Restriction map for
pF1KE0755
(Circular) (Six-base) MAPSORT of: pF1KE0755.seq Check: 8443 from: 1 to: 4100
pF1KE0755 4100 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1138 1138
Size: 4100
Acc65I G'GTAC_C
Cuts at: 1116 1116
Size: 4100
AclI AA'CG_TT
Cuts at: 3940 3940
Size: 4100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 967 1925 2357 3104 3372 655
Size: 312 958 432 747 268 1383
Fragments arranged by size:
1383 958 747 432 312 268
AflIII A'CryG_T
Cuts at: 1452 2556 3533 1452
Size: 1104 977 2019
Fragments arranged by size:
2019 1104 977
AgeI A'CCGG_T
Cuts at: 382 2488 382
Size: 2106 1994
AhdI GACnn_n'nnGTC
Cuts at: 339 339
Size: 4100
AleI CACnn'nnGTG
Cuts at: 881 881
Size: 4100
AlwNI CAG_nnn'CTG
Cuts at: 295 868 1405 2972 295
Size: 573 537 1567 1423
Fragments arranged by size:
1567 1423 573 537
ApaI G_GGCC'C
Cuts at: 457 664 457
Size: 207 3893
ApaLI G'TGCA_C
Cuts at: 2870 2870
Size: 4100
ApoI r'AATT_y
Cuts at: 1104 3453 3709 1104
Size: 2349 256 1495
Fragments arranged by size:
2349 1495 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4100
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
AvaI C'yCGr_G
Cuts at: 374 477 1120 374
Size: 103 643 3354
Fragments arranged by size:
3354 643 103
AvrII C'CTAG_G
Cuts at: 994 994
Size: 4100
BamHI G'GATC_C
Cuts at: 502 1125 502
Size: 623 3477
BanI G'GyrC_C
Cuts at: 385 694 986 1116 1530 1778 1813 385
Size: 309 292 130 414 248 35 2672
Fragments arranged by size:
2672 414 309 292 248 130 35
BanII G_rGCy'C
Cuts at: 198 457 664 1036 1114 2144 198
Size: 259 207 372 78 1030 2154
Fragments arranged by size:
2154 1030 372 259 207 78
BbeI G_GCGC'C
Cuts at: 990 1534 1782 990
Size: 544 248 3308
Fragments arranged by size:
3308 544 248
BbsI GAAGACnn'nnnn_
Cuts at: 955 955
Size: 4100
BbvCI CC'TCA_GC
Cuts at: 679 1068 679
Size: 389 3711
BciVI GTATCCnnnnn_n'
Cuts at: 1998 2759 4100 1998
Size: 761 1341 1998
Fragments arranged by size:
1998 1341 761
BclI T'GATC_A
Cuts at: 1620 1620
Size: 4100
BglI GCCn_nnn'nGGC
Cuts at: 221 300 1031 221
Size: 79 731 3290
Fragments arranged by size:
3290 731 79
BglII A'GATC_T
Cuts at: 1615 3652 1615
Size: 2037 2063
BlpI GC'TnA_GC
Cuts at: 431 1212 431
Size: 781 3319
Bme1580I G_kGCm'C
Cuts at: 457 664 697 1725 1818 2874 457
Size: 207 33 1028 93 1056 1683
Fragments arranged by size:
1683 1056 1028 207 93 33
BmrI ACTGGGnnnn_n'
Cuts at: 510 1312 1393 1728 3872 510
Size: 802 81 335 2144 738
Fragments arranged by size:
2144 802 738 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 513 570 2467 513
Size: 57 1897 2146
Fragments arranged by size:
2146 1897 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 256 1012 139
Size: 117 756 3227
Fragments arranged by size:
3227 756 117
Bpu10I CC'TnA_GC
Cuts at: 679 1068 679
Size: 389 3711
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 2155 2647 2945 1276
Size: 879 492 298 2431
Fragments arranged by size:
2431 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 115 3804 115
Size: 3689 411
BsaAI yAC'GTr
Cuts at: 1453 1453
Size: 4100
BsaBI GATnn'nnATC
Cuts at: 6 1614 3651 6
Size: 1608 2037 455
Fragments arranged by size:
2037 1608 455
BsaHI Gr'CG_yC
Cuts at: 668 773 987 1531 1779 2481 3977 668
Size: 105 214 544 248 702 1496 791
Fragments arranged by size:
1496 791 702 544 248 214 105
BsaWI w'CCGG_w
Cuts at: 382 1289 1810 2488 2762 2909 3320 382
Size: 907 521 678 274 147 411 1162
Fragments arranged by size:
1162 907 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1045 1075 1045
Size: 30 4070
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 486 555 772 486
Size: 69 217 3814
Fragments arranged by size:
3814 217 69
BseYI C'CCAG_C
Cuts at: 242 657 868 1044 1525 2283 2860 3356 242
Size: 415 211 176 481 758 577 496 986
Fragments arranged by size:
986 758 577 496 481 415 211 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 1058 622
Size: 436 3664
BsiEI CG_ry'CG
Cuts at: 104 1642 1688 2896 104
Size: 1538 46 1208 1308
Fragments arranged by size:
1538 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1114 1892 2082 2874 1114
Size: 778 190 792 2340
Fragments arranged by size:
2340 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 198 457 664 697 1036 1114 1725 1818 1892
Size: 259 207 33 339 78 611 93 74
Cuts at: 1892 2082 2144 2874 198
Size: 190 62 730 1424
Fragments arranged by size:
1424 730 611 339 259 207 190 93
78 74 62 33
BspEI T'CCGG_A
Cuts at: 3320 3320
Size: 4100
BspHI T'CATG_A
Cuts at: 3276 3276
Size: 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1150 1364 1666 2047 1150
Size: 214 302 381 3203
Fragments arranged by size:
3203 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2392 2446 3930 4095 50
Size: 2342 54 1484 165 55
Fragments arranged by size:
2342 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2012 2012
Size: 4100
BsrFI r'CCGG_y
Cuts at: 299 351 382 642 2098 2279 2488 299
Size: 52 31 260 1456 181 209 1911
Fragments arranged by size:
1911 1456 260 209 181 52 31
BssSI C'ACGA_G
Cuts at: 2371 2729 2371
Size: 358 3742
BstAPI GCAn_nnn'nTGC
Cuts at: 176 1477 176
Size: 1301 2799
BstBI TT'CG_AA
Cuts at: 2461 2461
Size: 4100
BstYI r'GATC_y
Cuts at: 1 502 1125 1592 1615 1950 2196 3208 3294
Size: 501 623 467 23 335 246 1012 86
Cuts at: 3294 3306 3652 1
Size: 12 346 449
Fragments arranged by size:
1012 623 501 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 498 498
Size: 4100
BtgI C'CryG_G
Cuts at: 107 542 593 107
Size: 435 51 3614
Fragments arranged by size:
3614 435 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2050 2231 2394 2050
Size: 181 163 3756
Fragments arranged by size:
3756 181 163
BtsI GCAGTG_nn'
Cuts at: 1343 1343
Size: 4100
DraI TTT'AAA
Cuts at: 1099 1099
Size: 4100
DrdI GACnn_nn'nnGTC
Cuts at: 1806 2664 1806
Size: 858 3242
EaeI y'GGCC_r
Cuts at: 297 1685 1859 2250 2277 3622 297
Size: 1388 174 391 27 1345 775
Fragments arranged by size:
1388 1345 775 391 174 27
EagI C'GGCC_G
Cuts at: 1685 1685
Size: 4100
EarI CTCTTCn'nnn_
Cuts at: 2123 2333 2123
Size: 210 3890
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 198 313 2308 2618 2764 3910 198
Size: 115 1995 310 146 1146 388
Fragments arranged by size:
1995 1146 388 310 146 115
EcoICRI GAG'CTC
Cuts at: 1112 1112
Size: 4100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 256 655 967 1012 1925 2357 3104 3372
Size: 117 399 312 45 913 432 747 268
Cuts at: 3372 139
Size: 867
Fragments arranged by size:
913 867 747 432 399 312 268 117
45
EcoO109I rG'GnC_Cy
Cuts at: 292 454 531 702 1239 292
Size: 162 77 171 537 3153
Fragments arranged by size:
3153 537 171 162 77
EcoRI G'AATT_C
Cuts at: 1104 1104
Size: 4100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 4100
FseI GG_CCGG'CC
Cuts at: 646 646
Size: 4100
FspI TGC'GCA
Cuts at: 733 1881 733
Size: 1148 2952
HaeII r_GCGC'y
Cuts at: 869 990 1534 1782 2804 3783 869
Size: 121 544 248 1022 979 1186
Fragments arranged by size:
1186 1022 979 544 248 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 305 325 337 357 502 534 799 831 1942
Size: 20 12 20 145 32 265 32 1111
Cuts at: 1942 1972 1974 2004 305
Size: 30 2 30 2401
Fragments arranged by size:
2401 1111 265 145 32 32 30 30
20 20 12 2
HincII GTy'rAC
Cuts at: 1139 1139
Size: 4100
KasI G'GCGC_C
Cuts at: 986 1530 1778 986
Size: 544 248 3308
Fragments arranged by size:
3308 544 248
KpnI G_GTAC'C
Cuts at: 1120 1120
Size: 4100
MluI A'CGCG_T
Cuts at: 3533 3533
Size: 4100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 770 2771 2955 770
Size: 2001 184 1915
Fragments arranged by size:
2001 1915 184
MscI TGG'CCA
Cuts at: 1861 1861
Size: 4100
MslI CAynn'nnrTG
Cuts at: 881 2089 2216 881
Size: 1208 127 2765
Fragments arranged by size:
2765 1208 127
MspA1I CmG'CkG
Cuts at: 274 468 626 633 678 713 1072 1211 1525
Size: 194 158 7 45 35 359 139 314
Cuts at: 1525 1885 2898 3143 274
Size: 360 1013 245 1231
Fragments arranged by size:
1231 1013 360 359 314 245 194 158
139 45 35 7
NaeI GCC'GGC
Cuts at: 301 353 644 2281 301
Size: 52 291 1637 2120
Fragments arranged by size:
2120 1637 291 52
NarI GG'CG_CC
Cuts at: 987 1531 1779 987
Size: 544 248 3308
Fragments arranged by size:
3308 544 248
NcoI C'CATG_G
Cuts at: 107 542 107
Size: 435 3665
NgoMIV G'CCGG_C
Cuts at: 299 351 642 2279 299
Size: 52 291 1637 2120
Fragments arranged by size:
2120 1637 291 52
NspI r_CATG'y
Cuts at: 160 1153 1653 2560 160
Size: 993 500 907 1700
Fragments arranged by size:
1700 993 907 500
PciI A'CATG_T
Cuts at: 2556 2556
Size: 4100
PflMI CCAn_nnn'nTGG
Cuts at: 423 868 423
Size: 445 3655
PfoI T'CCnGG_A
Cuts at: 251 911 251
Size: 660 3440
PmeI GTTT'AAAC
Cuts at: 1099 1099
Size: 4100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 4068
PspOMI G'GGCC_C
Cuts at: 453 660 453
Size: 207 3893
PstI C_TGCA'G
Cuts at: 1147 1832 1147
Size: 685 3415
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4100
PvuII CAG'CTG
Cuts at: 468 633 678 713 1072 1525 1885 468
Size: 165 45 35 359 453 360 2683
Fragments arranged by size:
2683 453 360 359 165 45 35
RsrII CG'GwC_CG
Cuts at: 2295 2295
Size: 4100
SacI G_AGCT'C
Cuts at: 1114 1114
Size: 4100
SalI G'TCGA_C
Cuts at: 1137 1137
Size: 4100
SapI GCTCTTCn'nnn_
Cuts at: 2123 2333 2123
Size: 210 3890
SbfI CC_TGCA'GG
Cuts at: 1147 1147
Size: 4100
ScaI AGT'ACT
Cuts at: 3617 3617
Size: 4100
SfcI C'TryA_G
Cuts at: 33 708 1143 1828 2821 3012 33
Size: 675 435 685 993 191 1121
Fragments arranged by size:
1121 993 685 675 435 191
SfoI GGC'GCC
Cuts at: 988 1532 1780 988
Size: 544 248 3308
Fragments arranged by size:
3308 544 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
SgrAI Cr'CCGG_yG
Cuts at: 382 382
Size: 4100
SmaI CCC'GGG
Cuts at: 376 479 1122 376
Size: 103 643 3354
Fragments arranged by size:
3354 643 103
SmlI C'TyrA_G
Cuts at: 1255 2170 2662 2924 1255
Size: 915 492 262 2431
Fragments arranged by size:
2431 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4100
SphI G_CATG'C
Cuts at: 1153 1653 1153
Size: 500 3600
StuI AGG'CCT
Cuts at: 445 493 445
Size: 48 4052
StyI C'CwwG_G
Cuts at: 107 542 689 897 994 1083 1234 1588 107
Size: 435 147 208 97 89 151 354 2619
Fragments arranged by size:
2619 435 354 208 151 147 97 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 712 1683 1816 2275 2484 712
Size: 971 133 459 209 2328
Fragments arranged by size:
2328 971 459 209 133
TatI w'GTAC_w
Cuts at: 3615 3615
Size: 4100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1045 1077 3617 3649 1045
Size: 32 2540 32 1496
Fragments arranged by size:
2540 1496 32 32
Tth111I GACn'n_nGTC
Cuts at: 1897 1897
Size: 4100
XbaI T'CTAG_A
Cuts at: 1131 1131
Size: 4100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 465 531 1595 114
Size: 351 66 1064 2619
Fragments arranged by size:
2619 1064 351 66
XmaI C'CCGG_G
Cuts at: 374 477 1120 374
Size: 103 643 3354
Fragments arranged by size:
3354 643 103
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI