Restriction map for
pF1KE0749
(Circular) (Six-base) MAPSORT of: pF1KE0749.seq Check: 8771 from: 1 to: 4091
pF1KE0749 4091 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 555 555
Size: 4091
AccI GT'mk_AC
Cuts at: 269 707 1129 269
Size: 438 422 3231
Fragments arranged by size:
3231 438 422
Acc65I G'GTAC_C
Cuts at: 1107 1107
Size: 4091
AclI AA'CG_TT
Cuts at: 3931 3931
Size: 4091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1916 2348 3095 3363 1916
Size: 432 747 268 2644
Fragments arranged by size:
2644 747 432 268
AflIII A'CryG_T
Cuts at: 1443 2547 3524 1443
Size: 1104 977 2010
Fragments arranged by size:
2010 1104 977
AgeI A'CCGG_T
Cuts at: 2479 2479
Size: 4091
AleI CACnn'nnGTG
Cuts at: 730 730
Size: 4091
AlwNI CAG_nnn'CTG
Cuts at: 570 912 1396 2963 570
Size: 342 484 1567 1698
Fragments arranged by size:
1698 1567 484 342
ApaI G_GGCC'C
Cuts at: 152 152
Size: 4091
ApaLI G'TGCA_C
Cuts at: 607 1032 2861 607
Size: 425 1829 1837
Fragments arranged by size:
1837 1829 425
ApoI r'AATT_y
Cuts at: 1095 3444 3700 1095
Size: 2349 256 1486
Fragments arranged by size:
2349 1486 256
AscI GG'CGCG_CC
Cuts at: 547 547
Size: 4091
AseI AT'TA_AT
Cuts at: 20 20
Size: 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
AvaI C'yCGr_G
Cuts at: 484 728 1111 484
Size: 244 383 3464
Fragments arranged by size:
3464 383 244
BamHI G'GATC_C
Cuts at: 752 1116 752
Size: 364 3727
BanI G'GyrC_C
Cuts at: 526 724 923 1107 1521 1769 1804 526
Size: 198 199 184 414 248 35 2813
Fragments arranged by size:
2813 414 248 199 198 184 35
BanII G_rGCy'C
Cuts at: 152 299 662 1105 2135 152
Size: 147 363 443 1030 2108
Fragments arranged by size:
2108 1030 443 363 147
BbeI G_GCGC'C
Cuts at: 530 1525 1773 530
Size: 995 248 2848
Fragments arranged by size:
2848 995 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4057
BciVI GTATCCnnnnn_n'
Cuts at: 1989 2750 4091 1989
Size: 761 1341 1989
Fragments arranged by size:
1989 1341 761
BclI T'GATC_A
Cuts at: 1611 1611
Size: 4091
BglI GCCn_nnn'nGGC
Cuts at: 356 356
Size: 4091
BglII A'GATC_T
Cuts at: 1606 3643 1606
Size: 2037 2054
BlpI GC'TnA_GC
Cuts at: 1203 1203
Size: 4091
Bme1580I G_kGCm'C
Cuts at: 152 371 611 1036 1716 1809 2865 152
Size: 219 240 425 680 93 1056 1378
Fragments arranged by size:
1378 1056 680 425 240 219 93
BmgBI CAC'GTC
Cuts at: 1053 1067 1053
Size: 14 4077
BmrI ACTGGGnnnn_n'
Cuts at: 1303 1384 1719 3863 1303
Size: 81 335 2144 1531
Fragments arranged by size:
2144 1531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 911 2458 911
Size: 1547 2544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 496 881 394
Size: 102 385 3604
Fragments arranged by size:
3604 385 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 679 1267 2146 2638 2936 646
Size: 33 588 879 492 298 1801
Fragments arranged by size:
1801 879 588 492 298 33
BsaI GGTCTCn'nnnn_
Cuts at: 897 3795 897
Size: 2898 1193
BsaAI yAC'GTr
Cuts at: 318 1444 318
Size: 1126 2965
BsaBI GATnn'nnATC
Cuts at: 6 1605 3642 6
Size: 1599 2037 455
Fragments arranged by size:
2037 1599 455
BsaHI Gr'CG_yC
Cuts at: 422 527 1004 1522 1770 2472 3968 422
Size: 105 477 518 248 702 1496 545
Fragments arranged by size:
1496 702 545 518 477 248 105
BsaWI w'CCGG_w
Cuts at: 652 881 917 1280 1801 2479 2753 2900 3311
Size: 229 36 363 521 678 274 147 411
Cuts at: 3311 652
Size: 1432
Fragments arranged by size:
1432 678 521 411 363 274 229 147
36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 634 664 634
Size: 30 4061
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 868 900 868
Size: 32 4059
BseYI C'CCAG_C
Cuts at: 243 798 987 1516 2274 2851 3347 243
Size: 555 189 529 758 577 496 987
Fragments arranged by size:
987 758 577 555 529 496 189
BsiEI CG_ry'CG
Cuts at: 104 365 952 1633 1679 2887 104
Size: 261 587 681 46 1208 1308
Fragments arranged by size:
1308 1208 681 587 261 46
BsiHKAI G_wGCw'C
Cuts at: 611 662 1036 1105 1883 2073 2865 611
Size: 51 374 69 778 190 792 1837
Fragments arranged by size:
1837 792 778 374 190 69 51
Bsp1286I G_dGCh'C
Cuts at: 152 299 371 611 662 1036 1105 1716 1809
Size: 147 72 240 51 374 69 611 93
Cuts at: 1809 1883 2073 2135 2865 152
Size: 74 190 62 730 1378
Fragments arranged by size:
1378 730 611 374 240 190 147 93
74 72 69 62 51
BspEI T'CCGG_A
Cuts at: 652 881 3311 652
Size: 229 2430 1432
Fragments arranged by size:
2430 1432 229
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 555 653 1031 1141 1355 1657 2038 555
Size: 98 378 110 214 302 381 2608
Fragments arranged by size:
2608 381 378 302 214 110 98
BsrBI CCG'CTC
Cuts at: 50 330 450 480 948 2383 2437 3921 4086
Size: 280 120 30 468 1435 54 1484 165
Cuts at: 4086 50
Size: 55
Fragments arranged by size:
1484 1435 468 280 165 120 55 54
30
BsrDI GCAATG_nn'
Cuts at: 2003 2003
Size: 4091
BsrFI r'CCGG_y
Cuts at: 489 2089 2270 2479 489
Size: 1600 181 209 2101
Fragments arranged by size:
2101 1600 209 181
BsrGI T'GTAC_A
Cuts at: 734 734
Size: 4091
BssHII G'CGCG_C
Cuts at: 413 415 547 413
Size: 2 132 3957
Fragments arranged by size:
3957 132 2
BssSI C'ACGA_G
Cuts at: 2362 2720 2362
Size: 358 3733
BstAPI GCAn_nnn'nTGC
Cuts at: 1468 1468
Size: 4091
BstBI TT'CG_AA
Cuts at: 2452 2452
Size: 4091
BstEII G'GTnAC_C
Cuts at: 333 333
Size: 4091
BstYI r'GATC_y
Cuts at: 1 179 752 857 1116 1583 1606 1941 2187
Size: 178 573 105 259 467 23 335 246
Cuts at: 2187 3199 3285 3297 3643 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 573 467 449 346 335 259 246
178 105 86 23 12
BtgI C'CryG_G
Cuts at: 771 771
Size: 4091
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 270 2041 2222 2385 270
Size: 1771 181 163 1976
Fragments arranged by size:
1976 1771 181 163
BtsI GCAGTG_nn'
Cuts at: 245 585 1334 245
Size: 340 749 3002
Fragments arranged by size:
3002 749 340
DraI TTT'AAA
Cuts at: 1090 1090
Size: 4091
DraIII CAC_nnn'GTG
Cuts at: 321 791 321
Size: 470 3621
DrdI GACnn_nn'nnGTC
Cuts at: 1003 1797 2655 1003
Size: 794 858 2439
Fragments arranged by size:
2439 858 794
EaeI y'GGCC_r
Cuts at: 572 782 949 1055 1676 1850 2241 2268 3613
Size: 210 167 106 621 174 391 27 1345
Cuts at: 3613 572
Size: 1050
Fragments arranged by size:
1345 1050 621 391 210 174 167 106
27
EagI C'GGCC_G
Cuts at: 949 1676 949
Size: 727 3364
EarI CTCTTCn'nnn_
Cuts at: 508 883 2114 2324 508
Size: 375 1231 210 2275
Fragments arranged by size:
2275 1231 375 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 214 418 2299 2609 2755 3901 214
Size: 204 1881 310 146 1146 404
Fragments arranged by size:
1881 1146 404 310 204 146
EcoICRI GAG'CTC
Cuts at: 660 1103 660
Size: 443 3648
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 496 881 1916 2348 3095 3363 394
Size: 102 385 1035 432 747 268 1122
Fragments arranged by size:
1122 1035 747 432 385 268 102
EcoO109I rG'GnC_Cy
Cuts at: 498 1230 498
Size: 732 3359
EcoRI G'AATT_C
Cuts at: 1095 1095
Size: 4091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 228 2110 228
Size: 1882 2209
FspI TGC'GCA
Cuts at: 391 1872 391
Size: 1481 2610
HaeII r_GCGC'y
Cuts at: 530 780 1525 1773 2795 3774 530
Size: 250 745 248 1022 979 847
Fragments arranged by size:
1022 979 847 745 250 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 634 666 937 969 1933 1963 1965 1995 634
Size: 32 271 32 964 30 2 30 2730
Fragments arranged by size:
2730 964 271 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1130 1130
Size: 4091
KasI G'GCGC_C
Cuts at: 526 1521 1769 526
Size: 995 248 2848
Fragments arranged by size:
2848 995 248
KpnI G_GTAC'C
Cuts at: 1111 1111
Size: 4091
MluI A'CGCG_T
Cuts at: 3524 3524
Size: 4091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2762 2946 2762
Size: 184 3907
MscI TGG'CCA
Cuts at: 1057 1852 1057
Size: 795 3296
MslI CAynn'nnrTG
Cuts at: 730 2080 2207 730
Size: 1350 127 2614
Fragments arranged by size:
2614 1350 127
MspA1I CmG'CkG
Cuts at: 543 596 912 1202 1516 1876 2889 3134 543
Size: 53 316 290 314 360 1013 245 1500
Fragments arranged by size:
1500 1013 360 316 314 290 245 53
NaeI GCC'GGC
Cuts at: 491 2272 491
Size: 1781 2310
NarI GG'CG_CC
Cuts at: 527 1522 1770 527
Size: 995 248 2848
Fragments arranged by size:
2848 995 248
NgoMIV G'CCGG_C
Cuts at: 489 2270 489
Size: 1781 2310
NspI r_CATG'y
Cuts at: 1144 1644 2551 1144
Size: 500 907 2684
Fragments arranged by size:
2684 907 500
PciI A'CATG_T
Cuts at: 2547 2547
Size: 4091
PflMI CCAn_nnn'nTGG
Cuts at: 791 791
Size: 4091
PfoI T'CCnGG_A
Cuts at: 1007 1007
Size: 4091
PmeI GTTT'AAAC
Cuts at: 1090 1090
Size: 4091
PmlI CAC'GTG
Cuts at: 318 318
Size: 4091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 4059
PpuMI rG'GwC_Cy
Cuts at: 498 498
Size: 4091
PspOMI G'GGCC_C
Cuts at: 148 148
Size: 4091
PstI C_TGCA'G
Cuts at: 188 1138 1823 188
Size: 950 685 2456
Fragments arranged by size:
2456 950 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4091
PvuII CAG'CTG
Cuts at: 596 912 1516 1876 596
Size: 316 604 360 2811
Fragments arranged by size:
2811 604 360 316
RsrII CG'GwC_CG
Cuts at: 2286 2286
Size: 4091
SacI G_AGCT'C
Cuts at: 662 1105 662
Size: 443 3648
SalI G'TCGA_C
Cuts at: 1128 1128
Size: 4091
SapI GCTCTTCn'nnn_
Cuts at: 883 2114 2324 883
Size: 1231 210 2650
Fragments arranged by size:
2650 1231 210
SbfI CC_TGCA'GG
Cuts at: 1138 1138
Size: 4091
ScaI AGT'ACT
Cuts at: 3608 3608
Size: 4091
SexAI A'CCwGG_T
Cuts at: 227 227
Size: 4091
SfcI C'TryA_G
Cuts at: 33 184 1134 1819 2812 3003 33
Size: 151 950 685 993 191 1121
Fragments arranged by size:
1121 993 950 685 191 151
SfoI GGC'GCC
Cuts at: 528 1523 1771 528
Size: 995 248 2848
Fragments arranged by size:
2848 995 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
SmaI CCC'GGG
Cuts at: 1113 1113
Size: 4091
SmlI C'TyrA_G
Cuts at: 661 694 1246 2161 2653 2915 661
Size: 33 552 915 492 262 1837
Fragments arranged by size:
1837 915 552 492 262 33
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4091
SphI G_CATG'C
Cuts at: 1144 1644 1144
Size: 500 3591
StyI C'CwwG_G
Cuts at: 194 1225 1579 194
Size: 1031 354 2706
Fragments arranged by size:
2706 1031 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 352 1674 1807 2266 2475 352
Size: 1322 133 459 209 1968
Fragments arranged by size:
1968 1322 459 209 133
TatI w'GTAC_w
Cuts at: 734 3606 734
Size: 2872 1219
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 4059
Tth111I GACn'n_nGTC
Cuts at: 703 1888 703
Size: 1185 2906
XbaI T'CTAG_A
Cuts at: 1122 1122
Size: 4091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1586 1586
Size: 4091
XmaI C'CCGG_G
Cuts at: 1111 1111
Size: 4091
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AvrII BaeI
BbsI BbvCI BfrBI BmtI Bpu10I BsgI BsiWI BsmI
BsmBI BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI