2452 2944 3206 1189
Size: 348 915 492 262 2365
Fragments arranged by size:
2365 915 492 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
StyI C'CwwG_G
Cuts at: 209 566 787 1232 1516 1870 209
Size: 357 221 445 284 354 2721
Fragments arranged by size:
2721 445 357 354 284 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1965 2098 2557 2766 1965
Size: 133 459 209 3581
Fragments arranged by size:
3581 459 209 133
TatI w'GTAC_w
Cuts at: 358 3897 358
Size: 3539 843
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 113 145 243 275 3899 3931 113
Size: 32 98 32 3624 32 564
Fragments arranged by size:
3624 564 98 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1026 1877 1026
Size: 851 3531
XmaI C'CCGG_G
Cuts at: 662 1402 662
Size: 740 3642
XmnI GAAnn'nnTTC
Cuts at: 1174 1174
Size: 4382
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI