Restriction map for
pF1KE0741
(Circular) (Six-base) MAPSORT of: pF1KE0741.seq Check: 1701 from: 1 to: 4058
pF1KE0741 4058 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1096 1096
Size: 4058
Acc65I G'GTAC_C
Cuts at: 1074 1074
Size: 4058
AclI AA'CG_TT
Cuts at: 3898 3898
Size: 4058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 1883 2315 3062 3330 136
Size: 1747 432 747 268 864
Fragments arranged by size:
1747 864 747 432 268
AflIII A'CryG_T
Cuts at: 1410 2514 3491 1410
Size: 1104 977 1977
Fragments arranged by size:
1977 1104 977
AgeI A'CCGG_T
Cuts at: 2446 2446
Size: 4058
AleI CACnn'nnGTG
Cuts at: 260 260
Size: 4058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 278 278
Size: 4058
AlwNI CAG_nnn'CTG
Cuts at: 1363 2930 1363
Size: 1567 2491
ApaLI G'TGCA_C
Cuts at: 2828 2828
Size: 4058
ApoI r'AATT_y
Cuts at: 884 949 1000 1062 3411 3667 884
Size: 65 51 62 2349 256 1275
Fragments arranged by size:
2349 1275 256 65 62 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 4058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
AvaI C'yCGr_G
Cuts at: 212 1078 212
Size: 866 3192
BamHI G'GATC_C
Cuts at: 1083 1083
Size: 4058
BanI G'GyrC_C
Cuts at: 1074 1488 1736 1771 1074
Size: 414 248 35 3361
Fragments arranged by size:
3361 414 248 35
BanII G_rGCy'C
Cuts at: 219 305 1072 2102 219
Size: 86 767 1030 2175
Fragments arranged by size:
2175 1030 767 86
BbeI G_GCGC'C
Cuts at: 1492 1740 1492
Size: 248 3810
BbsI GAAGACnn'nnnn_
Cuts at: 228 228
Size: 4058
BbvCI CC'TCA_GC
Cuts at: 724 724
Size: 4058
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4024
BciVI GTATCCnnnnn_n'
Cuts at: 1956 2717 4058 1956
Size: 761 1341 1956
Fragments arranged by size:
1956 1341 761
BclI T'GATC_A
Cuts at: 1578 1578
Size: 4058
BglII A'GATC_T
Cuts at: 347 1573 3610 347
Size: 1226 2037 795
Fragments arranged by size:
2037 1226 795
BlpI GC'TnA_GC
Cuts at: 1170 1170
Size: 4058
Bme1580I G_kGCm'C
Cuts at: 1683 1776 2832 1683
Size: 93 1056 2909
Fragments arranged by size:
2909 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1270 1351 1686 3830 1270
Size: 81 335 2144 1498
Fragments arranged by size:
2144 1498 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 303 2425 303
Size: 2122 1936
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 273
Size: 4058
Bpu10I CC'TnA_GC
Cuts at: 724 724
Size: 4058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1234 2113 2605 2903 889
Size: 345 879 492 298 2044
Fragments arranged by size:
2044 879 492 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 145 496 3762 145
Size: 351 3266 441
Fragments arranged by size:
3266 441 351
BsaAI yAC'GTr
Cuts at: 1411 1411
Size: 4058
BsaBI GATnn'nnATC
Cuts at: 6 1572 3609 6
Size: 1566 2037 455
Fragments arranged by size:
2037 1566 455
BsaHI Gr'CG_yC
Cuts at: 627 1489 1737 2439 3935 627
Size: 862 248 702 1496 750
Fragments arranged by size:
1496 862 750 702 248
BsaWI w'CCGG_w
Cuts at: 1247 1768 2446 2720 2867 3278 1247
Size: 521 678 274 147 411 2027
Fragments arranged by size:
2027 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1483 2241 2818 3314 1483
Size: 758 577 496 2227
Fragments arranged by size:
2227 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1600 1646 2854 104
Size: 1496 46 1208 1308
Fragments arranged by size:
1496 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 305 1072 1850 2040 2832 305
Size: 767 778 190 792 1531
Fragments arranged by size:
1531 792 778 767 190
Bsp1286I G_dGCh'C
Cuts at: 219 305 1072 1683 1776 1850 2040 2102 2832
Size: 86 767 611 93 74 190 62 730
Cuts at: 2832 219
Size: 1445
Fragments arranged by size:
1445 767 730 611 190 93 86 74
62
BspEI T'CCGG_A
Cuts at: 3278 3278
Size: 4058
BspHI T'CATG_A
Cuts at: 3234 3234
Size: 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1108 1322 1624 2005 1108
Size: 214 302 381 3161
Fragments arranged by size:
3161 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2350 2404 3888 4053 50
Size: 2300 54 1484 165 55
Fragments arranged by size:
2300 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1970 1970
Size: 4058
BsrFI r'CCGG_y
Cuts at: 2056 2237 2446 2056
Size: 181 209 3668
Fragments arranged by size:
3668 209 181
BsrGI T'GTAC_A
Cuts at: 875 875
Size: 4058
BssSI C'ACGA_G
Cuts at: 2329 2687 2329
Size: 358 3700
BstAPI GCAn_nnn'nTGC
Cuts at: 1435 1435
Size: 4058
BstBI TT'CG_AA
Cuts at: 2419 2419
Size: 4058
BstYI r'GATC_y
Cuts at: 1 347 1083 1550 1573 1908 2154 3166 3252
Size: 346 736 467 23 335 246 1012 86
Cuts at: 3252 3264 3610 1
Size: 12 346 449
Fragments arranged by size:
1012 736 467 449 346 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 266 809 266
Size: 543 3515
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2008 2189 2352 2008
Size: 181 163 3714
Fragments arranged by size:
3714 181 163
BtsI GCAGTG_nn'
Cuts at: 1301 1301
Size: 4058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 897 932 897
Size: 35 4023
DraI TTT'AAA
Cuts at: 365 1057 365
Size: 692 3366
DrdI GACnn_nn'nnGTC
Cuts at: 1003 1764 2622 1003
Size: 761 858 2439
Fragments arranged by size:
2439 858 761
EaeI y'GGCC_r
Cuts at: 1643 1817 2208 2235 3580 1643
Size: 174 391 27 1345 2121
Fragments arranged by size:
2121 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1643 1643
Size: 4058
EarI CTCTTCn'nnn_
Cuts at: 1038 2081 2291 1038
Size: 1043 210 2805
Fragments arranged by size:
2805 1043 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2266 2576 2722 3868 2266
Size: 310 146 1146 2456
Fragments arranged by size:
2456 1146 310 146
EcoICRI GAG'CTC
Cuts at: 303 1070 303
Size: 767 3291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 273 1883 2315 3062 3330 136
Size: 137 1610 432 747 268 864
Fragments arranged by size:
1610 864 747 432 268 137
EcoO109I rG'GnC_Cy
Cuts at: 799 1197 799
Size: 398 3660
EcoRI G'AATT_C
Cuts at: 884 1062 884
Size: 178 3880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 4058
FspI TGC'GCA
Cuts at: 1839 1839
Size: 4058
HaeII r_GCGC'y
Cuts at: 1492 1740 2762 3741 1492
Size: 248 1022 979 1809
Fragments arranged by size:
1809 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 712 744 1900 1930 1932 1962 712
Size: 32 1156 30 2 30 2808
Fragments arranged by size:
2808 1156 32 30 30 2
HincII GTy'rAC
Cuts at: 1097 1097
Size: 4058
KasI G'GCGC_C
Cuts at: 1488 1736 1488
Size: 248 3810
KpnI G_GTAC'C
Cuts at: 1078 1078
Size: 4058
MfeI C'AATT_G
Cuts at: 845 845
Size: 4058
MluI A'CGCG_T
Cuts at: 3491 3491
Size: 4058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 323 2729 2913 323
Size: 2406 184 1468
Fragments arranged by size:
2406 1468 184
MscI TGG'CCA
Cuts at: 1819 1819
Size: 4058
MslI CAynn'nnrTG
Cuts at: 260 635 910 2047 2174 260
Size: 375 275 1137 127 2144
Fragments arranged by size:
2144 1137 375 275 127
MspA1I CmG'CkG
Cuts at: 249 1169 1483 1843 2856 3101 249
Size: 920 314 360 1013 245 1206
Fragments arranged by size:
1206 1013 920 360 314 245
NaeI GCC'GGC
Cuts at: 2239 2239
Size: 4058
NarI GG'CG_CC
Cuts at: 1489 1737 1489
Size: 248 3810
NgoMIV G'CCGG_C
Cuts at: 2237 2237
Size: 4058
NspI r_CATG'y
Cuts at: 1111 1611 2518 1111
Size: 500 907 2651
Fragments arranged by size:
2651 907 500
PciI A'CATG_T
Cuts at: 2514 2514
Size: 4058
PfoI T'CCnGG_A
Cuts at: 296 296
Size: 4058
PmeI GTTT'AAAC
Cuts at: 1057 1057
Size: 4058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 4026
PpuMI rG'GwC_Cy
Cuts at: 799 799
Size: 4058
PsiI TTA'TAA
Cuts at: 529 529
Size: 4058
PstI C_TGCA'G
Cuts at: 1105 1790 1105
Size: 685 3373
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4058
PvuII CAG'CTG
Cuts at: 249 1483 1843 249
Size: 1234 360 2464
Fragments arranged by size:
2464 1234 360
RsrII CG'GwC_CG
Cuts at: 2253 2253
Size: 4058
SacI G_AGCT'C
Cuts at: 305 1072 305
Size: 767 3291
SalI G'TCGA_C
Cuts at: 1095 1095
Size: 4058
SapI GCTCTTCn'nnn_
Cuts at: 2081 2291 2081
Size: 210 3848
SbfI CC_TGCA'GG
Cuts at: 1105 1105
Size: 4058
ScaI AGT'ACT
Cuts at: 3575 3575
Size: 4058
SfcI C'TryA_G
Cuts at: 33 1101 1786 2779 2970 33
Size: 1068 685 993 191 1121
Fragments arranged by size:
1121 1068 993 685 191
SfoI GGC'GCC
Cuts at: 1490 1738 1490
Size: 248 3810
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
SmaI CCC'GGG
Cuts at: 214 1080 214
Size: 866 3192
SmlI C'TyrA_G
Cuts at: 868 1213 2128 2620 2882 868
Size: 345 915 492 262 2044
Fragments arranged by size:
2044 915 492 345 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4058
SphI G_CATG'C
Cuts at: 1111 1611 1111
Size: 500 3558
SspI AAT'ATT
Cuts at: 615 615
Size: 4058
StyI C'CwwG_G
Cuts at: 146 1192 1546 146
Size: 1046 354 2658
Fragments arranged by size:
2658 1046 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 611 1641 1774 2233 2442 611
Size: 1030 133 459 209 2227
Fragments arranged by size:
2227 1030 459 209 133
TatI w'GTAC_w
Cuts at: 875 3573 875
Size: 2698 1360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3575 3607 3575
Size: 32 4026
Tth111I GACn'n_nGTC
Cuts at: 1855 1855
Size: 4058
XbaI T'CTAG_A
Cuts at: 1089 1089
Size: 4058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 273 1553 273
Size: 1280 2778
XmaI C'CCGG_G
Cuts at: 212 1078 212
Size: 866 3192
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI BseRI
BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI