Restriction map for
pF1KE0753
(Circular) (Six-base) MAPSORT of: pF1KE0753.seq Check: 989 from: 1 to: 4127
pF1KE0753 4127 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 401 1165 401
Size: 764 3363
Acc65I G'GTAC_C
Cuts at: 1143 1143
Size: 4127
AclI AA'CG_TT
Cuts at: 3967 3967
Size: 4127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1952 2384 3131 3399 1952
Size: 432 747 268 2680
Fragments arranged by size:
2680 747 432 268
AfeI AGC'GCT
Cuts at: 454 454
Size: 4127
AflIII A'CryG_T
Cuts at: 1479 2583 3560 1479
Size: 1104 977 2046
Fragments arranged by size:
2046 1104 977
AgeI A'CCGG_T
Cuts at: 2515 2515
Size: 4127
AleI CACnn'nnGTG
Cuts at: 832 832
Size: 4127
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 694 726 694
Size: 32 4095
AlwNI CAG_nnn'CTG
Cuts at: 1432 2999 1432
Size: 1567 2560
ApaLI G'TGCA_C
Cuts at: 541 2897 541
Size: 2356 1771
ApoI r'AATT_y
Cuts at: 1131 3480 3736 1131
Size: 2349 256 1522
Fragments arranged by size:
2349 1522 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
AvaI C'yCGr_G
Cuts at: 395 1147 395
Size: 752 3375
BamHI G'GATC_C
Cuts at: 446 1152 446
Size: 706 3421
BanI G'GyrC_C
Cuts at: 654 1143 1557 1805 1840 654
Size: 489 414 248 35 2941
Fragments arranged by size:
2941 489 414 248 35
BanII G_rGCy'C
Cuts at: 476 1141 2171 476
Size: 665 1030 2432
Fragments arranged by size:
2432 1030 665
BbeI G_GCGC'C
Cuts at: 1561 1809 1561
Size: 248 3879
BbvCI CC'TCA_GC
Cuts at: 718 718
Size: 4127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 519 553 519
Size: 34 4093
BciVI GTATCCnnnnn_n'
Cuts at: 2025 2786 4127 2025
Size: 761 1341 2025
Fragments arranged by size:
2025 1341 761
BclI T'GATC_A
Cuts at: 1647 1647
Size: 4127
BglII A'GATC_T
Cuts at: 761 1642 3679 761
Size: 881 2037 1209
Fragments arranged by size:
2037 1209 881
BlpI GC'TnA_GC
Cuts at: 1239 1239
Size: 4127
Bme1580I G_kGCm'C
Cuts at: 545 1752 1845 2901 545
Size: 1207 93 1056 1771
Fragments arranged by size:
1771 1207 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1339 1420 1755 3899 1339
Size: 81 335 2144 1567
Fragments arranged by size:
2144 1567 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 410 2494 410
Size: 2084 2043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 825 937 825
Size: 112 4015
Bpu10I CC'TnA_GC
Cuts at: 422 718 1031 422
Size: 296 313 3518
Fragments arranged by size:
3518 313 296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1081 1303 2182 2674 2972 1003
Size: 78 222 879 492 298 2158
Fragments arranged by size:
2158 879 492 298 222 78
BsaI GGTCTCn'nnnn_
Cuts at: 3831 3831
Size: 4127
BsaAI yAC'GTr
Cuts at: 1480 1480
Size: 4127
BsaBI GATnn'nnATC
Cuts at: 6 299 1641 3678 6
Size: 293 1342 2037 455
Fragments arranged by size:
2037 1342 455 293
BsaHI Gr'CG_yC
Cuts at: 815 1558 1806 2508 4004 815
Size: 743 248 702 1496 938
Fragments arranged by size:
1496 938 743 702 248
BsaWI w'CCGG_w
Cuts at: 1316 1837 2515 2789 2936 3347 1316
Size: 521 678 274 147 411 2096
Fragments arranged by size:
2096 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 583 613 583
Size: 30 4097
BseYI C'CCAG_C
Cuts at: 497 556 909 1552 2310 2887 3383 497
Size: 59 353 643 758 577 496 1241
Fragments arranged by size:
1241 758 643 577 496 353 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 508
Size: 4127
BsiEI CG_ry'CG
Cuts at: 104 1121 1669 1715 2923 104
Size: 1017 548 46 1208 1308
Fragments arranged by size:
1308 1208 1017 548 46
BsiHKAI G_wGCw'C
Cuts at: 391 545 1019 1141 1919 2109 2901 391
Size: 154 474 122 778 190 792 1617
Fragments arranged by size:
1617 792 778 474 190 154 122
Bsp1286I G_dGCh'C
Cuts at: 391 476 545 1019 1141 1752 1845 1919 2109
Size: 85 69 474 122 611 93 74 190
Cuts at: 2109 2171 2901 391
Size: 62 730 1617
Fragments arranged by size:
1617 730 611 474 190 122 93 85
74 69 62
BspEI T'CCGG_A
Cuts at: 3347 3347
Size: 4127
BspHI T'CATG_A
Cuts at: 3303 3303
Size: 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 530 1177 1391 1693 2074 530
Size: 647 214 302 381 2583
Fragments arranged by size:
2583 647 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2419 2473 3957 4122 50
Size: 2369 54 1484 165 55
Fragments arranged by size:
2369 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 512 2039 512
Size: 1527 2600
BsrFI r'CCGG_y
Cuts at: 2125 2306 2515 2125
Size: 181 209 3737
Fragments arranged by size:
3737 209 181
BssSI C'ACGA_G
Cuts at: 2398 2756 2398
Size: 358 3769
BstAPI GCAn_nnn'nTGC
Cuts at: 1504 1504
Size: 4127
BstBI TT'CG_AA
Cuts at: 2488 2488
Size: 4127
BstXI CCAn_nnnn'nTGG
Cuts at: 990 990
Size: 4127
BstYI r'GATC_y
Cuts at: 1 446 761 1152 1619 1642 1977 2223 3235
Size: 445 315 391 467 23 335 246 1012
Cuts at: 3235 3321 3333 3679 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 445 391 346 335 315
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 402 402
Size: 4127
BtgI C'CryG_G
Cuts at: 107 204 107
Size: 97 4030
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2077 2258 2421 2077
Size: 181 163 3783
Fragments arranged by size:
3783 181 163
BtsI GCAGTG_nn'
Cuts at: 1370 1370
Size: 4127
DraI TTT'AAA
Cuts at: 1126 1126
Size: 4127
DrdI GACnn_nn'nnGTC
Cuts at: 1833 2691 1833
Size: 858 3269
EaeI y'GGCC_r
Cuts at: 201 1712 1886 2277 2304 3649 201
Size: 1511 174 391 27 1345 679
Fragments arranged by size:
1511 1345 679 391 174 27
EagI C'GGCC_G
Cuts at: 1712 1712
Size: 4127
EarI CTCTTCn'nnn_
Cuts at: 2150 2360 2150
Size: 210 3917
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2335 2645 2791 3937 2335
Size: 310 146 1146 2525
Fragments arranged by size:
2525 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1139 1139
Size: 4127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 825 937 1952 2384 3131 3399 825
Size: 112 1015 432 747 268 1553
Fragments arranged by size:
1553 1015 747 432 268 112
EcoO109I rG'GnC_Cy
Cuts at: 461 1266 461
Size: 805 3322
EcoRI G'AATT_C
Cuts at: 1131 1131
Size: 4127
EcoRV GAT'ATC
Cuts at: 629 629
Size: 4127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 4127
FspI TGC'GCA
Cuts at: 1908 1908
Size: 4127
HaeII r_GCGC'y
Cuts at: 456 1561 1809 2831 3810 456
Size: 1105 248 1022 979 773
Fragments arranged by size:
1105 1022 979 773 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 376 389 408 421 1969 1999 2001 2031 376
Size: 13 19 13 1548 30 2 30 2472
Fragments arranged by size:
2472 1548 30 30 19 13 13 2
HincII GTy'rAC
Cuts at: 1166 1166
Size: 4127
KasI G'GCGC_C
Cuts at: 1557 1805 1557
Size: 248 3879
KpnI G_GTAC'C
Cuts at: 1147 1147
Size: 4127
MluI A'CGCG_T
Cuts at: 3560 3560
Size: 4127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 569 2798 2982 569
Size: 2229 184 1714
Fragments arranged by size:
2229 1714 184
MscI TGG'CCA
Cuts at: 203 1888 203
Size: 1685 2442
MslI CAynn'nnrTG
Cuts at: 832 2116 2243 832
Size: 1284 127 2716
Fragments arranged by size:
2716 1284 127
MspA1I CmG'CkG
Cuts at: 501 1238 1552 1912 2925 3170 501
Size: 737 314 360 1013 245 1458
Fragments arranged by size:
1458 1013 737 360 314 245
NaeI GCC'GGC
Cuts at: 2308 2308
Size: 4127
NarI GG'CG_CC
Cuts at: 1558 1806 1558
Size: 248 3879
NcoI C'CATG_G
Cuts at: 107 204 107
Size: 97 4030
NgoMIV G'CCGG_C
Cuts at: 2306 2306
Size: 4127
NspI r_CATG'y
Cuts at: 1180 1680 2587 1180
Size: 500 907 2720
Fragments arranged by size:
2720 907 500
PasI CC'CwG_GG
Cuts at: 507 507
Size: 4127
PciI A'CATG_T
Cuts at: 2583 2583
Size: 4127
PflMI CCAn_nnn'nTGG
Cuts at: 672 672
Size: 4127
PmeI GTTT'AAAC
Cuts at: 1126 1126
Size: 4127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 4095
PstI C_TGCA'G
Cuts at: 1174 1859 1174
Size: 685 3442
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4127
PvuII CAG'CTG
Cuts at: 501 1552 1912 501
Size: 1051 360 2716
Fragments arranged by size:
2716 1051 360
RsrII CG'GwC_CG
Cuts at: 2322 2322
Size: 4127
SacI G_AGCT'C
Cuts at: 1141 1141
Size: 4127
SalI G'TCGA_C
Cuts at: 1164 1164
Size: 4127
SapI GCTCTTCn'nnn_
Cuts at: 2150 2360 2150
Size: 210 3917
SbfI CC_TGCA'GG
Cuts at: 1174 1174
Size: 4127
ScaI AGT'ACT
Cuts at: 3644 3644
Size: 4127
SfcI C'TryA_G
Cuts at: 33 1170 1855 2848 3039 33
Size: 1137 685 993 191 1121
Fragments arranged by size:
1137 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1559 1807 1559
Size: 248 3879
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
SmaI CCC'GGG
Cuts at: 1149 1149
Size: 4127
SmlI C'TyrA_G
Cuts at: 395 1018 1060 1282 2197 2689 2951 395
Size: 623 42 222 915 492 262 1571
Fragments arranged by size:
1571 915 623 492 262 222 42
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4127
SphI G_CATG'C
Cuts at: 1180 1680 1180
Size: 500 3627
StuI AGG'CCT
Cuts at: 289 289
Size: 4127
StyI C'CwwG_G
Cuts at: 107 204 1261 1615 107
Size: 97 1057 354 2619
Fragments arranged by size:
2619 1057 354 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1002 1057 1710 1843 2302 2511 1002
Size: 55 653 133 459 209 2618
Fragments arranged by size:
2618 653 459 209 133 55
TatI w'GTAC_w
Cuts at: 3642 3642
Size: 4127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3644 3676 3644
Size: 32 4095
Tth111I GACn'n_nGTC
Cuts at: 1924 1924
Size: 4127
XbaI T'CTAG_A
Cuts at: 1158 1158
Size: 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 211 730 1622 211
Size: 519 892 2716
Fragments arranged by size:
2716 892 519
XhoI C'TCGA_G
Cuts at: 395 395
Size: 4127
XmaI C'CCGG_G
Cuts at: 1147 1147
Size: 4127
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI AvrII
BaeI BbsI BfrBI BglI BmgBI BmtI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstEII Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI