Restriction map for
pF1KE0786
(Circular) (Six-base) MAPSORT of: pF1KE0786.seq Check: 9442 from: 1 to: 4484
pF1KE0786 4484 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1387 1387
Size: 4484
AccI GT'mk_AC
Cuts at: 1522 1522
Size: 4484
Acc65I G'GTAC_C
Cuts at: 1500 1500
Size: 4484
AclI AA'CG_TT
Cuts at: 4324 4324
Size: 4484
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1195 1347 2309 2741 3488 3756 688
Size: 507 152 962 432 747 268 1416
Fragments arranged by size:
1416 962 747 507 432 268 152
AfeI AGC'GCT
Cuts at: 805 805
Size: 4484
AflIII A'CryG_T
Cuts at: 1004 1836 2940 3917 1004
Size: 832 1104 977 1571
Fragments arranged by size:
1571 1104 977 832
AgeI A'CCGG_T
Cuts at: 2872 2872
Size: 4484
AhdI GACnn_n'nnGTC
Cuts at: 523 523
Size: 4484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 917 917
Size: 4484
AlwNI CAG_nnn'CTG
Cuts at: 549 1789 3356 549
Size: 1240 1567 1677
Fragments arranged by size:
1677 1567 1240
ApaI G_GGCC'C
Cuts at: 360 360
Size: 4484
ApaLI G'TGCA_C
Cuts at: 1192 3254 1192
Size: 2062 2422
ApoI r'AATT_y
Cuts at: 1488 3837 4093 1488
Size: 2349 256 1879
Fragments arranged by size:
2349 1879 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4484
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4484
AvaI C'yCGr_G
Cuts at: 1504 1504
Size: 4484
BamHI G'GATC_C
Cuts at: 1509 1509
Size: 4484
BanI G'GyrC_C
Cuts at: 468 631 684 912 1081 1500 1914 2162 2197
Size: 163 53 228 169 419 414 248 35
Cuts at: 2197 468
Size: 2755
Fragments arranged by size:
2755 419 414 248 228 169 163 53
35
BanII G_rGCy'C
Cuts at: 360 734 1498 2528 360
Size: 374 764 1030 2316
Fragments arranged by size:
2316 1030 764 374
BbeI G_GCGC'C
Cuts at: 472 1085 1918 2166 472
Size: 613 833 248 2790
Fragments arranged by size:
2790 833 613 248
BbvCI CC'TCA_GC
Cuts at: 337 349 337
Size: 12 4472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 790 813 824 847 790
Size: 23 11 23 4427
Fragments arranged by size:
4427 23 23 11
BciVI GTATCCnnnnn_n'
Cuts at: 2382 3143 4484 2382
Size: 761 1341 2382
Fragments arranged by size:
2382 1341 761
BclI T'GATC_A
Cuts at: 2004 2004
Size: 4484
BglI GCCn_nnn'nGGC
Cuts at: 238 238
Size: 4484
BglII A'GATC_T
Cuts at: 1999 4036 1999
Size: 2037 2447
BlpI GC'TnA_GC
Cuts at: 259 1596 259
Size: 1337 3147
Bme1580I G_kGCm'C
Cuts at: 188 360 689 1196 1265 2109 2202 3258 188
Size: 172 329 507 69 844 93 1056 1414
Fragments arranged by size:
1414 1056 844 507 329 172 93 69
BmgBI CAC'GTC
Cuts at: 1110 1110
Size: 4484
BmrI ACTGGGnnnn_n'
Cuts at: 261 1696 1777 2112 4256 261
Size: 1435 81 335 2144 489
Fragments arranged by size:
2144 1435 489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2851 2851
Size: 4484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 1369 151
Size: 1218 3266
Bpu10I CC'TnA_GC
Cuts at: 337 349 337
Size: 12 4472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1660 2539 3031 3329 1660
Size: 879 492 298 2815
Fragments arranged by size:
2815 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4188 4188
Size: 4484
BsaAI yAC'GTr
Cuts at: 1191 1837 1191
Size: 646 3838
BsaBI GATnn'nnATC
Cuts at: 6 1998 4035 6
Size: 1992 2037 455
Fragments arranged by size:
2037 1992 455
BsaHI Gr'CG_yC
Cuts at: 469 1082 1222 1915 2163 2865 4361 469
Size: 613 140 693 248 702 1496 592
Fragments arranged by size:
1496 702 693 613 592 248 140
BsaWI w'CCGG_w
Cuts at: 1229 1673 2194 2872 3146 3293 3704 1229
Size: 444 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 444 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 227 257 497 527 1012 1042 227
Size: 30 240 30 485 30 3669
Fragments arranged by size:
3669 485 240 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 673 1033 1087 1267 673
Size: 360 54 180 3890
Fragments arranged by size:
3890 360 180 54
BseYI C'CCAG_C
Cuts at: 359 430 702 708 754 996 1123 1333 1909
Size: 71 272 6 46 242 127 210 576
Cuts at: 1909 2667 3244 3740 359
Size: 758 577 496 1103
Fragments arranged by size:
1103 758 577 576 496 272 242 210
127 71 46 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 913
Size: 4484
BsiEI CG_ry'CG
Cuts at: 104 520 577 583 2026 2072 3280 104
Size: 416 57 6 1443 46 1208 1308
Fragments arranged by size:
1443 1308 1208 416 57 46 6
BsiHKAI G_wGCw'C
Cuts at: 665 829 1196 1498 2276 2466 3258 665
Size: 164 367 302 778 190 792 1891
Fragments arranged by size:
1891 792 778 367 302 190 164
BsmI GAATG_Cn'
Cuts at: 282 282
Size: 4484
BsmBI CGTCTCn'nnnn_
Cuts at: 441 441
Size: 4484
Bsp1286I G_dGCh'C
Cuts at: 188 360 665 689 734 829 1196 1265 1498
Size: 172 305 24 45 95 367 69 233
Cuts at: 1498 2109 2202 2276 2466 2528 3258 188
Size: 611 93 74 190 62 730 1414
Fragments arranged by size:
1414 730 611 367 305 233 190 172
95 93 74 69 62 45 24
BspEI T'CCGG_A
Cuts at: 1229 3704 1229
Size: 2475 2009
BspHI T'CATG_A
Cuts at: 3660 3660
Size: 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1387 1534 1748 2050 2431 1387
Size: 147 214 302 381 3440
Fragments arranged by size:
3440 381 302 214 147
BsrBI CCG'CTC
Cuts at: 50 513 2776 2830 4314 4479 50
Size: 463 2263 54 1484 165 55
Fragments arranged by size:
2263 1484 463 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2396 2396
Size: 4484
BsrFI r'CCGG_y
Cuts at: 363 852 2482 2663 2872 363
Size: 489 1630 181 209 1975
Fragments arranged by size:
1975 1630 489 209 181
BssSI C'ACGA_G
Cuts at: 2755 3113 2755
Size: 358 4126
BstAPI GCAn_nnn'nTGC
Cuts at: 1304 1861 1304
Size: 557 3927
BstBI TT'CG_AA
Cuts at: 2845 2845
Size: 4484
BstXI CCAn_nnnn'nTGG
Cuts at: 146 146
Size: 4484
BstYI r'GATC_y
Cuts at: 1 314 1509 1976 1999 2334 2580 3592 3678
Size: 313 1195 467 23 335 246 1012 86
Cuts at: 3678 3690 4036 1
Size: 12 346 449
Fragments arranged by size:
1195 1012 467 449 346 335 313 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 242 401 412 577 1308 107
Size: 135 159 11 165 731 3283
Fragments arranged by size:
3283 731 165 159 135 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2434 2615 2778 2434
Size: 181 163 4140
Fragments arranged by size:
4140 181 163
BtsI GCAGTG_nn'
Cuts at: 1194 1727 1194
Size: 533 3951
ClaI AT'CG_AT
Cuts at: 266 266
Size: 4484
DraI TTT'AAA
Cuts at: 1483 1483
Size: 4484
DrdI GACnn_nn'nnGTC
Cuts at: 594 2190 3048 594
Size: 1596 858 2030
Fragments arranged by size:
2030 1596 858
EaeI y'GGCC_r
Cuts at: 239 574 580 854 2069 2243 2634 2661 4006
Size: 335 6 274 1215 174 391 27 1345
Cuts at: 4006 239
Size: 717
Fragments arranged by size:
1345 1215 717 391 335 274 174 27
6
EagI C'GGCC_G
Cuts at: 574 580 2069 574
Size: 6 1489 2989
Fragments arranged by size:
2989 1489 6
EarI CTCTTCn'nnn_
Cuts at: 1049 2507 2717 1049
Size: 1458 210 2816
Fragments arranged by size:
2816 1458 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 381 2692 3002 3148 4294 381
Size: 2311 310 146 1146 571
Fragments arranged by size:
2311 1146 571 310 146
EcoICRI GAG'CTC
Cuts at: 1496 1496
Size: 4484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 688 1195 1347 1369 2309 2741 3488 3756
Size: 537 507 152 22 940 432 747 268
Cuts at: 3756 151
Size: 879
Fragments arranged by size:
940 879 747 537 507 432 268 152
22
EcoNI CCTnn'n_nnAGG
Cuts at: 1054 1054
Size: 4484
EcoO109I rG'GnC_Cy
Cuts at: 356 1047 1238 1353 1623 356
Size: 691 191 115 270 3217
Fragments arranged by size:
3217 691 270 191 115
EcoRI G'AATT_C
Cuts at: 1488 1488
Size: 4484
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2503 2503
Size: 4484
FspI TGC'GCA
Cuts at: 2265 2265
Size: 4484
HaeII r_GCGC'y
Cuts at: 336 472 491 807 997 1085 1918 2166 3188
Size: 136 19 316 190 88 833 248 1022
Cuts at: 3188 4167 336
Size: 979 653
Fragments arranged by size:
1022 979 833 653 316 248 190 136
88 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 497 509 529 541 2326 2356 2358 2388 497
Size: 12 20 12 1785 30 2 30 2593
Fragments arranged by size:
2593 1785 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 1523 1523
Size: 4484
KasI G'GCGC_C
Cuts at: 468 1081 1914 2162 468
Size: 613 833 248 2790
Fragments arranged by size:
2790 833 613 248
KpnI G_GTAC'C
Cuts at: 1504 1504
Size: 4484
MluI A'CGCG_T
Cuts at: 1004 3917 1004
Size: 2913 1571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 540 3155 3339 540
Size: 2615 184 1685
Fragments arranged by size:
2615 1685 184
MscI TGG'CCA
Cuts at: 2245 2245
Size: 4484
MslI CAynn'nnrTG
Cuts at: 1313 2473 2600 1313
Size: 1160 127 3197
Fragments arranged by size:
3197 1160 127
MspA1I CmG'CkG
Cuts at: 403 414 481 522 546 579 870 901 1127
Size: 11 67 41 24 33 291 31 226
Cuts at: 1127 1367 1595 1909 2269 3282 3527 403
Size: 240 228 314 360 1013 245 1360
Fragments arranged by size:
1360 1013 360 314 291 245 240 228
226 67 41 33 31 24 11
NaeI GCC'GGC
Cuts at: 854 2665 854
Size: 1811 2673
NarI GG'CG_CC
Cuts at: 469 1082 1915 2163 469
Size: 613 833 248 2790
Fragments arranged by size:
2790 833 613 248
NcoI C'CATG_G
Cuts at: 107 1308 107
Size: 1201 3283
NgoMIV G'CCGG_C
Cuts at: 852 2663 852
Size: 1811 2673
NotI GC'GGCC_GC
Cuts at: 574 574
Size: 4484
NspI r_CATG'y
Cuts at: 1537 2037 2944 1537
Size: 500 907 3077
Fragments arranged by size:
3077 907 500
PasI CC'CwG_GG
Cuts at: 776 1356 776
Size: 580 3904
PciI A'CATG_T
Cuts at: 2940 2940
Size: 4484
PfoI T'CCnGG_A
Cuts at: 1049 1346 1049
Size: 297 4187
PmeI GTTT'AAAC
Cuts at: 1483 1483
Size: 4484
PmlI CAC'GTG
Cuts at: 1191 1191
Size: 4484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 3623
Size: 32 4452
PpuMI rG'GwC_Cy
Cuts at: 1047 1047
Size: 4484
PspOMI G'GGCC_C
Cuts at: 356 356
Size: 4484
PstI C_TGCA'G
Cuts at: 875 1531 2216 875
Size: 656 685 3143
Fragments arranged by size:
3143 685 656
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4484
PvuII CAG'CTG
Cuts at: 901 1127 1367 1909 2269 901
Size: 226 240 542 360 3116
Fragments arranged by size:
3116 542 360 240 226
RsrII CG'GwC_CG
Cuts at: 2679 2679
Size: 4484
SacI G_AGCT'C
Cuts at: 1498 1498
Size: 4484
SacII CC_GC'GG
Cuts at: 404 415 580 404
Size: 11 165 4308
Fragments arranged by size:
4308 165 11
SalI G'TCGA_C
Cuts at: 1521 1521
Size: 4484
SapI GCTCTTCn'nnn_
Cuts at: 2507 2717 2507
Size: 210 4274
SbfI CC_TGCA'GG
Cuts at: 1531 1531
Size: 4484
ScaI AGT'ACT
Cuts at: 4001 4001
Size: 4484
SexAI A'CCwGG_T
Cuts at: 986 986
Size: 4484
SfcI C'TryA_G
Cuts at: 33 871 1527 2212 3205 3396 33
Size: 838 656 685 993 191 1121
Fragments arranged by size:
1121 993 838 685 656 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 238 238
Size: 4484
SfoI GGC'GCC
Cuts at: 470 1083 1916 2164 470
Size: 613 833 248 2790
Fragments arranged by size:
2790 833 613 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4484
SmaI CCC'GGG
Cuts at: 1506 1506
Size: 4484
SmlI C'TyrA_G
Cuts at: 1639 2554 3046 3308 1639
Size: 915 492 262 2815
Fragments arranged by size:
2815 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4484
SphI G_CATG'C
Cuts at: 1537 2037 1537
Size: 500 3984
StuI AGG'CCT
Cuts at: 388 1446 388
Size: 1058 3426
StyI C'CwwG_G
Cuts at: 107 145 1308 1618 1972 107
Size: 38 1163 310 354 2619
Fragments arranged by size:
2619 1163 354 310 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 235 243 444 1137 2067 2200 2659 2868 235
Size: 8 201 693 930 133 459 209 1851
Fragments arranged by size:
1851 930 693 459 209 201 133 8
TatI w'GTAC_w
Cuts at: 3999 3999
Size: 4484
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 227 259 1012 1044 4001 4033 227
Size: 32 753 32 2957 32 678
Fragments arranged by size:
2957 753 678 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2281 2281
Size: 4484
XbaI T'CTAG_A
Cuts at: 1515 1515
Size: 4484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1979 1979
Size: 4484
XmaI C'CCGG_G
Cuts at: 1504 1504
Size: 4484
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AleI AloI AscI AvrII BaeI BbsI
BfrBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PflMI PshAI PsiI
PspXI PsrI SanDI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI