Restriction map for
pF1KE0773
(Circular) (Six-base) MAPSORT of: pF1KE0773.seq Check: 2439 from: 1 to: 4145
pF1KE0773 4145 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 509 509
Size: 4145
AccI GT'mk_AC
Cuts at: 1183 1183
Size: 4145
Acc65I G'GTAC_C
Cuts at: 1161 1161
Size: 4145
AclI AA'CG_TT
Cuts at: 3985 3985
Size: 4145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 1970 2402 3149 3417 769
Size: 1201 432 747 268 1497
Fragments arranged by size:
1497 1201 747 432 268
AflIII A'CryG_T
Cuts at: 377 1497 2601 3578 377
Size: 1120 1104 977 944
Fragments arranged by size:
1120 1104 977 944
AgeI A'CCGG_T
Cuts at: 2533 2533
Size: 4145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 904 904
Size: 4145
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 964 996 964
Size: 32 4113
AlwNI CAG_nnn'CTG
Cuts at: 1450 3017 1450
Size: 1567 2578
ApaLI G'TGCA_C
Cuts at: 2915 2915
Size: 4145
ApoI r'AATT_y
Cuts at: 1149 3498 3754 1149
Size: 2349 256 1540
Fragments arranged by size:
2349 1540 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4145
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
AvaI C'yCGr_G
Cuts at: 389 1165 389
Size: 776 3369
BamHI G'GATC_C
Cuts at: 1170 1170
Size: 4145
BanI G'GyrC_C
Cuts at: 811 1161 1575 1823 1858 811
Size: 350 414 248 35 3098
Fragments arranged by size:
3098 414 350 248 35
BanII G_rGCy'C
Cuts at: 593 737 1008 1159 2189 593
Size: 144 271 151 1030 2549
Fragments arranged by size:
2549 1030 271 151 144
BbeI G_GCGC'C
Cuts at: 1579 1827 1579
Size: 248 3897
BbsI GAAGACnn'nnnn_
Cuts at: 805 805
Size: 4145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 465 499 465
Size: 34 4111
BciVI GTATCCnnnnn_n'
Cuts at: 2043 2804 4145 2043
Size: 761 1341 2043
Fragments arranged by size:
2043 1341 761
BclI T'GATC_A
Cuts at: 1665 1665
Size: 4145
BglII A'GATC_T
Cuts at: 1660 3697 1660
Size: 2037 2108
BlpI GC'TnA_GC
Cuts at: 1257 1257
Size: 4145
Bme1580I G_kGCm'C
Cuts at: 998 1770 1863 2919 998
Size: 772 93 1056 2224
Fragments arranged by size:
2224 1056 772 93
BmrI ACTGGGnnnn_n'
Cuts at: 1357 1438 1773 3917 1357
Size: 81 335 2144 1585
Fragments arranged by size:
2144 1585 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 4145
Bpu10I CC'TnA_GC
Cuts at: 169 1030 169
Size: 861 3284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 721 1321 2200 2692 2990 406
Size: 315 600 879 492 298 1561
Fragments arranged by size:
1561 879 600 492 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 940 1074 3849 940
Size: 134 2775 1236
Fragments arranged by size:
2775 1236 134
BsaAI yAC'GTr
Cuts at: 1498 1498
Size: 4145
BsaBI GATnn'nnATC
Cuts at: 6 487 1659 3696 6
Size: 481 1172 2037 455
Fragments arranged by size:
2037 1172 481 455
BsaHI Gr'CG_yC
Cuts at: 348 1576 1824 2526 4022 348
Size: 1228 248 702 1496 471
Fragments arranged by size:
1496 1228 702 471 248
BsaWI w'CCGG_w
Cuts at: 194 545 1334 1855 2533 2807 2954 3365 194
Size: 351 789 521 678 274 147 411 974
Fragments arranged by size:
974 789 678 521 411 351 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1003 1033 1003
Size: 30 4115
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 564 667 807 564
Size: 103 140 3902
Fragments arranged by size:
3902 140 103
BseYI C'CCAG_C
Cuts at: 182 1570 2328 2905 3401 182
Size: 1388 758 577 496 926
Fragments arranged by size:
1388 926 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 487 600 203
Size: 284 113 3748
Fragments arranged by size:
3748 284 113
BsiEI CG_ry'CG
Cuts at: 104 147 1687 1733 2941 104
Size: 43 1540 46 1208 1308
Fragments arranged by size:
1540 1308 1208 46 43
BsiHKAI G_wGCw'C
Cuts at: 175 593 1159 1937 2127 2919 175
Size: 418 566 778 190 792 1401
Fragments arranged by size:
1401 792 778 566 418 190
BsmBI CGTCTCn'nnnn_
Cuts at: 529 529
Size: 4145
Bsp1286I G_dGCh'C
Cuts at: 175 593 737 998 1008 1159 1770 1863 1937
Size: 418 144 261 10 151 611 93 74
Cuts at: 1937 2127 2189 2919 175
Size: 190 62 730 1401
Fragments arranged by size:
1401 730 611 418 261 190 151 144
93 74 62 10
BspEI T'CCGG_A
Cuts at: 194 3365 194
Size: 3171 974
BspHI T'CATG_A
Cuts at: 230 3321 230
Size: 3091 1054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 509 833 1195 1409 1711 2092 509
Size: 324 362 214 302 381 2562
Fragments arranged by size:
2562 381 362 324 302 214
BsrBI CCG'CTC
Cuts at: 50 239 2437 2491 3975 4140 50
Size: 189 2198 54 1484 165 55
Fragments arranged by size:
2198 1484 189 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2057 2057
Size: 4145
BsrFI r'CCGG_y
Cuts at: 2143 2324 2533 2143
Size: 181 209 3755
Fragments arranged by size:
3755 209 181
BssSI C'ACGA_G
Cuts at: 2416 2774 2416
Size: 358 3787
BstAPI GCAn_nnn'nTGC
Cuts at: 471 1522 471
Size: 1051 3094
BstBI TT'CG_AA
Cuts at: 2506 2506
Size: 4145
BstXI CCAn_nnnn'nTGG
Cuts at: 648 648
Size: 4145
BstYI r'GATC_y
Cuts at: 1 554 870 1170 1637 1660 1995 2241 3253
Size: 553 316 300 467 23 335 246 1012
Cuts at: 3253 3339 3351 3697 1
Size: 86 12 346 449
Fragments arranged by size:
1012 553 467 449 346 335 316 300
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 790 790
Size: 4145
BtgI C'CryG_G
Cuts at: 107 582 681 998 107
Size: 475 99 317 3254
Fragments arranged by size:
3254 475 317 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2095 2276 2439 2095
Size: 181 163 3801
Fragments arranged by size:
3801 181 163
BtsI GCAGTG_nn'
Cuts at: 297 1388 297
Size: 1091 3054
DraI TTT'AAA
Cuts at: 1144 1144
Size: 4145
DrdI GACnn_nn'nnGTC
Cuts at: 1851 2709 1851
Size: 858 3287
EaeI y'GGCC_r
Cuts at: 144 267 1730 1904 2295 2322 3667 144
Size: 123 1463 174 391 27 1345 622
Fragments arranged by size:
1463 1345 622 391 174 123 27
EagI C'GGCC_G
Cuts at: 144 1730 144
Size: 1586 2559
EarI CTCTTCn'nnn_
Cuts at: 436 604 2168 2378 436
Size: 168 1564 210 2203
Fragments arranged by size:
2203 1564 210 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2353 2663 2809 3955 2353
Size: 310 146 1146 2543
Fragments arranged by size:
2543 1146 310 146
EcoICRI GAG'CTC
Cuts at: 591 1157 591
Size: 566 3579
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 1970 2402 3149 3417 769
Size: 1201 432 747 268 1497
Fragments arranged by size:
1497 1201 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 789 1063 789
Size: 274 3871
EcoO109I rG'GnC_Cy
Cuts at: 426 1011 1284 426
Size: 585 273 3287
Fragments arranged by size:
3287 585 273
EcoRI G'AATT_C
Cuts at: 1149 1149
Size: 4145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 447 2164 447
Size: 1717 2428
FspI TGC'GCA
Cuts at: 475 1926 475
Size: 1451 2694
HaeII r_GCGC'y
Cuts at: 421 1579 1827 2849 3828 421
Size: 1158 248 1022 979 738
Fragments arranged by size:
1158 1022 979 738 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 333 365 919 951 1003 1035 1987 2017 2019
Size: 32 554 32 52 32 952 30 2
Cuts at: 2019 2049 333
Size: 30 2429
Fragments arranged by size:
2429 952 554 52 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1184 1184
Size: 4145
KasI G'GCGC_C
Cuts at: 1575 1823 1575
Size: 248 3897
KpnI G_GTAC'C
Cuts at: 1165 1165
Size: 4145
MfeI C'AATT_G
Cuts at: 326 326
Size: 4145
MluI A'CGCG_T
Cuts at: 3578 3578
Size: 4145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2816 3000 2816
Size: 184 3961
MscI TGG'CCA
Cuts at: 269 1906 269
Size: 1637 2508
MslI CAynn'nnrTG
Cuts at: 646 886 2134 2261 646
Size: 240 1248 127 2530
Fragments arranged by size:
2530 1248 240 127
MspA1I CmG'CkG
Cuts at: 259 471 584 1256 1570 1930 2943 3188 259
Size: 212 113 672 314 360 1013 245 1216
Fragments arranged by size:
1216 1013 672 360 314 245 212 113
NaeI GCC'GGC
Cuts at: 2326 2326
Size: 4145
NarI GG'CG_CC
Cuts at: 1576 1824 1576
Size: 248 3897
NcoI C'CATG_G
Cuts at: 107 681 107
Size: 574 3571
NgoMIV G'CCGG_C
Cuts at: 2324 2324
Size: 4145
NspI r_CATG'y
Cuts at: 781 855 1198 1698 2605 781
Size: 74 343 500 907 2321
Fragments arranged by size:
2321 907 500 343 74
PciI A'CATG_T
Cuts at: 2601 2601
Size: 4145
PmeI GTTT'AAAC
Cuts at: 1144 1144
Size: 4145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 4113
PpuMI rG'GwC_Cy
Cuts at: 426 1011 426
Size: 585 3560
PstI C_TGCA'G
Cuts at: 608 1192 1877 608
Size: 584 685 2876
Fragments arranged by size:
2876 685 584
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4145
PvuII CAG'CTG
Cuts at: 259 471 1570 1930 259
Size: 212 1099 360 2474
Fragments arranged by size:
2474 1099 360 212
RsrII CG'GwC_CG
Cuts at: 2340 2340
Size: 4145
SacI G_AGCT'C
Cuts at: 593 1159 593
Size: 566 3579
SacII CC_GC'GG
Cuts at: 585 585
Size: 4145
SalI G'TCGA_C
Cuts at: 1182 1182
Size: 4145
SapI GCTCTTCn'nnn_
Cuts at: 604 2168 2378 604
Size: 1564 210 2371
Fragments arranged by size:
2371 1564 210
SbfI CC_TGCA'GG
Cuts at: 1192 1192
Size: 4145
ScaI AGT'ACT
Cuts at: 3662 3662
Size: 4145
SfcI C'TryA_G
Cuts at: 33 604 1188 1873 2866 3057 33
Size: 571 584 685 993 191 1121
Fragments arranged by size:
1121 993 685 584 571 191
SfoI GGC'GCC
Cuts at: 1577 1825 1577
Size: 248 3897
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4145
SmaI CCC'GGG
Cuts at: 1167 1167
Size: 4145
SmlI C'TyrA_G
Cuts at: 421 736 1300 2215 2707 2969 421
Size: 315 564 915 492 262 1597
Fragments arranged by size:
1597 915 564 492 315 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4145
SphI G_CATG'C
Cuts at: 781 855 1198 1698 781
Size: 74 343 500 3228
Fragments arranged by size:
3228 500 343 74
StyI C'CwwG_G
Cuts at: 107 681 1081 1279 1633 107
Size: 574 400 198 354 2619
Fragments arranged by size:
2619 574 400 354 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1728 1861 2320 2529 1728
Size: 133 459 209 3344
Fragments arranged by size:
3344 459 209 133
TatI w'GTAC_w
Cuts at: 380 674 3660 380
Size: 294 2986 865
Fragments arranged by size:
2986 865 294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 862 894 3662 3694 862
Size: 32 2768 32 1313
Fragments arranged by size:
2768 1313 32 32
Tth111I GACn'n_nGTC
Cuts at: 1942 1942
Size: 4145
XbaI T'CTAG_A
Cuts at: 567 1176 567
Size: 609 3536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1640 1640
Size: 4145
XmaI C'CCGG_G
Cuts at: 1165 1165
Size: 4145
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI