Restriction map for
pF1KE0777
(Circular) (Six-base) MAPSORT of: pF1KE0777.seq Check: 2365 from: 1 to: 4106
pF1KE0777 4106 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1144 1144
Size: 4106
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 1931 2363 3110 3378 357
Size: 1574 432 747 268 1085
Fragments arranged by size:
1574 1085 747 432 268
AflIII A'CryG_T
Cuts at: 1458 2562 3539 1458
Size: 1104 977 2025
Fragments arranged by size:
2025 1104 977
AgeI A'CCGG_T
Cuts at: 2494 2494
Size: 4106
AlwNI CAG_nnn'CTG
Cuts at: 808 1411 2978 808
Size: 603 1567 1936
Fragments arranged by size:
1936 1567 603
ApaLI G'TGCA_C
Cuts at: 2876 2876
Size: 4106
ApoI r'AATT_y
Cuts at: 864 1110 3459 3715 864
Size: 246 2349 256 1255
Fragments arranged by size:
2349 1255 256 246
AseI AT'TA_AT
Cuts at: 20 20
Size: 4106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 1126 1126
Size: 4106
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4106
BanI G'GyrC_C
Cuts at: 1122 1536 1784 1819 1122
Size: 414 248 35 3409
Fragments arranged by size:
3409 414 248 35
BanII G_rGCy'C
Cuts at: 1120 2150 1120
Size: 1030 3076
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3858
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2765 4106 2004
Size: 761 1341 2004
Fragments arranged by size:
2004 1341 761
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BglII A'GATC_T
Cuts at: 368 1621 3658 368
Size: 1253 2037 816
Fragments arranged by size:
2037 1253 816
BlpI GC'TnA_GC
Cuts at: 1218 1218
Size: 4106
Bme1580I G_kGCm'C
Cuts at: 356 1731 1824 2880 356
Size: 1375 93 1056 1582
Fragments arranged by size:
1582 1375 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1318 1399 1734 3878 1318
Size: 81 335 2144 1546
Fragments arranged by size:
2144 1546 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 896 896
Size: 4106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 2161 2653 2951 1282
Size: 879 492 298 2437
Fragments arranged by size:
2437 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3810 3810
Size: 4106
BsaAI yAC'GTr
Cuts at: 1459 1459
Size: 4106
BsaBI GATnn'nnATC
Cuts at: 6 1620 3657 6
Size: 1614 2037 455
Fragments arranged by size:
2037 1614 455
BsaHI Gr'CG_yC
Cuts at: 1537 1785 2487 3983 1537
Size: 248 702 1496 1660
Fragments arranged by size:
1660 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1295 1816 2494 2768 2915 3326 1295
Size: 521 678 274 147 411 2075
Fragments arranged by size:
2075 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 426 487 1531 2289 2866 3362 426
Size: 61 1044 758 577 496 1170
Fragments arranged by size:
1170 1044 758 577 496 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 313
Size: 4106
BsiEI CG_ry'CG
Cuts at: 104 439 1648 1694 2902 104
Size: 335 1209 46 1208 1308
Fragments arranged by size:
1308 1209 1208 335 46
BsiHKAI G_wGCw'C
Cuts at: 1120 1898 2088 2880 1120
Size: 778 190 792 2346
Fragments arranged by size:
2346 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 356 1120 1731 1824 1898 2088 2150 2880 356
Size: 764 611 93 74 190 62 730 1582
Fragments arranged by size:
1582 764 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3326 3326
Size: 4106
BspHI T'CATG_A
Cuts at: 3282 3282
Size: 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1156 1370 1672 2053 1156
Size: 214 302 381 3209
Fragments arranged by size:
3209 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2398 2452 3936 4101 50
Size: 2348 54 1484 165 55
Fragments arranged by size:
2348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 692 2018 692
Size: 1326 2780
BsrFI r'CCGG_y
Cuts at: 2104 2285 2494 2104
Size: 181 209 3716
Fragments arranged by size:
3716 209 181
BssSI C'ACGA_G
Cuts at: 2377 2735 2377
Size: 358 3748
BstAPI GCAn_nnn'nTGC
Cuts at: 322 808 1483 322
Size: 486 675 2945
Fragments arranged by size:
2945 675 486
BstBI TT'CG_AA
Cuts at: 2467 2467
Size: 4106
BstYI r'GATC_y
Cuts at: 1 368 1131 1598 1621 1956 2202 3214 3300
Size: 367 763 467 23 335 246 1012 86
Cuts at: 3300 3312 3658 1
Size: 12 346 449
Fragments arranged by size:
1012 763 467 449 367 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1029 107
Size: 922 3184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2056 2237 2400 2056
Size: 181 163 3762
Fragments arranged by size:
3762 181 163
BtsI GCAGTG_nn'
Cuts at: 1050 1349 1050
Size: 299 3807
ClaI AT'CG_AT
Cuts at: 843 843
Size: 4106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 969 1004 969
Size: 35 4071
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2670 1812
Size: 858 3248
EaeI y'GGCC_r
Cuts at: 1026 1691 1865 2256 2283 3628 1026
Size: 665 174 391 27 1345 1504
Fragments arranged by size:
1504 1345 665 391 174 27
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4106
EarI CTCTTCn'nnn_
Cuts at: 462 576 862 915 2129 2339 462
Size: 114 286 53 1214 210 2229
Fragments arranged by size:
2229 1214 286 210 114 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2314 2624 2770 3916 2314
Size: 310 146 1146 2504
Fragments arranged by size:
2504 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1118 1118
Size: 4106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 896 1931 2363 3110 3378 357
Size: 539 1035 432 747 268 1085
Fragments arranged by size:
1085 1035 747 539 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1245 1245
Size: 4106
EcoRI G'AATT_C
Cuts at: 864 1110 864
Size: 246 3860
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 4106
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 1540 1788 2810 3789 1540
Size: 248 1022 979 1857
Fragments arranged by size:
1857 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1948 1978 1980 2010 1948
Size: 30 2 30 4044
Fragments arranged by size:
4044 30 30 2
HincII GTy'rAC
Cuts at: 1145 1145
Size: 4106
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3858
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 203 939 2777 2961 203
Size: 736 1838 184 1348
Fragments arranged by size:
1838 1348 736 184
MscI TGG'CCA
Cuts at: 1867 1867
Size: 4106
MslI CAynn'nnrTG
Cuts at: 602 797 2095 2222 602
Size: 195 1298 127 2486
Fragments arranged by size:
2486 1298 195 127
MspA1I CmG'CkG
Cuts at: 1217 1531 1891 2904 3149 1217
Size: 314 360 1013 245 2174
Fragments arranged by size:
2174 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4106
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3858
NcoI C'CATG_G
Cuts at: 107 107
Size: 4106
NdeI CA'TA_TG
Cuts at: 447 656 447
Size: 209 3897
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4106
NspI r_CATG'y
Cuts at: 837 1159 1659 2566 837
Size: 322 500 907 2377
Fragments arranged by size:
2377 907 500 322
PciI A'CATG_T
Cuts at: 2562 2562
Size: 4106
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4106
PfoI T'CCnGG_A
Cuts at: 873 873
Size: 4106
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 4074
PstI C_TGCA'G
Cuts at: 1153 1838 1153
Size: 685 3421
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 1531 1891 1531
Size: 360 3746
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 1120 1120
Size: 4106
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 3623 3623
Size: 4106
SfcI C'TryA_G
Cuts at: 33 1149 1834 2827 3018 33
Size: 1116 685 993 191 1121
Fragments arranged by size:
1121 1116 993 685 191
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 1261 2176 2668 2930 1261
Size: 915 492 262 2437
Fragments arranged by size:
2437 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3606
StuI AGG'CCT
Cuts at: 286 286
Size: 4106
StyI C'CwwG_G
Cuts at: 107 1240 1594 107
Size: 1133 354 2619
Fragments arranged by size:
2619 1133 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1006 1689 1822 2281 2490 1006
Size: 683 133 459 209 2622
Fragments arranged by size:
2622 683 459 209 133
TatI w'GTAC_w
Cuts at: 3621 3621
Size: 4106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3623 3655 3623
Size: 32 4074
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1601 1601
Size: 4106
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
XmnI GAAnn'nnTTC
Cuts at: 203 203
Size: 4106
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI