Restriction map for
pF1KE0781
(Circular) (Six-base) MAPSORT of: pF1KE0781.seq Check: 5210 from: 1 to: 4163
pF1KE0781 4163 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 986 1988 2420 3167 3435 883
Size: 103 1002 432 747 268 1611
Fragments arranged by size:
1611 1002 747 432 268 103
AflIII A'CryG_T
Cuts at: 692 1515 2619 3596 692
Size: 823 1104 977 1259
Fragments arranged by size:
1259 1104 977 823
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AlwNI CAG_nnn'CTG
Cuts at: 1468 3035 1468
Size: 1567 2596
ApaLI G'TGCA_C
Cuts at: 1075 2933 1075
Size: 1858 2305
ApoI r'AATT_y
Cuts at: 208 430 792 826 1167 3516 3772 208
Size: 222 362 34 341 2349 256 599
Fragments arranged by size:
2349 599 362 341 256 222 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 1183 1183
Size: 4163
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 487 1179 1593 1841 1876 487
Size: 692 414 248 35 2774
Fragments arranged by size:
2774 692 414 248 35
BanII G_rGCy'C
Cuts at: 1177 2207 1177
Size: 1030 3133
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BbsI GAAGACnn'nnnn_
Cuts at: 923 923
Size: 4163
BciVI GTATCCnnnnn_n'
Cuts at: 615 2061 2822 4163 615
Size: 1446 761 1341 615
Fragments arranged by size:
1446 1341 761 615
BclI T'GATC_A
Cuts at: 1683 1683
Size: 4163
BfrBI ATG'CAT
Cuts at: 413 413
Size: 4163
BglI GCCn_nnn'nGGC
Cuts at: 635 635
Size: 4163
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 1275 1275
Size: 4163
Bme1580I G_kGCm'C
Cuts at: 1079 1788 1881 2937 1079
Size: 709 93 1056 2305
Fragments arranged by size:
2305 1056 709 93
BmrI ACTGGGnnnn_n'
Cuts at: 1160 1375 1456 1791 3935 1160
Size: 215 81 335 2144 1388
Fragments arranged by size:
2144 1388 335 215 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1032 2530 1032
Size: 1498 2665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 707 385
Size: 322 3841
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 930 1002 1339 2218 2710 3008 930
Size: 72 337 879 492 298 2085
Fragments arranged by size:
2085 879 492 337 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 167 1516 167
Size: 1349 2814
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 1594 1842 2544 4040 1594
Size: 248 702 1496 1717
Fragments arranged by size:
1717 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2825 2972 3383 1352
Size: 521 678 274 147 411 2132
Fragments arranged by size:
2132 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 382
Size: 4163
BseYI C'CCAG_C
Cuts at: 949 1588 2346 2923 3419 949
Size: 639 758 577 496 1693
Fragments arranged by size:
1693 758 639 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 916 725
Size: 191 3972
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 268 1079 1177 1955 2145 2937 268
Size: 811 98 778 190 792 1494
Fragments arranged by size:
1494 811 792 778 190 98
Bsp1286I G_dGCh'C
Cuts at: 268 1079 1177 1788 1881 1955 2145 2207 2937
Size: 811 98 611 93 74 190 62 730
Cuts at: 2937 268
Size: 1494
Fragments arranged by size:
1494 811 730 611 190 98 93 74
62
BspEI T'CCGG_A
Cuts at: 3383 3383
Size: 4163
BspHI T'CATG_A
Cuts at: 641 3339 641
Size: 2698 1465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 938 1213 1427 1729 2110 938
Size: 275 214 302 381 2991
Fragments arranged by size:
2991 381 302 275 214
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2075 2075
Size: 4163
BsrFI r'CCGG_y
Cuts at: 2161 2342 2551 2161
Size: 181 209 3773
Fragments arranged by size:
3773 209 181
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 1540 1540
Size: 4163
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstXI CCAn_nnnn'nTGG
Cuts at: 1137 1137
Size: 4163
BstYI r'GATC_y
Cuts at: 1 960 1188 1655 1678 2013 2259 3271 3357
Size: 959 228 467 23 335 246 1012 86
Cuts at: 3357 3369 3715 1
Size: 12 346 449
Fragments arranged by size:
1012 959 467 449 346 335 246 228
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 638 2113 2294 2457 638
Size: 1475 181 163 2344
Fragments arranged by size:
2344 1475 181 163
BtsI GCAGTG_nn'
Cuts at: 567 713 1406 567
Size: 146 693 3324
Fragments arranged by size:
3324 693 146
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DraIII CAC_nnn'GTG
Cuts at: 1118 1118
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 1748 1922 2313 2340 3685 1748
Size: 174 391 27 1345 2226
Fragments arranged by size:
2226 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 782 1024 1034 2186 2396 782
Size: 242 10 1152 210 2549
Fragments arranged by size:
2549 1152 242 210 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 2681 2827 3973 2371
Size: 310 146 1146 2561
Fragments arranged by size:
2561 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 707 883 986 1988 2420 3167 3435 385
Size: 322 176 103 1002 432 747 268 1113
Fragments arranged by size:
1113 1002 747 432 322 268 176 103
EcoO109I rG'GnC_Cy
Cuts at: 1302 1302
Size: 4163
EcoRI G'AATT_C
Cuts at: 208 792 826 1167 208
Size: 584 34 341 3204
Fragments arranged by size:
3204 584 341 34
EcoRV GAT'ATC
Cuts at: 393 393
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 924 1063 2182 924
Size: 139 1119 2905
Fragments arranged by size:
2905 1119 139
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 1597 1845 2867 3846 1597
Size: 248 1022 979 1914
Fragments arranged by size:
1914 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 549 581 2005 2035 2037 2067 549
Size: 32 1424 30 2 30 2645
Fragments arranged by size:
2645 1424 32 30 30 2
HincII GTy'rAC
Cuts at: 1202 1202
Size: 4163
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 632 2834 3018 632
Size: 2202 184 1777
Fragments arranged by size:
2202 1777 184
MscI TGG'CCA
Cuts at: 1924 1924
Size: 4163
MslI CAynn'nnrTG
Cuts at: 938 1135 2152 2279 938
Size: 197 1017 127 2822
Fragments arranged by size:
2822 1017 197 127
MspA1I CmG'CkG
Cuts at: 255 1274 1588 1948 2961 3206 255
Size: 1019 314 360 1013 245 1212
Fragments arranged by size:
1212 1019 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NcoI C'CATG_G
Cuts at: 107 107
Size: 4163
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NsiI A_TGCA'T
Cuts at: 415 415
Size: 4163
NspI r_CATG'y
Cuts at: 696 1216 1716 2623 696
Size: 520 500 907 2236
Fragments arranged by size:
2236 907 520 500
PasI CC'CwG_GG
Cuts at: 966 966
Size: 4163
PciI A'CATG_T
Cuts at: 692 2619 692
Size: 1927 2236
PflMI CCAn_nnn'nTGG
Cuts at: 772 772
Size: 4163
PfoI T'CCnGG_A
Cuts at: 362 734 362
Size: 372 3791
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PstI C_TGCA'G
Cuts at: 680 1210 1895 680
Size: 530 685 2948
Fragments arranged by size:
2948 685 530
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 1588 1948 1588
Size: 360 3803
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 676 1206 1891 2884 3075 33
Size: 643 530 685 993 191 1121
Fragments arranged by size:
1121 993 685 643 530 191
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 909 981 1318 2233 2725 2987 909
Size: 72 337 915 492 262 2085
Fragments arranged by size:
2085 915 492 337 262 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
StuI AGG'CCT
Cuts at: 638 638
Size: 4163
StyI C'CwwG_G
Cuts at: 107 1103 1297 1651 107
Size: 996 194 354 2619
Fragments arranged by size:
2619 996 354 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 1879 2338 2547 1746
Size: 133 459 209 3362
Fragments arranged by size:
3362 459 209 133
TatI w'GTAC_w
Cuts at: 3678 3678
Size: 4163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4131
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 540 1194 540
Size: 654 3509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 705 1658 705
Size: 953 3210
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI