Restriction map for
pF1KE0764
(Circular) (Six-base) MAPSORT of: pF1KE0764.seq Check: 6381 from: 1 to: 4424
pF1KE0764 4424 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1462 1462
Size: 4424
Acc65I G'GTAC_C
Cuts at: 1440 1440
Size: 4424
AclI AA'CG_TT
Cuts at: 823 4264 823
Size: 3441 983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 821 1052 2249 2681 3428 3696 334
Size: 487 231 1197 432 747 268 1062
Fragments arranged by size:
1197 1062 747 487 432 268 231
AflIII A'CryG_T
Cuts at: 494 1776 2880 3857 494
Size: 1282 1104 977 1061
Fragments arranged by size:
1282 1104 1061 977
AgeI A'CCGG_T
Cuts at: 2812 2812
Size: 4424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 426 426
Size: 4424
AlwNI CAG_nnn'CTG
Cuts at: 1245 1729 3296 1245
Size: 484 1567 2373
Fragments arranged by size:
2373 1567 484
ApaLI G'TGCA_C
Cuts at: 3194 3194
Size: 4424
ApoI r'AATT_y
Cuts at: 1410 1428 3777 4033 1410
Size: 18 2349 256 1801
Fragments arranged by size:
2349 1801 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 4424
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
AvaI C'yCGr_G
Cuts at: 1444 1444
Size: 4424
BamHI G'GATC_C
Cuts at: 1449 1449
Size: 4424
BanI G'GyrC_C
Cuts at: 1440 1854 2102 2137 1440
Size: 414 248 35 3727
Fragments arranged by size:
3727 414 248 35
BanII G_rGCy'C
Cuts at: 1438 2468 1438
Size: 1030 3394
BbeI G_GCGC'C
Cuts at: 1858 2106 1858
Size: 248 4176
BbsI GAAGACnn'nnnn_
Cuts at: 727 727
Size: 4424
BciVI GTATCCnnnnn_n'
Cuts at: 2322 3083 4424 2322
Size: 761 1341 2322
Fragments arranged by size:
2322 1341 761
BclI T'GATC_A
Cuts at: 1944 1944
Size: 4424
BglII A'GATC_T
Cuts at: 219 1939 3976 219
Size: 1720 2037 667
Fragments arranged by size:
2037 1720 667
BlpI GC'TnA_GC
Cuts at: 1536 1536
Size: 4424
Bme1580I G_kGCm'C
Cuts at: 2049 2142 3198 2049
Size: 93 1056 3275
Fragments arranged by size:
3275 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1636 1717 2052 4196 1636
Size: 81 335 2144 1864
Fragments arranged by size:
2144 1864 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 191 2791 191
Size: 2600 1824
Bpu10I CC'TnA_GC
Cuts at: 280 280
Size: 4424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 1363 1600 2479 2971 3269 161
Size: 1202 237 879 492 298 1316
Fragments arranged by size:
1316 1202 879 492 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 4128 4128
Size: 4424
BsaAI yAC'GTr
Cuts at: 1777 1777
Size: 4424
BsaBI GATnn'nnATC
Cuts at: 6 1938 3975 6
Size: 1932 2037 455
Fragments arranged by size:
2037 1932 455
BsaHI Gr'CG_yC
Cuts at: 1855 2103 2805 4301 1855
Size: 248 702 1496 1978
Fragments arranged by size:
1978 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1613 2134 2812 3086 3233 3644 1613
Size: 521 678 274 147 411 2393
Fragments arranged by size:
2393 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1039 1069 1039
Size: 30 4394
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 216 605 1076 216
Size: 389 471 3564
Fragments arranged by size:
3564 471 389
BseYI C'CCAG_C
Cuts at: 670 727 1849 2607 3184 3680 670
Size: 57 1122 758 577 496 1414
Fragments arranged by size:
1414 1122 758 577 496 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 629 502
Size: 127 4297
BsiEI CG_ry'CG
Cuts at: 104 1966 2012 3220 104
Size: 1862 46 1208 1308
Fragments arranged by size:
1862 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 141 1438 2216 2406 3198 141
Size: 1297 778 190 792 1367
Fragments arranged by size:
1367 1297 792 778 190
BsmI GAATG_Cn'
Cuts at: 415 415
Size: 4424
Bsp1286I G_dGCh'C
Cuts at: 141 1438 2049 2142 2216 2406 2468 3198 141
Size: 1297 611 93 74 190 62 730 1367
Fragments arranged by size:
1367 1297 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3644 3644
Size: 4424
BspHI T'CATG_A
Cuts at: 960 1292 3600 960
Size: 332 2308 1784
Fragments arranged by size:
2308 1784 332
BspMI ACCTGCnnnn'nnnn_
Cuts at: 386 1474 1688 1990 2371 386
Size: 1088 214 302 381 2439
Fragments arranged by size:
2439 1088 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2716 2770 4254 4419 50
Size: 2666 54 1484 165 55
Fragments arranged by size:
2666 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2336 2336
Size: 4424
BsrFI r'CCGG_y
Cuts at: 2422 2603 2812 2422
Size: 181 209 4034
Fragments arranged by size:
4034 209 181
BsrGI T'GTAC_A
Cuts at: 1055 1055
Size: 4424
BssSI C'ACGA_G
Cuts at: 2695 3053 2695
Size: 358 4066
BstAPI GCAn_nnn'nTGC
Cuts at: 1801 1801
Size: 4424
BstBI TT'CG_AA
Cuts at: 2785 2785
Size: 4424
BstYI r'GATC_y
Cuts at: 1 219 582 1449 1916 1939 2274 2520 3532
Size: 218 363 867 467 23 335 246 1012
Cuts at: 3532 3618 3630 3976 1
Size: 86 12 346 449
Fragments arranged by size:
1012 867 467 449 363 346 335 246
218 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2374 2555 2718 2374
Size: 181 163 4080
Fragments arranged by size:
4080 181 163
BtsI GCAGTG_nn'
Cuts at: 141 1667 141
Size: 1526 2898
DraI TTT'AAA
Cuts at: 1423 1423
Size: 4424
DrdI GACnn_nn'nnGTC
Cuts at: 1026 2130 2988 1026
Size: 1104 858 2462
Fragments arranged by size:
2462 1104 858
EaeI y'GGCC_r
Cuts at: 1226 2009 2183 2574 2601 3946 1226
Size: 783 174 391 27 1345 1704
Fragments arranged by size:
1704 1345 783 391 174 27
EagI C'GGCC_G
Cuts at: 2009 2009
Size: 4424
EarI CTCTTCn'nnn_
Cuts at: 705 1010 2447 2657 705
Size: 305 1437 210 2472
Fragments arranged by size:
2472 1437 305 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2632 2942 3088 4234 2632
Size: 310 146 1146 2822
Fragments arranged by size:
2822 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1436 1436
Size: 4424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 821 1052 2249 2681 3428 3696 334
Size: 487 231 1197 432 747 268 1062
Fragments arranged by size:
1197 1062 747 487 432 268 231
EcoO109I rG'GnC_Cy
Cuts at: 876 1563 876
Size: 687 3737
EcoRI G'AATT_C
Cuts at: 1428 1428
Size: 4424
EcoRV GAT'ATC
Cuts at: 209 209
Size: 4424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4424
FspI TGC'GCA
Cuts at: 2205 2205
Size: 4424
HaeII r_GCGC'y
Cuts at: 1858 2106 3128 4107 1858
Size: 248 1022 979 2175
Fragments arranged by size:
2175 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 426 458 748 780 942 974 986 1018 2266
Size: 32 290 32 162 32 12 32 1248
Cuts at: 2266 2296 2298 2328 426
Size: 30 2 30 2522
Fragments arranged by size:
2522 1248 290 162 32 32 32 32
30 30 12 2
HincII GTy'rAC
Cuts at: 375 783 1463 375
Size: 408 680 3336
Fragments arranged by size:
3336 680 408
HpaI GTT'AAC
Cuts at: 783 783
Size: 4424
KasI G'GCGC_C
Cuts at: 1854 2102 1854
Size: 248 4176
KpnI G_GTAC'C
Cuts at: 1444 1444
Size: 4424
MluI A'CGCG_T
Cuts at: 3857 3857
Size: 4424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 178 3095 3279 178
Size: 2917 184 1323
Fragments arranged by size:
2917 1323 184
MscI TGG'CCA
Cuts at: 1228 2185 1228
Size: 957 3467
MslI CAynn'nnrTG
Cuts at: 408 1206 2413 2540 408
Size: 798 1207 127 2292
Fragments arranged by size:
2292 1207 798 127
MspA1I CmG'CkG
Cuts at: 472 1332 1535 1849 2209 3222 3467 472
Size: 860 203 314 360 1013 245 1429
Fragments arranged by size:
1429 1013 860 360 314 245 203
NaeI GCC'GGC
Cuts at: 2605 2605
Size: 4424
NarI GG'CG_CC
Cuts at: 1855 2103 1855
Size: 248 4176
NgoMIV G'CCGG_C
Cuts at: 2603 2603
Size: 4424
NspI r_CATG'y
Cuts at: 427 1477 1977 2884 427
Size: 1050 500 907 1967
Fragments arranged by size:
1967 1050 907 500
PciI A'CATG_T
Cuts at: 2880 2880
Size: 4424
PflMI CCAn_nnn'nTGG
Cuts at: 1245 1245
Size: 4424
PfoI T'CCnGG_A
Cuts at: 1310 1310
Size: 4424
PmeI GTTT'AAAC
Cuts at: 1423 1423
Size: 4424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4392
PstI C_TGCA'G
Cuts at: 1471 2156 1471
Size: 685 3739
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4424
PvuII CAG'CTG
Cuts at: 472 1849 2209 472
Size: 1377 360 2687
Fragments arranged by size:
2687 1377 360
RsrII CG'GwC_CG
Cuts at: 2619 2619
Size: 4424
SacI G_AGCT'C
Cuts at: 1438 1438
Size: 4424
SalI G'TCGA_C
Cuts at: 1461 1461
Size: 4424
SapI GCTCTTCn'nnn_
Cuts at: 2447 2657 2447
Size: 210 4214
SbfI CC_TGCA'GG
Cuts at: 1471 1471
Size: 4424
ScaI AGT'ACT
Cuts at: 3941 3941
Size: 4424
SfcI C'TryA_G
Cuts at: 33 1467 2152 3145 3336 33
Size: 1434 685 993 191 1121
Fragments arranged by size:
1434 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1856 2104 1856
Size: 248 4176
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
SmaI CCC'GGG
Cuts at: 1446 1446
Size: 4424
SmlI C'TyrA_G
Cuts at: 176 1378 1579 2494 2986 3248 176
Size: 1202 201 915 492 262 1352
Fragments arranged by size:
1352 1202 915 492 262 201
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4424
SphI G_CATG'C
Cuts at: 427 1477 1977 427
Size: 1050 500 2874
Fragments arranged by size:
2874 1050 500
StyI C'CwwG_G
Cuts at: 240 1558 1912 240
Size: 1318 354 2752
Fragments arranged by size:
2752 1318 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 294 684 741 1112 2007 2140 2599 2808 294
Size: 390 57 371 895 133 459 209 1910
Fragments arranged by size:
1910 895 459 390 371 209 133 57
TatI w'GTAC_w
Cuts at: 329 1055 3939 329
Size: 726 2884 814
Fragments arranged by size:
2884 814 726
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3941 3973 3941
Size: 32 4392
Tth111I GACn'n_nGTC
Cuts at: 633 2221 633
Size: 1588 2836
XbaI T'CTAG_A
Cuts at: 1455 1455
Size: 4424
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1919 1919
Size: 4424
XmaI C'CCGG_G
Cuts at: 1444 1444
Size: 4424
XmnI GAAnn'nnTTC
Cuts at: 298 298
Size: 4424
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI FspAI
HindIII MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI