Restriction map for
pF1KE0778
(Circular) (Six-base) MAPSORT of: pF1KE0778.seq Check: 3268 from: 1 to: 4655
pF1KE0778 4655 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1453 1693 1453
Size: 240 4415
Acc65I G'GTAC_C
Cuts at: 1671 1671
Size: 4655
AclI AA'CG_TT
Cuts at: 4495 4495
Size: 4655
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 1391 1412 2480 2912 3659 3927 1004
Size: 387 21 1068 432 747 268 1732
Fragments arranged by size:
1732 1068 747 432 387 268 21
AflIII A'CryG_T
Cuts at: 955 1049 2007 3111 4088 955
Size: 94 958 1104 977 1522
Fragments arranged by size:
1522 1104 977 958 94
AgeI A'CCGG_T
Cuts at: 3043 3043
Size: 4655
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1493 1493
Size: 4655
AlwNI CAG_nnn'CTG
Cuts at: 1960 3527 1960
Size: 1567 3088
ApaLI G'TGCA_C
Cuts at: 3425 3425
Size: 4655
ApoI r'AATT_y
Cuts at: 713 1149 1659 4008 4264 713
Size: 436 510 2349 256 1104
Fragments arranged by size:
2349 1104 510 436 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4655
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
AvaI C'yCGr_G
Cuts at: 1675 1675
Size: 4655
BamHI G'GATC_C
Cuts at: 1680 1680
Size: 4655
BanI G'GyrC_C
Cuts at: 1621 1671 2085 2333 2368 1621
Size: 50 414 248 35 3908
Fragments arranged by size:
3908 414 248 50 35
BanII G_rGCy'C
Cuts at: 1669 2699 1669
Size: 1030 3625
BbeI G_GCGC'C
Cuts at: 1625 2089 2337 1625
Size: 464 248 3943
Fragments arranged by size:
3943 464 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1020 1054 1020
Size: 34 4621
BciVI GTATCCnnnnn_n'
Cuts at: 2553 3314 4655 2553
Size: 761 1341 2553
Fragments arranged by size:
2553 1341 761
BclI T'GATC_A
Cuts at: 2175 2175
Size: 4655
BglII A'GATC_T
Cuts at: 819 2170 4207 819
Size: 1351 2037 1267
Fragments arranged by size:
2037 1351 1267
BlpI GC'TnA_GC
Cuts at: 909 1767 909
Size: 858 3797
Bme1580I G_kGCm'C
Cuts at: 999 2280 2373 3429 999
Size: 1281 93 1056 2225
Fragments arranged by size:
2225 1281 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1867 1948 2283 4427 1867
Size: 81 335 2144 2095
Fragments arranged by size:
2144 2095 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 4655
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 298 303 197
Size: 101 5 4549
Fragments arranged by size:
4549 101 5
Bpu10I CC'TnA_GC
Cuts at: 1223 1223
Size: 4655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 1831 2710 3202 3500 321
Size: 1510 879 492 298 1476
Fragments arranged by size:
1510 1476 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1269 4359 1269
Size: 3090 1565
BsaAI yAC'GTr
Cuts at: 1060 2008 1060
Size: 948 3707
BsaBI GATnn'nnATC
Cuts at: 6 2169 4206 6
Size: 2163 2037 455
Fragments arranged by size:
2163 2037 455
BsaHI Gr'CG_yC
Cuts at: 1037 1622 2086 2334 3036 4532 1037
Size: 585 464 248 702 1496 1160
Fragments arranged by size:
1496 1160 702 585 464 248
BsaWI w'CCGG_w
Cuts at: 1844 2365 3043 3317 3464 3875 1844
Size: 521 678 274 147 411 2624
Fragments arranged by size:
2624 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1453 1453
Size: 4655
BseYI C'CCAG_C
Cuts at: 2080 2838 3415 3911 2080
Size: 758 577 496 2824
Fragments arranged by size:
2824 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 1527 278
Size: 1249 3406
BsiEI CG_ry'CG
Cuts at: 104 2197 2243 3451 104
Size: 2093 46 1208 1308
Fragments arranged by size:
2093 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1669 2447 2637 3429 1669
Size: 778 190 792 2895
Fragments arranged by size:
2895 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 999 1669 2280 2373 2447 2637 2699 3429 999
Size: 670 611 93 74 190 62 730 2225
Fragments arranged by size:
2225 730 670 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3875 3875
Size: 4655
BspHI T'CATG_A
Cuts at: 3831 3831
Size: 4655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1705 1919 2221 2602 1705
Size: 214 302 381 3758
Fragments arranged by size:
3758 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2947 3001 4485 4650 50
Size: 2897 54 1484 165 55
Fragments arranged by size:
2897 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2567 2567
Size: 4655
BsrFI r'CCGG_y
Cuts at: 2653 2834 3043 2653
Size: 181 209 4265
Fragments arranged by size:
4265 209 181
BsrGI T'GTAC_A
Cuts at: 810 1046 810
Size: 236 4419
BssSI C'ACGA_G
Cuts at: 2926 3284 2926
Size: 358 4297
BstAPI GCAn_nnn'nTGC
Cuts at: 2032 2032
Size: 4655
BstBI TT'CG_AA
Cuts at: 3016 3016
Size: 4655
BstYI r'GATC_y
Cuts at: 1 819 1200 1680 2147 2170 2505 2751 3763
Size: 818 381 480 467 23 335 246 1012
Cuts at: 3763 3849 3861 4207 1
Size: 86 12 346 449
Fragments arranged by size:
1012 818 480 467 449 381 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4655
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2605 2786 2949 2605
Size: 181 163 4311
Fragments arranged by size:
4311 181 163
BtsI GCAGTG_nn'
Cuts at: 1021 1898 1021
Size: 877 3778
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1058 1093 1058
Size: 35 4620
DraI TTT'AAA
Cuts at: 1654 1654
Size: 4655
DrdI GACnn_nn'nnGTC
Cuts at: 2361 3219 2361
Size: 858 3797
EaeI y'GGCC_r
Cuts at: 2240 2414 2805 2832 4177 2240
Size: 174 391 27 1345 2718
Fragments arranged by size:
2718 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2240 2240
Size: 4655
EarI CTCTTCn'nnn_
Cuts at: 1085 1343 1430 2678 2888 1085
Size: 258 87 1248 210 2852
Fragments arranged by size:
2852 1248 258 210 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2863 3173 3319 4465 2863
Size: 310 146 1146 3053
Fragments arranged by size:
3053 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1667 1667
Size: 4655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 298 303 1004 1391 1412 2480 2912 3659
Size: 101 5 701 387 21 1068 432 747
Cuts at: 3659 3927 197
Size: 268 925
Fragments arranged by size:
1068 925 747 701 432 387 268 101
21 5
EcoO109I rG'GnC_Cy
Cuts at: 1794 1794
Size: 4655
EcoRI G'AATT_C
Cuts at: 1149 1659 1149
Size: 510 4145
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 196 1114 1512 2674 196
Size: 918 398 1162 2177
Fragments arranged by size:
2177 1162 918 398
FspI TGC'GCA
Cuts at: 2436 2436
Size: 4655
HaeII r_GCGC'y
Cuts at: 1625 2089 2337 3359 4338 1625
Size: 464 248 1022 979 1942
Fragments arranged by size:
1942 1022 979 464 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 142 150 174 301 319 333 351 2497
Size: 24 8 24 127 18 14 18 2146
Cuts at: 2497 2527 2529 2559 118
Size: 30 2 30 2214
Fragments arranged by size:
2214 2146 127 30 30 24 24 18
18 14 8 2
HincII GTy'rAC
Cuts at: 1250 1694 1250
Size: 444 4211
HindIII A'AGCT_T
Cuts at: 1096 1096
Size: 4655
HpaI GTT'AAC
Cuts at: 1250 1250
Size: 4655
KasI G'GCGC_C
Cuts at: 1621 2085 2333 1621
Size: 464 248 3943
Fragments arranged by size:
3943 464 248
KpnI G_GTAC'C
Cuts at: 1675 1675
Size: 4655
MluI A'CGCG_T
Cuts at: 4088 4088
Size: 4655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3326 3510 3326
Size: 184 4471
MscI TGG'CCA
Cuts at: 2416 2416
Size: 4655
MslI CAynn'nnrTG
Cuts at: 800 892 2644 2771 800
Size: 92 1752 127 2684
Fragments arranged by size:
2684 1752 127 92
MspA1I CmG'CkG
Cuts at: 1543 1766 2080 2440 3453 3698 1543
Size: 223 314 360 1013 245 2500
Fragments arranged by size:
2500 1013 360 314 245 223
NaeI GCC'GGC
Cuts at: 2836 2836
Size: 4655
NarI GG'CG_CC
Cuts at: 1622 2086 2334 1622
Size: 464 248 3943
Fragments arranged by size:
3943 464 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4655
NgoMIV G'CCGG_C
Cuts at: 2834 2834
Size: 4655
NspI r_CATG'y
Cuts at: 959 1053 1708 2208 3115 959
Size: 94 655 500 907 2499
Fragments arranged by size:
2499 907 655 500 94
PasI CC'CwG_GG
Cuts at: 1397 1397
Size: 4655
PciI A'CATG_T
Cuts at: 955 1049 3111 955
Size: 94 2062 2499
Fragments arranged by size:
2499 2062 94
PfoI T'CCnGG_A
Cuts at: 319 319
Size: 4655
PmeI GTTT'AAAC
Cuts at: 1654 1654
Size: 4655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 3794
Size: 32 4623
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 617 649 617
Size: 32 4623
PstI C_TGCA'G
Cuts at: 950 1702 2387 950
Size: 752 685 3218
Fragments arranged by size:
3218 752 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4655
PvuII CAG'CTG
Cuts at: 1543 2080 2440 1543
Size: 537 360 3758
Fragments arranged by size:
3758 537 360
RsrII CG'GwC_CG
Cuts at: 2850 2850
Size: 4655
SacI G_AGCT'C
Cuts at: 1669 1669
Size: 4655
SalI G'TCGA_C
Cuts at: 1692 1692
Size: 4655
SapI GCTCTTCn'nnn_
Cuts at: 2678 2888 2678
Size: 210 4445
SbfI CC_TGCA'GG
Cuts at: 1702 1702
Size: 4655
ScaI AGT'ACT
Cuts at: 4172 4172
Size: 4655
SfcI C'TryA_G
Cuts at: 33 782 946 1005 1698 2383 3376 3567 33
Size: 749 164 59 693 685 993 191 1121
Fragments arranged by size:
1121 993 749 693 685 191 164 59
SfoI GGC'GCC
Cuts at: 1623 2087 2335 1623
Size: 464 248 3943
Fragments arranged by size:
3943 464 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
SmaI CCC'GGG
Cuts at: 1677 1677
Size: 4655
SmlI C'TyrA_G
Cuts at: 336 1810 2725 3217 3479 336
Size: 1474 915 492 262 1512
Fragments arranged by size:
1512 1474 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4655
SphI G_CATG'C
Cuts at: 1708 2208 1708
Size: 500 4155
SspI AAT'ATT
Cuts at: 470 470
Size: 4655
StyI C'CwwG_G
Cuts at: 107 1587 1789 2143 107
Size: 1480 202 354 2619
Fragments arranged by size:
2619 1480 354 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2238 2371 2830 3039 2238
Size: 133 459 209 3854
Fragments arranged by size:
3854 459 209 133
TatI w'GTAC_w
Cuts at: 177 810 1046 4170 177
Size: 633 236 3124 662
Fragments arranged by size:
3124 662 633 236
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4172 4204 4172
Size: 32 4623
Tth111I GACn'n_nGTC
Cuts at: 2452 2452
Size: 4655
XbaI T'CTAG_A
Cuts at: 1686 1686
Size: 4655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2150 2150
Size: 4655
XmaI C'CCGG_G
Cuts at: 1675 1675
Size: 4655
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI