Restriction map for
pF1KE0751
(Circular) (Six-base) MAPSORT of: pF1KE0751.seq Check: 351 from: 1 to: 4097
pF1KE0751 4097 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1135 1135
Size: 4097
Acc65I G'GTAC_C
Cuts at: 1113 1113
Size: 4097
AclI AA'CG_TT
Cuts at: 3937 3937
Size: 4097
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1922 2354 3101 3369 1922
Size: 432 747 268 2650
Fragments arranged by size:
2650 747 432 268
AflIII A'CryG_T
Cuts at: 731 1449 2553 3530 731
Size: 718 1104 977 1298
Fragments arranged by size:
1298 1104 977 718
AgeI A'CCGG_T
Cuts at: 2485 2485
Size: 4097
AleI CACnn'nnGTG
Cuts at: 472 472
Size: 4097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 769 769
Size: 4097
AlwNI CAG_nnn'CTG
Cuts at: 949 1402 2969 949
Size: 453 1567 2077
Fragments arranged by size:
2077 1567 453
ApaI G_GGCC'C
Cuts at: 708 708
Size: 4097
ApaLI G'TGCA_C
Cuts at: 2867 2867
Size: 4097
ApoI r'AATT_y
Cuts at: 235 1101 3450 3706 235
Size: 866 2349 256 626
Fragments arranged by size:
2349 866 626 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4097
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
AvaI C'yCGr_G
Cuts at: 135 707 927 1029 1117 135
Size: 572 220 102 88 3115
Fragments arranged by size:
3115 572 220 102 88
BamHI G'GATC_C
Cuts at: 1122 1122
Size: 4097
BanI G'GyrC_C
Cuts at: 130 1113 1527 1775 1810 130
Size: 983 414 248 35 2417
Fragments arranged by size:
2417 983 414 248 35
BanII G_rGCy'C
Cuts at: 708 1030 1111 2141 708
Size: 322 81 1030 2664
Fragments arranged by size:
2664 1030 322 81
BbeI G_GCGC'C
Cuts at: 1531 1779 1531
Size: 248 3849
BciVI GTATCCnnnnn_n'
Cuts at: 1078 1995 2756 4097 1078
Size: 917 761 1341 1078
Fragments arranged by size:
1341 1078 917 761
BclI T'GATC_A
Cuts at: 1617 1617
Size: 4097
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4097
BglII A'GATC_T
Cuts at: 1612 3649 1612
Size: 2037 2060
BlpI GC'TnA_GC
Cuts at: 662 1209 662
Size: 547 3550
Bme1580I G_kGCm'C
Cuts at: 708 1722 1815 2871 708
Size: 1014 93 1056 1934
Fragments arranged by size:
1934 1056 1014 93
BmrI ACTGGGnnnn_n'
Cuts at: 1309 1390 1725 3869 1309
Size: 81 335 2144 1537
Fragments arranged by size:
2144 1537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 273
Size: 4097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 2152 2644 2942 1273
Size: 879 492 298 2428
Fragments arranged by size:
2428 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3801 3801
Size: 4097
BsaAI yAC'GTr
Cuts at: 1450 1450
Size: 4097
BsaBI GATnn'nnATC
Cuts at: 6 1611 3648 6
Size: 1605 2037 455
Fragments arranged by size:
2037 1605 455
BsaHI Gr'CG_yC
Cuts at: 1528 1776 2478 3974 1528
Size: 248 702 1496 1651
Fragments arranged by size:
1651 1496 702 248
BsaWI w'CCGG_w
Cuts at: 985 1286 1807 2485 2759 2906 3317 985
Size: 301 521 678 274 147 411 1765
Fragments arranged by size:
1765 678 521 411 301 274 147
BseYI C'CCAG_C
Cuts at: 155 321 756 840 1522 2280 2857 3353 155
Size: 166 435 84 682 758 577 496 899
Fragments arranged by size:
899 758 682 577 496 435 166 84
BsiEI CG_ry'CG
Cuts at: 104 1639 1685 2893 104
Size: 1535 46 1208 1308
Fragments arranged by size:
1535 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1111 1889 2079 2871 1111
Size: 778 190 792 2337
Fragments arranged by size:
2337 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 708 1030 1111 1722 1815 1889 2079 2141 2871
Size: 322 81 611 93 74 190 62 730
Cuts at: 2871 708
Size: 1934
Fragments arranged by size:
1934 730 611 322 190 93 81 74
62
BspEI T'CCGG_A
Cuts at: 985 3317 985
Size: 2332 1765
BspHI T'CATG_A
Cuts at: 263 3273 263
Size: 3010 1087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1147 1361 1663 2044 1147
Size: 214 302 381 3200
Fragments arranged by size:
3200 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2389 2443 3927 4092 50
Size: 2339 54 1484 165 55
Fragments arranged by size:
2339 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 530 2009 530
Size: 1479 2618
BsrFI r'CCGG_y
Cuts at: 2095 2276 2485 2095
Size: 181 209 3707
Fragments arranged by size:
3707 209 181
BssSI C'ACGA_G
Cuts at: 2368 2726 2368
Size: 358 3739
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1474
Size: 4097
BstBI TT'CG_AA
Cuts at: 2458 2458
Size: 4097
BstXI CCAn_nnnn'nTGG
Cuts at: 774 774
Size: 4097
BstYI r'GATC_y
Cuts at: 1 270 1122 1589 1612 1947 2193 3205 3291
Size: 269 852 467 23 335 246 1012 86
Cuts at: 3291 3303 3649 1
Size: 12 346 449
Fragments arranged by size:
1012 852 467 449 346 335 269 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 725 789 1041 107
Size: 618 64 252 3163
Fragments arranged by size:
3163 618 252 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2047 2228 2391 2047
Size: 181 163 3753
Fragments arranged by size:
3753 181 163
BtsI GCAGTG_nn'
Cuts at: 1340 1340
Size: 4097
DraI TTT'AAA
Cuts at: 1096 1096
Size: 4097
DrdI GACnn_nn'nnGTC
Cuts at: 1803 2661 1803
Size: 858 3239
EaeI y'GGCC_r
Cuts at: 786 1010 1038 1682 1856 2247 2274 3619 786
Size: 224 28 644 174 391 27 1345 1264
Fragments arranged by size:
1345 1264 644 391 224 174 28 27
EagI C'GGCC_G
Cuts at: 1682 1682
Size: 4097
EarI CTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2305 2615 2761 3907 2305
Size: 310 146 1146 2495
Fragments arranged by size:
2495 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1109 1109
Size: 4097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 1922 2354 3101 3369 273
Size: 1649 432 747 268 1001
Fragments arranged by size:
1649 1001 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 946 1236 946
Size: 290 3807
EcoRI G'AATT_C
Cuts at: 1101 1101
Size: 4097
EcoRV GAT'ATC
Cuts at: 763 763
Size: 4097
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2116 2116
Size: 4097
FspI TGC'GCA
Cuts at: 1878 1878
Size: 4097
HaeII r_GCGC'y
Cuts at: 146 1531 1779 2801 3780 146
Size: 1385 248 1022 979 463
Fragments arranged by size:
1385 1022 979 463 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1939 1969 1971 2001 1939
Size: 30 2 30 4035
Fragments arranged by size:
4035 30 30 2
HincII GTy'rAC
Cuts at: 1136 1136
Size: 4097
KasI G'GCGC_C
Cuts at: 1527 1775 1527
Size: 248 3849
KpnI G_GTAC'C
Cuts at: 1117 1117
Size: 4097
MluI A'CGCG_T
Cuts at: 731 3530 731
Size: 2799 1298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 926 2768 2952 926
Size: 1842 184 2071
Fragments arranged by size:
2071 1842 184
MscI TGG'CCA
Cuts at: 1858 1858
Size: 4097
MslI CAynn'nnrTG
Cuts at: 472 623 751 2086 2213 472
Size: 151 128 1335 127 2356
Fragments arranged by size:
2356 1335 151 128 127
MspA1I CmG'CkG
Cuts at: 847 871 1208 1522 1882 2895 3140 847
Size: 24 337 314 360 1013 245 1804
Fragments arranged by size:
1804 1013 360 337 314 245 24
NaeI GCC'GGC
Cuts at: 2278 2278
Size: 4097
NarI GG'CG_CC
Cuts at: 1528 1776 1528
Size: 248 3849
NcoI C'CATG_G
Cuts at: 107 725 107
Size: 618 3479
NgoMIV G'CCGG_C
Cuts at: 2276 2276
Size: 4097
NspI r_CATG'y
Cuts at: 1150 1650 2557 1150
Size: 500 907 2690
Fragments arranged by size:
2690 907 500
PciI A'CATG_T
Cuts at: 2553 2553
Size: 4097
PflMI CCAn_nnn'nTGG
Cuts at: 817 817
Size: 4097
PmeI GTTT'AAAC
Cuts at: 1096 1096
Size: 4097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 4065
PpuMI rG'GwC_Cy
Cuts at: 946 946
Size: 4097
PspOMI G'GGCC_C
Cuts at: 704 704
Size: 4097
PstI C_TGCA'G
Cuts at: 1144 1829 1144
Size: 685 3412
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4097
PvuII CAG'CTG
Cuts at: 1522 1882 1522
Size: 360 3737
RsrII CG'GwC_CG
Cuts at: 2292 2292
Size: 4097
SacI G_AGCT'C
Cuts at: 1111 1111
Size: 4097
SalI G'TCGA_C
Cuts at: 1134 1134
Size: 4097
SapI GCTCTTCn'nnn_
Cuts at: 2120 2330 2120
Size: 210 3887
SbfI CC_TGCA'GG
Cuts at: 1144 1144
Size: 4097
ScaI AGT'ACT
Cuts at: 3614 3614
Size: 4097
SfcI C'TryA_G
Cuts at: 33 1140 1825 2818 3009 33
Size: 1107 685 993 191 1121
Fragments arranged by size:
1121 1107 993 685 191
SfoI GGC'GCC
Cuts at: 1529 1777 1529
Size: 248 3849
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4097
SmaI CCC'GGG
Cuts at: 709 929 1119 709
Size: 220 190 3687
Fragments arranged by size:
3687 220 190
SmlI C'TyrA_G
Cuts at: 1252 2167 2659 2921 1252
Size: 915 492 262 2428
Fragments arranged by size:
2428 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4097
SphI G_CATG'C
Cuts at: 1150 1650 1150
Size: 500 3597
SspI AAT'ATT
Cuts at: 443 493 443
Size: 50 4047
StyI C'CwwG_G
Cuts at: 107 126 725 1231 1585 107
Size: 19 599 506 354 2619
Fragments arranged by size:
2619 599 506 354 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1680 1813 2272 2481 1680
Size: 133 459 209 3296
Fragments arranged by size:
3296 459 209 133
TatI w'GTAC_w
Cuts at: 3612 3612
Size: 4097
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4065
Tth111I GACn'n_nGTC
Cuts at: 1894 1894
Size: 4097
XbaI T'CTAG_A
Cuts at: 1128 1128
Size: 4097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1048 1592 1048
Size: 544 3553
XmaI C'CCGG_G
Cuts at: 707 927 1117 707
Size: 220 190 3687
Fragments arranged by size:
3687 220 190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI