Restriction map for
pF1KE0782
(Circular) (Six-base) MAPSORT of: pF1KE0782.seq Check: 8924 from: 1 to: 4472
pF1KE0782 4472 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1510 1510
Size: 4472
Acc65I G'GTAC_C
Cuts at: 1488 1488
Size: 4472
AclI AA'CG_TT
Cuts at: 4312 4312
Size: 4472
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1243 1425 2297 2729 3476 3744 1243
Size: 182 872 432 747 268 1971
Fragments arranged by size:
1971 872 747 432 268 182
AfeI AGC'GCT
Cuts at: 122 1171 122
Size: 1049 3423
AflIII A'CryG_T
Cuts at: 197 404 1824 2928 3905 197
Size: 207 1420 1104 977 764
Fragments arranged by size:
1420 1104 977 764 207
AgeI A'CCGG_T
Cuts at: 2860 2860
Size: 4472
AhdI GACnn_n'nnGTC
Cuts at: 780 780
Size: 4472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 413 1204 1295 413
Size: 791 91 3590
Fragments arranged by size:
3590 791 91
AlwNI CAG_nnn'CTG
Cuts at: 1777 3344 1777
Size: 1567 2905
ApaLI G'TGCA_C
Cuts at: 3242 3242
Size: 4472
ApoI r'AATT_y
Cuts at: 1262 1287 1476 3825 4081 1262
Size: 25 189 2349 256 1653
Fragments arranged by size:
2349 1653 256 189 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 4472
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
AvaI C'yCGr_G
Cuts at: 158 705 1362 1492 158
Size: 547 657 130 3138
Fragments arranged by size:
3138 657 547 130
BamHI G'GATC_C
Cuts at: 1497 1497
Size: 4472
BanI G'GyrC_C
Cuts at: 174 379 478 1488 1902 2150 2185 174
Size: 205 99 1010 414 248 35 2461
Fragments arranged by size:
2461 1010 414 248 205 99 35
BanII G_rGCy'C
Cuts at: 167 290 584 1486 2516 167
Size: 123 294 902 1030 2123
Fragments arranged by size:
2123 1030 902 294 123
BbeI G_GCGC'C
Cuts at: 178 482 1906 2154 178
Size: 304 1424 248 2496
Fragments arranged by size:
2496 1424 304 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 876 910 1353 1387 90
Size: 34 752 34 443 34 3175
Fragments arranged by size:
3175 752 443 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2370 3131 4472 2370
Size: 761 1341 2370
Fragments arranged by size:
2370 1341 761
BclI T'GATC_A
Cuts at: 662 1992 662
Size: 1330 3142
BglI GCCn_nnn'nGGC
Cuts at: 112 139 148 230 112
Size: 27 9 82 4354
Fragments arranged by size:
4354 82 27 9
BglII A'GATC_T
Cuts at: 1987 4024 1987
Size: 2037 2435
BlpI GC'TnA_GC
Cuts at: 505 1584 505
Size: 1079 3393
Bme1580I G_kGCm'C
Cuts at: 242 392 620 2097 2190 3246 242
Size: 150 228 1477 93 1056 1468
Fragments arranged by size:
1477 1468 1056 228 150 93
BmgBI CAC'GTC
Cuts at: 198 543 198
Size: 345 4127
BmrI ACTGGGnnnn_n'
Cuts at: 1684 1765 2100 4244 1684
Size: 81 335 2144 1912
Fragments arranged by size:
2144 1912 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 142 2839 142
Size: 2697 1775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1429 349
Size: 1080 3392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1057 1648 2527 3019 3317 706
Size: 351 591 879 492 298 1861
Fragments arranged by size:
1861 879 591 492 351 298
BsaI GGTCTCn'nnnn_
Cuts at: 499 4176 499
Size: 3677 795
BsaAI yAC'GTr
Cuts at: 237 681 1825 237
Size: 444 1144 2884
Fragments arranged by size:
2884 1144 444
BsaBI GATnn'nnATC
Cuts at: 6 1986 4023 6
Size: 1980 2037 455
Fragments arranged by size:
2037 1980 455
BsaHI Gr'CG_yC
Cuts at: 175 454 479 1347 1903 2151 2853 4349 175
Size: 279 25 868 556 248 702 1496 298
Fragments arranged by size:
1496 868 702 556 298 279 248 25
BsaWI w'CCGG_w
Cuts at: 1661 2182 2860 3134 3281 3692 1661
Size: 521 678 274 147 411 2441
Fragments arranged by size:
2441 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1003 1033 1380 1410 1003
Size: 30 347 30 4065
Fragments arranged by size:
4065 347 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1388 1426 1388
Size: 38 4434
BseYI C'CCAG_C
Cuts at: 211 837 938 957 1897 2655 3232 3728 211
Size: 626 101 19 940 758 577 496 955
Fragments arranged by size:
955 940 758 626 577 496 101 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 748
Size: 4472
BsiEI CG_ry'CG
Cuts at: 104 851 2014 2060 3268 104
Size: 747 1163 46 1208 1308
Fragments arranged by size:
1308 1208 1163 747 46
BsiHKAI G_wGCw'C
Cuts at: 584 1486 2264 2454 3246 584
Size: 902 778 190 792 1810
Fragments arranged by size:
1810 902 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 167 242 290 392 584 620 1486 2097 2190
Size: 75 48 102 192 36 866 611 93
Cuts at: 2190 2264 2454 2516 3246 167
Size: 74 190 62 730 1393
Fragments arranged by size:
1393 866 730 611 192 190 102 93
75 74 62 48 36
BspEI T'CCGG_A
Cuts at: 3692 3692
Size: 4472
BspHI T'CATG_A
Cuts at: 3648 3648
Size: 4472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 863 1522 1736 2038 2419 863
Size: 659 214 302 381 2916
Fragments arranged by size:
2916 659 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2764 2818 4302 4467 50
Size: 2714 54 1484 165 55
Fragments arranged by size:
2714 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 533 788 2384 533
Size: 255 1596 2621
Fragments arranged by size:
2621 1596 255
BsrFI r'CCGG_y
Cuts at: 655 2470 2651 2860 655
Size: 1815 181 209 2267
Fragments arranged by size:
2267 1815 209 181
BssHII G'CGCG_C
Cuts at: 187 410 434 187
Size: 223 24 4225
Fragments arranged by size:
4225 223 24
BssSI C'ACGA_G
Cuts at: 2743 3101 2743
Size: 358 4114
BstAPI GCAn_nnn'nTGC
Cuts at: 257 1849 257
Size: 1592 2880
BstBI TT'CG_AA
Cuts at: 2833 2833
Size: 4472
BstYI r'GATC_y
Cuts at: 1 803 1497 1964 1987 2322 2568 3580 3666
Size: 802 694 467 23 335 246 1012 86
Cuts at: 3666 3678 4024 1
Size: 12 346 449
Fragments arranged by size:
1012 802 694 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1454 1454
Size: 4472
BtgI C'CryG_G
Cuts at: 566 566
Size: 4472
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1431 2422 2603 2766 1431
Size: 991 181 163 3137
Fragments arranged by size:
3137 991 181 163
BtsI GCAGTG_nn'
Cuts at: 932 1263 1715 932
Size: 331 452 3689
Fragments arranged by size:
3689 452 331
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1193 1228 1193
Size: 35 4437
DraI TTT'AAA
Cuts at: 1471 1471
Size: 4472
DraIII CAC_nnn'GTG
Cuts at: 372 684 372
Size: 312 4160
DrdI GACnn_nn'nnGTC
Cuts at: 1330 2178 3036 1330
Size: 848 858 2766
Fragments arranged by size:
2766 858 848
EaeI y'GGCC_r
Cuts at: 131 140 422 606 848 878 2057 2231 2622
Size: 9 282 184 242 30 1179 174 391
Cuts at: 2622 2649 3994 131
Size: 27 1345 609
Fragments arranged by size:
1345 1179 609 391 282 242 184 174
30 27 9
EagI C'GGCC_G
Cuts at: 848 2057 848
Size: 1209 3263
EarI CTCTTCn'nnn_
Cuts at: 589 1444 2495 2705 589
Size: 855 1051 210 2356
Fragments arranged by size:
2356 1051 855 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2680 2990 3136 4282 2680
Size: 310 146 1146 2870
Fragments arranged by size:
2870 1146 310 146
EcoICRI GAG'CTC
Cuts at: 582 1484 582
Size: 902 3570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1243 1425 1429 2297 2729 3476 3744 349
Size: 894 182 4 868 432 747 268 1077
Fragments arranged by size:
1077 894 868 747 432 268 182 4
EcoNI CCTnn'n_nnAGG
Cuts at: 1408 1408
Size: 4472
EcoO109I rG'GnC_Cy
Cuts at: 1611 1611
Size: 4472
EcoRI G'AATT_C
Cuts at: 1287 1476 1287
Size: 189 4283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 546 1188 2491 546
Size: 642 1303 2527
Fragments arranged by size:
2527 1303 642
FseI GG_CCGG'CC
Cuts at: 659 659
Size: 4472
FspI TGC'GCA
Cuts at: 2253 2253
Size: 4472
HaeII r_GCGC'y
Cuts at: 124 154 178 482 1173 1906 2154 3176 4155
Size: 30 24 304 691 733 248 1022 979
Cuts at: 4155 124
Size: 441
Fragments arranged by size:
1022 979 733 691 441 304 248 30
24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 766 798 1295 1327 1380 1412 2314 2344 2346
Size: 32 497 32 53 32 902 30 2
Cuts at: 2346 2376 766
Size: 30 2862
Fragments arranged by size:
2862 902 497 53 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1341 1511 1341
Size: 170 4302
KasI G'GCGC_C
Cuts at: 174 478 1902 2150 174
Size: 304 1424 248 2496
Fragments arranged by size:
2496 1424 304 248
KpnI G_GTAC'C
Cuts at: 1492 1492
Size: 4472
MluI A'CGCG_T
Cuts at: 3905 3905
Size: 4472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 764 998 3143 3327 764
Size: 234 2145 184 1909
Fragments arranged by size:
2145 1909 234 184
MscI TGG'CCA
Cuts at: 880 2233 880
Size: 1353 3119
MslI CAynn'nnrTG
Cuts at: 395 1186 2461 2588 395
Size: 791 1275 127 2279
Fragments arranged by size:
2279 1275 791 127
MspA1I CmG'CkG
Cuts at: 221 353 359 468 504 600 629 942 1583
Size: 132 6 109 36 96 29 313 641
Cuts at: 1583 1897 2257 3270 3515 221
Size: 314 360 1013 245 1178
Fragments arranged by size:
1178 1013 641 360 314 313 245 132
109 96 36 29 6
NaeI GCC'GGC
Cuts at: 657 2653 657
Size: 1996 2476
NarI GG'CG_CC
Cuts at: 175 479 1903 2151 175
Size: 304 1424 248 2496
Fragments arranged by size:
2496 1424 304 248
NgoMIV G'CCGG_C
Cuts at: 655 2651 655
Size: 1996 2476
NspI r_CATG'y
Cuts at: 1525 2025 2932 1525
Size: 500 907 3065
Fragments arranged by size:
3065 907 500
PciI A'CATG_T
Cuts at: 2928 2928
Size: 4472
PfoI T'CCnGG_A
Cuts at: 448 965 448
Size: 517 3955
PmeI GTTT'AAAC
Cuts at: 1471 1471
Size: 4472
PmlI CAC'GTG
Cuts at: 681 681
Size: 4472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4440
PspXI vC'TCGA_Gb
Cuts at: 1362 1362
Size: 4472
PstI C_TGCA'G
Cuts at: 419 443 473 1376 1519 2204 419
Size: 24 30 903 143 685 2687
Fragments arranged by size:
2687 903 685 143 30 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4472
PvuII CAG'CTG
Cuts at: 468 504 1897 2257 468
Size: 36 1393 360 2683
Fragments arranged by size:
2683 1393 360 36
RsrII CG'GwC_CG
Cuts at: 2667 2667
Size: 4472
SacI G_AGCT'C
Cuts at: 584 1486 584
Size: 902 3570
SalI G'TCGA_C
Cuts at: 1509 1509
Size: 4472
SapI GCTCTTCn'nnn_
Cuts at: 589 2495 2705 589
Size: 1906 210 2356
Fragments arranged by size:
2356 1906 210
SbfI CC_TGCA'GG
Cuts at: 1519 1519
Size: 4472
ScaI AGT'ACT
Cuts at: 3989 3989
Size: 4472
SfcI C'TryA_G
Cuts at: 33 415 439 469 733 901 1372 1515 2200
Size: 382 24 30 264 168 471 143 685
Cuts at: 2200 3193 3384 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 471 382 264 191 168
143 30 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 139 139
Size: 4472
SfoI GGC'GCC
Cuts at: 176 480 1904 2152 176
Size: 304 1424 248 2496
Fragments arranged by size:
2496 1424 304 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
SmaI CCC'GGG
Cuts at: 160 707 1494 160
Size: 547 787 3138
Fragments arranged by size:
3138 787 547
SmlI C'TyrA_G
Cuts at: 721 1072 1362 1627 2542 3034 3296 721
Size: 351 290 265 915 492 262 1897
Fragments arranged by size:
1897 915 492 351 290 265 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4472
SphI G_CATG'C
Cuts at: 1525 2025 1525
Size: 500 3972
StyI C'CwwG_G
Cuts at: 1606 1960 1606
Size: 354 4118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1405 2055 2188 2647 2856 1405
Size: 650 133 459 209 3021
Fragments arranged by size:
3021 650 459 209 133
TatI w'GTAC_w
Cuts at: 3987 3987
Size: 4472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 670 702 1019 1051 1253 1285 3989 4021 670
Size: 32 317 32 202 32 2704 32 1121
Fragments arranged by size:
2704 1121 317 202 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1392 2269 1392
Size: 877 3595
XbaI T'CTAG_A
Cuts at: 1503 1503
Size: 4472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 678 1967 678
Size: 1289 3183
XhoI C'TCGA_G
Cuts at: 1362 1362
Size: 4472
XmaI C'CCGG_G
Cuts at: 158 705 1492 158
Size: 547 787 3138
Fragments arranged by size:
3138 787 547
XmnI GAAnn'nnTTC
Cuts at: 1171 1171
Size: 4472
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PmlI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I ClaI EcoRV FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
StuI SwaI ZraI